Restriction map for
pFN21AB9481
##################################
# NO CUT ENZYMES ( 46 enzymes) #
##################################
AatII AflII AgeI Aor51HI AscI
AseI AsnI BfrI BglI BsiWI
BspLU11I BssHII BssSI Bst1107I BstBI
BstZ17I CpoI DraIII Eco47III EcoNI
FseI HpaI MfeI MluI MunI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
PvuI RsrII SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Acc65I 4913 GGTACC G^GTACC
AccIII 1060 TCCGGA T^CCGGA
AcyI 2652 GGCGCC G[GA]^CG[CT]C
AflIII 3536 ACACGT A^C[GA][CT]GT
AocI 3924 CCTGAGG CC^T[ACGT]AGG
Asp718I 4913 GGTACC G^GTACC
AvaI 6336 CCCGGG C^[CT]CG[GA]G
AvrII 5316 CCTAGG C^CTAGG
BbeI 2652 GGCGCC GGCGC^C
BbrPI 4491 CACGTG CAC^GTG
BbvCI 2631 CCTCAGC CCTCAGC(-5/-2)
BciVI 2902 GTATCC GTATCC(6/5)
BlnI 5316 CCTAGG C^CTAGG
BsaHI 2652 GGCGCC G[GA]^CG[CT]C
BseAI 1060 TCCGGA T^CCGGA
BsoBI 6336 CCCGGG C^[CT]CG[GA]G
BspEI 1060 TCCGGA T^CCGGA
Bsu36I 3924 CCTGAGG CC^T[ACGT]AGG
DraI 1687 TTTAAA TTT^AAA
EagI 4940 CGGCCG C^GGCCG
EclXI 4940 CGGCCG C^GGCCG
Eco52I 4940 CGGCCG C^GGCCG
Eco81I 3924 CCTGAGG CC^T[ACGT]AGG
EcoRV 3733 GATATC GAT^ATC
EcoT22I 3668 ATGCAT ATGCA^T
HaeII 2652 GGCGCC [GA]GCGC^[CT]
HgaI 4601 GACGC GACGC(5/10)
Hin1I 2652 GGCGCC G[GA]^CG[CT]C
HindIII 5293 AAGCTT A^AGCTT
KasI 2652 GGCGCC G^GCGCC
KpnI 4913 GGTACC GGTAC^C
KspI 225 CCGCGG CCGC^GG
MroI 1060 TCCGGA T^CCGGA
NarI 2652 GGCGCC GG^CGCC
NdeI 1076 CATATG CA^TATG
NsiI 3668 ATGCAT ATGCA^T
PmaCI 4491 CACGTG CAC^GTG
PmlI 4491 CACGTG CAC^GTG
SacII 225 CCGCGG CCGC^GG
SapI 3213 GCTCTTC GCTCTTC(1/4)
ScaI 1138 AGTACT AGT^ACT
SfoI 2652 GGCGCC GGC^GCC
SmaI 6336 CCCGGG CCC^GGG
XcmI 6431 CCAAAGACTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 6336 CCCGGG C^CCGGG
XmaI 6336 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AclI 1223 AACGTT AA^CGTT
6034 AACGTT
AhdI 2355 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6498 GACTGAGTGTC
Alw44I 6358 GTGCAC G^TGCAC
6460 GTGCAC
ApaI 4501 GGGCCC GGGCC^C
6346 GGGCCC
ApaLI 6358 GTGCAC G^TGCAC
6460 GTGCAC
AspEI 2355 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6498 GACTGAGTGTC
AspI 2848 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3165 GACTTGGTC
AviII 3134 TGCGCA TGC^GCA
3767 TGCGCA
BcgI 274 CGATGGACCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
463 GCAGAAGTTTCG
BclI 288 TGATCA T^GATCA
2931 TGATCA
BglII 3813 AGATCT A^GATCT
4395 AGATCT
BlpI 239 GCTCAGC GC^T[ACGT]AGC
3987 GCTCAGC
Bpu1102I 239 GCTCAGC GC^T[ACGT]AGC
3987 GCTCAGC
BsaAI 4366 TACGTG [CT]AC^GT[GA]
4491 CACGTG
BsmBI 2393 GAGACG CGTCTC(1/5)
3452 GAGACG
BspDI 3272 ATCGAT AT^CGAT
5846 ATCGAT
BsrBI 237 CCGCTC CCGCTC(-3/-3)
2833 GAGCGG
BstAPI 3661 GCAGACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4539 GCAGTGGTTGC
BstEII 5677 GGTAACC G^GT[ACGT]ACC
6054 GGTGACC
BstPI 5677 GGTAACC G^GT[ACGT]ACC
6054 GGTGACC
CelII 239 GCTCAGC GC^T[ACGT]AGC
3987 GCTCAGC
ClaI 3272 ATCGAT AT^CGAT
5846 ATCGAT
DrdI 4051 GACCCACTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6262 GACCATGGAGTC
Eam1105I 2355 GACCAGTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
6498 GACTGAGTGTC
EcoO65I 5677 GGTAACC G^GT[ACGT]ACC
6054 GGTGACC
FbaI 288 TGATCA T^GATCA
2931 TGATCA
FspI 3134 TGCGCA TGC^GCA
3767 TGCGCA
NaeI 4742 GCCGGC GCC^GGC
4942 GCCGGC
NgoMIV 4742 GCCGGC G^CCGGC
4942 GCCGGC
PflFI 2848 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3165 GACTTGGTC
Psp1406I 1223 AACGTT AA^CGTT
6034 AACGTT
SacI 3985 GAGCTC GAGCT^C
4040 GAGCTC
SexAI 2225 ACCTGGT A^CC[AT]GGT
4916 ACCTGGT
SpeI 1503 ACTAGT A^CTAGT
4056 ACTAGT
SphI 2913 GCATGC GCATG^C
5546 GCATGC
SspI 1192 AATATT AAT^ATT
3388 AATATT
Tth111I 2848 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3165 GACTTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BamHI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaBI BsaI
BsaJI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BspHI BspMI
BsrDI BsrFI BsrGI BsrI BssKI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MamI MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI SalI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII XmnI