Restriction map for
pFN21AE1011
(Circular) (Six-base) MAPSORT of: pFN21AE1011.seq Check: 4997 from: 1 to: 5389
pFN21AE1011 5389 bp
With 182 enzymes: SgfI *
April 15, 2010 09:36 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3199 278
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2732 1937
Size: 795 4594
Acc65I G'GTAC_C
Cuts at: 2710 2710
Size: 5389
AclI AA'CG_TT
Cuts at: 1563 3516 3889 1563
Size: 1953 373 3063
Fragments arranged by size:
3063 1953 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2083 2133 3452 4836 5104 1632
Size: 451 50 1319 1384 268 1917
Fragments arranged by size:
1917 1384 1319 451 268 50
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5192
AflIII A'CryG_T
Cuts at: 1203 2661 4288 5265 1203
Size: 1458 1627 977 1327
Fragments arranged by size:
1627 1458 1327 977
AgeI A'CCGG_T
Cuts at: 4220 4220
Size: 5389
AhdI GACnn_n'nnGTC
Cuts at: 4118 4118
Size: 5389
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5389
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5389
AlwNI CAG_nnn'CTG
Cuts at: 2133 2489 3132 4704 2133
Size: 356 643 1572 2818
Fragments arranged by size:
2818 1572 643 356
ApaLI G'TGCA_C
Cuts at: 3446 4602 3446
Size: 1156 4233
ApoI r'AATT_y
Cuts at: 1493 2698 2924 3076 5185 1493
Size: 1205 226 152 2109 1697
Fragments arranged by size:
2109 1697 1205 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3943 160
Size: 872 2911 1606
Fragments arranged by size:
2911 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
AvaI C'yCGr_G
Cuts at: 1870 1943 2605 2714 1870
Size: 73 662 109 4545
Fragments arranged by size:
4545 662 109 73
BamHI G'GATC_C
Cuts at: 2719 3086 2719
Size: 367 5022
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2710 4165 618
Size: 325 217 627 363 560 1455 1842
Fragments arranged by size:
1842 1455 627 560 363 325 217
BanII G_rGCy'C
Cuts at: 1391 2030 2633 2708 1391
Size: 639 603 75 4072
Fragments arranged by size:
4072 639 603 75
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4418
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3580 3614 1903
Size: 34 1643 34 3678
Fragments arranged by size:
3678 1643 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3281 4491 3281
Size: 1210 4179
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5389
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4000 136
Size: 107 122 71 631 605 2328 1525
Fragments arranged by size:
2328 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5384 5384
Size: 5389
BlpI GC'TnA_GC
Cuts at: 2806 2806
Size: 5389
Bme1580I G_kGCm'C
Cuts at: 1790 3450 4606 1790
Size: 1660 1156 2573
Fragments arranged by size:
2573 1660 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5389
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3120 4078 442
Size: 416 53 83 399 24 1703 958 1753
Fragments arranged by size:
1753 1703 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5389
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2554 4049 1239
Size: 1315 1495 2579
Fragments arranged by size:
2579 1495 1315
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2419 3514 4379 4677 2419
Size: 1095 865 298 3131
Fragments arranged by size:
3131 1095 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4052 882
Size: 617 2553 2219
Fragments arranged by size:
2553 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3180 493
Size: 711 1976 2702
Fragments arranged by size:
2702 1976 711
BsaBI GATnn'nnATC
Cuts at: 5383 5383
Size: 5389
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3196 3578 4213 275
Size: 53 83 186 936 1663 382 635 1451
Fragments arranged by size:
1663 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3822 4220 4494 4641 5052 3822
Size: 398 274 147 411 4159
Fragments arranged by size:
4159 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2615 2645 1178
Size: 30 165 30 1212 30 3922
Fragments arranged by size:
3922 1212 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2530 2623 1186
Size: 1344 93 3952
Fragments arranged by size:
3952 1344 93
BseYI C'CCAG_C
Cuts at: 4592 5088 4592
Size: 496 4893
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2077 2454 2595 1163
Size: 573 341 377 141 3957
Fragments arranged by size:
3957 573 377 341 141
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2839 3600 4628 1540
Size: 275 186 838 761 1028 2301
Fragments arranged by size:
2301 1028 838 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2468 2621 3450 3535 4606 2468
Size: 153 829 85 1071 3251
Fragments arranged by size:
3251 1071 829 153 85
BsmI GAATG_Cn'
Cuts at: 2900 2993 2900
Size: 93 5296
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2030 2468 2621 2633 2708 3450 3535
Size: 399 240 438 153 12 75 742 85
Cuts at: 3535 4606 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 438 399 240 153 85
75 12
BspEI T'CCGG_A
Cuts at: 5052 5052
Size: 5389
BspHI T'CATG_A
Cuts at: 3278 5008 3278
Size: 1730 3659
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2065 2744 844
Size: 1221 679 3489
Fragments arranged by size:
3489 1221 679
BsrBI CCG'CTC
Cuts at: 1394 1823 3276 1394
Size: 429 1453 3507
Fragments arranged by size:
3507 1453 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3884 4058 65
Size: 1183 2636 174 1396
Fragments arranged by size:
2636 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4033 4220 1700
Size: 110 2223 187 2869
Fragments arranged by size:
2869 2223 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5389
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5389
BssSI C'ACGA_G
Cuts at: 3449 4461 3449
Size: 1012 4377
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2133 794
Size: 1339 4050
BstBI TT'CG_AA
Cuts at: 4193 4193
Size: 5389
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5389
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 5389
BstYI r'GATC_y
Cuts at: 1972 2362 2719 3086 3471 3488 4940 5026 5038
Size: 390 357 367 385 17 1452 86 12
Cuts at: 5038 5384 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 390 385 367 357 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2010 513
Size: 552 945 3892
Fragments arranged by size:
3892 945 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2151 487
Size: 683 56 925 3725
Fragments arranged by size:
3725 925 683 56
BtsI GCAGTG_nn'
Cuts at: 2466 2905 3699 3719 2466
Size: 439 794 20 4136
Fragments arranged by size:
4136 794 439 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5354
DraI TTT'AAA
Cuts at: 2122 2693 2122
Size: 571 4818
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4396 809
Size: 507 3080 1802
Fragments arranged by size:
3080 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2836 3725 5354
Size: 54 1425 50 275 18 1006 889 1629
Cuts at: 5354 8
Size: 43
Fragments arranged by size:
1629 1425 1006 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2836 1537
Size: 275 1024 4090
Fragments arranged by size:
4090 1024 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2018 3319 1353
Size: 665 1301 3423
Fragments arranged by size:
3423 1301 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3976 4350 4496 387
Size: 1064 138 2387 374 146 1280
Fragments arranged by size:
2387 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2083 2133 2554 3452 4049 4836 5104
Size: 393 451 50 421 898 597 787 268
Cuts at: 5104 1239
Size: 1524
Fragments arranged by size:
1524 898 787 597 451 421 393 268
50
EcoO109I rG'GnC_Cy
Cuts at: 2439 2439
Size: 5389
EcoRI G'AATT_C
Cuts at: 2698 3076 2698
Size: 378 5011
FspI TGC'GCA
Cuts at: 3895 3895
Size: 5389
HaeII r_GCGC'y
Cuts at: 1678 4536 1678
Size: 2858 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2164 2196 2615 2647 4030
Size: 32 135 32 592 32 419 32 1383
Cuts at: 4030 4062 4104 4136 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1383 592 419 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2733 2979 669
Size: 988 281 795 246 3079
Fragments arranged by size:
3079 988 795 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5389
HpaI GTT'AAC
Cuts at: 2979 2979
Size: 5389
KpnI G_GTAC'C
Cuts at: 2714 2714
Size: 5389
MfeI C'AATT_G
Cuts at: 2988 2988
Size: 5389
MluI A'CGCG_T
Cuts at: 5265 5265
Size: 5389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4503 4687 1305
Size: 3198 184 2007
Fragments arranged by size:
3198 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3567
Fragments arranged by size:
3567 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3347 3706 3865 518
Size: 648 843 1338 359 159 2042
Fragments arranged by size:
2042 1338 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2185 2805 3482 4630 4875 1606
Size: 579 620 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 620 579 245
NcoI C'CATG_G
Cuts at: 513 1065 2010 513
Size: 552 945 3892
Fragments arranged by size:
3892 945 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5389
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5389
NotI GC'GGCC_GC
Cuts at: 2836 2836
Size: 5389
NspI r_CATG'y
Cuts at: 1132 1229 2665 2747 4292 1132
Size: 97 1436 82 1545 2229
Fragments arranged by size:
2229 1545 1436 97 82
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5389
PciI A'CATG_T
Cuts at: 2661 4288 2661
Size: 1627 3762
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5389
PmeI GTTT'AAAC
Cuts at: 2693 2693
Size: 5389
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3458 3490 4971 5003 3458
Size: 32 1481 32 3844
Fragments arranged by size:
3844 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5389
PsiI TTA'TAA
Cuts at: 2959 2959
Size: 5389
PstI C_TGCA'G
Cuts at: 830 2741 830
Size: 1911 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5389
PvuII CAG'CTG
Cuts at: 2185 2185
Size: 5389
SalI G'TCGA_C
Cuts at: 1936 2731 1936
Size: 795 4594
SapI GCTCTTCn'nnn_
Cuts at: 2018 2018
Size: 5389
SbfI CC_TGCA'GG
Cuts at: 2741 2741
Size: 5389
ScaI AGT'ACT
Cuts at: 3637 5349 3637
Size: 1712 3677
SfcI C'TryA_G
Cuts at: 826 1045 2737 3872 4553 4744 826
Size: 219 1692 1135 681 191 1471
Fragments arranged by size:
1692 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5389
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5389
SmaI CCC'GGG
Cuts at: 1872 2716 1872
Size: 844 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2434 3493 4394 4656 820
Size: 197 926 491 1059 901 262 1553
Fragments arranged by size:
1553 1059 926 901 491 262 197
SnaBI TAC'GTA
Cuts at: 493 3180 493
Size: 2687 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5389
SphI G_CATG'C
Cuts at: 1132 2747 1132
Size: 1615 3774
SspI AAT'ATT
Cuts at: 5 52 3313 5
Size: 47 3261 2081
Fragments arranged by size:
3261 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5389
StyI C'CwwG_G
Cuts at: 513 1065 2010 2828 513
Size: 552 945 818 3074
Fragments arranged by size:
3074 945 818 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3416 3433 3586 3771 4216 325
Size: 1255 1836 17 153 185 445 1498
Fragments arranged by size:
1836 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3635 5347 96
Size: 274 80 33 51 1443 1658 1712 138
Fragments arranged by size:
1712 1658 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5349 5381 5349
Size: 32 5357
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5389
XbaI T'CTAG_A
Cuts at: 2725 2725
Size: 5389
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5389
XmaI C'CCGG_G
Cuts at: 1870 2714 1870
Size: 844 4545
XmnI GAAnn'nnTTC
Cuts at: 3518 3518
Size: 5389
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3197 276
Size: 53 83 186 936 1663 2468
Fragments arranged by size:
2468 1663 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI