(Circular) (Six-base) MAPSORT of: pFN21AE0975.seq Check: 242 from: 1 to: 6337 pFN21AE0975 6337 bp With 182 enzymes: SgfI * April 15, 2010 09:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3114 3618 3114 Size: 504 5833 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2340 4147 278 Size: 53 83 186 936 804 1807 2468 Fragments arranged by size: 2468 1807 936 804 186 83 53 AccI GT'mk_AC Cuts at: 1937 3680 1937 Size: 1743 4594 Acc65I G'GTAC_C Cuts at: 2550 3658 2550 Size: 1108 5229 AclI AA'CG_TT Cuts at: 1563 3231 4464 4837 1563 Size: 1668 1233 373 3063 Fragments arranged by size: 3063 1668 1233 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4400 5784 6052 1632 Size: 2768 1384 268 1917 Fragments arranged by size: 2768 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6140 AflIII A'CryG_T Cuts at: 1203 2604 5236 6213 1203 Size: 1401 2632 977 1327 Fragments arranged by size: 2632 1401 1327 977 AgeI A'CCGG_T Cuts at: 5168 5168 Size: 6337 AhdI GACnn_n'nnGTC Cuts at: 5066 5066 Size: 6337 AleI CACnn'nnGTG Cuts at: 1166 2015 1166 Size: 849 5488 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6337 AlwNI CAG_nnn'CTG Cuts at: 2368 2797 2899 3406 4080 5652 2368 Size: 429 102 507 674 1572 3053 Fragments arranged by size: 3053 1572 674 507 429 102 ApaI G_GGCC'C Cuts at: 3077 3077 Size: 6337 ApaLI G'TGCA_C Cuts at: 2008 2405 4394 5550 2008 Size: 397 1989 1156 2795 Fragments arranged by size: 2795 1989 1156 397 ApoI r'AATT_y Cuts at: 1493 3646 3872 4024 6133 1493 Size: 2153 226 152 2109 1697 Fragments arranged by size: 2153 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4891 160 Size: 872 3859 1606 Fragments arranged by size: 3859 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2392 2970 3061 3662 1870 Size: 73 117 332 578 91 601 4545 Fragments arranged by size: 4545 601 578 332 117 91 73 AvrII C'CTAG_G Cuts at: 3274 3274 Size: 6337 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2534 2567 2534 Size: 33 6304 BamHI G'GATC_C Cuts at: 3667 4034 3667 Size: 367 5970 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2550 3056 3530 3542 3627 Size: 325 217 627 763 506 474 12 85 Cuts at: 3627 3658 5113 618 Size: 31 1455 1842 Fragments arranged by size: 1842 1455 763 627 506 474 325 217 85 31 12 BanII G_rGCy'C Cuts at: 1391 3077 3656 1391 Size: 1686 579 4072 Fragments arranged by size: 4072 1686 579 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5366 BbvCI CC'TCA_GC Cuts at: 3129 3129 Size: 6337 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4528 4562 1903 Size: 34 2591 34 3678 Fragments arranged by size: 3678 2591 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 4229 5439 2633 Size: 1596 1210 3531 Fragments arranged by size: 3531 1596 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6337 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3401 4948 136 Size: 107 122 71 631 605 1729 1547 1525 Fragments arranged by size: 1729 1547 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6332 6332 Size: 6337 BlpI GC'TnA_GC Cuts at: 3754 3754 Size: 6337 Bme1580I G_kGCm'C Cuts at: 1790 2012 2400 2409 2950 2977 3077 3535 3630 Size: 222 388 9 541 27 100 458 95 Cuts at: 3630 4398 5554 1790 Size: 768 1156 2573 Fragments arranged by size: 2573 1156 768 541 458 388 222 100 95 27 9 BmgBI CAC'GTC Cuts at: 1318 2239 2667 1318 Size: 921 428 4988 Fragments arranged by size: 4988 921 428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3611 4068 5026 Size: 416 53 83 399 24 2194 457 958 Cuts at: 5026 442 Size: 1753 Fragments arranged by size: 2194 1753 958 457 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6337 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3103 3103 Size: 6337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2705 2717 3056 3641 4997 1239 Size: 941 525 12 339 585 1356 2579 Fragments arranged by size: 2579 1356 941 585 525 339 12 Bpu10I CC'TnA_GC Cuts at: 2743 3129 3387 2743 Size: 386 258 5693 Fragments arranged by size: 5693 386 258 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4462 5327 5625 4462 Size: 865 298 5174 Fragments arranged by size: 5174 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2641 2693 5000 882 Size: 617 1142 52 2307 2219 Fragments arranged by size: 2307 2219 1142 617 52 BsaAI yAC'GTr Cuts at: 493 1204 2344 2536 2914 4128 493 Size: 711 1140 192 378 1214 2702 Fragments arranged by size: 2702 1214 1140 711 378 192 BsaBI GATnn'nnATC Cuts at: 6331 6331 Size: 6337 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2337 4144 4526 5161 Size: 53 83 186 936 804 1807 382 635 Cuts at: 5161 275 Size: 1451 Fragments arranged by size: 1807 1451 936 804 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 2100 2313 2560 3481 4770 5168 5442 5589 Size: 66 213 247 921 1289 398 274 147 Cuts at: 5589 6000 2034 Size: 411 2371 Fragments arranged by size: 2371 1289 921 411 398 274 247 213 147 66 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2095 2125 2333 2363 1178 Size: 30 165 30 692 30 208 30 5152 Fragments arranged by size: 5152 692 208 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2183 2735 1186 Size: 997 552 4788 Fragments arranged by size: 4788 997 552 BseYI C'CCAG_C Cuts at: 2773 2933 5540 6036 2773 Size: 160 2607 496 3074 Fragments arranged by size: 3074 2607 496 160 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2434 2750 2936 3092 3173 3240 1163 Size: 573 698 316 186 156 81 67 4260 Fragments arranged by size: 4260 698 573 316 186 156 81 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3499 3787 4548 5576 1540 Size: 275 186 1498 288 761 1028 2301 Fragments arranged by size: 2301 1498 1028 761 288 275 186 BsiHKAI G_wGCw'C Cuts at: 2012 2409 2436 2560 3207 4398 4483 5554 2012 Size: 397 27 124 647 1191 85 1071 2795 Fragments arranged by size: 2795 1191 1071 647 397 124 85 27 BsmI GAATG_Cn' Cuts at: 3848 3941 3848 Size: 93 6244 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2400 2409 2436 2560 2950 2977 Size: 399 222 388 9 27 124 390 27 Cuts at: 2977 3077 3207 3535 3630 3656 4398 4483 5554 Size: 100 130 328 95 26 742 85 1071 Cuts at: 5554 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 390 388 328 222 130 124 100 95 85 27 27 26 9 BspEI T'CCGG_A Cuts at: 2034 2313 3481 6000 2034 Size: 279 1168 2519 2371 Fragments arranged by size: 2519 2371 1168 279 BspHI T'CATG_A Cuts at: 4226 5956 4226 Size: 1730 4607 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3114 3618 3692 844 Size: 2270 504 74 3489 Fragments arranged by size: 3489 2270 504 74 BsrBI CCG'CTC Cuts at: 1394 1823 2484 3251 4224 1394 Size: 429 661 767 973 3507 Fragments arranged by size: 3507 973 767 661 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4832 5006 65 Size: 1183 3584 174 1396 Fragments arranged by size: 3584 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4981 5168 1700 Size: 110 3171 187 2869 Fragments arranged by size: 3171 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6337 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6337 BssSI C'ACGA_G Cuts at: 2949 3206 4397 5409 2949 Size: 257 1191 1012 3877 Fragments arranged by size: 3877 1191 1012 257 BstAPI GCAn_nnn'nTGC Cuts at: 794 2404 794 Size: 1610 4727 BstBI TT'CG_AA Cuts at: 5141 5141 Size: 6337 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6337 BstXI CCAn_nnnn'nTGG Cuts at: 2204 2204 Size: 6337 BstYI r'GATC_y Cuts at: 1972 3667 4034 4419 4436 5888 5974 5986 6332 Size: 1695 367 385 17 1452 86 12 346 Cuts at: 6332 1972 Size: 1977 Fragments arranged by size: 1977 1695 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2502 2511 2846 2502 Size: 9 335 5993 Fragments arranged by size: 5993 335 9 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5785 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5598 Fragments arranged by size: 5598 683 56 BtsI GCAGTG_nn' Cuts at: 2100 2897 2926 3853 4647 4667 2100 Size: 797 29 927 794 20 3770 Fragments arranged by size: 3770 927 797 794 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6337 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6302 DraI TTT'AAA Cuts at: 3641 3641 Size: 6337 DraIII CAC_nnn'GTG Cuts at: 2404 3634 2404 Size: 1230 5107 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5344 809 Size: 507 4028 1802 Fragments arranged by size: 4028 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2538 2783 Size: 54 1425 50 275 18 291 417 245 Cuts at: 2783 3393 3447 3496 3784 4673 6302 8 Size: 610 54 49 288 889 1629 43 Fragments arranged by size: 1629 1425 889 610 417 291 288 275 245 54 54 50 49 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3496 3784 1537 Size: 275 1684 288 4090 Fragments arranged by size: 4090 1684 288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2581 4267 1353 Size: 1228 1686 3423 Fragments arranged by size: 3423 1686 1228 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4924 5298 5444 387 Size: 1064 138 3335 374 146 1280 Fragments arranged by size: 3335 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2705 2717 3056 3641 4400 4997 Size: 393 548 525 12 339 585 759 597 Cuts at: 4997 5784 6052 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 759 597 585 548 525 393 339 268 12 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2501 3620 2330 Size: 171 1119 5047 Fragments arranged by size: 5047 1119 171 EcoO109I rG'GnC_Cy Cuts at: 2070 2507 2725 2778 3073 3158 3171 3291 3403 Size: 437 218 53 295 85 13 120 112 Cuts at: 3403 3463 2070 Size: 60 4944 Fragments arranged by size: 4944 437 295 218 120 112 85 60 53 13 EcoRI G'AATT_C Cuts at: 3646 4024 3646 Size: 378 5959 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 6337 FspI TGC'GCA Cuts at: 4843 4843 Size: 6337 HaeII r_GCGC'y Cuts at: 1678 3314 5484 1678 Size: 1636 2170 2531 Fragments arranged by size: 2531 2170 1636 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2031 2063 3194 3226 4978 Size: 32 135 32 459 32 1131 32 1752 Cuts at: 4978 5010 5052 5084 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1752 1131 459 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3681 3927 669 Size: 988 281 1743 246 3079 Fragments arranged by size: 3079 1743 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6337 HpaI GTT'AAC Cuts at: 3927 3927 Size: 6337 KpnI G_GTAC'C Cuts at: 2554 3662 2554 Size: 1108 5229 MfeI C'AATT_G Cuts at: 3936 3936 Size: 6337 MluI A'CGCG_T Cuts at: 6213 6213 Size: 6337 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5451 5635 1305 Size: 4146 184 2007 Fragments arranged by size: 4146 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2123 2540 3395 3449 10 Size: 54 1425 343 291 417 855 54 2898 Fragments arranged by size: 2898 1425 855 417 343 291 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2015 4295 4654 4813 518 Size: 648 849 2280 359 159 2042 Fragments arranged by size: 2280 2042 849 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2683 2773 3054 3133 3753 4430 5578 5823 Size: 1077 90 281 79 620 677 1148 245 Cuts at: 5823 1606 Size: 2120 Fragments arranged by size: 2120 1148 1077 677 620 281 245 90 79 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5785 NdeI CA'TA_TG Cuts at: 387 387 Size: 6337 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6337 NotI GC'GGCC_GC Cuts at: 3784 3784 Size: 6337 NspI r_CATG'y Cuts at: 1132 1229 2608 3695 5240 1132 Size: 97 1379 1087 1545 2229 Fragments arranged by size: 2229 1545 1379 1087 97 PasI CC'CwG_GG Cuts at: 1348 2488 3296 3535 1348 Size: 1140 808 239 4150 Fragments arranged by size: 4150 1140 808 239 PciI A'CATG_T Cuts at: 2604 5236 2604 Size: 2632 3705 PflMI CCAn_nnn'nTGG Cuts at: 1108 2547 2683 1108 Size: 1439 136 4762 Fragments arranged by size: 4762 1439 136 PfoI T'CCnGG_A Cuts at: 2178 2196 2436 2694 3033 2178 Size: 18 240 258 339 5482 Fragments arranged by size: 5482 339 258 240 18 PmeI GTTT'AAAC Cuts at: 3641 3641 Size: 6337 PmlI CAC'GTG Cuts at: 2344 2344 Size: 6337 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4406 4438 5919 5951 4406 Size: 32 1481 32 4792 Fragments arranged by size: 4792 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 2778 3158 3463 2725 Size: 53 380 305 5599 Fragments arranged by size: 5599 380 305 53 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6337 PsiI TTA'TAA Cuts at: 3907 3907 Size: 6337 PspOMI G'GGCC_C Cuts at: 3073 3073 Size: 6337 PstI C_TGCA'G Cuts at: 830 2334 2904 3689 830 Size: 1504 570 785 3478 Fragments arranged by size: 3478 1504 785 570 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6337 PvuII CAG'CTG Cuts at: 2683 2773 3133 2683 Size: 90 360 5887 Fragments arranged by size: 5887 360 90 SalI G'TCGA_C Cuts at: 1936 3679 1936 Size: 1743 4594 SanDI GG'GwC_CC Cuts at: 2778 3158 2778 Size: 380 5957 SbfI CC_TGCA'GG Cuts at: 3689 3689 Size: 6337 ScaI AGT'ACT Cuts at: 4585 6297 4585 Size: 1712 4625 SexAI A'CCwGG_T Cuts at: 2271 2271 Size: 6337 SfcI C'TryA_G Cuts at: 826 1045 2330 2900 3685 4820 5501 5692 826 Size: 219 1285 570 785 1135 681 191 1471 Fragments arranged by size: 1471 1285 1135 785 681 570 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3401 3401 Size: 6337 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6337 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6337 SmaI CCC'GGG Cuts at: 1872 2972 3664 1872 Size: 1100 692 4545 Fragments arranged by size: 4545 1100 692 SmlI C'TyrA_G Cuts at: 820 1017 1943 3061 4441 5342 5604 820 Size: 197 926 1118 1380 901 262 1553 Fragments arranged by size: 1553 1380 1118 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4128 493 Size: 3635 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6337 SphI G_CATG'C Cuts at: 1132 3695 1132 Size: 2563 3774 SspI AAT'ATT Cuts at: 5 52 4261 5 Size: 47 4209 2081 Fragments arranged by size: 4209 2081 47 StuI AGG'CCT Cuts at: 1517 2834 1517 Size: 1317 5020 StyI C'CwwG_G Cuts at: 513 1065 2593 2835 3274 3776 513 Size: 552 1528 242 439 502 3074 Fragments arranged by size: 3074 1528 552 502 439 242 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4364 4381 4534 4719 5164 325 Size: 1255 2784 17 153 185 445 1498 Fragments arranged by size: 2784 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4583 6295 96 Size: 274 80 33 51 1443 2606 1712 138 Fragments arranged by size: 2606 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2095 2127 2333 2365 6297 6329 2095 Size: 32 206 32 3932 32 2103 Fragments arranged by size: 3932 2103 206 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6337 XbaI T'CTAG_A Cuts at: 3673 3673 Size: 6337 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2434 2434 Size: 6337 XhoI C'TCGA_G Cuts at: 1943 3061 1943 Size: 1118 5219 XmaI C'CCGG_G Cuts at: 1870 2970 3662 1870 Size: 1100 692 4545 Fragments arranged by size: 4545 1100 692 XmnI GAAnn'nnTTC Cuts at: 4466 4466 Size: 6337 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2338 4145 276 Size: 53 83 186 936 804 1807 2468 Fragments arranged by size: 2468 1807 936 804 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI