Restriction map for
pFN21AA1460
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# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII AccII AflII AgeI AhdI
Aor51HI ApaI AscI AspEI AvrII
BbeI BfrI BlnI BlpI Bpu1102I
BsiWI BsmBI BsrBI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CelII Eam1105I Eco47III EcoO65I EcoRV
EcoT22I FseI HaeII KasI KspI
MfeI MluI MunI MvnI NarI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PstI
PvuI SacI SacII SapI SbfI
SexAI SfoI SfuI SgrAI SnaBI
Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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Acc65I 1252 GGTACC G^GTACC
AccIII 738 TCCGGA T^CCGGA
AclI 5044 AACGTT AA^CGTT
Alw44I 3914 GTGCAC G^TGCAC
ApaLI 3914 GTGCAC G^TGCAC
Asp700I 4449 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1252 GGTACC G^GTACC
AviII 5390 TGCGCA TGC^GCA
BamHI 610 GGATCC G^GATCC
BcgI 4362 GCAACAATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 4235 AGATCT A^GATCT
BseAI 738 TCCGGA T^CCGGA
Bsp1407I 3663 TGTACA T^GTACA
BspDI 5374 ATCGAT AT^CGAT
BspEI 738 TCCGGA T^CCGGA
BspHI 5142 TCATGA T^CATGA
BspMI 2361 ACCTGC ACCTGC(4/8)
BsrGI 3663 TGTACA T^GTACA
BssSI 398 CTCGTG CACGAG(-5/-1)
ClaI 5374 ATCGAT AT^CGAT
CpoI 5680 CGGACCG CG^G[AT]CCG
DraI 3489 TTTAAA TTT^AAA
DraIII 5394 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 4309 CGGCCG C^GGCCG
EclXI 4309 CGGCCG C^GGCCG
Eco52I 4309 CGGCCG C^GGCCG
EcoNI 2896 CCTGGTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 5390 TGCGCA TGC^GCA
HindIII 3895 AAGCTT A^AGCTT
HpaI 1693 GTTAAC GTT^AAC
KpnI 1252 GGTACC GGTAC^C
MroI 738 TCCGGA T^CCGGA
NaeI 5639 GCCGGC GCC^GGC
NgoMIV 5639 GCCGGC G^CCGGC
NheI 1090 GCTAGC G^CTAGC
Psp1406I 5044 AACGTT AA^CGTT
RcaI 5142 TCATGA T^CATGA
RsrII 5680 CGGACCG CG^G[AT]CCG
SalI 4331 GTCGAC G^TCGAC
SfiI 1606 GGCCCTAATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 4031 CCCGGG CCC^GGG
SspBI 3663 TGTACA T^GTACA
XmaCI 4031 CCCGGG C^CCGGG
XmaI 4031 CCCGGG C^CCGGG
XmnI 4449 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 31 enzymes) #
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AccI 4331 GTCGAC GT^[AC][GT]AC
5232 GTCTAC
AcyI 2039 GACGCC G[GA]^CG[CT]C
5284 GACGCC
AlwNI 722 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
5590 CAGTCTCTG
AocI 4117 CCTCAGG CC^T[ACGT]AGG
4953 CCTCAGG
AseI 1171 ATTAAT AT^TAAT
4927 ATTAAT
AsnI 1171 ATTAAT AT^TAAT
4927 ATTAAT
AspI 4462 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5103 GACATGGTC
BbsI 645 GTCTTC GAAGAC(2/6)
3804 GTCTTC
BciVI 2202 GGATAC GTATCC(6/5)
393 GTATCC
BclI 2094 TGATCA T^GATCA
5420 TGATCA
BpuAI 645 GTCTTC GAAGAC(2/6)
3804 GTCTTC
BsaHI 2039 GACGCC G[GA]^CG[CT]C
5284 GACGCC
BsiEI 3761 CGGTCG CG[GA][CT]^CG
4309 CGGCCG
BsrFI 4307 ACCGGC [GA]^CCGG[CT]
5639 GCCGGC
BstAPI 4353 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5393 GCACTCTGTGC
Bsu36I 4117 CCTCAGG CC^T[ACGT]AGG
4953 CCTCAGG
Cfr10I 4307 ACCGGC [GA]^CCGG[CT]
5639 GCCGGC
DrdI 4885 GACCCTTACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
5207 GACTGACTGGTC
Eco81I 4117 CCTCAGG CC^T[ACGT]AGG
4953 CCTCAGG
EcoRI 1083 GAATTC G^AATTC
3969 GAATTC
FbaI 2094 TGATCA T^GATCA
5420 TGATCA
HgaI 2039 GACGC GACGC(5/10)
5284 GACGC
Hin1I 2039 GACGCC G[GA]^CG[CT]C
5284 GACGCC
PflFI 4462 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5103 GACATGGTC
PshAI 638 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3256 GACAGTGGTC
PshBI 1171 ATTAAT AT^TAAT
4927 ATTAAT
SpeI 2677 ACTAGT A^CTAGT
5008 ACTAGT
SphI 4037 GCATGC GCATG^C
5402 GCATGC
StuI 380 AGGCCT AGG^CCT
2252 AGGCCT
Tth111I 4462 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
5103 GACATGGTC
XcmI 3941 CCATAGCAGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
5299 CCAGGTTCCAGCTGG
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# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AspHI AvaI AvaII
BalI BanI BanII BbrPI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BsaAI BsaBI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspLU11I BsrDI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PmaCI
PmlI PpuMI PvuII RsaI Sau3AI
Sau96I ScaI ScrFI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII