Restriction map for
pFN21AE0023
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccII AccIII AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI ApaI ApaLI AscI Asp718I
AspEI AspI AvaI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BbvI BfrI BglI BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmFI
BsmI BsoBI BspDI BspEI BspHI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO109I FauI
Fnu4HI FseI FspI HgaI Hin1I
ItaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI TseI Tth111I
Van91I XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 994 GTAGAC GT^[AC][GT]AC
AclI 962 AACGTT AA^CGTT
Aor51HI 1353 AGCGCT AGC^GCT
AseI 820 ATTAAT AT^TAAT
AsnI 820 ATTAAT AT^TAAT
BalI 1944 TGGCCA TGG^CCA
BbsI 2809 GAAGAC GAAGAC(2/6)
BciVI 1275 GTATCC GTATCC(6/5)
BclI 975 TGATCA T^GATCA
BpuAI 2809 GAAGAC GAAGAC(2/6)
BsaAI 515 CACGTA [CT]AC^GT[GA]
Bsp1407I 2616 TGTACA T^GTACA
BspMI 597 GCAGGT ACCTGC(4/8)
BsrGI 2616 TGTACA T^GTACA
BstAPI 82 GCAACCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 15 GGTCACC G^GT[ACGT]ACC
BstPI 15 GGTCACC G^GT[ACGT]ACC
BstXI 1314 CCAAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EaeI 1944 TGGCCA [CT]^GGCC[GA]
Eco47III 1353 AGCGCT AGC^GCT
EcoO65I 15 GGTCACC G^GT[ACGT]ACC
EcoRV 2274 GATATC GAT^ATC
FbaI 975 TGATCA T^GATCA
HincII 784 GTTAAC GT[CT]^[GA]AC
HindII 784 GTTAAC GT[CT]^[GA]AC
HindIII 2110 AAGCTT A^AGCTT
HpaI 784 GTTAAC GTT^AAC
MluNI 1944 TGGCCA TGG^CCA
MscI 1944 TGGCCA TGG^CCA
NdeI 1057 CATATG CA^TATG
PshBI 820 ATTAAT AT^TAAT
Psp1406I 962 AACGTT AA^CGTT
PstI 52 CTGCAG CTGCA^G
SapI 2494 GCTCTTC GCTCTTC(1/4)
ScaI 1431 AGTACT AGT^ACT
SspBI 2616 TGTACA T^GTACA
XbaI 2436 TCTAGA T^CTAGA
XcmI 2390 CCAATTTTAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 31 enzymes) #
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AflIII 131 ACATGT A^C[GA][CT]GT
2004 ACATGT
AspHI 3 GAGCTC G[AT]GC[AT]^C
1779 GAGCTC
BcgI 268 CGAAAACATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2690 GCACGTCCATCG
BcnI 78 CCCGG CC^[GC]GG
2340 CCCGG
BglII 613 AGATCT A^GATCT
1197 AGATCT
BsaI 1018 GAGACC GGTCTC(1/5)
2714 GGTCTC
BseRI 1331 GAGGAG GAGGAG(10/8)
2188 GAGGAG
BsiHKAI 3 GAGCTC G[AT]GC[AT]^C
1779 GAGCTC
BspLU11I 131 ACATGT A^CATGT
2004 ACATGT
BstF5I 44 GGATG GGATG(2/0)
1839 GGATG
BstNI 73 CCAGG CC^[AT]GG
355 CCTGG
CfoI 58 GCGC GCG^C
1354 GCGC
EcoRII 73 CCAGG ^CC[AT]GG
355 CCTGG
EcoT14I 829 CCAAGG C^C[AT][AT]GG
1346 CCTTGG
EcoT22I 492 ATGCAT ATGCA^T
2419 ATGCAT
FokI 44 GGATG GGATG(9/13)
1839 GGATG
HaeII 57 GGCGCT [GA]GCGC^[CT]
1353 AGCGCT
HapII 79 CCGG C^CGG
2341 CCGG
HhaI 58 GCGC GCG^C
1354 GCGC
HinP1I 58 GCGC G^CGC
1354 GCGC
HpaII 79 CCGG C^CGG
2341 CCGG
MspI 79 CCGG C^CGG
2341 CCGG
MvaI 73 CCAGG CC^[AT]GG
355 CCTGG
NciI 78 CCCGG CC^[GC]GG
2340 CCCGG
NsiI 492 ATGCAT ATGCA^T
2419 ATGCAT
PleI 1185 GACTC GAGTC(4/5)
1702 GACTC
SacI 3 GAGCTC GAGCT^C
1779 GAGCTC
SfcI 52 CTGCAG C^T[GA][CT]AG
1193 CTGTAG
SmlI 326 CTCAAG C^T[CT][GA]AG
609 CTTGAG
SspI 823 AATATT AAT^ATT
2202 AATATT
StyI 829 CCAAGG C^C[AT][AT]GG
1346 CCTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BanII BfaI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BsrDI BsrI BssKI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EarI EcoRI HaeIII HinfI
HphI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MwoI NdeII NlaIII NlaIV
NspI RsaI Sau3AI Sau96I ScrFI
SfaNI TaqI TfiI Tru9I Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI