(Circular) (Six-base) MAPSORT of: pFN21AE0012.seq Check: 9923 from: 1 to: 6268 pFN21AE0012 6268 bp With 182 enzymes: SgfI * April 15, 2010 09:25 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4078 278 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3611 1937 Size: 1674 4594 Acc65I G'GTAC_C Cuts at: 2080 3589 2080 Size: 1509 4759 AclI AA'CG_TT Cuts at: 1563 4395 4768 1563 Size: 2832 373 3063 Fragments arranged by size: 3063 2832 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2199 2801 3089 4331 5715 5983 1632 Size: 567 602 288 1242 1384 268 1917 Fragments arranged by size: 1917 1384 1242 602 567 288 268 AflII C'TTAA_G Cuts at: 820 1017 2189 820 Size: 197 1172 4899 Fragments arranged by size: 4899 1172 197 AflIII A'CryG_T Cuts at: 1203 2932 5167 6144 1203 Size: 1729 2235 977 1327 Fragments arranged by size: 2235 1729 1327 977 AgeI A'CCGG_T Cuts at: 5099 5099 Size: 6268 AhdI GACnn_n'nnGTC Cuts at: 4997 4997 Size: 6268 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6268 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6268 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2003 2035 2003 Size: 32 6236 AlwNI CAG_nnn'CTG Cuts at: 4011 5583 4011 Size: 1572 4696 ApaLI G'TGCA_C Cuts at: 4325 5481 4325 Size: 1156 5112 ApoI r'AATT_y Cuts at: 1493 3362 3434 3448 3577 3803 3955 6064 1493 Size: 1869 72 14 129 226 152 2109 1697 Fragments arranged by size: 2109 1869 1697 226 152 129 72 14 AseI AT'TA_AT Cuts at: 160 1032 4822 160 Size: 872 3790 1606 Fragments arranged by size: 3790 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 AvaI C'yCGr_G Cuts at: 1870 1943 3593 1870 Size: 73 1650 4545 Fragments arranged by size: 4545 1650 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2973 3006 2973 Size: 33 6235 BamHI G'GATC_C Cuts at: 3598 3965 3598 Size: 367 5901 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2080 3589 5044 618 Size: 325 217 627 293 1509 1455 1842 Fragments arranged by size: 1842 1509 1455 627 325 293 217 BanII G_rGCy'C Cuts at: 1391 3587 1391 Size: 2196 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3095 928 Size: 971 1196 4101 Fragments arranged by size: 4101 1196 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4459 4493 1903 Size: 34 50 34 2438 34 3678 Fragments arranged by size: 3678 2438 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4160 5370 4160 Size: 1210 5058 BclI T'GATC_A Cuts at: 1548 2329 2596 1548 Size: 781 267 5220 Fragments arranged by size: 5220 781 267 BfrBI ATG'CAT Cuts at: 2226 2226 Size: 6268 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2382 2926 4879 Size: 107 122 71 631 605 710 544 1953 Cuts at: 4879 136 Size: 1525 Fragments arranged by size: 1953 1525 710 631 605 544 122 107 71 BglII A'GATC_T Cuts at: 3178 6263 3178 Size: 3085 3183 BlpI GC'TnA_GC Cuts at: 2666 3685 2666 Size: 1019 5249 Bme1580I G_kGCm'C Cuts at: 1790 2922 4329 5485 1790 Size: 1132 1407 1156 2573 Fragments arranged by size: 2573 1407 1156 1132 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6268 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2017 3249 3999 Size: 416 53 83 399 24 600 1232 750 Cuts at: 3999 4957 442 Size: 958 1753 Fragments arranged by size: 1753 1232 958 750 600 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6268 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4928 1239 Size: 3689 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 4393 5258 5556 2927 Size: 1466 865 298 3639 Fragments arranged by size: 3639 1466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4931 882 Size: 617 3432 2219 Fragments arranged by size: 3432 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4059 493 Size: 711 2855 2702 Fragments arranged by size: 2855 2702 711 BsaBI GATnn'nnATC Cuts at: 3062 6262 3062 Size: 3200 3068 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4075 4457 5092 275 Size: 53 83 186 936 2542 382 635 1451 Fragments arranged by size: 2542 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4701 5099 5373 5520 5931 4701 Size: 398 274 147 411 5038 Fragments arranged by size: 5038 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3105 3135 1178 Size: 30 165 30 1702 30 4311 Fragments arranged by size: 4311 1702 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2441 1186 Size: 1255 5013 BseYI C'CCAG_C Cuts at: 5471 5967 5471 Size: 496 5772 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5695 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3718 4479 5507 1540 Size: 275 186 1717 761 1028 2301 Fragments arranged by size: 2301 1717 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4329 4414 5485 4329 Size: 85 1071 5112 Fragments arranged by size: 5112 1071 85 BsmI GAATG_Cn' Cuts at: 2378 3779 3872 2378 Size: 1401 93 4774 Fragments arranged by size: 4774 1401 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2922 3587 4329 4414 5485 1391 Size: 399 1132 665 742 85 1071 2174 Fragments arranged by size: 2174 1132 1071 742 665 399 85 BspEI T'CCGG_A Cuts at: 5931 5931 Size: 6268 BspHI T'CATG_A Cuts at: 2866 4157 5887 2866 Size: 1291 1730 3247 Fragments arranged by size: 3247 1730 1291 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2151 2410 3623 844 Size: 1307 259 1213 3489 Fragments arranged by size: 3489 1307 1213 259 BsrBI CCG'CTC Cuts at: 1394 1823 4155 1394 Size: 429 2332 3507 Fragments arranged by size: 3507 2332 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2544 4763 4937 65 Size: 1183 1296 2219 174 1396 Fragments arranged by size: 2219 1396 1296 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4912 5099 1700 Size: 110 3102 187 2869 Fragments arranged by size: 3102 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6268 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6268 BssSI C'ACGA_G Cuts at: 4328 5340 4328 Size: 1012 5256 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6268 BstBI TT'CG_AA Cuts at: 5072 5072 Size: 6268 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6268 BstYI r'GATC_y Cuts at: 1972 2140 3178 3549 3598 3965 4350 4367 5819 Size: 168 1038 371 49 367 385 17 1452 Cuts at: 5819 5905 5917 6263 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1038 385 371 367 346 168 86 49 17 12 Bsu36I CC'TnA_GG Cuts at: 3138 3138 Size: 6268 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5716 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5529 Fragments arranged by size: 5529 683 56 BtsI GCAGTG_nn' Cuts at: 3784 4578 4598 3784 Size: 794 20 5454 Fragments arranged by size: 5454 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6268 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6233 DraI TTT'AAA Cuts at: 2088 3151 3340 3572 2088 Size: 1063 189 232 4784 Fragments arranged by size: 4784 1063 232 189 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2537 5275 809 Size: 507 1221 2738 1802 Fragments arranged by size: 2738 1802 1221 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 2770 3715 Size: 54 1425 50 275 18 561 379 945 Cuts at: 3715 4604 6233 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 945 889 561 379 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3715 1537 Size: 275 1903 4090 Fragments arranged by size: 4090 1903 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3086 3429 4198 1353 Size: 1733 343 769 3423 Fragments arranged by size: 3423 1733 769 343 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4855 5229 5375 387 Size: 1064 138 3266 374 146 1280 Fragments arranged by size: 3266 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2199 2801 3089 4331 4928 5715 5983 Size: 393 567 602 288 1242 597 787 268 Cuts at: 5983 1239 Size: 1524 Fragments arranged by size: 1524 1242 787 602 597 567 393 288 268 EcoNI CCTnn'n_nnAGG Cuts at: 2955 2955 Size: 6268 EcoO109I rG'GnC_Cy Cuts at: 2495 2495 Size: 6268 EcoRI G'AATT_C Cuts at: 3577 3955 3577 Size: 378 5890 EcoRV GAT'ATC Cuts at: 2572 2572 Size: 6268 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2204 2458 2204 Size: 254 6014 FspI TGC'GCA Cuts at: 4774 4774 Size: 6268 HaeII r_GCGC'y Cuts at: 1678 5415 1678 Size: 3737 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4909 4941 4983 5015 1373 Size: 32 135 32 3337 32 42 32 2626 Fragments arranged by size: 3337 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3612 3858 669 Size: 988 281 1674 246 3079 Fragments arranged by size: 3079 1674 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6268 HpaI GTT'AAC Cuts at: 3858 3858 Size: 6268 KpnI G_GTAC'C Cuts at: 2084 3593 2084 Size: 1509 4759 MfeI C'AATT_G Cuts at: 3320 3867 3320 Size: 547 5721 MluI A'CGCG_T Cuts at: 6144 6144 Size: 6268 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2198 5382 5566 1305 Size: 893 3184 184 2007 Fragments arranged by size: 3184 2007 893 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2393 2772 10 Size: 54 1425 343 561 379 3506 Fragments arranged by size: 3506 1425 561 379 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 2604 3215 4226 4585 4744 518 Size: 648 1108 330 611 1011 359 159 2042 Fragments arranged by size: 2042 1108 1011 648 611 359 330 159 MspA1I CmG'CkG Cuts at: 1606 3197 3684 4361 5509 5754 1606 Size: 1591 487 677 1148 245 2120 Fragments arranged by size: 2120 1591 1148 677 487 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5716 NdeI CA'TA_TG Cuts at: 387 387 Size: 6268 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6268 NotI GC'GGCC_GC Cuts at: 3715 3715 Size: 6268 NsiI A_TGCA'T Cuts at: 2228 2228 Size: 6268 NspI r_CATG'y Cuts at: 1132 1229 2455 2936 3626 5171 1132 Size: 97 1226 481 690 1545 2229 Fragments arranged by size: 2229 1545 1226 690 481 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6268 PciI A'CATG_T Cuts at: 2932 5167 2932 Size: 2235 4033 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6268 PmeI GTTT'AAAC Cuts at: 3572 3572 Size: 6268 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4337 4369 5850 5882 4337 Size: 32 1481 32 4723 Fragments arranged by size: 4723 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2495 2495 Size: 6268 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6268 PsiI TTA'TAA Cuts at: 2244 3838 2244 Size: 1594 4674 PstI C_TGCA'G Cuts at: 830 3620 830 Size: 2790 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6268 PvuII CAG'CTG Cuts at: 3197 3197 Size: 6268 SalI G'TCGA_C Cuts at: 1936 3610 1936 Size: 1674 4594 SapI GCTCTTCn'nnn_ Cuts at: 3429 3429 Size: 6268 SbfI CC_TGCA'GG Cuts at: 3620 3620 Size: 6268 ScaI AGT'ACT Cuts at: 4516 6228 4516 Size: 1712 4556 SfcI C'TryA_G Cuts at: 826 1045 2827 3616 4751 5432 5623 826 Size: 219 1782 789 1135 681 191 1471 Fragments arranged by size: 1782 1471 1135 789 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6268 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6268 SmaI CCC'GGG Cuts at: 1872 3595 1872 Size: 1723 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2189 2942 4372 5273 5535 820 Size: 197 926 246 753 1430 901 262 1553 Fragments arranged by size: 1553 1430 926 901 753 262 246 197 SnaBI TAC'GTA Cuts at: 493 4059 493 Size: 3566 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6268 SphI G_CATG'C Cuts at: 1132 2455 3626 1132 Size: 1323 1171 3774 Fragments arranged by size: 3774 1323 1171 SspI AAT'ATT Cuts at: 5 52 4192 5 Size: 47 4140 2081 Fragments arranged by size: 4140 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6268 StyI C'CwwG_G Cuts at: 513 1065 2804 2850 3707 513 Size: 552 1739 46 857 3074 Fragments arranged by size: 3074 1739 857 552 46 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4295 4312 4465 4650 5095 325 Size: 1255 2715 17 153 185 445 1498 Fragments arranged by size: 2715 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 4514 6226 Size: 274 80 33 51 1443 846 1691 1712 Cuts at: 6226 96 Size: 138 Fragments arranged by size: 1712 1691 1443 846 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6228 6260 6228 Size: 32 6236 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6268 XbaI T'CTAG_A Cuts at: 3604 3604 Size: 6268 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2514 2514 Size: 6268 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6268 XmaI C'CCGG_G Cuts at: 1870 3593 1870 Size: 1723 4545 XmnI GAAnn'nnTTC Cuts at: 3024 3366 4397 3024 Size: 342 1031 4895 Fragments arranged by size: 4895 1031 342 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4076 276 Size: 53 83 186 936 2542 2468 Fragments arranged by size: 2542 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI