(Circular) (Six-base) MAPSORT of: pFN21AE0026.seq Check: 9445 from: 1 to: 6160 pFN21AE0026 6160 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3970 278 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3141 3159 3503 1937 Size: 1204 18 344 4594 Fragments arranged by size: 4594 1204 344 18 Acc65I G'GTAC_C Cuts at: 3481 3481 Size: 6160 AclI AA'CG_TT Cuts at: 1563 4287 4660 1563 Size: 2724 373 3063 Fragments arranged by size: 3063 2724 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2303 2339 2480 2918 4223 5607 5875 1632 Size: 671 36 141 438 1305 1384 268 1917 Fragments arranged by size: 1917 1384 1305 671 438 268 141 36 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5963 AflIII A'CryG_T Cuts at: 1203 2974 5059 6036 1203 Size: 1771 2085 977 1327 Fragments arranged by size: 2085 1771 1327 977 AgeI A'CCGG_T Cuts at: 4991 4991 Size: 6160 AhdI GACnn_n'nnGTC Cuts at: 4889 4889 Size: 6160 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6160 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6160 AlwNI CAG_nnn'CTG Cuts at: 2109 2683 2725 2936 3004 3903 5475 2109 Size: 574 42 211 68 899 1572 2794 Fragments arranged by size: 2794 1572 899 574 211 68 42 ApaLI G'TGCA_C Cuts at: 2978 4217 5373 2978 Size: 1239 1156 3765 Fragments arranged by size: 3765 1239 1156 ApoI r'AATT_y Cuts at: 1493 3469 3695 3847 5956 1493 Size: 1976 226 152 2109 1697 Fragments arranged by size: 2109 1976 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4714 160 Size: 872 3682 1606 Fragments arranged by size: 3682 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 AvaI C'yCGr_G Cuts at: 1870 1943 3485 1870 Size: 73 1542 4545 Fragments arranged by size: 4545 1542 73 BamHI G'GATC_C Cuts at: 2008 2926 3490 3857 2008 Size: 918 564 367 4311 Fragments arranged by size: 4311 918 564 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3224 3372 3481 4936 618 Size: 325 217 627 1437 148 109 1455 1842 Fragments arranged by size: 1842 1455 1437 627 325 217 148 109 BanII G_rGCy'C Cuts at: 1391 2082 2191 2706 3479 1391 Size: 691 109 515 773 4072 Fragments arranged by size: 4072 773 691 515 109 BbeI G_GCGC'C Cuts at: 3376 3376 Size: 6160 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2867 2906 928 Size: 971 968 39 4182 Fragments arranged by size: 4182 971 968 39 BbvCI CC'TCA_GC Cuts at: 3197 3197 Size: 6160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4351 4385 1903 Size: 34 2414 34 3678 Fragments arranged by size: 3678 2414 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4052 5262 4052 Size: 1210 4950 BclI T'GATC_A Cuts at: 1548 1548 Size: 6160 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2221 4771 136 Size: 107 122 71 631 605 549 2550 1525 Fragments arranged by size: 2550 1525 631 605 549 122 107 71 BglII A'GATC_T Cuts at: 6155 6155 Size: 6160 BlpI GC'TnA_GC Cuts at: 3577 3577 Size: 6160 Bme1580I G_kGCm'C Cuts at: 1790 2982 4221 5377 1790 Size: 1192 1239 1156 2573 Fragments arranged by size: 2573 1239 1192 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6160 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2170 2888 3091 Size: 416 53 83 399 24 753 718 203 Cuts at: 3091 3891 4849 442 Size: 800 958 1753 Fragments arranged by size: 1753 958 800 753 718 416 399 203 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2705 2873 4820 1239 Size: 1466 168 1947 2579 Fragments arranged by size: 2579 1947 1466 168 Bpu10I CC'TnA_GC Cuts at: 2258 3197 2258 Size: 939 5221 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4285 5150 5448 4285 Size: 865 298 4997 Fragments arranged by size: 4997 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2663 4823 882 Size: 617 1164 2160 2219 Fragments arranged by size: 2219 2160 1164 617 BsaAI yAC'GTr Cuts at: 493 1204 3951 493 Size: 711 2747 2702 Fragments arranged by size: 2747 2702 711 BsaBI GATnn'nnATC Cuts at: 6154 6154 Size: 6160 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3373 3967 4349 4984 Size: 53 83 186 936 1840 594 382 635 Cuts at: 4984 275 Size: 1451 Fragments arranged by size: 1840 1451 936 635 594 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3144 4593 4991 5265 5412 5823 3144 Size: 1449 398 274 147 411 3481 Fragments arranged by size: 3481 1449 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2444 3317 3347 1178 Size: 30 165 30 1011 30 873 30 3991 Fragments arranged by size: 3991 1011 873 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3055 3252 3417 1186 Size: 1869 197 165 3929 Fragments arranged by size: 3929 1869 197 165 BseYI C'CCAG_C Cuts at: 2617 5363 5859 2617 Size: 2746 496 2918 Fragments arranged by size: 2918 2746 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5587 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3610 4371 5399 1540 Size: 275 186 1609 761 1028 2301 Fragments arranged by size: 2301 1609 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2191 2706 2982 4221 4306 5377 2191 Size: 515 276 1239 85 1071 2974 Fragments arranged by size: 2974 1239 1071 515 276 85 BsmI GAATG_Cn' Cuts at: 3671 3764 3671 Size: 93 6067 BsmBI CGTCTCn'nnnn_ Cuts at: 2954 2954 Size: 6160 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2191 2706 2982 3479 4221 4306 Size: 399 292 109 515 276 497 742 85 Cuts at: 4306 5377 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 515 497 399 292 276 109 85 BspEI T'CCGG_A Cuts at: 5823 5823 Size: 6160 BspHI T'CATG_A Cuts at: 2440 4049 5779 2440 Size: 1609 1730 2821 Fragments arranged by size: 2821 1730 1609 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2217 3515 844 Size: 1373 1298 3489 Fragments arranged by size: 3489 1373 1298 BsrBI CCG'CTC Cuts at: 1394 1823 4047 1394 Size: 429 2224 3507 Fragments arranged by size: 3507 2224 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4655 4829 65 Size: 1183 3407 174 1396 Fragments arranged by size: 3407 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3072 3253 4804 4991 1700 Size: 110 1262 181 1551 187 2869 Fragments arranged by size: 2869 1551 1262 187 181 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6160 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6160 BssSI C'ACGA_G Cuts at: 4220 5232 4220 Size: 1012 5148 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6160 BstBI TT'CG_AA Cuts at: 4964 4964 Size: 6160 BstEII G'GTnAC_C Cuts at: 1184 2288 1184 Size: 1104 5056 BstXI CCAn_nnnn'nTGG Cuts at: 2020 2020 Size: 6160 BstYI r'GATC_y Cuts at: 1972 2008 2139 2892 2926 3490 3857 4242 4259 Size: 36 131 753 34 564 367 385 17 Cuts at: 4259 5711 5797 5809 6155 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 753 564 385 367 346 131 86 36 34 17 12 Bsu36I CC'TnA_GG Cuts at: 2072 3067 2072 Size: 995 5165 BtgI C'CryG_G Cuts at: 513 1065 2004 2305 513 Size: 552 939 301 4368 Fragments arranged by size: 4368 939 552 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5421 Fragments arranged by size: 5421 683 56 BtsI GCAGTG_nn' Cuts at: 3420 3676 4470 4490 3420 Size: 256 794 20 5090 Fragments arranged by size: 5090 794 256 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6160 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6125 DraI TTT'AAA Cuts at: 3464 3464 Size: 6160 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5167 809 Size: 507 3851 1802 Fragments arranged by size: 3851 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2098 2229 2643 Size: 54 1425 50 275 18 268 131 414 Cuts at: 2643 3432 3607 4496 6125 8 Size: 789 175 889 1629 43 Fragments arranged by size: 1629 1425 889 789 414 275 268 175 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3607 1537 Size: 275 1795 4090 Fragments arranged by size: 4090 1795 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 4090 1353 Size: 1116 1621 3423 Fragments arranged by size: 3423 1621 1116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4747 5121 5267 387 Size: 1064 138 3158 374 146 1280 Fragments arranged by size: 3158 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2189 2704 2189 Size: 515 5645 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2303 2339 2480 2705 2873 2918 4223 Size: 393 671 36 141 225 168 45 1305 Cuts at: 4223 4820 5607 5875 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1305 787 671 597 393 268 225 168 141 45 36 EcoNI CCTnn'n_nnAGG Cuts at: 2071 2071 Size: 6160 EcoO109I rG'GnC_Cy Cuts at: 2446 2722 3115 2446 Size: 276 393 5491 Fragments arranged by size: 5491 393 276 EcoRI G'AATT_C Cuts at: 3469 3847 3469 Size: 378 5782 FspI TGC'GCA Cuts at: 4666 4666 Size: 6160 HaeII r_GCGC'y Cuts at: 1678 2305 3376 5307 1678 Size: 627 1071 1931 2531 Fragments arranged by size: 2531 1931 1071 627 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2178 2210 3317 3349 4801 Size: 32 135 32 606 32 1107 32 1452 Cuts at: 4801 4833 4875 4907 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1452 1107 606 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3504 3750 669 Size: 988 281 1566 246 3079 Fragments arranged by size: 3079 1566 988 281 246 HindIII A'AGCT_T Cuts at: 748 2486 748 Size: 1738 4422 HpaI GTT'AAC Cuts at: 3750 3750 Size: 6160 KasI G'GCGC_C Cuts at: 3372 3372 Size: 6160 KpnI G_GTAC'C Cuts at: 3485 3485 Size: 6160 MfeI C'AATT_G Cuts at: 3759 3759 Size: 6160 MluI A'CGCG_T Cuts at: 6036 6036 Size: 6160 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5274 5458 1305 Size: 3969 184 2007 Fragments arranged by size: 3969 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2100 2231 2645 3434 10 Size: 54 1425 343 268 131 414 789 2736 Fragments arranged by size: 2736 1425 789 414 343 268 131 54 MslI CAynn'nnrTG Cuts at: 518 1166 2060 4118 4477 4636 518 Size: 648 894 2058 359 159 2042 Fragments arranged by size: 2058 2042 894 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2118 2227 2617 2656 2666 2775 2912 3576 Size: 512 109 390 39 10 109 137 664 Cuts at: 3576 4253 5401 5646 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 512 390 245 137 109 109 39 10 NaeI GCC'GGC Cuts at: 3074 3074 Size: 6160 NarI GG'CG_CC Cuts at: 3373 3373 Size: 6160 NcoI C'CATG_G Cuts at: 513 1065 2004 2305 513 Size: 552 939 301 4368 Fragments arranged by size: 4368 939 552 301 NdeI CA'TA_TG Cuts at: 387 387 Size: 6160 NgoMIV G'CCGG_C Cuts at: 3072 3072 Size: 6160 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6160 NotI GC'GGCC_GC Cuts at: 3607 3607 Size: 6160 NspI r_CATG'y Cuts at: 1132 1229 3518 5063 1132 Size: 97 2289 1545 2229 Fragments arranged by size: 2289 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3368 1348 Size: 2020 4140 PciI A'CATG_T Cuts at: 5059 5059 Size: 6160 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6160 PfoI T'CCnGG_A Cuts at: 2269 2269 Size: 6160 PmeI GTTT'AAAC Cuts at: 3464 3464 Size: 6160 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4229 4261 5742 5774 4229 Size: 32 1481 32 4615 Fragments arranged by size: 4615 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2722 2722 Size: 6160 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6160 PsiI TTA'TAA Cuts at: 3730 3730 Size: 6160 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2150 2182 2150 Size: 32 6128 PstI C_TGCA'G Cuts at: 830 2366 3512 830 Size: 1536 1146 3478 Fragments arranged by size: 3478 1536 1146 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6160 PvuII CAG'CTG Cuts at: 2118 2227 2617 2912 2118 Size: 109 390 295 5366 Fragments arranged by size: 5366 390 295 109 SacI G_AGCT'C Cuts at: 2191 2706 2191 Size: 515 5645 SalI G'TCGA_C Cuts at: 1936 3502 1936 Size: 1566 4594 SbfI CC_TGCA'GG Cuts at: 3512 3512 Size: 6160 ScaI AGT'ACT Cuts at: 4408 6120 4408 Size: 1712 4448 SfcI C'TryA_G Cuts at: 826 1045 2362 2651 3400 3508 4643 5324 5515 Size: 219 1317 289 749 108 1135 681 191 Cuts at: 5515 826 Size: 1471 Fragments arranged by size: 1471 1317 1135 749 681 289 219 191 108 SfoI GGC'GCC Cuts at: 3374 3374 Size: 6160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6160 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6160 SmaI CCC'GGG Cuts at: 1872 3487 1872 Size: 1615 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4264 5165 5427 820 Size: 197 926 2321 901 262 1553 Fragments arranged by size: 2321 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3951 493 Size: 3458 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6160 SphI G_CATG'C Cuts at: 1132 3518 1132 Size: 2386 3774 SspI AAT'ATT Cuts at: 5 52 4084 5 Size: 47 4032 2081 Fragments arranged by size: 4032 2081 47 StuI AGG'CCT Cuts at: 1517 2360 2480 2767 1517 Size: 843 120 287 4910 Fragments arranged by size: 4910 843 287 120 StyI C'CwwG_G Cuts at: 513 1065 2004 2019 2201 2305 3599 513 Size: 552 939 15 182 104 1294 3074 Fragments arranged by size: 3074 1294 939 552 182 104 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3175 4187 4204 4357 4542 4987 325 Size: 1255 1595 1012 17 153 185 445 1498 Fragments arranged by size: 1595 1498 1255 1012 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2424 2875 4406 Size: 274 80 33 51 1443 447 451 1531 Cuts at: 4406 6118 96 Size: 1712 138 Fragments arranged by size: 1712 1531 1443 451 447 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 3171 3203 3267 3299 6120 6152 2414 Size: 32 725 32 64 32 2821 32 2422 Fragments arranged by size: 2821 2422 725 64 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6160 XbaI T'CTAG_A Cuts at: 3496 3496 Size: 6160 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2020 2680 3442 2020 Size: 660 762 4738 Fragments arranged by size: 4738 762 660 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6160 XmaI C'CCGG_G Cuts at: 1870 3485 1870 Size: 1615 4545 XmnI GAAnn'nnTTC Cuts at: 2324 4289 2324 Size: 1965 4195 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3968 276 Size: 53 83 186 936 2434 2468 Fragments arranged by size: 2468 2434 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI