Restriction map for
pFN21AB8178
(Circular) (Six-base) MAPSORT of: pFN21AB8178.seq Check: 5531 from: 1 to: 6010
pFN21AB8178 6010 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3820 278
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3110 3353 1937
Size: 1173 243 4594
Fragments arranged by size:
4594 1173 243
Acc65I G'GTAC_C
Cuts at: 2948 3331 2948
Size: 383 5627
AclI AA'CG_TT
Cuts at: 1563 2740 4137 4510 1563
Size: 1177 1397 373 3063
Fragments arranged by size:
3063 1397 1177 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4073 5457 5725 1632
Size: 2441 1384 268 1917
Fragments arranged by size:
2441 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5813
AflIII A'CryG_T
Cuts at: 1203 2782 3105 4909 5886 1203
Size: 1579 323 1804 977 1327
Fragments arranged by size:
1804 1579 1327 977 323
AgeI A'CCGG_T
Cuts at: 4841 4841
Size: 6010
AhdI GACnn_n'nnGTC
Cuts at: 2441 3199 4739 2441
Size: 758 1540 3712
Fragments arranged by size:
3712 1540 758
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6010
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6010
AlwNI CAG_nnn'CTG
Cuts at: 2044 2369 2581 2639 3753 5325 2044
Size: 325 212 58 1114 1572 2729
Fragments arranged by size:
2729 1572 1114 325 212 58
ApaLI G'TGCA_C
Cuts at: 4067 5223 4067
Size: 1156 4854
ApoI r'AATT_y
Cuts at: 1493 2060 2383 3074 3319 3545 3697 5806 1493
Size: 567 323 691 245 226 152 2109 1697
Fragments arranged by size:
2109 1697 691 567 323 245 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4564 160
Size: 872 3532 1606
Fragments arranged by size:
3532 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
AvaI C'yCGr_G
Cuts at: 1870 1943 3335 1870
Size: 73 1392 4545
Fragments arranged by size:
4545 1392 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2932 2965 2932
Size: 33 5977
BamHI G'GATC_C
Cuts at: 2792 3340 3707 2792
Size: 548 367 5095
Fragments arranged by size:
5095 548 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2948 3331 4786 618
Size: 325 217 627 1161 383 1455 1842
Fragments arranged by size:
1842 1455 1161 627 383 325 217
BanII G_rGCy'C
Cuts at: 1391 3276 3329 1391
Size: 1885 53 4072
Fragments arranged by size:
4072 1885 53
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5039
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4201 4235 1903
Size: 34 2264 34 3678
Fragments arranged by size:
3678 2264 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3286 3902 5112 3286
Size: 616 1210 4184
Fragments arranged by size:
4184 1210 616
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6010
BfrBI ATG'CAT
Cuts at: 2184 3164 2184
Size: 980 5030
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2576 4621 136
Size: 107 122 71 631 605 904 2045 1525
Fragments arranged by size:
2045 1525 904 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2400 6005 2400
Size: 3605 2405
BlpI GC'TnA_GC
Cuts at: 2894 3427 2894
Size: 533 5477
Bme1580I G_kGCm'C
Cuts at: 1790 4071 5227 1790
Size: 2281 1156 2573
Fragments arranged by size:
2573 2281 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6010
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2497 2597 3741
Size: 416 53 83 399 24 1080 100 1144
Cuts at: 3741 4699 442
Size: 958 1753
Fragments arranged by size:
1753 1144 1080 958 416 399 100 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2021 2459 2603 4670 1239
Size: 782 438 144 2067 2579
Fragments arranged by size:
2579 2067 782 438 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4135 5000 5298 4135
Size: 865 298 4847
Fragments arranged by size:
4847 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4673 882
Size: 617 3174 2219
Fragments arranged by size:
3174 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3801 493
Size: 711 2597 2702
Fragments arranged by size:
2702 2597 711
BsaBI GATnn'nnATC
Cuts at: 6004 6004
Size: 6010
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3817 4199 4834 275
Size: 53 83 186 936 2284 382 635 1451
Fragments arranged by size:
2284 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4443 4841 5115 5262 5673 4443
Size: 398 274 147 411 4780
Fragments arranged by size:
4780 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5785
Fragments arranged by size:
5785 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2600 1186
Size: 1414 4596
BseYI C'CCAG_C
Cuts at: 5213 5709 5213
Size: 496 5514
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2393 1163
Size: 573 657 4780
Fragments arranged by size:
4780 657 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3460 4221 5249 1540
Size: 275 186 1459 761 1028 2301
Fragments arranged by size:
2301 1459 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2592 3276 4071 4156 5227 2592
Size: 684 795 85 1071 3375
Fragments arranged by size:
3375 1071 795 684 85
BsmI GAATG_Cn'
Cuts at: 2186 2394 2888 3521 3614 2186
Size: 208 494 633 93 4582
Fragments arranged by size:
4582 633 494 208 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2592 3276 3329 4071 4156 5227 1391
Size: 399 802 684 53 742 85 1071 2174
Fragments arranged by size:
2174 1071 802 742 684 399 85 53
BspEI T'CCGG_A
Cuts at: 5673 5673
Size: 6010
BspHI T'CATG_A
Cuts at: 3899 5629 3899
Size: 1730 4280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3365 844
Size: 2521 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3897 1394
Size: 429 2074 3507
Fragments arranged by size:
3507 2074 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2331 4505 4679 65
Size: 1183 1083 2174 174 1396
Fragments arranged by size:
2174 1396 1183 1083 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4654 4841 1700
Size: 110 2844 187 2869
Fragments arranged by size:
2869 2844 187 110
BsrGI T'GTAC_A
Cuts at: 96 2244 96
Size: 2148 3862
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6010
BssSI C'ACGA_G
Cuts at: 4070 5082 4070
Size: 1012 4998
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2129 794
Size: 1335 4675
BstBI TT'CG_AA
Cuts at: 4814 4814
Size: 6010
BstEII G'GTnAC_C
Cuts at: 1184 2492 1184
Size: 1308 4702
BstXI CCAn_nnnn'nTGG
Cuts at: 2732 3155 2732
Size: 423 5587
BstYI r'GATC_y
Cuts at: 1972 2400 2792 3340 3707 4092 4109 5561 5647
Size: 428 392 548 367 385 17 1452 86
Cuts at: 5647 5659 6005 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 548 428 392 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 3111 3111
Size: 6010
Bsu36I CC'TnA_GG
Cuts at: 2762 2762
Size: 6010
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5271
Fragments arranged by size:
5271 683 56
BtsI GCAGTG_nn'
Cuts at: 2010 2130 2541 3526 4320 4340 2010
Size: 120 411 985 794 20 3680
Fragments arranged by size:
3680 985 794 411 120 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6010
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5975
DraI TTT'AAA
Cuts at: 3314 3314
Size: 6010
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2374 5017 809
Size: 507 1058 2643 1802
Fragments arranged by size:
2643 1802 1058 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3457 4346 5975
Size: 54 1425 50 275 18 1627 889 1629
Cuts at: 5975 8
Size: 43
Fragments arranged by size:
1629 1627 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3457 1537
Size: 275 1645 4090
Fragments arranged by size:
4090 1645 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3940 1353
Size: 2587 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4597 4971 5117 387
Size: 1064 138 3008 374 146 1280
Fragments arranged by size:
3008 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3274 3274
Size: 6010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2021 2459 2603 4073 4670 5457 5725
Size: 393 389 438 144 1470 597 787 268
Cuts at: 5725 1239
Size: 1524
Fragments arranged by size:
1524 1470 787 597 438 393 389 268
144
EcoNI CCTnn'n_nnAGG
Cuts at: 3305 3305
Size: 6010
EcoO109I rG'GnC_Cy
Cuts at: 2559 2578 2684 2875 2559
Size: 19 106 191 5694
Fragments arranged by size:
5694 191 106 19
EcoRI G'AATT_C
Cuts at: 3319 3697 3319
Size: 378 5632
FspI TGC'GCA
Cuts at: 4516 4516
Size: 6010
HaeII r_GCGC'y
Cuts at: 1678 5157 1678
Size: 3479 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2260 2292 2427 2459 2469
Size: 32 135 32 688 32 135 32 10
Cuts at: 2469 2501 2548 2580 2621 2653 3185 3217 4651
Size: 32 47 32 41 32 532 32 1434
Cuts at: 4651 4683 4725 4757 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1434 688 532 135 135 47 42
41 32 32 32 32 32 32 32
32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3354 3600 669
Size: 988 281 1416 246 3079
Fragments arranged by size:
3079 1416 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6010
HpaI GTT'AAC
Cuts at: 3600 3600
Size: 6010
KpnI G_GTAC'C
Cuts at: 2952 3335 2952
Size: 383 5627
MfeI C'AATT_G
Cuts at: 2091 3092 3609 2091
Size: 1001 517 4492
Fragments arranged by size:
4492 1001 517
MluI A'CGCG_T
Cuts at: 5886 5886
Size: 6010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2233 3078 5124 5308 1305
Size: 928 845 2046 184 2007
Fragments arranged by size:
2046 2007 928 845 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4188
Fragments arranged by size:
4188 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2730 2781 3968 4327 4486 518
Size: 648 1564 51 1187 359 159 2042
Fragments arranged by size:
2042 1564 1187 648 359 159 51
MspA1I CmG'CkG
Cuts at: 1606 2044 3198 3426 4103 5251 5496 1606
Size: 438 1154 228 677 1148 245 2120
Fragments arranged by size:
2120 1154 1148 677 438 245 228
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4519
Fragments arranged by size:
4519 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6010
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6010
NotI GC'GGCC_GC
Cuts at: 3457 3457
Size: 6010
NsiI A_TGCA'T
Cuts at: 2186 3166 2186
Size: 980 5030
NspI r_CATG'y
Cuts at: 1132 1229 2053 2786 3109 3368 4913 1132
Size: 97 824 733 323 259 1545 2229
Fragments arranged by size:
2229 1545 824 733 323 259 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6010
PciI A'CATG_T
Cuts at: 2782 3105 4909 2782
Size: 323 1804 3883
Fragments arranged by size:
3883 1804 323
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2581 3097 1108
Size: 1473 516 4021
Fragments arranged by size:
4021 1473 516
PmeI GTTT'AAAC
Cuts at: 3314 3314
Size: 6010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4079 4111 5592 5624 4079
Size: 32 1481 32 4465
Fragments arranged by size:
4465 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6010
PsiI TTA'TAA
Cuts at: 3580 3580
Size: 6010
PstI C_TGCA'G
Cuts at: 830 2548 3362 830
Size: 1718 814 3478
Fragments arranged by size:
3478 1718 814
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6010
PvuII CAG'CTG
Cuts at: 2044 3198 2044
Size: 1154 4856
SacI G_AGCT'C
Cuts at: 3276 3276
Size: 6010
SalI G'TCGA_C
Cuts at: 1936 3352 1936
Size: 1416 4594
SbfI CC_TGCA'GG
Cuts at: 3362 3362
Size: 6010
ScaI AGT'ACT
Cuts at: 4258 5970 4258
Size: 1712 4298
SfcI C'TryA_G
Cuts at: 826 1045 2544 3358 4493 5174 5365 826
Size: 219 1499 814 1135 681 191 1471
Fragments arranged by size:
1499 1471 1135 814 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6010
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6010
SmaI CCC'GGG
Cuts at: 1872 3337 1872
Size: 1465 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4114 5015 5277 820
Size: 197 926 2171 901 262 1553
Fragments arranged by size:
2171 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3801 493
Size: 3308 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6010
SphI G_CATG'C
Cuts at: 1132 3368 1132
Size: 2236 3774
SspI AAT'ATT
Cuts at: 5 52 3934 5
Size: 47 3882 2081
Fragments arranged by size:
3882 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6010
StyI C'CwwG_G
Cuts at: 513 1065 2004 2839 3449 513
Size: 552 939 835 610 3074
Fragments arranged by size:
3074 939 835 610 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2588 4037 4054 4207 4392 4837 325
Size: 1255 1008 1449 17 153 185 445 1498
Fragments arranged by size:
1498 1449 1255 1008 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2244 4256 5968
Size: 274 80 33 51 1443 267 2012 1712
Cuts at: 5968 96
Size: 138
Fragments arranged by size:
2012 1712 1443 274 267 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5970 6002 5970
Size: 32 5978
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6010
XbaI T'CTAG_A
Cuts at: 3346 3346
Size: 6010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2578 2578
Size: 6010
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6010
XmaI C'CCGG_G
Cuts at: 1870 3335 1870
Size: 1465 4545
XmnI GAAnn'nnTTC
Cuts at: 2298 4139 2298
Size: 1841 4169
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3818 276
Size: 53 83 186 936 2284 2468
Fragments arranged by size:
2468 2284 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BplI Bpu10I BsiWI BsmBI DraIII EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI