Restriction map for
pFN21AE0029
(Linear) (Six-base) MAPSORT of: KIEE0029.seq Check: 1571 from: 1 to: 1590
KIEE0029.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AflII C'TTAA_G
Cuts at: 0 211 1122 1326 1590
Size: 211 911 204 264
Fragments arranged by size:
911 264 211 204
AgeI A'CCGG_T
Cuts at: 0 527 1590
Size: 527 1063
AlwNI CAG_nnn'CTG
Cuts at: 0 1171 1590
Size: 1171 419
ApoI r'AATT_y
Cuts at: 0 43 320 703 1590
Size: 43 277 383 887
Fragments arranged by size:
887 383 277 43
AvaI C'yCGr_G
Cuts at: 0 1196 1590
Size: 1196 394
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 95 128 1590
Size: 95 33 1462
Fragments arranged by size:
1462 95 33
BamHI G'GATC_C
Cuts at: 0 427 696 1590
Size: 427 269 894
Fragments arranged by size:
894 427 269
BanI G'GyrC_C
Cuts at: 0 1308 1590
Size: 1308 282
BanII G_rGCy'C
Cuts at: 0 155 188 801 1195 1517 1533 1590
Size: 155 33 613 394 322 16 57
Fragments arranged by size:
613 394 322 155 57 33 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 1487 1590
Size: 1487 103
BbvCI CC'TCA_GC
Cuts at: 0 995 1069 1519 1590
Size: 995 74 450 71
Fragments arranged by size:
995 450 74 71
BmrI ACTGGGnnnn_n'
Cuts at: 0 1148 1590
Size: 1148 442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 953 1590
Size: 953 637
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1590
Size: 178 1412
Bpu10I CC'TnA_GC
Cuts at: 0 995 1069 1285 1519 1590
Size: 995 74 216 234 71
Fragments arranged by size:
995 234 216 74 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1341 1590
Size: 1341 249
BsaBI GATnn'nnATC
Cuts at: 0 494 1053 1590
Size: 494 559 537
Fragments arranged by size:
559 537 494
BsaWI w'CCGG_w
Cuts at: 0 95 527 1337 1590
Size: 95 432 810 253
Fragments arranged by size:
810 432 253 95
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1025 1030 1092 1507 1590
Size: 1025 5 62 415 83
Fragments arranged by size:
1025 415 83 62 5
BseYI C'CCAG_C
Cuts at: 0 3 800 1590
Size: 3 797 790
Fragments arranged by size:
797 790 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1590
Size: 503 1087
BsiHKAI G_wGCw'C
Cuts at: 0 155 188 1517 1590
Size: 155 33 1329 73
Fragments arranged by size:
1329 155 73 33
Bsp1286I G_dGCh'C
Cuts at: 0 155 188 801 1195 1517 1533 1590
Size: 155 33 613 394 322 16 57
Fragments arranged by size:
613 394 322 155 57 33 16
BspEI T'CCGG_A
Cuts at: 0 1337 1590
Size: 1337 253
BsrFI r'CCGG_y
Cuts at: 0 527 1484 1590
Size: 527 957 106
Fragments arranged by size:
957 527 106
BsrGI T'GTAC_A
Cuts at: 0 1224 1590
Size: 1224 366
BstAPI GCAn_nnn'nTGC
Cuts at: 0 234 1590
Size: 234 1356
BstYI r'GATC_y
Cuts at: 0 427 696 1590
Size: 427 269 894
Fragments arranged by size:
894 427 269
BtgI C'CryG_G
Cuts at: 0 431 646 1590
Size: 431 215 944
Fragments arranged by size:
944 431 215
BtsI GCAGTG_nn'
Cuts at: 0 223 515 1590
Size: 223 292 1075
Fragments arranged by size:
1075 292 223
ClaI AT'CG_AT
Cuts at: 0 1455 1590
Size: 1455 135
DrdI GACnn_nn'nnGTC
Cuts at: 0 1545 1590
Size: 1545 45
EaeI y'GGCC_r
Cuts at: 0 765 1299 1590
Size: 765 534 291
Fragments arranged by size:
765 534 291
EarI CTCTTCn'nnn_
Cuts at: 0 978 1110 1590
Size: 978 132 480
Fragments arranged by size:
978 480 132
EcoICRI GAG'CTC
Cuts at: 0 153 186 1515 1590
Size: 153 33 1329 75
Fragments arranged by size:
1329 153 75 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1590
Size: 178 1412
EcoNI CCTnn'n_nnAGG
Cuts at: 0 766 1590
Size: 766 824
EcoO109I rG'GnC_Cy
Cuts at: 0 759 1590
Size: 759 831
EcoRI G'AATT_C
Cuts at: 0 43 1590
Size: 43 1547
EcoRV GAT'ATC
Cuts at: 0 786 1590
Size: 786 804
HindIII A'AGCT_T
Cuts at: 0 1119 1590
Size: 1119 471
MfeI C'AATT_G
Cuts at: 0 422 1082 1590
Size: 422 660 508
Fragments arranged by size:
660 508 422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 943 1590
Size: 592 351 647
Fragments arranged by size:
647 592 351
MscI TGG'CCA
Cuts at: 0 767 1301 1590
Size: 767 534 289
Fragments arranged by size:
767 534 289
MspA1I CmG'CkG
Cuts at: 0 177 1590
Size: 177 1413
NcoI C'CATG_G
Cuts at: 0 431 1590
Size: 431 1159
PfoI T'CCnGG_A
Cuts at: 0 155 1590
Size: 155 1435
PpuMI rG'GwC_Cy
Cuts at: 0 759 1590
Size: 759 831
PsiI TTA'TAA
Cuts at: 0 563 1590
Size: 563 1027
PstI C_TGCA'G
Cuts at: 0 230 1478 1590
Size: 230 1248 112
Fragments arranged by size:
1248 230 112
PvuII CAG'CTG
Cuts at: 0 177 1590
Size: 177 1413
SacI G_AGCT'C
Cuts at: 0 155 188 1517 1590
Size: 155 33 1329 73
Fragments arranged by size:
1329 155 73 33
SfcI C'TryA_G
Cuts at: 0 226 1474 1590
Size: 226 1248 116
Fragments arranged by size:
1248 226 116
SmlI C'TyrA_G
Cuts at: 0 211 1122 1196 1326 1356 1590
Size: 211 911 74 130 30 234
Fragments arranged by size:
911 234 211 130 74 30
StyI C'CwwG_G
Cuts at: 0 431 762 1151 1590
Size: 431 331 389 439
Fragments arranged by size:
439 431 389 331
TatI w'GTAC_w
Cuts at: 0 1224 1590
Size: 1224 366
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1332 1364 1590
Size: 1332 32 226
Fragments arranged by size:
1332 226 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 245 1590
Size: 245 1345
XhoI C'TCGA_G
Cuts at: 0 1196 1590
Size: 1196 394
XmnI GAAnn'nnTTC
Cuts at: 0 308 1590
Size: 308 1282
Enzymes that do cut:
AflII AgeI AlwNI ApoI AvaI BaeI BamHI BanI
BanII BbsI BbvCI BmrI BplI BpmI Bpu10I BpuEI
BsaBI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI
BsrFI BsrGI BstAPI BstYI BtgI BtsI ClaI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
HindIII MfeI MmeI MscI MspA1I NcoI PfoI PpuMI
PsiI PstI PvuII SacI SfcI SmlI StyI TatI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmtI BsaI BsaAI BsaHI
BsaXI BsiEI BsiWI BsmI BsmBI BspHI BspMI BsrBI
BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI CspCI DraI DraIII EagI EciI FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MluI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII Tth111I XbaI XmaI ZraI