(Circular) (Six-base) MAPSORT of: pFN21AE0009.seq Check: 7833 from: 1 to: 6634 pFN21AE0009 6634 bp With 182 enzymes: SgfI * April 15, 2010 09:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4444 278 Size: 53 83 186 936 2908 2468 Fragments arranged by size: 2908 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3977 1937 Size: 2040 4594 Acc65I G'GTAC_C Cuts at: 3955 3955 Size: 6634 AclI AA'CG_TT Cuts at: 1563 4761 5134 1563 Size: 3198 373 3063 Fragments arranged by size: 3198 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2472 3066 3134 3200 4697 6081 6349 1632 Size: 840 594 68 66 1497 1384 268 1917 Fragments arranged by size: 1917 1497 1384 840 594 268 68 66 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6437 AflIII A'CryG_T Cuts at: 1203 5533 6510 1203 Size: 4330 977 1327 Fragments arranged by size: 4330 1327 977 AgeI A'CCGG_T Cuts at: 5465 5465 Size: 6634 AhdI GACnn_n'nnGTC Cuts at: 5363 5363 Size: 6634 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6634 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6634 AlwNI CAG_nnn'CTG Cuts at: 3884 4377 5949 3884 Size: 493 1572 4569 Fragments arranged by size: 4569 1572 493 ApaLI G'TGCA_C Cuts at: 4691 5847 4691 Size: 1156 5478 ApoI r'AATT_y Cuts at: 1493 2076 2166 3040 3362 3444 3943 4169 4321 Size: 583 90 874 322 82 499 226 152 Cuts at: 4321 6430 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 874 583 499 322 226 152 90 82 AseI AT'TA_AT Cuts at: 160 1032 5188 160 Size: 872 4156 1606 Fragments arranged by size: 4156 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6634 AvaI C'yCGr_G Cuts at: 1870 1943 3959 1870 Size: 73 2016 4545 Fragments arranged by size: 4545 2016 73 AvrII C'CTAG_G Cuts at: 2081 2081 Size: 6634 BamHI G'GATC_C Cuts at: 3648 3964 4331 3648 Size: 316 367 5951 Fragments arranged by size: 5951 367 316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2430 3955 5410 618 Size: 325 217 627 643 1525 1455 1842 Fragments arranged by size: 1842 1525 1455 643 627 325 217 BanII G_rGCy'C Cuts at: 1391 2364 2988 3953 1391 Size: 973 624 965 4072 Fragments arranged by size: 4072 973 965 624 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3377 928 Size: 971 1478 4185 Fragments arranged by size: 4185 1478 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2983 3017 4825 4859 1903 Size: 34 1046 34 1808 34 3678 Fragments arranged by size: 3678 1808 1046 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3810 4526 5736 3810 Size: 716 1210 4708 Fragments arranged by size: 4708 1210 716 BclI T'GATC_A Cuts at: 1548 1548 Size: 6634 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5245 136 Size: 107 122 71 631 605 3573 1525 Fragments arranged by size: 3573 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2100 6629 2100 Size: 4529 2105 BlpI GC'TnA_GC Cuts at: 4051 4051 Size: 6634 Bme1580I G_kGCm'C Cuts at: 1790 2435 4695 5851 1790 Size: 645 2260 1156 2573 Fragments arranged by size: 2573 2260 1156 645 BmgBI CAC'GTC Cuts at: 1318 3586 1318 Size: 2268 4366 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4365 5323 442 Size: 416 53 83 399 24 2948 958 1753 Fragments arranged by size: 2948 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6634 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 2721 3002 5294 1239 Size: 1047 435 281 2292 2579 Fragments arranged by size: 2579 2292 1047 435 281 Bpu10I CC'TnA_GC Cuts at: 2358 2358 Size: 6634 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4759 5624 5922 4759 Size: 865 298 5471 Fragments arranged by size: 5471 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2618 5297 882 Size: 617 1119 2679 2219 Fragments arranged by size: 2679 2219 1119 617 BsaAI yAC'GTr Cuts at: 493 1204 4425 493 Size: 711 3221 2702 Fragments arranged by size: 3221 2702 711 BsaBI GATnn'nnATC Cuts at: 6628 6628 Size: 6634 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4441 4823 5458 275 Size: 53 83 186 936 2908 382 635 1451 Fragments arranged by size: 2908 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5067 5465 5739 5886 6297 5067 Size: 398 274 147 411 5404 Fragments arranged by size: 5404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2225 2255 3725 3755 1178 Size: 30 165 30 822 30 1470 30 4057 Fragments arranged by size: 4057 1470 822 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2520 2594 2691 2694 2720 2725 2762 2765 Size: 1334 74 97 3 26 5 37 3 Cuts at: 2765 2768 2777 2780 2783 2792 2801 2810 2819 Size: 3 9 3 3 9 9 9 9 Cuts at: 2819 2835 2853 1186 Size: 16 18 4967 Fragments arranged by size: 4967 1334 97 74 37 26 18 16 9 9 9 9 9 5 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 5837 6333 5837 Size: 496 6138 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2350 3599 1163 Size: 573 614 1249 4198 Fragments arranged by size: 4198 1249 614 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4084 4845 5873 1540 Size: 275 186 2083 761 1028 2301 Fragments arranged by size: 2301 2083 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2900 2988 4695 4780 5851 2900 Size: 88 1707 85 1071 3683 Fragments arranged by size: 3683 1707 1071 88 85 BsmI GAATG_Cn' Cuts at: 2465 2841 3101 4145 4238 2465 Size: 376 260 1044 93 4861 Fragments arranged by size: 4861 1044 376 260 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2364 2435 2900 2988 3953 4695 4780 Size: 399 574 71 465 88 965 742 85 Cuts at: 4780 5851 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 965 742 574 465 399 88 85 71 BspEI T'CCGG_A Cuts at: 6297 6297 Size: 6634 BspHI T'CATG_A Cuts at: 4523 6253 4523 Size: 1730 4904 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2381 3989 844 Size: 1537 1608 3489 Fragments arranged by size: 3489 1608 1537 BsrBI CCG'CTC Cuts at: 1394 1823 4521 1394 Size: 429 2698 3507 Fragments arranged by size: 3507 2698 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5129 5303 65 Size: 1183 3881 174 1396 Fragments arranged by size: 3881 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5278 5465 1700 Size: 110 3468 187 2869 Fragments arranged by size: 3468 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2334 96 Size: 2238 4396 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6634 BssSI C'ACGA_G Cuts at: 4694 5706 4694 Size: 1012 5622 BstAPI GCAn_nnn'nTGC Cuts at: 794 2373 794 Size: 1579 5055 BstBI TT'CG_AA Cuts at: 5438 5438 Size: 6634 BstEII G'GTnAC_C Cuts at: 1184 3259 1184 Size: 2075 4559 BstYI r'GATC_y Cuts at: 1972 2100 3648 3964 4331 4716 4733 6185 6271 Size: 128 1548 316 367 385 17 1452 86 Cuts at: 6271 6283 6629 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1548 1452 385 367 346 316 128 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2532 2532 Size: 6634 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6082 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2986 487 Size: 683 56 1760 4135 Fragments arranged by size: 4135 1760 683 56 BtsI GCAGTG_nn' Cuts at: 2876 4150 4944 4964 2876 Size: 1274 794 20 4546 Fragments arranged by size: 4546 1274 794 20 ClaI AT'CG_AT Cuts at: 1554 3225 1554 Size: 1671 4963 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6599 DraI TTT'AAA Cuts at: 3938 3938 Size: 6634 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5641 809 Size: 507 4325 1802 Fragments arranged by size: 4325 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3667 4081 4970 Size: 54 1425 50 275 18 1837 414 889 Cuts at: 4970 6599 8 Size: 1629 43 Fragments arranged by size: 1837 1629 1425 889 414 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4081 1537 Size: 275 2269 4090 Fragments arranged by size: 4090 2269 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2386 3323 4564 1353 Size: 1033 937 1241 3423 Fragments arranged by size: 3423 1241 1033 937 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5221 5595 5741 387 Size: 1064 138 3632 374 146 1280 Fragments arranged by size: 3632 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2986 2986 Size: 6634 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 2472 2721 3002 3066 3134 3200 Size: 393 654 186 249 281 64 68 66 Cuts at: 3200 4697 5294 6081 6349 1239 Size: 1497 597 787 268 1524 Fragments arranged by size: 1524 1497 787 654 597 393 281 268 249 186 68 66 64 EcoNI CCTnn'n_nnAGG Cuts at: 2868 2868 Size: 6634 EcoO109I rG'GnC_Cy Cuts at: 2809 2809 Size: 6634 EcoRI G'AATT_C Cuts at: 2166 3943 4321 2166 Size: 1777 378 4479 Fragments arranged by size: 4479 1777 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2585 2585 Size: 6634 FspI TGC'GCA Cuts at: 5140 5140 Size: 6634 HaeII r_GCGC'y Cuts at: 1678 5781 1678 Size: 4103 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2962 2994 3725 3757 3785 Size: 32 135 32 1390 32 731 32 28 Cuts at: 3785 3817 5275 5307 5349 5381 1373 Size: 32 1458 32 42 32 2626 Fragments arranged by size: 2626 1458 1390 731 135 42 32 32 32 32 32 32 32 28 HincII GTy'rAC Cuts at: 669 1657 1938 2215 3978 4224 669 Size: 988 281 277 1763 246 3079 Fragments arranged by size: 3079 1763 988 281 277 246 HindIII A'AGCT_T Cuts at: 748 3107 748 Size: 2359 4275 HpaI GTT'AAC Cuts at: 2215 4224 2215 Size: 2009 4625 KpnI G_GTAC'C Cuts at: 3959 3959 Size: 6634 MfeI C'AATT_G Cuts at: 3170 4233 3170 Size: 1063 5571 MluI A'CGCG_T Cuts at: 6510 6510 Size: 6634 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5748 5932 1305 Size: 4443 184 2007 Fragments arranged by size: 4443 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3669 10 Size: 54 1425 343 1837 2975 Fragments arranged by size: 2975 1837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2330 4592 4951 5110 518 Size: 648 1164 2262 359 159 2042 Fragments arranged by size: 2262 2042 1164 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2922 2956 4050 4727 5875 6120 1606 Size: 1316 34 1094 677 1148 245 2120 Fragments arranged by size: 2120 1316 1148 1094 677 245 34 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6082 NdeI CA'TA_TG Cuts at: 387 387 Size: 6634 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6634 NotI GC'GGCC_GC Cuts at: 4081 4081 Size: 6634 NspI r_CATG'y Cuts at: 1132 1229 3992 5537 1132 Size: 97 2763 1545 2229 Fragments arranged by size: 2763 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6634 PciI A'CATG_T Cuts at: 5533 5533 Size: 6634 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6634 PfoI T'CCnGG_A Cuts at: 2263 2263 Size: 6634 PmeI GTTT'AAAC Cuts at: 3938 3938 Size: 6634 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2110 2142 4703 4735 6216 6248 2110 Size: 32 2561 32 1481 32 2496 Fragments arranged by size: 2561 2496 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2809 2809 Size: 6634 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6634 PsiI TTA'TAA Cuts at: 2283 3492 4204 2283 Size: 1209 712 4713 Fragments arranged by size: 4713 1209 712 PstI C_TGCA'G Cuts at: 830 3986 830 Size: 3156 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6634 PvuII CAG'CTG Cuts at: 2922 2922 Size: 6634 SacI G_AGCT'C Cuts at: 2988 2988 Size: 6634 SalI G'TCGA_C Cuts at: 1936 3976 1936 Size: 2040 4594 SapI GCTCTTCn'nnn_ Cuts at: 3323 3323 Size: 6634 SbfI CC_TGCA'GG Cuts at: 3986 3986 Size: 6634 ScaI AGT'ACT Cuts at: 3624 4882 6594 3624 Size: 1258 1712 3664 Fragments arranged by size: 3664 1712 1258 SfcI C'TryA_G Cuts at: 826 1045 2908 3982 5117 5798 5989 826 Size: 219 1863 1074 1135 681 191 1471 Fragments arranged by size: 1863 1471 1135 1074 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6634 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6634 SmaI CCC'GGG Cuts at: 1872 3961 1872 Size: 2089 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4738 5639 5901 820 Size: 197 926 2795 901 262 1553 Fragments arranged by size: 2795 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4425 493 Size: 3932 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6634 SphI G_CATG'C Cuts at: 1132 3992 1132 Size: 2860 3774 SspI AAT'ATT Cuts at: 5 52 4558 5 Size: 47 4506 2081 Fragments arranged by size: 4506 2081 47 StuI AGG'CCT Cuts at: 1517 2698 3684 1517 Size: 1181 986 4467 Fragments arranged by size: 4467 1181 986 StyI C'CwwG_G Cuts at: 513 1065 2081 2737 3628 4073 513 Size: 552 1016 656 891 445 3074 Fragments arranged by size: 3074 1016 891 656 552 445 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2562 4661 4678 4831 5016 5461 325 Size: 1255 982 2099 17 153 185 445 1498 Fragments arranged by size: 2099 1498 1255 982 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2334 3481 3622 Size: 274 80 33 51 1443 357 1147 141 Cuts at: 3622 4880 6592 96 Size: 1258 1712 138 Fragments arranged by size: 1712 1443 1258 1147 357 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3575 3607 6594 6626 3575 Size: 32 2987 32 3583 Fragments arranged by size: 3583 2987 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3737 1316 Size: 2421 4213 XbaI T'CTAG_A Cuts at: 2636 3547 3889 3970 2636 Size: 911 342 81 5300 Fragments arranged by size: 5300 911 342 81 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6634 XmaI C'CCGG_G Cuts at: 1870 3959 1870 Size: 2089 4545 XmnI GAAnn'nnTTC Cuts at: 2076 2568 3777 4763 2076 Size: 492 1209 986 3947 Fragments arranged by size: 3947 1209 986 492 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4442 276 Size: 53 83 186 936 2908 2468 Fragments arranged by size: 2908 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI