Restriction map for
pFN21AB9904
(Linear) (Six-base) MAPSORT of: KIBB9904.seq Check: 314 from: 1 to: 1767
KIBB9904.orf
With 182 enzymes: SgfI *
March 2, 2010 14:30 ..
AccI GT'mk_AC
Cuts at: 0 1295 1767
Size: 1295 472
Acc65I G'GTAC_C
Cuts at: 0 968 1767
Size: 968 799
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 1413 1753 1767
Size: 808 605 340 14
Fragments arranged by size:
808 605 340 14
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1522 1767
Size: 1522 245
AlwNI CAG_nnn'CTG
Cuts at: 0 1344 1767
Size: 1344 423
ApaI G_GGCC'C
Cuts at: 0 171 959 1004 1066 1767
Size: 171 788 45 62 701
Fragments arranged by size:
788 701 171 62 45
ApoI r'AATT_y
Cuts at: 0 709 1237 1767
Size: 709 528 530
Fragments arranged by size:
709 530 528
AvaI C'yCGr_G
Cuts at: 0 20 1117 1767
Size: 20 1097 650
Fragments arranged by size:
1097 650 20
BanI G'GyrC_C
Cuts at: 0 968 1091 1767
Size: 968 123 676
Fragments arranged by size:
968 676 123
BanII G_rGCy'C
Cuts at: 0 171 959 1004 1066 1767
Size: 171 788 45 62 701
Fragments arranged by size:
788 701 171 62 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1188 1197 1409 1767
Size: 1188 9 212 358
Fragments arranged by size:
1188 358 212 9
BciVI GTATCCnnnnn_n'
Cuts at: 0 834 1767
Size: 834 933
BclI T'GATC_A
Cuts at: 0 834 1767
Size: 834 933
BglI GCCn_nnn'nGGC
Cuts at: 0 244 736 1767
Size: 244 492 1031
Fragments arranged by size:
1031 492 244
BglII A'GATC_T
Cuts at: 0 533 1556 1767
Size: 533 1023 211
Fragments arranged by size:
1023 533 211
BlpI GC'TnA_GC
Cuts at: 0 1754 1767
Size: 1754 13
Bme1580I G_kGCm'C
Cuts at: 0 171 732 959 1004 1066 1094 1767
Size: 171 561 227 45 62 28 673
Fragments arranged by size:
673 561 227 171 62 45 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 298 1077 1767
Size: 298 779 690
Fragments arranged by size:
779 690 298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1641 1767
Size: 1641 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 1549 1660 1687 1767
Size: 67 1482 111 27 80
Fragments arranged by size:
1482 111 80 67 27
Bpu10I CC'TnA_GC
Cuts at: 0 173 1767
Size: 173 1594
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 1767
Size: 166 1601
BsaHI Gr'CG_yC
Cuts at: 0 36 1767
Size: 36 1731
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 1767
Size: 80 30 1657
Fragments arranged by size:
1657 80 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 248 1555 1617 1767
Size: 248 1307 62 150
Fragments arranged by size:
1307 248 150 62
BseYI C'CCAG_C
Cuts at: 0 501 731 1495 1569 1767
Size: 501 230 764 74 198
Fragments arranged by size:
764 501 230 198 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1492 1767
Size: 1492 275
BsiEI CG_ry'CG
Cuts at: 0 85 165 1767
Size: 85 80 1602
Fragments arranged by size:
1602 85 80
BsiHKAI G_wGCw'C
Cuts at: 0 1151 1767
Size: 1151 616
Bsp1286I G_dGCh'C
Cuts at: 0 171 732 959 1004 1066 1094 1151 1767
Size: 171 561 227 45 62 28 57 616
Fragments arranged by size:
616 561 227 171 62 57 45 28
BspHI T'CATG_A
Cuts at: 0 1241 1767
Size: 1241 526
BsrDI GCAATG_nn'
Cuts at: 0 488 1227 1767
Size: 488 739 540
Fragments arranged by size:
739 540 488
BsrFI r'CCGG_y
Cuts at: 0 196 1767
Size: 196 1571
BstEII G'GTnAC_C
Cuts at: 0 496 880 1051 1533 1767
Size: 496 384 171 482 234
Fragments arranged by size:
496 482 384 234 171
BstYI r'GATC_y
Cuts at: 0 533 1556 1767
Size: 533 1023 211
Fragments arranged by size:
1023 533 211
BtgI C'CryG_G
Cuts at: 0 620 960 1265 1767
Size: 620 340 305 502
Fragments arranged by size:
620 502 340 305
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 106 715 1577 1673 1767
Size: 106 609 862 96 94
Fragments arranged by size:
862 609 106 96 94
BtsI GCAGTG_nn'
Cuts at: 0 159 464 1748 1767
Size: 159 305 1284 19
Fragments arranged by size:
1284 305 159 19
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 852 887 1767
Size: 852 35 880
Fragments arranged by size:
880 852 35
EaeI y'GGCC_r
Cuts at: 0 132 162 1582 1767
Size: 132 30 1420 185
Fragments arranged by size:
1420 185 132 30
EagI C'GGCC_G
Cuts at: 0 162 1767
Size: 162 1605
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 134 1767
Size: 40 94 1633
Fragments arranged by size:
1633 94 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 808 1413 1549 1660 1687 1753 1767
Size: 67 741 605 136 111 27 66 14
Fragments arranged by size:
741 605 136 111 67 66 27 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 1767
Size: 355 1412
EcoO109I rG'GnC_Cy
Cuts at: 0 168 321 955 956 1001 1062 1767
Size: 168 153 634 1 45 61 705
Fragments arranged by size:
705 634 168 153 61 45 1
EcoRV GAT'ATC
Cuts at: 0 765 1767
Size: 765 1002
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 367 1767
Size: 367 1400
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1633 1665 1767
Size: 1633 32 102
Fragments arranged by size:
1633 102 32
HincII GTy'rAC
Cuts at: 0 513 1767
Size: 513 1254
KpnI G_GTAC'C
Cuts at: 0 972 1767
Size: 972 795
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 1767
Size: 1379 388
MslI CAynn'nnrTG
Cuts at: 0 983 1767
Size: 983 784
MspA1I CmG'CkG
Cuts at: 0 75 735 1527 1767
Size: 75 660 792 240
Fragments arranged by size:
792 660 240 75
NaeI GCC'GGC
Cuts at: 0 198 1767
Size: 198 1569
NcoI C'CATG_G
Cuts at: 0 1265 1767
Size: 1265 502
NgoMIV G'CCGG_C
Cuts at: 0 196 1767
Size: 196 1571
PasI CC'CwG_GG
Cuts at: 0 544 1767
Size: 544 1223
PfoI T'CCnGG_A
Cuts at: 0 562 1438 1767
Size: 562 876 329
Fragments arranged by size:
876 562 329
PspOMI G'GGCC_C
Cuts at: 0 167 955 1000 1062 1767
Size: 167 788 45 62 705
Fragments arranged by size:
788 705 167 62 45
SfcI C'TryA_G
Cuts at: 0 1292 1767
Size: 1292 475
SgrAI Cr'CCGG_yG
Cuts at: 0 196 1767
Size: 196 1571
SmaI CCC'GGG
Cuts at: 0 22 1767
Size: 22 1745
SmlI C'TyrA_G
Cuts at: 0 181 1767
Size: 181 1586
StuI AGG'CCT
Cuts at: 0 361 745 1767
Size: 361 384 1022
Fragments arranged by size:
1022 384 361
StyI C'CwwG_G
Cuts at: 0 740 1265 1767
Size: 740 525 502
Fragments arranged by size:
740 525 502
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 515 1205 1552 1767
Size: 515 690 347 215
Fragments arranged by size:
690 515 347 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 1767
Size: 80 32 1655
Fragments arranged by size:
1655 80 32
Tth111I GACn'n_nGTC
Cuts at: 0 1192 1767
Size: 1192 575
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 387 1272 1767
Size: 387 885 495
Fragments arranged by size:
885 495 387
XmaI C'CCGG_G
Cuts at: 0 20 1767
Size: 20 1747
XmnI GAAnn'nnTTC
Cuts at: 0 823 1600 1767
Size: 823 777 167
Fragments arranged by size:
823 777 167
Enzymes that do cut:
AccI Acc65I AcuI AlfI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspHI BsrDI
BsrFI BstEII BstYI BtgI BtgZI BtsI CspCI EaeI
EagI EciI Eco57MI EcoNI EcoO109I EcoRV FalI Hin4I
HincII KpnI MmeI MslI MspA1I NaeI NcoI NgoMIV
PasI PfoI PspOMI SfcI SgrAI SmaI SmlI StuI
StyI TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BcgI BfrBI BmgBI BmtI BsaI
BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I
Bsu36I ClaI DraI DraIII DrdI EarI EcoICRI EcoRI
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MscI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI ZraI