(Circular) (Six-base) MAPSORT of: pFN21AA1460.seq Check: 8802 from: 1 to: 10591 pFN21AA1460 10591 bp With 182 enzymes: SgfI * January 31, 2011 17:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8401 278 Size: 53 83 186 936 6865 2468 Fragments arranged by size: 6865 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6337 7238 7934 1937 Size: 4400 901 696 4594 Fragments arranged by size: 4594 4400 901 696 Acc65I G'GTAC_C Cuts at: 3257 7912 3257 Size: 4655 5936 AclI AA'CG_TT Cuts at: 1563 7050 8718 9091 1563 Size: 5487 1668 373 3063 Fragments arranged by size: 5487 3063 1668 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2595 2739 2805 3257 3312 5004 5223 6164 Size: 963 144 66 452 55 1692 219 941 Cuts at: 6164 7065 8654 10038 10306 1632 Size: 901 1589 1384 268 1917 Fragments arranged by size: 1917 1692 1589 1384 963 941 901 452 268 219 144 66 55 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10394 AflIII A'CryG_T Cuts at: 1203 2972 4150 5064 7515 9490 10467 1203 Size: 1769 1178 914 2451 1975 977 1327 Fragments arranged by size: 2451 1975 1769 1327 1178 977 914 AgeI A'CCGG_T Cuts at: 9422 9422 Size: 10591 AhdI GACnn_n'nnGTC Cuts at: 9320 9320 Size: 10591 AleI CACnn'nnGTG Cuts at: 1166 5222 7518 7869 1166 Size: 4056 2296 351 3888 Fragments arranged by size: 4056 3888 2296 351 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4215 1184 Size: 3031 7560 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3151 3183 6200 6232 3151 Size: 32 3017 32 7510 Fragments arranged by size: 7510 3017 32 32 AlwNI CAG_nnn'CTG Cuts at: 2732 7600 8334 9906 2732 Size: 4868 734 1572 3417 Fragments arranged by size: 4868 3417 1572 734 ApaLI G'TGCA_C Cuts at: 5919 8648 9804 5919 Size: 2729 1156 6706 Fragments arranged by size: 6706 2729 1156 ApoI r'AATT_y Cuts at: 1493 2629 3088 3781 5974 6836 7282 7900 8126 Size: 1136 459 693 2193 862 446 618 226 Cuts at: 8126 8278 10387 1493 Size: 152 2109 1697 Fragments arranged by size: 2193 2109 1697 1136 862 693 618 459 446 226 152 AseI AT'TA_AT Cuts at: 160 1032 3177 6933 9145 160 Size: 872 2145 3756 2212 1606 Fragments arranged by size: 3756 2212 2145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10591 AvaI C'yCGr_G Cuts at: 1870 1943 6036 7628 7815 7916 1870 Size: 73 4093 1592 187 101 4545 Fragments arranged by size: 4545 4093 1592 187 101 73 BamHI G'GATC_C Cuts at: 2615 7921 8288 2615 Size: 5306 367 4918 Fragments arranged by size: 5306 4918 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3107 3257 3572 7912 9367 Size: 325 217 627 1320 150 315 4340 1455 Cuts at: 9367 618 Size: 1842 Fragments arranged by size: 4340 1842 1455 1320 627 325 315 217 150 BanII G_rGCy'C Cuts at: 1391 5193 5321 7655 7910 1391 Size: 3802 128 2334 255 4072 Fragments arranged by size: 4072 3802 2334 255 128 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2643 5802 928 Size: 971 744 3159 5717 Fragments arranged by size: 5717 3159 971 744 BbvCI CC'TCA_GC Cuts at: 2202 2725 7058 2202 Size: 523 4333 5735 Fragments arranged by size: 5735 4333 523 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6356 6390 8782 8816 1903 Size: 34 4419 34 2392 34 3678 Fragments arranged by size: 4419 3678 2392 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2409 4201 8483 9693 2409 Size: 1792 4282 1210 3307 Fragments arranged by size: 4282 3307 1792 1210 BclI T'GATC_A Cuts at: 1548 4099 7425 1548 Size: 2551 3326 4714 Fragments arranged by size: 4714 3326 2551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3618 5421 7650 Size: 107 122 71 631 605 1946 1803 2229 Cuts at: 7650 9202 136 Size: 1552 1525 Fragments arranged by size: 2229 1946 1803 1552 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6240 10586 6240 Size: 4346 6245 BlpI GC'TnA_GC Cuts at: 8008 8008 Size: 10591 Bme1580I G_kGCm'C Cuts at: 1790 3044 3686 5923 8652 9808 1790 Size: 1254 642 2237 2729 1156 2573 Fragments arranged by size: 2729 2573 2237 1254 1156 642 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 10591 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2181 2943 4074 Size: 416 53 83 399 24 764 762 1131 Cuts at: 4074 4582 5994 6241 7535 8322 9280 442 Size: 508 1412 247 1294 787 958 1753 Fragments arranged by size: 1753 1412 1294 1131 958 787 764 762 508 416 399 247 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3099 1056 Size: 2043 8548 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5503 7345 5503 Size: 1842 8749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2767 3615 3807 4818 5196 5305 5715 6039 Size: 1528 848 192 1011 378 109 410 324 Cuts at: 6039 6649 6747 7663 9251 1239 Size: 610 98 916 1588 2579 Fragments arranged by size: 2579 1588 1528 1011 916 848 610 410 378 324 192 109 98 Bpu10I CC'TnA_GC Cuts at: 2202 2725 3524 4627 6492 7058 7330 2202 Size: 523 799 1103 1865 566 272 5463 Fragments arranged by size: 5463 1865 1103 799 566 523 272 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4070 5460 6081 8716 9581 9879 4070 Size: 1390 621 2635 865 298 4782 Fragments arranged by size: 4782 2635 1390 865 621 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2986 4767 7119 7731 9254 882 Size: 617 1487 1781 2352 612 1523 2219 Fragments arranged by size: 2352 2219 1781 1523 1487 617 612 BsaAI yAC'GTr Cuts at: 493 1204 4151 5313 6791 7242 8382 493 Size: 711 2947 1162 1478 451 1140 2702 Fragments arranged by size: 2947 2702 1478 1162 1140 711 451 BsaBI GATnn'nnATC Cuts at: 3928 5757 7695 10585 3928 Size: 1829 1938 2890 3934 Fragments arranged by size: 3934 2890 1938 1829 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4045 7290 8398 8780 Size: 53 83 186 936 2512 3245 1108 382 Cuts at: 8780 9415 275 Size: 635 1451 Fragments arranged by size: 3245 2512 1451 1108 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2743 3404 4658 9024 9422 9696 9843 10254 2743 Size: 661 1254 4366 398 274 147 411 3080 Fragments arranged by size: 4366 3080 1254 661 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3151 3181 3705 3735 3996 Size: 30 165 30 1748 30 524 30 261 Cuts at: 3996 4026 7099 7129 7594 7624 7661 7691 1178 Size: 30 3073 30 465 30 37 30 4078 Fragments arranged by size: 4078 3073 1748 524 465 261 165 37 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4490 6096 7580 1186 Size: 3304 1606 1484 4197 Fragments arranged by size: 4197 3304 1606 1484 BseYI C'CCAG_C Cuts at: 2634 3842 4915 4936 4962 5181 5270 5274 5424 Size: 1208 1073 21 26 219 89 4 150 Cuts at: 5424 5956 6219 7314 7648 7712 9794 10290 2634 Size: 532 263 1095 334 64 2082 496 2935 Fragments arranged by size: 2935 2082 1208 1095 1073 532 496 334 263 219 150 89 64 26 21 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2591 3208 3434 3756 3801 6371 7496 Size: 573 855 617 226 322 45 2570 1125 Cuts at: 7496 1163 Size: 4258 Fragments arranged by size: 4258 2570 1125 855 617 573 322 226 45 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5769 6317 8041 8802 9830 1540 Size: 275 186 3768 548 1724 761 1028 2301 Fragments arranged by size: 3768 2301 1724 1028 761 548 275 186 BsiHKAI G_wGCw'C Cuts at: 3464 5923 6097 8652 8737 9808 3464 Size: 2459 174 2555 85 1071 4247 Fragments arranged by size: 4247 2555 2459 1071 174 85 BsmI GAATG_Cn' Cuts at: 2689 3037 3649 3877 8102 8195 2689 Size: 348 612 228 4225 93 5085 Fragments arranged by size: 5085 4225 612 348 228 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3044 3464 3686 5193 5321 5923 6097 Size: 399 1254 420 222 1507 128 602 174 Cuts at: 6097 7655 7910 8652 8737 9808 1391 Size: 1558 255 742 85 1071 2174 Fragments arranged by size: 2174 1558 1507 1254 1071 742 602 420 399 255 222 174 128 85 BspEI T'CCGG_A Cuts at: 2743 10254 2743 Size: 7511 3080 BspHI T'CATG_A Cuts at: 7147 8480 10210 7147 Size: 1333 1730 7528 Fragments arranged by size: 7528 1730 1333 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4375 7946 844 Size: 3531 3571 3489 Fragments arranged by size: 3571 3531 3489 BsrBI CCG'CTC Cuts at: 1394 1823 8478 1394 Size: 429 6655 3507 Fragments arranged by size: 6655 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3535 3907 5286 5399 6137 9086 9260 Size: 1183 2287 372 1379 113 738 2949 174 Cuts at: 9260 65 Size: 1396 Fragments arranged by size: 2949 2287 1396 1379 1183 738 372 174 113 BsrFI r'CCGG_y Cuts at: 1700 1810 6312 7644 9235 9422 1700 Size: 110 4502 1332 1591 187 2869 Fragments arranged by size: 4502 2869 1591 1332 187 110 BsrGI T'GTAC_A Cuts at: 96 5668 96 Size: 5572 5019 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10591 BssSI C'ACGA_G Cuts at: 2403 8651 9663 2403 Size: 6248 1012 3331 Fragments arranged by size: 6248 3331 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 6364 7404 794 Size: 5570 1040 3981 Fragments arranged by size: 5570 3981 1040 BstBI TT'CG_AA Cuts at: 9395 9395 Size: 10591 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10591 BstXI CCAn_nnnn'nTGG Cuts at: 3629 5224 5939 7871 3629 Size: 1595 715 1932 6349 Fragments arranged by size: 6349 1932 1595 715 BstYI r'GATC_y Cuts at: 1972 2492 2615 4144 4865 5032 6240 7921 8288 Size: 520 123 1529 721 167 1208 1681 367 Cuts at: 8288 8673 8690 10142 10228 10240 10586 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1681 1529 1452 1208 721 520 385 367 346 167 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 6123 6959 6123 Size: 836 9755 BtgI C'CryG_G Cuts at: 513 1065 2920 2939 2953 3103 3253 5814 6854 Size: 552 1855 19 14 150 150 2561 1040 Cuts at: 6854 7887 513 Size: 1033 3217 Fragments arranged by size: 3217 2561 1855 1040 1033 552 150 150 19 14 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4035 487 Size: 683 56 2809 7043 Fragments arranged by size: 7043 2809 683 56 BtsI GCAGTG_nn' Cuts at: 2536 2743 3991 5176 6823 6916 7185 8107 8901 Size: 207 1248 1185 1647 93 269 922 794 Cuts at: 8901 8921 2536 Size: 20 4206 Fragments arranged by size: 4206 1647 1248 1185 922 794 269 207 93 20 ClaI AT'CG_AT Cuts at: 1554 7380 1554 Size: 5826 4765 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2370 2405 3697 3732 529 Size: 35 1806 35 1292 35 7388 Fragments arranged by size: 7388 1806 1292 35 35 35 DraI TTT'AAA Cuts at: 5496 7895 5496 Size: 2399 8192 DraIII CAC_nnn'GTG Cuts at: 7404 7404 Size: 10591 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6896 7218 9598 809 Size: 507 5580 322 2380 1802 Fragments arranged by size: 5580 2380 1802 507 322 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3113 3619 5501 Size: 54 1425 50 275 18 1283 506 1882 Cuts at: 5501 6314 6785 6827 8038 8927 10556 8 Size: 813 471 42 1211 889 1629 43 Fragments arranged by size: 1882 1629 1425 1283 1211 889 813 506 471 275 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 6314 8038 1537 Size: 275 4502 1724 4090 Fragments arranged by size: 4502 4090 1724 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3793 3936 5424 5430 6699 7479 7557 8521 Size: 2440 143 1488 6 1269 780 78 964 Cuts at: 8521 1353 Size: 3423 Fragments arranged by size: 3423 2440 1488 1269 964 780 143 78 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9178 9552 9698 387 Size: 1064 138 7589 374 146 1280 Fragments arranged by size: 7589 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2595 2739 2767 2805 3257 3312 3615 Size: 393 963 144 28 38 452 55 303 Cuts at: 3615 3807 4818 5004 5196 5223 5305 5715 6039 Size: 192 1011 186 192 27 82 410 324 Cuts at: 6039 6164 6649 6747 7065 7663 8654 9251 10038 Size: 125 485 98 318 598 991 597 787 Cuts at: 10038 10306 1239 Size: 268 1524 Fragments arranged by size: 1524 1011 991 963 787 598 597 485 452 410 393 324 318 303 268 192 192 186 144 125 98 82 55 38 28 27 EcoNI CCTnn'n_nnAGG Cuts at: 4905 4905 Size: 10591 EcoO109I rG'GnC_Cy Cuts at: 2642 3149 3447 3611 4922 5777 5935 7162 7764 Size: 507 298 164 1311 855 158 1227 602 Cuts at: 7764 7793 2642 Size: 29 5440 Fragments arranged by size: 5440 1311 1227 855 602 507 298 164 158 29 EcoRI G'AATT_C Cuts at: 3088 5974 7900 8278 3088 Size: 2886 1926 378 5401 Fragments arranged by size: 5401 2886 1926 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2550 2867 6318 7249 2550 Size: 317 3451 931 5892 Fragments arranged by size: 5892 3451 931 317 FspI TGC'GCA Cuts at: 7397 9097 7397 Size: 1700 8891 HaeII r_GCGC'y Cuts at: 1678 9738 1678 Size: 8060 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2765 2797 3472 3504 4322 Size: 32 135 32 1193 32 675 32 818 Cuts at: 4322 4354 4958 4990 6584 6616 6647 6679 7099 Size: 32 604 32 1594 32 31 32 420 Cuts at: 7099 7131 7594 7626 9232 9264 9306 9338 1373 Size: 32 463 32 1606 32 42 32 2626 Fragments arranged by size: 2626 1606 1594 1193 818 675 604 463 420 135 42 32 32 32 32 32 32 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 3329 3700 6338 6380 6952 7861 Size: 988 281 1391 371 2638 42 572 909 Cuts at: 7861 7935 8181 669 Size: 74 246 3079 Fragments arranged by size: 3079 2638 1391 988 909 572 371 281 246 74 42 HindIII A'AGCT_T Cuts at: 748 5900 748 Size: 5152 5439 HpaI GTT'AAC Cuts at: 3700 8181 3700 Size: 4481 6110 KpnI G_GTAC'C Cuts at: 3261 7916 3261 Size: 4655 5936 MfeI C'AATT_G Cuts at: 8190 8190 Size: 10591 MluI A'CGCG_T Cuts at: 10467 10467 Size: 10591 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2685 4185 4643 4843 5995 7065 9705 9889 Size: 1380 1500 458 200 1152 1070 2640 184 Cuts at: 9889 1305 Size: 2007 Fragments arranged by size: 2640 2007 1500 1380 1152 1070 458 200 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3115 3621 5503 6787 6829 Size: 54 1425 343 1283 506 1882 1284 42 Cuts at: 6829 10 Size: 3772 Fragments arranged by size: 3772 1882 1425 1284 1283 506 343 54 42 MslI CAynn'nnrTG Cuts at: 518 1166 2813 3350 3882 4602 4729 5222 5311 Size: 648 1647 537 532 720 127 493 89 Cuts at: 5311 6638 7455 7518 7704 7869 8549 8908 9067 Size: 1327 817 63 186 165 680 359 159 Cuts at: 9067 518 Size: 2042 Fragments arranged by size: 2042 1647 1327 817 720 680 648 537 532 493 359 186 165 159 127 89 63 MspA1I CmG'CkG Cuts at: 1606 2638 5128 5274 7314 7423 8007 8684 9832 Size: 1032 2490 146 2040 109 584 677 1148 Cuts at: 9832 10077 1606 Size: 245 2120 Fragments arranged by size: 2490 2120 2040 1148 1032 677 584 245 146 109 NaeI GCC'GGC Cuts at: 7646 7646 Size: 10591 NcoI C'CATG_G Cuts at: 513 1065 2920 2939 2953 3103 3253 5814 6854 Size: 552 1855 19 14 150 150 2561 1040 Cuts at: 6854 7887 513 Size: 1033 3217 Fragments arranged by size: 3217 2561 1855 1040 1033 552 150 150 19 14 NdeI CA'TA_TG Cuts at: 387 387 Size: 10591 NgoMIV G'CCGG_C Cuts at: 7644 7644 Size: 10591 NheI G'CTAG_C Cuts at: 1052 3095 1052 Size: 2043 8548 NotI GC'GGCC_GC Cuts at: 8038 8038 Size: 10591 NspI r_CATG'y Cuts at: 1132 1229 2976 4302 5068 5875 6046 7411 7519 Size: 97 1747 1326 766 807 171 1365 108 Cuts at: 7519 7949 9494 1132 Size: 430 1545 2229 Fragments arranged by size: 2229 1747 1545 1365 1326 807 766 430 171 108 97 PasI CC'CwG_GG Cuts at: 1348 4762 5193 1348 Size: 3414 431 6746 Fragments arranged by size: 6746 3414 431 PciI A'CATG_T Cuts at: 2972 5064 7515 9490 2972 Size: 2092 2451 1975 4073 Fragments arranged by size: 4073 2451 2092 1975 PflMI CCAn_nnn'nTGG Cuts at: 1108 4962 5405 5424 6182 1108 Size: 3854 443 19 758 5517 Fragments arranged by size: 5517 3854 758 443 19 PfoI T'CCnGG_A Cuts at: 7207 7207 Size: 10591 PmeI GTTT'AAAC Cuts at: 7895 7895 Size: 10591 PmlI CAC'GTG Cuts at: 4151 5313 6791 4151 Size: 1162 1478 7951 Fragments arranged by size: 7951 1478 1162 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3151 3183 3229 3261 8660 8692 10173 10205 3151 Size: 32 46 32 5399 32 1481 32 3537 Fragments arranged by size: 5399 3537 1481 46 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2642 4922 7162 7764 7793 2642 Size: 2280 2240 602 29 5440 Fragments arranged by size: 5440 2280 2240 602 29 PshAI GACnn'nnGTC Cuts at: 1613 2647 5265 1613 Size: 1034 2618 6939 Fragments arranged by size: 6939 2618 1034 PsiI TTA'TAA Cuts at: 8161 8161 Size: 10591 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4669 4701 4669 Size: 32 10559 PstI C_TGCA'G Cuts at: 830 7943 830 Size: 7113 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10591 PvuII CAG'CTG Cuts at: 5128 5274 7314 5128 Size: 146 2040 8405 Fragments arranged by size: 8405 2040 146 RsrII CG'GwC_CG Cuts at: 7686 7686 Size: 10591 SalI G'TCGA_C Cuts at: 1936 6336 7933 1936 Size: 4400 1597 4594 Fragments arranged by size: 4594 4400 1597 SanDI GG'GwC_CC Cuts at: 7764 7793 7764 Size: 29 10562 SbfI CC_TGCA'GG Cuts at: 7943 7943 Size: 10591 ScaI AGT'ACT Cuts at: 2608 2932 3193 6745 8839 10551 2608 Size: 324 261 3552 2094 1712 2648 Fragments arranged by size: 3552 2648 2094 1712 324 261 SfcI C'TryA_G Cuts at: 826 1045 2268 3153 3195 4434 4887 6540 7471 Size: 219 1223 885 42 1239 453 1653 931 Cuts at: 7471 7939 9074 9755 9946 826 Size: 468 1135 681 191 1471 Fragments arranged by size: 1653 1471 1239 1223 1135 931 885 681 468 453 219 191 42 SfiI GGCCn_nnn'nGGCC Cuts at: 3618 3618 Size: 10591 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10591 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10591 SmaI CCC'GGG Cuts at: 1872 6038 7918 1872 Size: 4166 1880 4545 Fragments arranged by size: 4545 4166 1880 SmlI C'TyrA_G Cuts at: 820 1017 1943 4049 5475 6096 8695 9596 9858 Size: 197 926 2106 1426 621 2599 901 262 Cuts at: 9858 820 Size: 1553 Fragments arranged by size: 2599 2106 1553 1426 926 901 621 262 197 SnaBI TAC'GTA Cuts at: 493 8382 493 Size: 7889 2702 SpeI A'CTAG_T Cuts at: 152 4682 7013 152 Size: 4530 2331 3730 Fragments arranged by size: 4530 3730 2331 SphI G_CATG'C Cuts at: 1132 6046 7411 7949 1132 Size: 4914 1365 538 3774 Fragments arranged by size: 4914 3774 1365 538 SspI AAT'ATT Cuts at: 5 52 3181 3340 5746 8515 5 Size: 47 3129 159 2406 2769 2081 Fragments arranged by size: 3129 2769 2406 2081 159 47 StuI AGG'CCT Cuts at: 1517 2387 4259 1517 Size: 870 1872 7849 Fragments arranged by size: 7849 1872 870 StyI C'CwwG_G Cuts at: 513 1065 2488 2920 2939 2953 3103 3253 4543 Size: 552 1423 432 19 14 150 150 1290 Cuts at: 4543 5814 5938 6854 7887 8030 513 Size: 1271 124 916 1033 143 3074 Fragments arranged by size: 3074 1423 1290 1271 1033 916 552 432 150 150 143 124 19 14 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 7703 7859 8618 8635 8788 8973 9418 Size: 1255 6123 156 759 17 153 185 445 Cuts at: 9418 325 Size: 1498 Fragments arranged by size: 6123 1498 1255 759 445 185 156 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2606 2930 3191 Size: 274 80 33 51 1443 629 324 261 Cuts at: 3191 3752 4028 5668 6743 7064 7522 8837 10549 Size: 561 276 1640 1075 321 458 1315 1712 Cuts at: 10549 96 Size: 138 Fragments arranged by size: 1712 1640 1443 1315 1075 629 561 458 324 321 276 274 261 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2818 2850 3705 3737 3996 4028 7661 7693 10551 Size: 32 855 32 259 32 3633 32 2858 Cuts at: 10551 10583 2818 Size: 32 2826 Fragments arranged by size: 3633 2858 2826 855 259 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 6470 7111 1316 Size: 5154 641 4796 Fragments arranged by size: 5154 4796 641 XbaI T'CTAG_A Cuts at: 7927 7927 Size: 10591 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 5953 7311 2011 Size: 3942 1358 5291 Fragments arranged by size: 5291 3942 1358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10591 XmaI C'CCGG_G Cuts at: 1870 6036 7916 1870 Size: 4166 1880 4545 Fragments arranged by size: 4545 4166 1880 XmnI GAAnn'nnTTC Cuts at: 6458 8720 6458 Size: 2262 8329 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8399 276 Size: 53 83 186 936 6865 2468 Fragments arranged by size: 6865 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII RsrII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI SacI SacII SapI SexAI SfoI SrfI SwaI