(Circular) (Six-base) MAPSORT of: pFN21AB6163.seq Check: 1066 from: 1 to: 6508 pFN21AB6163 6508 bp With 182 enzymes: SgfI * April 15, 2010 09:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4318 278 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3530 3851 1937 Size: 1593 321 4594 Fragments arranged by size: 4594 1593 321 Acc65I G'GTAC_C Cuts at: 3463 3829 3463 Size: 366 6142 AclI AA'CG_TT Cuts at: 1563 4635 5008 1563 Size: 3072 373 3063 Fragments arranged by size: 3072 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2373 4571 5955 6223 1632 Size: 741 2198 1384 268 1917 Fragments arranged by size: 2198 1917 1384 741 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6311 AflIII A'CryG_T Cuts at: 1203 5407 6384 1203 Size: 4204 977 1327 Fragments arranged by size: 4204 1327 977 AgeI A'CCGG_T Cuts at: 5339 5339 Size: 6508 AhdI GACnn_n'nnGTC Cuts at: 5237 5237 Size: 6508 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6508 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3248 3258 1184 Size: 2064 10 4434 Fragments arranged by size: 4434 2064 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2867 2899 2867 Size: 32 6476 AlwNI CAG_nnn'CTG Cuts at: 2282 2396 2965 3691 3745 4251 5823 2282 Size: 114 569 726 54 506 1572 2967 Fragments arranged by size: 2967 1572 726 569 506 114 54 ApaI G_GGCC'C Cuts at: 2433 2809 2433 Size: 376 6132 ApaLI G'TGCA_C Cuts at: 3077 4565 5721 3077 Size: 1488 1156 3864 Fragments arranged by size: 3864 1488 1156 ApoI r'AATT_y Cuts at: 1493 3817 4043 4195 6304 1493 Size: 2324 226 152 2109 1697 Fragments arranged by size: 2324 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5062 160 Size: 872 4030 1606 Fragments arranged by size: 4030 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 AvaI C'yCGr_G Cuts at: 1870 1943 2895 3032 3393 3536 3833 1870 Size: 73 952 137 361 143 297 4545 Fragments arranged by size: 4545 952 361 297 143 137 73 AvrII C'CTAG_G Cuts at: 2515 2515 Size: 6508 BamHI G'GATC_C Cuts at: 2453 3838 4205 2453 Size: 1385 367 4756 Fragments arranged by size: 4756 1385 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3050 3463 3829 5284 618 Size: 325 217 627 1263 413 366 1455 1842 Fragments arranged by size: 1842 1455 1263 627 413 366 325 217 BanII G_rGCy'C Cuts at: 1391 2232 2433 2528 2809 2880 2988 3008 3797 Size: 841 201 95 281 71 108 20 789 Cuts at: 3797 3827 1391 Size: 30 4072 Fragments arranged by size: 4072 841 789 281 201 108 95 71 30 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3334 928 Size: 971 1435 4102 Fragments arranged by size: 4102 1435 971 BbvCI CC'TCA_GC Cuts at: 2320 3508 2320 Size: 1188 5320 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3245 3279 4699 4733 1903 Size: 34 1308 34 1420 34 3678 Fragments arranged by size: 3678 1420 1308 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4400 5610 4400 Size: 1210 5298 BclI T'GATC_A Cuts at: 1548 1548 Size: 6508 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3404 5119 136 Size: 107 122 71 631 605 1732 1715 1525 Fragments arranged by size: 1732 1715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3171 6503 3171 Size: 3332 3176 BlpI GC'TnA_GC Cuts at: 2145 3925 2145 Size: 1780 4728 Bme1580I G_kGCm'C Cuts at: 1790 2433 2809 3053 3081 4569 5725 1790 Size: 643 376 244 28 1488 1156 2573 Fragments arranged by size: 2573 1488 1156 643 376 244 28 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6508 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2454 3332 4239 Size: 416 53 83 399 24 1037 878 907 Cuts at: 4239 5197 442 Size: 958 1753 Fragments arranged by size: 1753 1037 958 907 878 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3120 1056 Size: 2064 4444 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2143 3005 3391 2143 Size: 862 386 5260 Fragments arranged by size: 5260 862 386 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2853 3022 3149 3713 5168 1239 Size: 1614 169 127 564 1455 2579 Fragments arranged by size: 2579 1614 1455 564 169 127 Bpu10I CC'TnA_GC Cuts at: 2320 2810 3508 2320 Size: 490 698 5320 Fragments arranged by size: 5320 698 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2171 2253 2840 4633 5498 5796 2171 Size: 82 587 1793 865 298 2883 Fragments arranged by size: 2883 1793 865 587 298 82 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5171 882 Size: 617 3672 2219 Fragments arranged by size: 3672 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4299 493 Size: 711 3095 2702 Fragments arranged by size: 3095 2702 711 BsaBI GATnn'nnATC Cuts at: 6502 6502 Size: 6508 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3665 4315 4697 5332 Size: 53 83 186 936 2132 650 382 635 Cuts at: 5332 275 Size: 1451 Fragments arranged by size: 2132 1451 936 650 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3038 3053 4941 5339 5613 5760 6171 3038 Size: 15 1888 398 274 147 411 3375 Fragments arranged by size: 3375 1888 411 398 274 147 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2404 2407 2434 2437 2867 Size: 30 165 30 1001 3 27 3 430 Cuts at: 2867 2897 3562 3592 1178 Size: 30 665 30 4094 Fragments arranged by size: 4094 1001 665 430 165 30 30 30 30 27 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2428 3152 1186 Size: 1242 724 4542 Fragments arranged by size: 4542 1242 724 BseYI C'CCAG_C Cuts at: 2361 2444 2506 2958 3186 3467 3499 3600 3790 Size: 83 62 452 228 281 32 101 190 Cuts at: 3790 3798 5711 6207 2361 Size: 8 1913 496 2662 Fragments arranged by size: 2662 1913 496 452 281 228 190 101 83 62 32 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3680 1163 Size: 573 1944 3991 Fragments arranged by size: 3991 1944 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2095 3958 4719 5747 1540 Size: 275 186 94 1863 761 1028 2301 Fragments arranged by size: 2301 1863 1028 761 275 186 94 BsiHKAI G_wGCw'C Cuts at: 3081 4569 4654 5725 3081 Size: 1488 85 1071 3864 Fragments arranged by size: 3864 1488 1071 85 BsmI GAATG_Cn' Cuts at: 4019 4112 4019 Size: 93 6415 BsmBI CGTCTCn'nnnn_ Cuts at: 2021 2021 Size: 6508 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2433 2528 2809 2880 2988 3008 Size: 399 442 201 95 281 71 108 20 Cuts at: 3008 3053 3081 3797 3827 4569 4654 5725 1391 Size: 45 28 716 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 716 442 399 281 201 108 95 85 71 45 30 28 20 BspEI T'CCGG_A Cuts at: 6171 6171 Size: 6508 BspHI T'CATG_A Cuts at: 2736 4397 6127 2736 Size: 1661 1730 3117 Fragments arranged by size: 3117 1730 1661 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3744 3863 844 Size: 2900 119 3489 Fragments arranged by size: 3489 2900 119 BsrBI CCG'CTC Cuts at: 1394 1823 2019 2695 3289 4395 1394 Size: 429 196 676 594 1106 3507 Fragments arranged by size: 3507 1106 676 594 429 196 BsrDI GCAATG_nn' Cuts at: 65 1248 3407 5003 5177 65 Size: 1183 2159 1596 174 1396 Fragments arranged by size: 2159 1596 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5152 5339 1700 Size: 110 3342 187 2869 Fragments arranged by size: 3342 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2859 96 Size: 2763 3745 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6508 BssSI C'ACGA_G Cuts at: 4568 5580 4568 Size: 1012 5496 BstAPI GCAn_nnn'nTGC Cuts at: 794 2557 3355 3409 3613 794 Size: 1763 798 54 204 3689 Fragments arranged by size: 3689 1763 798 204 54 BstBI TT'CG_AA Cuts at: 5312 5312 Size: 6508 BstEII G'GTnAC_C Cuts at: 1184 2047 1184 Size: 863 5645 BstXI CCAn_nnnn'nTGG Cuts at: 2539 2539 Size: 6508 BstYI r'GATC_y Cuts at: 1972 2181 2272 2453 3171 3417 3838 4205 4590 Size: 209 91 181 718 246 421 367 385 Cuts at: 4590 4607 6059 6145 6157 6503 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 718 421 385 367 346 246 209 181 91 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3708 513 Size: 552 939 1704 3313 Fragments arranged by size: 3313 1704 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5769 Fragments arranged by size: 5769 683 56 BtsI GCAGTG_nn' Cuts at: 2286 2386 2407 2425 4024 4818 4838 2286 Size: 100 21 18 1599 794 20 3956 Fragments arranged by size: 3956 1599 794 100 21 20 18 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6508 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6473 DraI TTT'AAA Cuts at: 3812 3812 Size: 6508 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5515 809 Size: 507 4199 1802 Fragments arranged by size: 4199 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2664 2931 3955 Size: 54 1425 50 275 18 834 267 1024 Cuts at: 3955 4844 6473 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1024 889 834 275 267 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3955 1537 Size: 275 2143 4090 Fragments arranged by size: 4090 2143 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2392 2929 3296 4438 1353 Size: 1039 537 367 1142 3423 Fragments arranged by size: 3423 1142 1039 537 367 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2840 3314 5095 5469 5615 Size: 1064 138 435 816 474 1781 374 146 Cuts at: 5615 387 Size: 1280 Fragments arranged by size: 1781 1280 1064 816 474 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2373 2853 3022 3149 3713 4571 5168 Size: 393 741 480 169 127 564 858 597 Cuts at: 5168 5955 6223 1239 Size: 787 268 1524 Fragments arranged by size: 1524 858 787 741 597 564 480 393 268 169 127 EcoNI CCTnn'n_nnAGG Cuts at: 2618 2618 Size: 6508 EcoO109I rG'GnC_Cy Cuts at: 2034 2429 2430 2806 3028 3451 3541 3742 2034 Size: 395 1 376 222 423 90 201 4800 Fragments arranged by size: 4800 423 395 376 222 201 90 1 EcoRI G'AATT_C Cuts at: 3817 4195 3817 Size: 378 6130 EcoRV GAT'ATC Cuts at: 3489 3489 Size: 6508 FspI TGC'GCA Cuts at: 3224 5014 3224 Size: 1790 4718 HaeII r_GCGC'y Cuts at: 1678 3600 5655 1678 Size: 1922 2055 2531 Fragments arranged by size: 2531 2055 1922 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2077 2084 2088 2109 2116 Size: 32 135 32 505 7 4 21 7 Cuts at: 2116 2120 2303 2335 2563 2576 2595 2608 2615 Size: 4 183 32 228 13 19 13 7 Cuts at: 2615 2647 2936 2962 2968 2994 3750 3782 5149 Size: 32 289 26 6 26 756 32 1367 Cuts at: 5149 5181 5223 5255 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1367 756 505 289 228 183 135 42 32 32 32 32 32 32 32 26 26 21 19 13 13 7 7 7 6 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3852 4098 669 Size: 988 281 1914 246 3079 Fragments arranged by size: 3079 1914 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6508 HpaI GTT'AAC Cuts at: 4098 4098 Size: 6508 KpnI G_GTAC'C Cuts at: 3467 3833 3467 Size: 366 6142 MfeI C'AATT_G Cuts at: 4107 4107 Size: 6508 MluI A'CGCG_T Cuts at: 6384 6384 Size: 6508 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2967 3605 5622 5806 1305 Size: 1662 638 2017 184 2007 Fragments arranged by size: 2017 2007 1662 638 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2666 10 Size: 54 1425 343 834 3852 Fragments arranged by size: 3852 1425 834 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 4466 4825 4984 518 Size: 648 1494 1806 359 159 2042 Fragments arranged by size: 2042 1806 1494 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2158 2279 2563 2569 3190 3691 3924 4601 Size: 552 121 284 6 621 501 233 677 Cuts at: 4601 5749 5994 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 621 552 501 284 245 233 121 6 NcoI C'CATG_G Cuts at: 513 1065 2004 3708 513 Size: 552 939 1704 3313 Fragments arranged by size: 3313 1704 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6508 NheI G'CTAG_C Cuts at: 1052 3116 1052 Size: 2064 4444 NotI GC'GGCC_GC Cuts at: 3955 3955 Size: 6508 NspI r_CATG'y Cuts at: 1132 1229 2719 2860 3223 3866 5411 1132 Size: 97 1490 141 363 643 1545 2229 Fragments arranged by size: 2229 1545 1490 643 363 141 97 PasI CC'CwG_GG Cuts at: 1348 3455 1348 Size: 2107 4401 PciI A'CATG_T Cuts at: 5407 5407 Size: 6508 PflMI CCAn_nnn'nTGG Cuts at: 1108 3634 1108 Size: 2526 3982 PfoI T'CCnGG_A Cuts at: 2129 2619 2871 3491 2129 Size: 490 252 620 5146 Fragments arranged by size: 5146 620 490 252 PmeI GTTT'AAAC Cuts at: 3812 3812 Size: 6508 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2867 2899 4577 4609 6090 6122 2867 Size: 32 1678 32 1481 32 3253 Fragments arranged by size: 3253 1678 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6508 PsiI TTA'TAA Cuts at: 4078 4078 Size: 6508 PspOMI G'GGCC_C Cuts at: 2429 2805 2429 Size: 376 6132 PstI C_TGCA'G Cuts at: 830 2550 3315 3333 3351 3417 3741 3860 830 Size: 1720 765 18 18 66 324 119 3478 Fragments arranged by size: 3478 1720 765 324 119 66 18 18 PvuI CG_AT'CG Cuts at: 2001 2095 2001 Size: 94 6414 PvuII CAG'CTG Cuts at: 2158 2279 2569 3190 3691 2158 Size: 121 290 621 501 4975 Fragments arranged by size: 4975 621 501 290 121 SalI G'TCGA_C Cuts at: 1936 3850 1936 Size: 1914 4594 SapI GCTCTTCn'nnn_ Cuts at: 3296 3296 Size: 6508 SbfI CC_TGCA'GG Cuts at: 3741 3860 3741 Size: 119 6389 ScaI AGT'ACT Cuts at: 4756 6468 4756 Size: 1712 4796 SfcI C'TryA_G Cuts at: 826 1045 2546 3311 3329 3347 3413 3431 3737 Size: 219 1501 765 18 18 66 18 306 Cuts at: 3737 3856 4991 5672 5863 826 Size: 119 1135 681 191 1471 Fragments arranged by size: 1501 1471 1135 765 681 306 219 191 119 66 18 18 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6508 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6508 SmaI CCC'GGG Cuts at: 1872 2897 3034 3835 1872 Size: 1025 137 801 4545 Fragments arranged by size: 4545 1025 801 137 SmlI C'TyrA_G Cuts at: 820 1017 1943 2186 2268 2819 4612 5513 5775 Size: 197 926 243 82 551 1793 901 262 Cuts at: 5775 820 Size: 1553 Fragments arranged by size: 1793 1553 926 901 551 262 243 197 82 SnaBI TAC'GTA Cuts at: 493 4299 493 Size: 3806 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6508 SphI G_CATG'C Cuts at: 1132 3866 1132 Size: 2734 3774 SspI AAT'ATT Cuts at: 5 52 4432 5 Size: 47 4380 2081 Fragments arranged by size: 4380 2081 47 StuI AGG'CCT Cuts at: 1517 2520 1517 Size: 1003 5505 StyI C'CwwG_G Cuts at: 513 1065 2004 2064 2515 3472 3708 3947 513 Size: 552 939 60 451 957 236 239 3074 Fragments arranged by size: 3074 957 939 552 451 239 236 60 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2092 3461 4535 4552 4705 4890 5335 Size: 1255 512 1369 1074 17 153 185 445 Cuts at: 5335 325 Size: 1498 Fragments arranged by size: 1498 1369 1255 1074 512 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 4754 6466 Size: 274 80 33 51 1443 882 1895 1712 Cuts at: 6466 96 Size: 138 Fragments arranged by size: 1895 1712 1443 882 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2404 2407 2436 2439 3363 3395 3562 3594 6468 Size: 3 29 3 924 32 167 32 2874 Cuts at: 6468 6500 2404 Size: 32 2412 Fragments arranged by size: 2874 2412 924 167 32 32 32 29 3 3 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6508 XbaI T'CTAG_A Cuts at: 3844 3844 Size: 6508 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3635 3635 Size: 6508 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6508 XmaI C'CCGG_G Cuts at: 1870 2895 3032 3833 1870 Size: 1025 137 801 4545 Fragments arranged by size: 4545 1025 801 137 XmnI GAAnn'nnTTC Cuts at: 4637 4637 Size: 6508 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4316 276 Size: 53 83 186 936 2782 2468 Fragments arranged by size: 2782 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI