(Circular) (Six-base) MAPSORT of: pFN21AE0029.seq Check: 1867 from: 1 to: 6295 pFN21AE0029 6295 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4105 278 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3638 1937 Size: 1701 4594 Acc65I G'GTAC_C Cuts at: 3616 3616 Size: 6295 AclI AA'CG_TT Cuts at: 1563 4422 4795 1563 Size: 2859 373 3063 Fragments arranged by size: 3063 2859 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4358 5742 6010 1632 Size: 2726 1384 268 1917 Fragments arranged by size: 2726 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2216 3127 3331 820 Size: 197 1199 911 204 3784 Fragments arranged by size: 3784 1199 911 204 197 AflIII A'CryG_T Cuts at: 1203 5194 6171 1203 Size: 3991 977 1327 Fragments arranged by size: 3991 1327 977 AgeI A'CCGG_T Cuts at: 2532 5126 2532 Size: 2594 3701 AhdI GACnn_n'nnGTC Cuts at: 5024 5024 Size: 6295 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6295 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6295 AlwNI CAG_nnn'CTG Cuts at: 3176 4038 5610 3176 Size: 862 1572 3861 Fragments arranged by size: 3861 1572 862 ApaLI G'TGCA_C Cuts at: 4352 5508 4352 Size: 1156 5139 ApoI r'AATT_y Cuts at: 1493 2048 2325 2708 3604 3830 3982 6091 1493 Size: 555 277 383 896 226 152 2109 1697 Fragments arranged by size: 2109 1697 896 555 383 277 226 152 AseI AT'TA_AT Cuts at: 160 1032 4849 160 Size: 872 3817 1606 Fragments arranged by size: 3817 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 AvaI C'yCGr_G Cuts at: 1870 1943 3201 3620 1870 Size: 73 1258 419 4545 Fragments arranged by size: 4545 1258 419 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2100 2133 2100 Size: 33 6262 BamHI G'GATC_C Cuts at: 2432 2701 3625 3992 2432 Size: 269 924 367 4735 Fragments arranged by size: 4735 924 367 269 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3313 3616 5071 618 Size: 325 217 627 1526 303 1455 1842 Fragments arranged by size: 1842 1526 1455 627 325 303 217 BanII G_rGCy'C Cuts at: 1391 2160 2193 2806 3200 3522 3538 3614 1391 Size: 769 33 613 394 322 16 76 4072 Fragments arranged by size: 4072 769 613 394 322 76 33 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3492 928 Size: 971 1593 3731 Fragments arranged by size: 3731 1593 971 BbvCI CC'TCA_GC Cuts at: 3000 3074 3524 3000 Size: 74 450 5771 Fragments arranged by size: 5771 450 74 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4486 4520 1903 Size: 34 50 34 2465 34 3678 Fragments arranged by size: 3678 2465 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4187 5397 4187 Size: 1210 5085 BclI T'GATC_A Cuts at: 1548 1548 Size: 6295 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4906 136 Size: 107 122 71 631 605 3234 1525 Fragments arranged by size: 3234 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6290 6290 Size: 6295 BlpI GC'TnA_GC Cuts at: 3712 3712 Size: 6295 Bme1580I G_kGCm'C Cuts at: 1790 4356 5512 1790 Size: 2566 1156 2573 Fragments arranged by size: 2573 2566 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6295 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3153 4026 4984 Size: 416 53 83 399 24 1736 873 958 Cuts at: 4984 442 Size: 1753 Fragments arranged by size: 1753 1736 958 873 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6295 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2958 2958 Size: 6295 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 4955 1239 Size: 944 2772 2579 Fragments arranged by size: 2772 2579 944 Bpu10I CC'TnA_GC Cuts at: 3000 3074 3290 3524 3000 Size: 74 216 234 5771 Fragments arranged by size: 5771 234 216 74 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3346 4420 5285 5583 3346 Size: 1074 865 298 4058 Fragments arranged by size: 4058 1074 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4958 882 Size: 617 3459 2219 Fragments arranged by size: 3459 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4086 493 Size: 711 2882 2702 Fragments arranged by size: 2882 2702 711 BsaBI GATnn'nnATC Cuts at: 2499 3058 6289 2499 Size: 559 3231 2505 Fragments arranged by size: 3231 2505 559 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4102 4484 5119 275 Size: 53 83 186 936 2569 382 635 1451 Fragments arranged by size: 2569 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2100 2532 3342 4728 5126 5400 5547 5958 2100 Size: 432 810 1386 398 274 147 411 2437 Fragments arranged by size: 2437 1386 810 432 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6070 Fragments arranged by size: 6070 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3030 3035 3097 3512 1186 Size: 1844 5 62 415 3969 Fragments arranged by size: 3969 1844 415 62 5 BseYI C'CCAG_C Cuts at: 2008 2805 5498 5994 2008 Size: 797 2693 496 2309 Fragments arranged by size: 2693 2309 797 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 1163 Size: 573 772 4950 Fragments arranged by size: 4950 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3745 4506 5534 1540 Size: 275 186 1744 761 1028 2301 Fragments arranged by size: 2301 1744 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2193 3522 4356 4441 5512 2160 Size: 33 1329 834 85 1071 2943 Fragments arranged by size: 2943 1329 1071 834 85 33 BsmI GAATG_Cn' Cuts at: 3806 3899 3806 Size: 93 6202 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2193 2806 3200 3522 3538 3614 Size: 399 370 33 613 394 322 16 76 Cuts at: 3614 4356 4441 5512 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 613 399 394 370 322 85 76 33 16 BspEI T'CCGG_A Cuts at: 3342 5958 3342 Size: 2616 3679 BspHI T'CATG_A Cuts at: 4184 5914 4184 Size: 1730 4565 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3650 844 Size: 2806 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4182 1394 Size: 429 2359 3507 Fragments arranged by size: 3507 2359 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4790 4964 65 Size: 1183 3542 174 1396 Fragments arranged by size: 3542 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2532 3489 4939 5126 1700 Size: 110 722 957 1450 187 2869 Fragments arranged by size: 2869 1450 957 722 187 110 BsrGI T'GTAC_A Cuts at: 96 3229 96 Size: 3133 3162 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6295 BssSI C'ACGA_G Cuts at: 4355 5367 4355 Size: 1012 5283 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 794 Size: 1445 4850 BstBI TT'CG_AA Cuts at: 5099 5099 Size: 6295 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6295 BstYI r'GATC_y Cuts at: 1972 2432 2701 3625 3992 4377 4394 5846 5932 Size: 460 269 924 367 385 17 1452 86 Cuts at: 5932 5944 6290 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 924 460 385 367 346 269 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2436 2651 513 Size: 552 1371 215 4157 Fragments arranged by size: 4157 1371 552 215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5556 Fragments arranged by size: 5556 683 56 BtsI GCAGTG_nn' Cuts at: 2228 2520 3811 4605 4625 2228 Size: 292 1291 794 20 3898 Fragments arranged by size: 3898 1291 794 292 20 ClaI AT'CG_AT Cuts at: 1554 3460 1554 Size: 1906 4389 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6260 DraI TTT'AAA Cuts at: 3599 3599 Size: 6295 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3550 5302 809 Size: 507 2234 1752 1802 Fragments arranged by size: 2234 1802 1752 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2770 3304 3742 Size: 54 1425 50 275 18 940 534 438 Cuts at: 3742 4631 6260 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 940 889 534 438 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3742 1537 Size: 275 1930 4090 Fragments arranged by size: 4090 1930 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2983 3115 4225 1353 Size: 1630 132 1110 3423 Fragments arranged by size: 3423 1630 1110 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4882 5256 5402 387 Size: 1064 138 3293 374 146 1280 Fragments arranged by size: 3293 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2158 2191 3520 2158 Size: 33 1329 4933 Fragments arranged by size: 4933 1329 33 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 4358 4955 5742 6010 1239 Size: 393 551 2175 597 787 268 1524 Fragments arranged by size: 2175 1524 787 597 551 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2771 2771 Size: 6295 EcoO109I rG'GnC_Cy Cuts at: 2764 2764 Size: 6295 EcoRI G'AATT_C Cuts at: 2048 3604 3982 2048 Size: 1556 378 4361 Fragments arranged by size: 4361 1556 378 EcoRV GAT'ATC Cuts at: 2791 2791 Size: 6295 FspI TGC'GCA Cuts at: 4801 4801 Size: 6295 HaeII r_GCGC'y Cuts at: 1678 5442 1678 Size: 3764 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4936 4968 5010 5042 1373 Size: 32 135 32 3364 32 42 32 2626 Fragments arranged by size: 3364 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3639 3885 669 Size: 988 281 1701 246 3079 Fragments arranged by size: 3079 1701 988 281 246 HindIII A'AGCT_T Cuts at: 748 3124 748 Size: 2376 3919 HpaI GTT'AAC Cuts at: 3885 3885 Size: 6295 KpnI G_GTAC'C Cuts at: 3620 3620 Size: 6295 MfeI C'AATT_G Cuts at: 2427 3087 3894 2427 Size: 660 807 4828 Fragments arranged by size: 4828 807 660 MluI A'CGCG_T Cuts at: 6171 6171 Size: 6295 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2597 2948 5409 5593 1305 Size: 1292 351 2461 184 2007 Fragments arranged by size: 2461 2007 1292 351 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2772 3306 10 Size: 54 1425 343 940 534 2999 Fragments arranged by size: 2999 1425 940 534 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4253 4612 4771 518 Size: 648 3087 359 159 2042 Fragments arranged by size: 3087 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2182 3711 4388 5536 5781 1606 Size: 576 1529 677 1148 245 2120 Fragments arranged by size: 2120 1529 1148 677 576 245 NcoI C'CATG_G Cuts at: 513 1065 2436 513 Size: 552 1371 4372 Fragments arranged by size: 4372 1371 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6295 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6295 NotI GC'GGCC_GC Cuts at: 3742 3742 Size: 6295 NspI r_CATG'y Cuts at: 1132 1229 3653 5198 1132 Size: 97 2424 1545 2229 Fragments arranged by size: 2424 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6295 PciI A'CATG_T Cuts at: 5194 5194 Size: 6295 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6295 PfoI T'CCnGG_A Cuts at: 2160 2160 Size: 6295 PmeI GTTT'AAAC Cuts at: 3599 3599 Size: 6295 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4364 4396 5877 5909 4364 Size: 32 1481 32 4750 Fragments arranged by size: 4750 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2764 2764 Size: 6295 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6295 PsiI TTA'TAA Cuts at: 2568 3865 2568 Size: 1297 4998 PstI C_TGCA'G Cuts at: 830 2235 3483 3647 830 Size: 1405 1248 164 3478 Fragments arranged by size: 3478 1405 1248 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6295 PvuII CAG'CTG Cuts at: 2182 2182 Size: 6295 SacI G_AGCT'C Cuts at: 2160 2193 3522 2160 Size: 33 1329 4933 Fragments arranged by size: 4933 1329 33 SalI G'TCGA_C Cuts at: 1936 3637 1936 Size: 1701 4594 SbfI CC_TGCA'GG Cuts at: 3647 3647 Size: 6295 ScaI AGT'ACT Cuts at: 4543 6255 4543 Size: 1712 4583 SfcI C'TryA_G Cuts at: 826 1045 2231 3479 3643 4778 5459 5650 826 Size: 219 1186 1248 164 1135 681 191 1471 Fragments arranged by size: 1471 1248 1186 1135 681 219 191 164 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6295 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6295 SmaI CCC'GGG Cuts at: 1872 3622 1872 Size: 1750 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2216 3127 3201 3331 3361 4399 Size: 197 926 273 911 74 130 30 1038 Cuts at: 4399 5300 5562 820 Size: 901 262 1553 Fragments arranged by size: 1553 1038 926 911 901 273 262 197 130 74 30 SnaBI TAC'GTA Cuts at: 493 4086 493 Size: 3593 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6295 SphI G_CATG'C Cuts at: 1132 3653 1132 Size: 2521 3774 SspI AAT'ATT Cuts at: 5 52 4219 5 Size: 47 4167 2081 Fragments arranged by size: 4167 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6295 StyI C'CwwG_G Cuts at: 513 1065 2436 2767 3156 3734 513 Size: 552 1371 331 389 578 3074 Fragments arranged by size: 3074 1371 578 552 389 331 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4322 4339 4492 4677 5122 325 Size: 1255 2742 17 153 185 445 1498 Fragments arranged by size: 2742 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3229 4541 6253 Size: 274 80 33 51 1443 1252 1312 1712 Cuts at: 6253 96 Size: 138 Fragments arranged by size: 1712 1443 1312 1252 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3337 3369 6255 6287 3337 Size: 32 2886 32 3345 Fragments arranged by size: 3345 2886 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6295 XbaI T'CTAG_A Cuts at: 3631 3631 Size: 6295 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2250 2250 Size: 6295 XhoI C'TCGA_G Cuts at: 1943 3201 1943 Size: 1258 5037 XmaI C'CCGG_G Cuts at: 1870 3620 1870 Size: 1750 4545 XmnI GAAnn'nnTTC Cuts at: 2313 4424 2313 Size: 2111 4184 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4103 276 Size: 53 83 186 936 2569 2468 Fragments arranged by size: 2569 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI