Restriction map for
pFN21AE1011
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AfaI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaII AviII AvrII BalI BamHI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I Cac8I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AciI 56 CCGC CCGC(-3/-1)
AflIII 656 ACATGT A^C[GA][CT]GT
AvaI 600 CTCGGG C^[CT]CG[GA]G
BanI 145 GGCACC G^G[CT][GA]CC
BcnI 437 CCCGG CC^[GC]GG
BpmI 564 CTCCAG CTGGAG(16/14)
BsoBI 600 CTCGGG C^[CT]CG[GA]G
BspLU11I 656 ACATGT A^CATGT
BspMI 51 ACCTGC ACCTGC(4/8)
BsrI 566 CCAGT ACTGG(1/-1)
BstAPI 122 GCAGCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 29 CCAGG CC^[AT]GG
BstYI 357 AGATCC [GA]^GATC[CT]
Cfr13I 434 GGCCC G^G[ACGT]CC
DraI 115 TTTAAA TTT^AAA
DraII 433 AGGCCCC [GA]G^G[ACGT]CC[CT]
DsaI 5 CCATGG C^C[GA][CT]GG
EarI 18 GAAGAG CTCTTC(1/4)
EcoO109I 433 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRII 29 CCAGG ^CC[AT]GG
EcoT14I 5 CCATGG C^C[AT][AT]GG
HapII 438 CCGG C^CGG
HgaI 83 GCGTC GACGC(5/10)
HpaII 438 CCGG C^CGG
HphI 497 TCACC GGTGA(8/7)
Ksp632I 18 GAAGAG CTCTTC(1/4)
MflI 357 AGATCC [GA]^GATC[CT]
MspA1I 178 CAGCTG C[AC]G^C[GT]G
MspI 438 CCGG C^CGG
MvaI 29 CCAGG CC^[AT]GG
NciI 437 CCCGG CC^[GC]GG
NcoI 5 CCATGG C^CATGG
NspI 656 ACATGT [GA]CATG^[CT]
PleI 528 GACTC GAGTC(4/5)
PvuII 178 CAGCTG CAG^CTG
SapI 18 GAAGAGC GCTCTTC(1/4)
Sau96I 434 GGCCC G^G[ACGT]CC
SfaNI 125 GCATC GCATC(5/9)
SmlI 429 CTCAAG C^T[CT][GA]AG
StyI 5 CCATGG C^C[AT][AT]GG
TaqI 541 TCGA T^CGA
TfiI 676 GAATC G^A[AT]TC
TthHB8I 541 TCGA T^CGA
XhoII 357 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
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AluI 179 AGCT AG^CT
598 AGCT
AlwI 358 GATCC GGATC(4/5)
167 GGATC
AlwNI 123 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
479 CAGTAACTG
AspHI 459 GAGCAC G[AT]GC[AT]^C
612 GAGCAC
BanII 21 GAGCCC G[GA]GC[CT]^C
624 GGGCTC
BbvI 122 GCAGC GCAGC(8/12)
596 GCAGC
BfaI 299 CTAG C^TAG
365 CTAG
BseRI 534 CTCCTC GAGGAG(10/8)
627 CTCCTC
BsiHKAI 459 GAGCAC G[AT]GC[AT]^C
612 GAGCAC
BsiYI 225 CCCTAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
566 CCAGTGCAGGG
BslI 225 CCCTAAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
566 CCAGTGCAGGG
BssKI 29 CCAGG ^CC[ACGT]GG
437 CCCGG
BstF5I 545 CATCC GGATG(2/0)
551 CATCC
DdeI 176 CTCAG C^T[ACGT]AG
275 CTGAG
DpnII 168 GATC ^GATC
358 GATC
FokI 545 CATCC GGATG(9/13)
551 CATCC
HaeIII 32 GGCC GG^CC
434 GGCC
HinfI 528 GACTC G^A[ACGT]TC
676 GAATC
MaeI 299 CTAG C^TAG
365 CTAG
MaeII 449 ACGT A^CGT
494 ACGT
MboI 168 GATC ^GATC
358 GATC
MboII 18 GAAGA GAAGA(8/7)
237 GAAGA
MseI 116 TTAA T^TAA
518 TTAA
MwoI 46 GCGAAACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
122 GCAGCATCTGC
NdeII 168 GATC ^GATC
358 GATC
NlaIII 6 CATG CATG^
657 CATG
Sau3AI 168 GATC ^GATC
358 GATC
ScrFI 29 CCAGG CC^[ACGT]GG
437 CCCGG
Tru9I 116 TTAA T^TAA
518 TTAA
TseI 122 GCAGC G^C[AT]GC
596 GCAGC
Tsp45I 496 GTCAC ^GT[GC]AC
559 GTGAC
Tsp509I 172 AATT ^AATT
291 AATT
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
BmyI BsaJI BsgI Bsp1286I Fnu4HI
ItaI MaeIII MnlI NlaIV TspRI