(Circular) (Six-base) MAPSORT of: pFN21AB8506.seq Check: 1848 from: 1 to: 7303 pFN21AB8506 7303 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5113 278 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2856 4646 1937 Size: 919 1790 4594 Fragments arranged by size: 4594 1790 919 Acc65I G'GTAC_C Cuts at: 4624 4624 Size: 7303 AclI AA'CG_TT Cuts at: 1563 4602 5430 5803 1563 Size: 3039 828 373 3063 Fragments arranged by size: 3063 3039 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2600 2675 2685 3041 3126 3219 5366 6750 Size: 968 75 10 356 85 93 2147 1384 Cuts at: 6750 7018 1632 Size: 268 1917 Fragments arranged by size: 2147 1917 1384 968 356 268 93 85 75 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7106 AflIII A'CryG_T Cuts at: 1203 2125 2135 6202 7179 1203 Size: 922 10 4067 977 1327 Fragments arranged by size: 4067 1327 977 922 10 AgeI A'CCGG_T Cuts at: 6134 6134 Size: 7303 AhdI GACnn_n'nnGTC Cuts at: 2863 6032 2863 Size: 3169 4134 AleI CACnn'nnGTG Cuts at: 1166 3227 3711 1166 Size: 2061 484 4758 Fragments arranged by size: 4758 2061 484 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2043 1184 Size: 859 6444 AlwNI CAG_nnn'CTG Cuts at: 2195 2416 3466 4483 5046 6618 2195 Size: 221 1050 1017 563 1572 2880 Fragments arranged by size: 2880 1572 1050 1017 563 221 ApaI G_GGCC'C Cuts at: 4538 4538 Size: 7303 ApaLI G'TGCA_C Cuts at: 5360 6516 5360 Size: 1156 6147 ApoI r'AATT_y Cuts at: 1493 3152 3797 4612 4838 4990 7099 1493 Size: 1659 645 815 226 152 2109 1697 Fragments arranged by size: 2109 1697 1659 815 645 226 152 AseI AT'TA_AT Cuts at: 160 1032 3492 5857 160 Size: 872 2460 2365 1606 Fragments arranged by size: 2460 2365 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 AvaI C'yCGr_G Cuts at: 1870 1943 3173 3430 4628 1870 Size: 73 1230 257 1198 4545 Fragments arranged by size: 4545 1230 1198 257 73 BamHI G'GATC_C Cuts at: 2498 2641 4633 5000 2498 Size: 143 1992 367 4801 Fragments arranged by size: 4801 1992 367 143 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4624 6079 618 Size: 325 217 627 2837 1455 1842 Fragments arranged by size: 2837 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3681 4082 4538 4622 1391 Size: 2290 401 456 84 4072 Fragments arranged by size: 4072 2290 456 401 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2354 928 Size: 971 455 5877 Fragments arranged by size: 5877 971 455 BbvCI CC'TCA_GC Cuts at: 3353 3353 Size: 7303 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5494 5528 1903 Size: 34 3557 34 3678 Fragments arranged by size: 3678 3557 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5195 6405 5195 Size: 1210 6093 BclI T'GATC_A Cuts at: 1548 1548 Size: 7303 BfrBI ATG'CAT Cuts at: 4518 4518 Size: 7303 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5914 136 Size: 107 122 71 631 605 4242 1525 Fragments arranged by size: 4242 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2482 7298 2482 Size: 4816 2487 BlpI GC'TnA_GC Cuts at: 3573 4720 3573 Size: 1147 6156 Bme1580I G_kGCm'C Cuts at: 1790 3985 4538 5364 6520 1790 Size: 2195 553 826 1156 2573 Fragments arranged by size: 2573 2195 1156 826 553 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2215 2401 3085 Size: 416 53 83 399 24 798 186 684 Cuts at: 3085 5034 5992 442 Size: 1949 958 1753 Fragments arranged by size: 1949 1753 958 798 684 416 399 186 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3487 3644 3487 Size: 157 7146 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2478 3033 3236 3278 3665 4242 5963 Size: 953 286 555 203 42 387 577 1721 Cuts at: 5963 1239 Size: 2579 Fragments arranged by size: 2579 1721 953 577 555 387 286 203 42 Bpu10I CC'TnA_GC Cuts at: 3353 3353 Size: 7303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5428 6293 6591 5428 Size: 865 298 6140 Fragments arranged by size: 6140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2192 2871 5966 882 Size: 617 693 679 3095 2219 Fragments arranged by size: 3095 2219 693 679 617 BsaAI yAC'GTr Cuts at: 493 1204 5094 493 Size: 711 3890 2702 Fragments arranged by size: 3890 2702 711 BsaBI GATnn'nnATC Cuts at: 7297 7297 Size: 7303 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5110 5492 6127 275 Size: 53 83 186 936 3577 382 635 1451 Fragments arranged by size: 3577 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5736 6134 6408 6555 6966 5736 Size: 398 274 147 411 6073 Fragments arranged by size: 6073 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7078 Fragments arranged by size: 7078 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3351 4018 4039 4207 1186 Size: 2165 667 21 168 4282 Fragments arranged by size: 4282 2165 667 168 21 BseYI C'CCAG_C Cuts at: 2067 3659 4072 6506 7002 2067 Size: 1592 413 2434 496 2368 Fragments arranged by size: 2434 2368 1592 496 413 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3029 1163 Size: 573 1293 5437 Fragments arranged by size: 5437 1293 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4753 5514 6542 1540 Size: 275 186 2752 761 1028 2301 Fragments arranged by size: 2752 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3373 3681 4438 5364 5449 6520 3373 Size: 308 757 926 85 1071 4156 Fragments arranged by size: 4156 1071 926 757 308 85 BsmI GAATG_Cn' Cuts at: 2817 3833 4520 4814 4907 2817 Size: 1016 687 294 93 5213 Fragments arranged by size: 5213 1016 687 294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3373 3681 3985 4082 4438 4538 4622 Size: 399 1583 308 304 97 356 100 84 Cuts at: 4622 5364 5449 6520 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1583 1071 742 399 356 308 304 100 97 85 84 BspEI T'CCGG_A Cuts at: 6966 6966 Size: 7303 BspHI T'CATG_A Cuts at: 5192 6922 5192 Size: 1730 5573 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4658 844 Size: 3814 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5190 1394 Size: 429 3367 3507 Fragments arranged by size: 3507 3367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2487 5798 5972 65 Size: 1183 1239 3311 174 1396 Fragments arranged by size: 3311 1396 1239 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 3804 5947 6134 1700 Size: 110 226 1768 2143 187 2869 Fragments arranged by size: 2869 2143 1768 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7303 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7303 BssSI C'ACGA_G Cuts at: 3372 3443 5363 6375 3372 Size: 71 1920 1012 4300 Fragments arranged by size: 4300 1920 1012 71 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7303 BstBI TT'CG_AA Cuts at: 6107 6107 Size: 7303 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7303 BstXI CCAn_nnnn'nTGG Cuts at: 4094 4094 Size: 7303 BstYI r'GATC_y Cuts at: 1972 2191 2482 2498 2641 2877 4421 4633 5000 Size: 219 291 16 143 236 1544 212 367 Cuts at: 5000 5385 5402 6854 6940 6952 7298 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1544 1452 385 367 346 291 236 219 212 143 86 17 16 12 Bsu36I CC'TnA_GG Cuts at: 3452 3452 Size: 7303 BtgI C'CryG_G Cuts at: 513 1065 3306 3851 513 Size: 552 2241 545 3965 Fragments arranged by size: 3965 2241 552 545 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4233 487 Size: 683 56 3007 3557 Fragments arranged by size: 3557 3007 683 56 BtsI GCAGTG_nn' Cuts at: 3017 3222 3234 3405 4819 5613 5633 3017 Size: 205 12 171 1414 794 20 4687 Fragments arranged by size: 4687 1414 794 205 171 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3079 3114 529 Size: 35 2515 35 4718 Fragments arranged by size: 4718 2515 35 35 DraI TTT'AAA Cuts at: 2122 4607 2122 Size: 2485 4818 DraIII CAC_nnn'GTG Cuts at: 3959 3959 Size: 7303 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2511 6310 809 Size: 507 1195 3799 1802 Fragments arranged by size: 3799 1802 1195 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2748 3343 4750 Size: 54 1425 50 275 18 918 595 1407 Cuts at: 4750 5639 7268 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1407 918 889 595 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4750 1537 Size: 275 2938 4090 Fragments arranged by size: 4090 2938 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 2760 2766 3848 4225 5233 1353 Size: 693 714 6 1082 377 1008 3423 Fragments arranged by size: 3423 1082 1008 714 693 377 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4030 5890 6264 6410 387 Size: 1064 138 2441 1860 374 146 1280 Fragments arranged by size: 2441 1860 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3679 3679 Size: 7303 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2478 2600 2675 2685 3033 3041 Size: 393 560 286 122 75 10 348 8 Cuts at: 3041 3126 3219 3236 3278 3665 4242 5366 5963 Size: 85 93 17 42 387 577 1124 597 Cuts at: 5963 6750 7018 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1124 787 597 577 560 393 387 348 286 268 122 93 85 75 42 17 10 8 EcoNI CCTnn'n_nnAGG Cuts at: 3620 4460 3620 Size: 840 6463 EcoO109I rG'GnC_Cy Cuts at: 2455 3448 3593 3625 4480 4535 2455 Size: 993 145 32 855 55 5223 Fragments arranged by size: 5223 993 855 145 55 32 EcoRI G'AATT_C Cuts at: 3797 4612 4990 3797 Size: 815 378 6110 Fragments arranged by size: 6110 815 378 EcoRV GAT'ATC Cuts at: 4242 4242 Size: 7303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3538 3538 Size: 7303 FspI TGC'GCA Cuts at: 5809 5809 Size: 7303 HaeII r_GCGC'y Cuts at: 1678 6450 1678 Size: 4772 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2024 2056 2176 2208 2369 Size: 32 135 32 452 32 120 32 161 Cuts at: 2369 2401 2849 2881 3680 3712 5944 5976 6018 Size: 32 448 32 799 32 2232 32 42 Cuts at: 6018 6050 1373 Size: 32 2626 Fragments arranged by size: 2626 2232 799 452 448 161 135 120 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4647 4893 669 Size: 988 281 2709 246 3079 Fragments arranged by size: 3079 2709 988 281 246 HindIII A'AGCT_T Cuts at: 748 2517 748 Size: 1769 5534 HpaI GTT'AAC Cuts at: 4893 4893 Size: 7303 KpnI G_GTAC'C Cuts at: 4628 4628 Size: 7303 MfeI C'AATT_G Cuts at: 4902 4902 Size: 7303 MluI A'CGCG_T Cuts at: 7179 7179 Size: 7303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6417 6601 1305 Size: 5112 184 2007 Fragments arranged by size: 5112 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2750 10 Size: 54 1425 343 918 4563 Fragments arranged by size: 4563 1425 918 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3227 3711 5261 5620 5779 518 Size: 648 2061 484 1550 359 159 2042 Fragments arranged by size: 2061 2042 1550 648 484 359 159 MspA1I CmG'CkG Cuts at: 1606 2446 3256 3685 4076 4719 5396 6544 6789 Size: 840 810 429 391 643 677 1148 245 Cuts at: 6789 1606 Size: 2120 Fragments arranged by size: 2120 1148 840 810 677 643 429 391 245 NaeI GCC'GGC Cuts at: 2038 3806 2038 Size: 1768 5535 NcoI C'CATG_G Cuts at: 513 1065 3306 513 Size: 552 2241 4510 Fragments arranged by size: 4510 2241 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7303 NgoMIV G'CCGG_C Cuts at: 2036 3804 2036 Size: 1768 5535 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7303 NotI GC'GGCC_GC Cuts at: 4750 4750 Size: 7303 NsiI A_TGCA'T Cuts at: 4520 4520 Size: 7303 NspI r_CATG'y Cuts at: 1132 1229 2129 2430 4661 6206 1132 Size: 97 900 301 2231 1545 2229 Fragments arranged by size: 2231 2229 1545 900 301 97 PasI CC'CwG_GG Cuts at: 1348 4087 1348 Size: 2739 4564 PciI A'CATG_T Cuts at: 2125 6202 2125 Size: 4077 3226 PflMI CCAn_nnn'nTGG Cuts at: 1108 2328 3959 1108 Size: 1220 1631 4452 Fragments arranged by size: 4452 1631 1220 PfoI T'CCnGG_A Cuts at: 2494 2523 2494 Size: 29 7274 PmeI GTTT'AAAC Cuts at: 4607 4607 Size: 7303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5372 5404 6885 6917 5372 Size: 32 1481 32 5758 Fragments arranged by size: 5758 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3593 3625 3593 Size: 32 7271 PshAI GACnn'nnGTC Cuts at: 1613 2943 1613 Size: 1330 5973 PsiI TTA'TAA Cuts at: 4873 4873 Size: 7303 PspOMI G'GGCC_C Cuts at: 4534 4534 Size: 7303 PspXI vC'TCGA_Gb Cuts at: 3173 3430 3173 Size: 257 7046 PstI C_TGCA'G Cuts at: 830 3229 4655 830 Size: 2399 1426 3478 Fragments arranged by size: 3478 2399 1426 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7303 PvuII CAG'CTG Cuts at: 3256 3685 3256 Size: 429 6874 SacI G_AGCT'C Cuts at: 3681 3681 Size: 7303 SalI G'TCGA_C Cuts at: 1936 4645 1936 Size: 2709 4594 SapI GCTCTTCn'nnn_ Cuts at: 2766 2766 Size: 7303 SbfI CC_TGCA'GG Cuts at: 4655 4655 Size: 7303 ScaI AGT'ACT Cuts at: 2918 5551 7263 2918 Size: 2633 1712 2958 Fragments arranged by size: 2958 2633 1712 SexAI A'CCwGG_T Cuts at: 3955 3955 Size: 7303 SfcI C'TryA_G Cuts at: 826 1045 2553 2853 2889 3225 4178 4651 5786 Size: 219 1508 300 36 336 953 473 1135 Cuts at: 5786 6467 6658 826 Size: 681 191 1471 Fragments arranged by size: 1508 1471 1135 953 681 473 336 300 219 191 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7303 SmaI CCC'GGG Cuts at: 1872 4630 1872 Size: 2758 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3173 3430 5407 6308 6570 820 Size: 197 926 1230 257 1977 901 262 1553 Fragments arranged by size: 1977 1553 1230 926 901 262 257 197 SnaBI TAC'GTA Cuts at: 493 5094 493 Size: 4601 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7303 SphI G_CATG'C Cuts at: 1132 4661 1132 Size: 3529 3774 SspI AAT'ATT Cuts at: 5 52 3503 4452 5227 5 Size: 47 3451 949 775 2081 Fragments arranged by size: 3451 2081 949 775 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7303 StyI C'CwwG_G Cuts at: 513 1065 3306 4742 513 Size: 552 2241 1436 3074 Fragments arranged by size: 3074 2241 1436 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2316 3836 4244 5330 5347 5500 5685 Size: 1255 736 1520 408 1086 17 153 185 Cuts at: 5685 6130 325 Size: 445 1498 Fragments arranged by size: 1520 1498 1255 1086 736 445 408 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2916 3889 4154 Size: 274 80 33 51 1443 939 973 265 Cuts at: 4154 5549 7261 96 Size: 1395 1712 138 Fragments arranged by size: 1712 1443 1395 973 939 274 265 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2089 2121 7263 7295 2089 Size: 32 5142 32 2097 Fragments arranged by size: 5142 2097 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7303 XbaI T'CTAG_A Cuts at: 2080 4639 2080 Size: 2559 4744 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4469 4469 Size: 7303 XhoI C'TCGA_G Cuts at: 1943 3173 3430 1943 Size: 1230 257 5816 Fragments arranged by size: 5816 1230 257 XmaI C'CCGG_G Cuts at: 1870 4628 1870 Size: 2758 4545 XmnI GAAnn'nnTTC Cuts at: 4362 5432 4362 Size: 1070 6233 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5111 276 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstZ17I FseI FspAI KasI NarI NruI PacI PmlI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI