Restriction map for
pFN21AE0975
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# NO CUT ENZYMES ( 109 enzymes) #
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AccI AcsI AflII AgeI AhdI
Aor51HI ApoI AscI AseI AsnI
Asp700I AspEI AspI AviII BamHI
BbeI BbsI BcgI BclI BfrI
BglII BlpI Bpu1102I BpuAI BsaBI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BsrDI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MflI MluI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshAI PshBI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AatII 331 GACGTC GACGT^C
Acc65I 545 GGTACC G^GTACC
AclI 1225 AACGTT AA^CGTT
AcyI 331 GACGTC G[GA]^CG[CT]C
AflIII 599 ACATGT A^C[GA][CT]GT
ApaI 1068 GGGCCC GGGCC^C
Asp718I 545 GGTACC G^GTACC
AvrII 1269 CCTAGG C^CTAGG
BanII 1068 GGGCCC G[GA]GC[CT]^C
BbrPI 337 CACGTG CAC^GTG
BbvCI 1123 CCTCAGC CCTCAGC(-5/-2)
BciVI 617 GTATCC GTATCC(6/5)
BglI 1390 GCCAACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1269 CCTAGG C^CTAGG
BsaHI 331 GACGTC G[GA]^CG[CT]C
BsiEI 1491 CGGCCG CG[GA][CT]^CG
BspLU11I 599 ACATGT A^CATGT
BstAPI 393 GCACGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 192 CCAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EagI 1491 CGGCCG C^GGCCG
EarI 570 CTCTTC CTCTTC(1/4)
EclXI 1491 CGGCCG C^GGCCG
Eco52I 1491 CGGCCG C^GGCCG
HaeII 1305 AGCGCC [GA]GCGC^[CT]
Hin1I 331 GACGTC G[GA]^CG[CT]C
KpnI 545 GGTACC GGTAC^C
Ksp632I 570 CTCTTC CTCTTC(1/4)
MseI 877 TTAA T^TAA
MslI 6 CACCGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 599 ACATGT [GA]CATG^[CT]
PaeR7I 1056 CTCGAG C^TCGAG
PmaCI 337 CACGTG CAC^GTG
PmlI 337 CACGTG CAC^GTG
Psp1406I 1225 AACGTT AA^CGTT
SexAI 266 ACCTGGT A^CC[AT]GGT
SfiI 1389 GGCCAACCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 965 CCCGGG CCC^GGG
SmlI 1056 CTCGAG C^T[CT][GA]AG
StuI 827 AGGCCT AGG^CCT
TaqI 1057 TCGA T^CGA
Tru9I 877 TTAA T^TAA
TthHB8I 1057 TCGA T^CGA
XcmI 422 CCAATGTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1056 CTCGAG C^TCGAG
XmaCI 965 CCCGGG C^CCGGG
XmaI 965 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 882 CGCG CG^CG
1012 CGCG
Alw44I 3 GTGCAC G^TGCAC
400 GTGCAC
ApaLI 3 GTGCAC G^TGCAC
400 GTGCAC
BfaI 1136 CTAG C^TAG
1270 CTAG
BsaI 642 GAGACC GGTCTC(1/5)
694 GAGACC
BseRI 187 CTCCTC GAGGAG(10/8)
715 GAGGAG
BspMI 1100 ACCTGC ACCTGC(4/8)
1604 ACCTGC
BsrBI 477 CCGCTC CCGCTC(-3/-3)
1244 CCGCTC
BssSI 944 CACGAG CACGAG(-5/-1)
1201 CACGAG
BstUI 882 CGCG CG^CG
1012 CGCG
DraIII 394 CACGTTGTG CAC[ACGT][ACGT][ACGT]^GTG
1624 CACCATGTG
HgaI 789 GACGC GACGC(5/10)
1013 GCGTC
MaeI 1136 CTAG C^TAG
1270 CTAG
MboII 350 TCTTC GAAGA(8/7)
571 TCTTC
MvnI 882 CGCG CG^CG
1012 CGCG
PflMI 536 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCACCAGCTGG
PstI 325 CTGCAG CTGCA^G
895 CTGCAG
SfcI 325 CTGCAG C^T[GA][CT]AG
895 CTGCAG
Van91I 536 CCACACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCACCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BbvI BcnI BmyI
BpmI BsaAI BsaJI BsaWI BseAI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspEI BsrI
BssKI BstF5I BstNI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MluNI MnlI MroI MscI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI Tsp45I TspRI