Restriction map for
pFN21AE0023
(Circular) (Six-base) MAPSORT of: pFN21AE0023.seq Check: 6750 from: 1 to: 7570
pFN21AE0023 7570 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5380 278
Size: 53 83 186 936 3844 2468
Fragments arranged by size:
3844 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3000 4913 1937
Size: 1063 1913 4594
Fragments arranged by size:
4594 1913 1063
Acc65I G'GTAC_C
Cuts at: 4891 4891
Size: 7570
AclI AA'CG_TT
Cuts at: 1563 2968 5697 6070 1563
Size: 1405 2729 373 3063
Fragments arranged by size:
3063 2729 1405 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2706 3693 3987 4544 5633 7017 7285 1632
Size: 1074 987 294 557 1089 1384 268 1917
Fragments arranged by size:
1917 1384 1089 1074 987 557 294 268
AfeI AGC'GCT
Cuts at: 3360 3360
Size: 7570
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7373
AflIII A'CryG_T
Cuts at: 1203 2136 4009 6469 7446 1203
Size: 933 1873 2460 977 1327
Fragments arranged by size:
2460 1873 1327 977 933
AgeI A'CCGG_T
Cuts at: 6401 6401
Size: 7570
AhdI GACnn_n'nnGTC
Cuts at: 6299 6299
Size: 7570
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7570
AlwNI CAG_nnn'CTG
Cuts at: 5313 6885 5313
Size: 1572 5998
ApaLI G'TGCA_C
Cuts at: 5627 6783 5627
Size: 1156 6414
ApoI r'AATT_y
Cuts at: 1493 2228 2267 2440 2538 2694 3029 3149 3506
Size: 735 39 173 98 156 335 120 357
Cuts at: 3506 3520 3902 4267 4567 4879 5105 5257 7366
Size: 14 382 365 300 312 226 152 2109
Cuts at: 7366 1493
Size: 1697
Fragments arranged by size:
2109 1697 735 382 365 357 335 312
300 226 173 156 152 120 98 39
14
AseI AT'TA_AT
Cuts at: 160 1032 2826 6124 160
Size: 872 1794 3298 1606
Fragments arranged by size:
3298 1794 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
AvaI C'yCGr_G
Cuts at: 1870 1943 4895 1870
Size: 73 2952 4545
Fragments arranged by size:
4545 2952 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3921 3954 3921
Size: 33 7537
BamHI G'GATC_C
Cuts at: 4900 5267 4900
Size: 367 7203
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4891 6346 618
Size: 325 217 627 3104 1455 1842
Fragments arranged by size:
3104 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2012 3788 4607 4889 1391
Size: 621 1776 819 282 4072
Fragments arranged by size:
4072 1776 819 621 282
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4821 928
Size: 971 2922 3677
Fragments arranged by size:
3677 2922 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2262 2296 4684 4718 5761 5795 1903
Size: 34 325 34 2388 34 1043 34 3678
Fragments arranged by size:
3678 2388 1043 325 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3291 5462 6672 3291
Size: 2171 1210 4189
Fragments arranged by size:
4189 2171 1210
BclI T'GATC_A
Cuts at: 1548 2980 1548
Size: 1432 6138
BfrBI ATG'CAT
Cuts at: 2499 4426 2499
Size: 1927 5643
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6181 136
Size: 107 122 71 631 605 4509 1525
Fragments arranged by size:
4509 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2618 3202 7565 2618
Size: 584 4363 2623
Fragments arranged by size:
4363 2623 584
BlpI GC'TnA_GC
Cuts at: 4987 4987
Size: 7570
Bme1580I G_kGCm'C
Cuts at: 1790 5631 6787 1790
Size: 3841 1156 2573
Fragments arranged by size:
3841 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4698 1318
Size: 3380 4190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 5301 6259 442
Size: 416 53 83 399 24 3884 958 1753
Fragments arranged by size:
3884 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4162 4626 4162
Size: 464 7106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3741 4427 4458 4731 6230 1239
Size: 2502 686 31 273 1499 2579
Fragments arranged by size:
2579 2502 1499 686 273 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2316 2635 5695 6560 6858 2316
Size: 319 3060 865 298 3028
Fragments arranged by size:
3060 3028 865 319 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3017 4725 6233 882
Size: 617 1518 1708 1508 2219
Fragments arranged by size:
2219 1708 1518 1508 617
BsaAI yAC'GTr
Cuts at: 493 1204 2522 5361 493
Size: 711 1318 2839 2702
Fragments arranged by size:
2839 2702 1318 711
BsaBI GATnn'nnATC
Cuts at: 7564 7564
Size: 7570
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5377 5759 6394 275
Size: 53 83 186 936 3844 382 635 1451
Fragments arranged by size:
3844 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6003 6401 6675 6822 7233 6003
Size: 398 274 147 411 6340
Fragments arranged by size:
6340 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7345
Fragments arranged by size:
7345 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3351 4208 1186
Size: 2165 857 4548
Fragments arranged by size:
4548 2165 857
BseYI C'CCAG_C
Cuts at: 6773 7269 6773
Size: 496 7074
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6997
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5020 5781 6809 1540
Size: 275 186 3019 761 1028 2301
Fragments arranged by size:
3019 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2012 3788 5631 5716 6787 2012
Size: 1776 1843 85 1071 2795
Fragments arranged by size:
2795 1843 1776 1071 85
BsmI GAATG_Cn'
Cuts at: 5081 5174 5081
Size: 93 7477
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 3788 4607 4889 5631 5716 6787
Size: 399 222 1776 819 282 742 85 1071
Cuts at: 6787 1391
Size: 2174
Fragments arranged by size:
2174 1776 1071 819 742 399 282 222
85
BspEI T'CCGG_A
Cuts at: 7233 7233
Size: 7570
BspHI T'CATG_A
Cuts at: 5459 7189 5459
Size: 1730 5840
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2593 4925 844
Size: 1749 2332 3489
Fragments arranged by size:
3489 2332 1749
BsrBI CCG'CTC
Cuts at: 1394 1823 5457 1394
Size: 429 3634 3507
Fragments arranged by size:
3634 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2091 2278 2418 2499 6065 6239 65
Size: 1183 843 187 140 81 3566 174 1396
Fragments arranged by size:
3566 1396 1183 843 187 174 140 81
BsrFI r'CCGG_y
Cuts at: 1700 1810 6214 6401 1700
Size: 110 4404 187 2869
Fragments arranged by size:
4404 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4621 96
Size: 4525 3045
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7570
BssSI C'ACGA_G
Cuts at: 5630 6642 5630
Size: 1012 6558
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2093 794
Size: 1299 6271
BstBI TT'CG_AA
Cuts at: 6374 6374
Size: 7570
BstEII G'GTnAC_C
Cuts at: 1184 2020 1184
Size: 836 6734
BstXI CCAn_nnnn'nTGG
Cuts at: 3326 3326
Size: 7570
BstYI r'GATC_y
Cuts at: 1972 2618 3052 3202 4900 5267 5652 5669 7121
Size: 646 434 150 1698 367 385 17 1452
Cuts at: 7121 7207 7219 7565 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1698 1452 646 434 385 367 346
150 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 7018
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6831
Fragments arranged by size:
6831 683 56
BtsI GCAGTG_nn'
Cuts at: 4192 5086 5880 5900 4192
Size: 894 794 20 5862
Fragments arranged by size:
5862 894 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2469 2504 529
Size: 35 1905 35 5595
Fragments arranged by size:
5595 1905 35 35
DraI TTT'AAA
Cuts at: 4309 4402 4806 4874 4309
Size: 93 404 68 7005
Fragments arranged by size:
7005 404 93 68
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6577 809
Size: 507 5261 1802
Fragments arranged by size:
5261 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3949 5017 5906
Size: 54 1425 50 275 18 2119 1068 889
Cuts at: 5906 7535 8
Size: 1629 43
Fragments arranged by size:
2119 1629 1425 1068 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5017 1537
Size: 275 3205 4090
Fragments arranged by size:
4090 3205 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3624 3963 4185 4443 4506 5500 1353
Size: 2271 339 222 258 63 994 3423
Fragments arranged by size:
3423 2271 994 339 258 222 63
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6157 6531 6677 387
Size: 1064 138 4568 374 146 1280
Fragments arranged by size:
4568 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2010 3786 2010
Size: 1776 5794
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2706 3693 3741 3987 4427 4458 4544
Size: 393 1074 987 48 246 440 31 86
Cuts at: 4544 4731 5633 6230 7017 7285 1239
Size: 187 902 597 787 268 1524
Fragments arranged by size:
1524 1074 987 902 787 597 440 393
268 246 187 86 48 31
EcoRI G'AATT_C
Cuts at: 2267 4267 4567 4879 5257 2267
Size: 2000 300 312 378 4580
Fragments arranged by size:
4580 2000 378 312 300
EcoRV GAT'ATC
Cuts at: 4281 4281
Size: 7570
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3170 3170
Size: 7570
FspI TGC'GCA
Cuts at: 6076 6076
Size: 7570
HaeII r_GCGC'y
Cuts at: 1678 2066 3362 6717 1678
Size: 388 1296 3355 2531
Fragments arranged by size:
3355 2531 1296 388
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3227 3259 3448 3480 6211
Size: 32 135 32 1655 32 189 32 2731
Cuts at: 6211 6243 6285 6317 1373
Size: 32 42 32 2626
Fragments arranged by size:
2731 2626 1655 189 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2791 4914 5160 669
Size: 988 281 853 2123 246 3079
Fragments arranged by size:
3079 2123 988 853 281 246
HindIII A'AGCT_T
Cuts at: 748 4115 748
Size: 3367 4203
HpaI GTT'AAC
Cuts at: 2791 5160 2791
Size: 2369 5201
KpnI G_GTAC'C
Cuts at: 4895 4895
Size: 7570
MfeI C'AATT_G
Cuts at: 5169 5169
Size: 7570
MluI A'CGCG_T
Cuts at: 7446 7446
Size: 7570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3167 6684 6868 1305
Size: 1862 3517 184 2007
Fragments arranged by size:
3517 2007 1862 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3951 10
Size: 54 1425 343 2119 3629
Fragments arranged by size:
3629 2119 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2283 3455 4029 5528 5887 6046 518
Size: 648 1117 1172 574 1499 359 159 2042
Fragments arranged by size:
2042 1499 1172 1117 648 574 359 159
MspA1I CmG'CkG
Cuts at: 1606 4986 5663 6811 7056 1606
Size: 3380 677 1148 245 2120
Fragments arranged by size:
3380 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7018
NdeI CA'TA_TG
Cuts at: 387 3063 387
Size: 2676 4894
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7570
NotI GC'GGCC_GC
Cuts at: 5017 5017
Size: 7570
NsiI A_TGCA'T
Cuts at: 2501 4428 2501
Size: 1927 5643
NspI r_CATG'y
Cuts at: 1132 1229 2140 4013 4782 4928 6473 1132
Size: 97 911 1873 769 146 1545 2229
Fragments arranged by size:
2229 1873 1545 911 769 146 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7570
PciI A'CATG_T
Cuts at: 2136 4009 6469 2136
Size: 1873 2460 3237
Fragments arranged by size:
3237 2460 1873
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7570
PfoI T'CCnGG_A
Cuts at: 4344 4344
Size: 7570
PmeI GTTT'AAAC
Cuts at: 4874 4874
Size: 7570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5639 5671 7152 7184 5639
Size: 32 1481 32 6025
Fragments arranged by size:
6025 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7570
PsiI TTA'TAA
Cuts at: 5140 5140
Size: 7570
PstI C_TGCA'G
Cuts at: 830 2061 4922 830
Size: 1231 2861 3478
Fragments arranged by size:
3478 2861 1231
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7570
SacI G_AGCT'C
Cuts at: 2012 3788 2012
Size: 1776 5794
SalI G'TCGA_C
Cuts at: 1936 4912 1936
Size: 2976 4594
SapI GCTCTTCn'nnn_
Cuts at: 4506 4506
Size: 7570
SbfI CC_TGCA'GG
Cuts at: 4922 4922
Size: 7570
ScaI AGT'ACT
Cuts at: 3438 5818 7530 3438
Size: 2380 1712 3478
Fragments arranged by size:
3478 2380 1712
SfcI C'TryA_G
Cuts at: 826 1045 2057 3198 4918 6053 6734 6925 826
Size: 219 1012 1141 1720 1135 681 191 1471
Fragments arranged by size:
1720 1471 1141 1135 1012 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7570
SmaI CCC'GGG
Cuts at: 1872 4897 1872
Size: 3025 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2331 2614 5674 6575 6837 820
Size: 197 926 388 283 3060 901 262 1553
Fragments arranged by size:
3060 1553 926 901 388 283 262 197
SnaBI TAC'GTA
Cuts at: 493 5361 493
Size: 4868 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7570
SphI G_CATG'C
Cuts at: 1132 4928 1132
Size: 3796 3774
SspI AAT'ATT
Cuts at: 5 52 2830 4209 5494 5
Size: 47 2778 1379 1285 2081
Fragments arranged by size:
2778 2081 1379 1285 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7570
StyI C'CwwG_G
Cuts at: 513 1065 2834 3351 5009 513
Size: 552 1769 517 1658 3074
Fragments arranged by size:
3074 1769 1658 552 517
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2008 2697 2764 4169 5597 5614 5767
Size: 1255 428 689 67 1405 1428 17 153
Cuts at: 5767 5952 6397 325
Size: 185 445 1498
Fragments arranged by size:
1498 1428 1405 1255 689 445 428 185
153 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3436 4621 5816
Size: 274 80 33 51 1443 1459 1185 1195
Cuts at: 5816 7528 96
Size: 1712 138
Fragments arranged by size:
1712 1459 1443 1195 1185 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2094 2126 2293 2325 7530 7562 2094
Size: 32 167 32 5205 32 2102
Fragments arranged by size:
5205 2102 167 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7570
XbaI T'CTAG_A
Cuts at: 4441 4906 4441
Size: 465 7105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4402 4402
Size: 7570
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7570
XmaI C'CCGG_G
Cuts at: 1870 4895 1870
Size: 3025 4545
XmnI GAAnn'nnTTC
Cuts at: 2694 3834 4294 5699 2694
Size: 1140 460 1405 4565
Fragments arranged by size:
4565 1405 1140 460
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5378 276
Size: 53 83 186 936 3844 2468
Fragments arranged by size:
3844 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I
BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI