Restriction map for
pFN21AE0014
(Linear) (Six-base) MAPSORT of: KIEE0014.seq Check: 7031 from: 1 to: 1461
KIEE0014.orf
With 182 enzymes: SgfI *
April 24, 2009 13:09 ..
AatII G_ACGT'C
Cuts at: 0 100 1461
Size: 100 1361
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 794 1461
Size: 280 514 667
Fragments arranged by size:
667 514 280
AflII C'TTAA_G
Cuts at: 0 1342 1461
Size: 1342 119
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 832 853 1461
Size: 832 21 608
Fragments arranged by size:
832 608 21
AlwNI CAG_nnn'CTG
Cuts at: 0 75 123 1461
Size: 75 48 1338
Fragments arranged by size:
1338 75 48
ApaI G_GGCC'C
Cuts at: 0 602 1461
Size: 602 859
ApoI r'AATT_y
Cuts at: 0 498 1159 1461
Size: 498 661 302
Fragments arranged by size:
661 498 302
BanI G'GyrC_C
Cuts at: 0 994 1428 1461
Size: 994 434 33
Fragments arranged by size:
994 434 33
BanII G_rGCy'C
Cuts at: 0 602 1213 1461
Size: 602 611 248
Fragments arranged by size:
611 602 248
BbsI GAAGACnn'nnnn_
Cuts at: 0 764 782 1461
Size: 764 18 679
Fragments arranged by size:
764 679 18
BbvCI CC'TCA_GC
Cuts at: 0 9 1461
Size: 9 1452
BciVI GTATCCnnnnn_n'
Cuts at: 0 376 1129 1461
Size: 376 753 332
Fragments arranged by size:
753 376 332
BclI T'GATC_A
Cuts at: 0 131 1461
Size: 131 1330
BfrBI ATG'CAT
Cuts at: 0 884 1461
Size: 884 577
BglI GCCn_nnn'nGGC
Cuts at: 0 1033 1461
Size: 1033 428
BlpI GC'TnA_GC
Cuts at: 0 76 739 1461
Size: 76 663 722
Fragments arranged by size:
722 663 76
Bme1580I G_kGCm'C
Cuts at: 0 602 1461
Size: 602 859
BmrI ACTGGGnnnn_n'
Cuts at: 0 597 1461
Size: 597 864
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1461
Size: 1231 230
Bpu10I CC'TnA_GC
Cuts at: 0 9 1219 1387 1461
Size: 9 1210 168 74
Fragments arranged by size:
1210 168 74 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1042 1461
Size: 676 366 419
Fragments arranged by size:
676 419 366
BsaI GGTCTCn'nnnn_
Cuts at: 0 190 1096 1461
Size: 190 906 365
Fragments arranged by size:
906 365 190
BsaAI yAC'GTr
Cuts at: 0 535 1328 1461
Size: 535 793 133
Fragments arranged by size:
793 535 133
BsaHI Gr'CG_yC
Cuts at: 0 4 97 1461
Size: 4 93 1364
Fragments arranged by size:
1364 93 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 1461
Size: 757 704
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1111 1461
Size: 655 456 350
Fragments arranged by size:
655 456 350
Bsp1286I G_dGCh'C
Cuts at: 0 602 1213 1461
Size: 602 611 248
Fragments arranged by size:
611 602 248
BsrDI GCAATG_nn'
Cuts at: 0 998 1430 1461
Size: 998 432 31
Fragments arranged by size:
998 432 31
BsrFI r'CCGG_y
Cuts at: 0 400 1461
Size: 400 1061
BsrGI T'GTAC_A
Cuts at: 0 812 842 1461
Size: 812 30 619
Fragments arranged by size:
812 619 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1125 1461
Size: 1125 336
BstEII G'GTnAC_C
Cuts at: 0 151 1461
Size: 151 1310
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 1461
Size: 147 1314
BtsI GCAGTG_nn'
Cuts at: 0 332 392 733 1461
Size: 332 60 341 728
Fragments arranged by size:
728 341 332 60
DraIII CAC_nnn'GTG
Cuts at: 0 436 1461
Size: 436 1025
EaeI y'GGCC_r
Cuts at: 0 1229 1371 1461
Size: 1229 142 90
Fragments arranged by size:
1229 142 90
EarI CTCTTCn'nnn_
Cuts at: 0 220 1294 1327 1461
Size: 220 1074 33 134
Fragments arranged by size:
1074 220 134 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 794 1231 1461
Size: 280 514 437 230
Fragments arranged by size:
514 437 280 230
EcoO109I rG'GnC_Cy
Cuts at: 0 584 598 599 1461
Size: 584 14 1 862
Fragments arranged by size:
862 584 14 1
EcoRI G'AATT_C
Cuts at: 0 498 1159 1461
Size: 498 661 302
Fragments arranged by size:
661 498 302
EcoRV GAT'ATC
Cuts at: 0 414 1461
Size: 414 1047
HaeII r_GCGC'y
Cuts at: 0 43 124 699 1461
Size: 43 81 575 762
Fragments arranged by size:
762 575 81 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1127 1159 1461
Size: 1127 32 302
Fragments arranged by size:
1127 302 32
HincII GTy'rAC
Cuts at: 0 471 1461
Size: 471 990
MfeI C'AATT_G
Cuts at: 0 239 1461
Size: 239 1222
MscI TGG'CCA
Cuts at: 0 1373 1461
Size: 1373 88
MslI CAynn'nnrTG
Cuts at: 0 814 958 1461
Size: 814 144 503
Fragments arranged by size:
814 503 144
MspA1I CmG'CkG
Cuts at: 0 13 806 1461
Size: 13 793 655
Fragments arranged by size:
793 655 13
NsiI A_TGCA'T
Cuts at: 0 886 1461
Size: 886 575
NspI r_CATG'y
Cuts at: 0 813 819 1461
Size: 813 6 642
Fragments arranged by size:
813 642 6
PasI CC'CwG_GG
Cuts at: 0 102 1461
Size: 102 1359
PflMI CCAn_nnn'nTGG
Cuts at: 0 1028 1283 1461
Size: 1028 255 178
Fragments arranged by size:
1028 255 178
PfoI T'CCnGG_A
Cuts at: 0 68 1461
Size: 68 1393
PmlI CAC'GTG
Cuts at: 0 1328 1461
Size: 1328 133
PspOMI G'GGCC_C
Cuts at: 0 598 1461
Size: 598 863
PstI C_TGCA'G
Cuts at: 0 59 1461
Size: 59 1402
SapI GCTCTTCn'nnn_
Cuts at: 0 1294 1461
Size: 1294 167
SexAI A'CCwGG_T
Cuts at: 0 1139 1461
Size: 1139 322
SfcI C'TryA_G
Cuts at: 0 55 1461
Size: 55 1406
SmlI C'TyrA_G
Cuts at: 0 691 1057 1342 1461
Size: 691 366 285 119
Fragments arranged by size:
691 366 285 119
StuI AGG'CCT
Cuts at: 0 233 1461
Size: 233 1228
StyI C'CwwG_G
Cuts at: 0 1065 1368 1432 1461
Size: 1065 303 64 29
Fragments arranged by size:
1065 303 64 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1020 1461
Size: 1020 441
TatI w'GTAC_w
Cuts at: 0 812 842 1461
Size: 812 30 619
Fragments arranged by size:
812 619 30
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1029 1461
Size: 1029 432
ZraI GAC'GTC
Cuts at: 0 98 1461
Size: 98 1363
Enzymes that do cut:
AatII AcuI AflII AlfI AlwNI ApaI ApoI BanI
BanII BbsI BbvCI BciVI BclI BfrBI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BseRI BsgI Bsp1286I BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BtsI DraIII EaeI EarI Eco57MI EcoO109I EcoRI
EcoRV HaeII Hin4I HincII MfeI MscI MslI MspA1I
NsiI NspI PasI PflMI PfoI PmlI PspOMI PstI
SapI SexAI SfcI SmlI StuI StyI TaqII TatI
XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BglII BmgBI BmtI BplI
BsaBI BsaWI BsaXI BseYI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BssHII BssSI BstBI
BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DrdI EagI EciI EcoICRI EcoNI FalI FseI FspI
FspAI HindIII HpaI KasI KpnI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PciI PmeI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI
XmaI XmnI