Restriction map for
pFN21AE0009
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AvaI AviII BbeI BbrPI BbvCI
BclI BfrI BglI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsoBI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BssHII BssSI Bst1107I BstBI BstUI
BstXI BstZ17I CelII Cfr10I CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AlwNI 1874 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 526 CCTCAGG CC^T[ACGT]AGG
AspI 1729 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 76 CCTAGG C^CTAGG
BalI 1662 TGGCCA TGG^CCA
BamHI 1643 GGATCC G^GATCC
BanI 425 GGTGCC G^G[CT][GA]CC
BbsI 1379 GTCTTC GAAGAC(2/6)
BcgI 989 GCAATTCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1811 GGATAC GTATCC(6/5)
BglII 95 AGATCT A^GATCT
BlnI 76 CCTAGG C^CTAGG
BpuAI 1379 GTCTTC GAAGAC(2/6)
BsaI 619 GAGACC GGTCTC(1/5)
Bsp1407I 329 TGTACA T^GTACA
BspDI 1219 ATCGAT AT^CGAT
BspMI 367 ACCTGC ACCTGC(4/8)
BsrGI 329 TGTACA T^GTACA
BstAPI 362 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1254 GGTCACC G^GT[ACGT]ACC
BstPI 1254 GGTCACC G^GT[ACGT]ACC
Bsu36I 526 CCTCAGG CC^T[ACGT]AGG
CfoI 876 GCGC GCG^C
ClaI 1219 ATCGAT AT^CGAT
DraII 803 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1662 TGGCCA [CT]^GGCC[GA]
Eco81I 526 CCTCAGG CC^T[ACGT]AGG
EcoNI 859 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 803 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1254 GGTCACC G^GT[ACGT]ACC
EcoRI 161 GAATTC G^AATTC
HgaI 1341 GCGTC GACGC(5/10)
HhaI 876 GCGC GCG^C
HinP1I 876 GCGC G^CGC
HincII 208 GTTAAC GT[CT]^[GA]AC
HindII 208 GTTAAC GT[CT]^[GA]AC
HindIII 1102 AAGCTT A^AGCTT
HpaI 208 GTTAAC GTT^AAC
MfeI 1165 CAATTG C^AATTG
MluNI 1662 TGGCCA TGG^CCA
MscI 1662 TGGCCA TGG^CCA
MslI 321 CATAACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1165 CAATTG C^AATTG
PflFI 1729 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 803 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 915 CAGCTG CAG^CTG
SacI 979 GAGCTC GAGCT^C
SapI 1323 GAAGAGC GCTCTTC(1/4)
ScaI 1617 AGTACT AGT^ACT
SfcI 903 CTACAG C^T[GA][CT]AG
SspBI 329 TGTACA T^GTACA
Tth111I 1729 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 891 GTGCTC G[AT]GC[AT]^C
979 GAGCTC
BanII 355 GAGCCC G[GA]GC[CT]^C
979 GAGCTC
BcnI 462 CCCGG CC^[GC]GG
807 CCCGG
BsgI 360 CTGCAC GTGCAG(16/14)
1609 CTGCAC
BsiHKAI 891 GTGCTC G[AT]GC[AT]^C
979 GAGCTC
BsrI 985 ACTGG ACTGG(1/-1)
187 CCAGT
BstYI 95 AGATCT [GA]^GATC[CT]
1643 GGATCC
Cac8I 711 GCTGGC GC[ACGT]^[ACGT]GC
878 GCCTGC
EarI 386 GAAGAG CTCTTC(1/4)
1323 GAAGAG
FauI 846 CCCGC CCCGC(4/6)
887 GCGGG
HapII 463 CCGG C^CGG
808 CCGG
HpaII 463 CCGG C^CGG
808 CCGG
Ksp632I 386 GAAGAG CTCTTC(1/4)
1323 GAAGAG
MaeII 1482 ACGT A^CGT
1580 ACGT
MflI 95 AGATCT [GA]^GATC[CT]
1643 GGATCC
MspA1I 915 CAGCTG C[AC]G^C[GT]G
949 CCGCTG
MspI 463 CCGG C^CGG
808 CCGG
NciI 462 CCCGG CC^[GC]GG
807 CCCGG
SfaNI 1876 GATGC GCATC(5/9)
920 GCATC
StuI 691 AGGCCT AGG^CCT
1677 AGGCCT
TaqI 840 TCGA T^CGA
1220 TCGA
Tsp45I 1255 GTCAC ^GT[GC]AC
1894 GTGAC
TthHB8I 840 TCGA T^CGA
1220 TCGA
XhoII 95 AGATCT [GA]^GATC[CT]
1643 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AvaII BbvI BfaI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI StyI
TfiI Tru9I TseI Tsp509I TspRI
XbaI XmnI