Restriction map for
pFN21AA1460
(Linear) (Six-base) MAPSORT of: KIAA1460.seq Check: 8440 from: 1 to: 5886
KIAA1460.orf
With 182 enzymes: SgfI *
March 2, 2010 14:24 ..
AccI GT'mk_AC
Cuts at: 0 4332 5233 5886
Size: 4332 901 653
Fragments arranged by size:
4332 901 653
Acc65I G'GTAC_C
Cuts at: 0 1252 5886
Size: 1252 4634
AclI AA'CG_TT
Cuts at: 0 5045 5886
Size: 5045 841
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 734 800 1252 1307 2999 3218 4159
Size: 590 144 66 452 55 1692 219 941
Cuts at: 4159 5060 5886
Size: 901 826
Fragments arranged by size:
1692 941 901 826 590 452 219 144
66 55
AflIII A'CryG_T
Cuts at: 0 967 2145 3059 5510 5886
Size: 967 1178 914 2451 376
Fragments arranged by size:
2451 1178 967 914 376
AleI CACnn'nnGTG
Cuts at: 0 3217 5513 5864 5886
Size: 3217 2296 351 22
Fragments arranged by size:
3217 2296 351 22
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2210 5886
Size: 2210 3676
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 4195 4227 5886
Size: 1146 32 3017 32 1659
Fragments arranged by size:
3017 1659 1146 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 727 5595 5886
Size: 727 4868 291
Fragments arranged by size:
4868 727 291
ApaLI G'TGCA_C
Cuts at: 0 3914 5886
Size: 3914 1972
ApoI r'AATT_y
Cuts at: 0 624 1083 1776 3969 4831 5277 5886
Size: 624 459 693 2193 862 446 609
Fragments arranged by size:
2193 862 693 624 609 459 446
AseI AT'TA_AT
Cuts at: 0 1172 4928 5886
Size: 1172 3756 958
Fragments arranged by size:
3756 1172 958
AvaI C'yCGr_G
Cuts at: 0 4031 5623 5810 5886
Size: 4031 1592 187 76
Fragments arranged by size:
4031 1592 187 76
BamHI G'GATC_C
Cuts at: 0 610 5886
Size: 610 5276
BanI G'GyrC_C
Cuts at: 0 1102 1252 1567 5886
Size: 1102 150 315 4319
Fragments arranged by size:
4319 1102 315 150
BanII G_rGCy'C
Cuts at: 0 3188 3316 5650 5886
Size: 3188 128 2334 236
Fragments arranged by size:
3188 2334 236 128
BbsI GAAGACnn'nnnn_
Cuts at: 0 638 3797 5886
Size: 638 3159 2089
Fragments arranged by size:
3159 2089 638
BbvCI CC'TCA_GC
Cuts at: 0 197 720 5053 5886
Size: 197 523 4333 833
Fragments arranged by size:
4333 833 523 197
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4351 4385 5886
Size: 4351 34 1501
Fragments arranged by size:
4351 1501 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 404 2196 5886
Size: 404 1792 3690
Fragments arranged by size:
3690 1792 404
BclI T'GATC_A
Cuts at: 0 2094 5420 5886
Size: 2094 3326 466
Fragments arranged by size:
3326 2094 466
BglI GCCn_nnn'nGGC
Cuts at: 0 1613 3416 5645 5886
Size: 1613 1803 2229 241
Fragments arranged by size:
2229 1803 1613 241
BglII A'GATC_T
Cuts at: 0 4235 5886
Size: 4235 1651
Bme1580I G_kGCm'C
Cuts at: 0 1039 1681 3918 5886
Size: 1039 642 2237 1968
Fragments arranged by size:
2237 1968 1039 642
BmrI ACTGGGnnnn_n'
Cuts at: 0 176 938 2069 2577 3989 4236 5530 5886
Size: 176 762 1131 508 1412 247 1294 356
Fragments arranged by size:
1412 1294 1131 762 508 356 247 176
BmtI G_CTAG'C
Cuts at: 0 1094 5886
Size: 1094 4792
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3498 5340 5886
Size: 3498 1842 546
Fragments arranged by size:
3498 1842 546
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1610 1802 2813 3191 3300 3710 4034
Size: 762 848 192 1011 378 109 410 324
Cuts at: 4034 4644 4742 5658 5886
Size: 610 98 916 228
Fragments arranged by size:
1011 916 848 762 610 410 378 324
228 192 109 98
Bpu10I CC'TnA_GC
Cuts at: 0 197 720 1519 2622 4487 5053 5325 5886
Size: 197 523 799 1103 1865 566 272 561
Fragments arranged by size:
1865 1103 799 566 561 523 272 197
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2065 3455 4076 5886
Size: 2065 1390 621 1810
Fragments arranged by size:
2065 1810 1390 621
BsaI GGTCTCn'nnnn_
Cuts at: 0 981 2762 5114 5726 5886
Size: 981 1781 2352 612 160
Fragments arranged by size:
2352 1781 981 612 160
BsaAI yAC'GTr
Cuts at: 0 2146 3308 4786 5237 5886
Size: 2146 1162 1478 451 649
Fragments arranged by size:
2146 1478 1162 649 451
BsaBI GATnn'nnATC
Cuts at: 0 1923 3752 5690 5886
Size: 1923 1829 1938 196
Fragments arranged by size:
1938 1923 1829 196
BsaHI Gr'CG_yC
Cuts at: 0 2040 5285 5886
Size: 2040 3245 601
Fragments arranged by size:
3245 2040 601
BsaWI w'CCGG_w
Cuts at: 0 738 1399 2653 5886
Size: 738 661 1254 3233
Fragments arranged by size:
3233 1254 738 661
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1146 1176 1700 1730 1991 2021 5094 5124
Size: 1146 30 524 30 261 30 3073 30
Cuts at: 5124 5589 5619 5656 5686 5886
Size: 465 30 37 30 200
Fragments arranged by size:
3073 1146 524 465 261 200 37 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2485 4091 5575 5886
Size: 2485 1606 1484 311
Fragments arranged by size:
2485 1606 1484 311
BseYI C'CCAG_C
Cuts at: 0 629 1837 2910 2931 2957 3176 3265 3269
Size: 629 1208 1073 21 26 219 89 4
Cuts at: 3269 3419 3951 4214 5309 5643 5707 5886
Size: 150 532 263 1095 334 64 179
Fragments arranged by size:
1208 1095 1073 629 532 334 263 219
179 150 89 64 26 21 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1203 1429 1751 1796 4366 5491 5886
Size: 586 617 226 322 45 2570 1125 395
Fragments arranged by size:
2570 1125 617 586 395 322 226 45
BsiEI CG_ry'CG
Cuts at: 0 3764 4312 5886
Size: 3764 548 1574
Fragments arranged by size:
3764 1574 548
BsiHKAI G_wGCw'C
Cuts at: 0 1459 3918 4092 5886
Size: 1459 2459 174 1794
Fragments arranged by size:
2459 1794 1459 174
BsmI GAATG_Cn'
Cuts at: 0 684 1032 1644 1872 5886
Size: 684 348 612 228 4014
Fragments arranged by size:
4014 684 612 348 228
Bsp1286I G_dGCh'C
Cuts at: 0 1039 1459 1681 3188 3316 3918 4092 5650
Size: 1039 420 222 1507 128 602 174 1558
Cuts at: 5650 5886
Size: 236
Fragments arranged by size:
1558 1507 1039 602 420 236 222 174
128
BspEI T'CCGG_A
Cuts at: 0 738 5886
Size: 738 5148
BspHI T'CATG_A
Cuts at: 0 5142 5886
Size: 5142 744
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2370 5886
Size: 2370 3516
BsrDI GCAATG_nn'
Cuts at: 0 1530 1902 3281 3394 4132 5886
Size: 1530 372 1379 113 738 1754
Fragments arranged by size:
1754 1530 1379 738 372 113
BsrFI r'CCGG_y
Cuts at: 0 4307 5639 5886
Size: 4307 1332 247
Fragments arranged by size:
4307 1332 247
BsrGI T'GTAC_A
Cuts at: 0 3663 5886
Size: 3663 2223
BssSI C'ACGA_G
Cuts at: 0 398 5886
Size: 398 5488
BstAPI GCAn_nnn'nTGC
Cuts at: 0 4359 5399 5886
Size: 4359 1040 487
Fragments arranged by size:
4359 1040 487
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1624 3219 3934 5866 5886
Size: 1624 1595 715 1932 20
Fragments arranged by size:
1932 1624 1595 715 20
BstYI r'GATC_y
Cuts at: 0 487 610 2139 2860 3027 4235 5886
Size: 487 123 1529 721 167 1208 1651
Fragments arranged by size:
1651 1529 1208 721 487 167 123
Bsu36I CC'TnA_GG
Cuts at: 0 4118 4954 5886
Size: 4118 836 932
Fragments arranged by size:
4118 932 836
BtgI C'CryG_G
Cuts at: 0 915 934 948 1098 1248 3809 4849 5886
Size: 915 19 14 150 150 2561 1040 1037
Fragments arranged by size:
2561 1040 1037 915 150 150 19 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2030 5886
Size: 2030 3856
BtsI GCAGTG_nn'
Cuts at: 0 531 738 1986 3171 4818 4911 5180 5886
Size: 531 207 1248 1185 1647 93 269 706
Fragments arranged by size:
1647 1248 1185 706 531 269 207 93
ClaI AT'CG_AT
Cuts at: 0 5375 5886
Size: 5375 511
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 365 400 1692 1727 5886
Size: 365 35 1292 35 4159
Fragments arranged by size:
4159 1292 365 35 35
DraI TTT'AAA
Cuts at: 0 3491 5886
Size: 3491 2395
DraIII CAC_nnn'GTG
Cuts at: 0 5399 5886
Size: 5399 487
DrdI GACnn_nn'nnGTC
Cuts at: 0 4891 5213 5886
Size: 4891 322 673
Fragments arranged by size:
4891 673 322
EaeI y'GGCC_r
Cuts at: 0 1108 1614 3496 4309 4780 4822 5886
Size: 1108 506 1882 813 471 42 1064
Fragments arranged by size:
1882 1108 1064 813 506 471 42
EagI C'GGCC_G
Cuts at: 0 4309 5886
Size: 4309 1577
EarI CTCTTCn'nnn_
Cuts at: 0 1788 1931 3419 3425 4694 5474 5552 5886
Size: 1788 143 1488 6 1269 780 78 334
Fragments arranged by size:
1788 1488 1269 780 334 143 78 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 590 734 762 800 1252 1307 1610 1802
Size: 590 144 28 38 452 55 303 192
Cuts at: 1802 2813 2999 3191 3218 3300 3710 4034 4159
Size: 1011 186 192 27 82 410 324 125
Cuts at: 4159 4644 4742 5060 5658 5886
Size: 485 98 318 598 228
Fragments arranged by size:
1011 598 590 485 452 410 324 318
303 228 192 192 186 144 125 98
82 55 38 28 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2900 5886
Size: 2900 2986
EcoO109I rG'GnC_Cy
Cuts at: 0 637 1144 1442 1606 2917 3772 3930 5157
Size: 637 507 298 164 1311 855 158 1227
Cuts at: 5157 5759 5788 5886
Size: 602 29 98
Fragments arranged by size:
1311 1227 855 637 602 507 298 164
158 98 29
EcoRI G'AATT_C
Cuts at: 0 1083 3969 5886
Size: 1083 2886 1917
Fragments arranged by size:
2886 1917 1083
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 545 862 4313 5244 5886
Size: 545 317 3451 931 642
Fragments arranged by size:
3451 931 642 545 317
FspI TGC'GCA
Cuts at: 0 5392 5886
Size: 5392 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1467 1499 2317 2349 2953 2985
Size: 760 32 675 32 818 32 604 32
Cuts at: 2985 4579 4611 4642 4674 5094 5126 5589 5621
Size: 1594 32 31 32 420 32 463 32
Cuts at: 5621 5886
Size: 265
Fragments arranged by size:
1594 818 760 675 604 463 420 265
32 32 32 32 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 1324 1695 4333 4375 4947 5856 5886
Size: 1324 371 2638 42 572 909 30
Fragments arranged by size:
2638 1324 909 572 371 42 30
HindIII A'AGCT_T
Cuts at: 0 3895 5886
Size: 3895 1991
HpaI GTT'AAC
Cuts at: 0 1695 5886
Size: 1695 4191
KpnI G_GTAC'C
Cuts at: 0 1256 5886
Size: 1256 4630
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 680 2180 2638 2838 3990 5060 5886
Size: 680 1500 458 200 1152 1070 826
Fragments arranged by size:
1500 1152 1070 826 680 458 200
MscI TGG'CCA
Cuts at: 0 1110 1616 3498 4782 4824 5886
Size: 1110 506 1882 1284 42 1062
Fragments arranged by size:
1882 1284 1110 1062 506 42
MslI CAynn'nnrTG
Cuts at: 0 808 1345 1877 2597 2724 3217 3306 4633
Size: 808 537 532 720 127 493 89 1327
Cuts at: 4633 5450 5513 5699 5864 5886
Size: 817 63 186 165 22
Fragments arranged by size:
1327 817 808 720 537 532 493 186
165 127 89 63 22
MspA1I CmG'CkG
Cuts at: 0 633 3123 3269 5309 5418 5886
Size: 633 2490 146 2040 109 468
Fragments arranged by size:
2490 2040 633 468 146 109
NaeI GCC'GGC
Cuts at: 0 5641 5886
Size: 5641 245
NcoI C'CATG_G
Cuts at: 0 915 934 948 1098 1248 3809 4849 5886
Size: 915 19 14 150 150 2561 1040 1037
Fragments arranged by size:
2561 1040 1037 915 150 150 19 14
NgoMIV G'CCGG_C
Cuts at: 0 5639 5886
Size: 5639 247
NheI G'CTAG_C
Cuts at: 0 1090 5886
Size: 1090 4796
NspI r_CATG'y
Cuts at: 0 971 2297 3063 3870 4041 5406 5514 5886
Size: 971 1326 766 807 171 1365 108 372
Fragments arranged by size:
1365 1326 971 807 766 372 171 108
PasI CC'CwG_GG
Cuts at: 0 2757 3188 5886
Size: 2757 431 2698
Fragments arranged by size:
2757 2698 431
PciI A'CATG_T
Cuts at: 0 967 3059 5510 5886
Size: 967 2092 2451 376
Fragments arranged by size:
2451 2092 967 376
PflMI CCAn_nnn'nTGG
Cuts at: 0 2957 3400 3419 4177 5886
Size: 2957 443 19 758 1709
Fragments arranged by size:
2957 1709 758 443 19
PfoI T'CCnGG_A
Cuts at: 0 5202 5886
Size: 5202 684
PmlI CAC'GTG
Cuts at: 0 2146 3308 4786 5886
Size: 2146 1162 1478 1100
Fragments arranged by size:
2146 1478 1162 1100
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1146 1178 1224 1256 5886
Size: 1146 32 46 32 4630
Fragments arranged by size:
4630 1146 46 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 637 2917 5157 5759 5788 5886
Size: 637 2280 2240 602 29 98
Fragments arranged by size:
2280 2240 637 602 98 29
PshAI GACnn'nnGTC
Cuts at: 0 642 3260 5886
Size: 642 2618 2626
Fragments arranged by size:
2626 2618 642
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2664 2696 5886
Size: 2664 32 3190
Fragments arranged by size:
3190 2664 32
PvuII CAG'CTG
Cuts at: 0 3123 3269 5309 5886
Size: 3123 146 2040 577
Fragments arranged by size:
3123 2040 577 146
RsrII CG'GwC_CG
Cuts at: 0 5681 5886
Size: 5681 205
SalI G'TCGA_C
Cuts at: 0 4331 5886
Size: 4331 1555
SanDI GG'GwC_CC
Cuts at: 0 5759 5788 5886
Size: 5759 29 98
Fragments arranged by size:
5759 98 29
ScaI AGT'ACT
Cuts at: 0 603 927 1188 4740 5886
Size: 603 324 261 3552 1146
Fragments arranged by size:
3552 1146 603 324 261
SfcI C'TryA_G
Cuts at: 0 263 1148 1190 2429 2882 4535 5466 5886
Size: 263 885 42 1239 453 1653 931 420
Fragments arranged by size:
1653 1239 931 885 453 420 263 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1613 5886
Size: 1613 4273
SmaI CCC'GGG
Cuts at: 0 4033 5886
Size: 4033 1853
SmlI C'TyrA_G
Cuts at: 0 2044 3470 4091 5886
Size: 2044 1426 621 1795
Fragments arranged by size:
2044 1795 1426 621
SpeI A'CTAG_T
Cuts at: 0 2677 5008 5886
Size: 2677 2331 878
Fragments arranged by size:
2677 2331 878
SphI G_CATG'C
Cuts at: 0 4041 5406 5886
Size: 4041 1365 480
Fragments arranged by size:
4041 1365 480
SspI AAT'ATT
Cuts at: 0 1176 1335 3741 5886
Size: 1176 159 2406 2145
Fragments arranged by size:
2406 2145 1176 159
StuI AGG'CCT
Cuts at: 0 382 2254 5886
Size: 382 1872 3632
Fragments arranged by size:
3632 1872 382
StyI C'CwwG_G
Cuts at: 0 483 915 934 948 1098 1248 2538 3809
Size: 483 432 19 14 150 150 1290 1271
Cuts at: 3809 3933 4849 5886
Size: 124 916 1037
Fragments arranged by size:
1290 1271 1037 916 483 432 150 150
124 19 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 5698 5854 5886
Size: 5698 156 32
Fragments arranged by size:
5698 156 32
TatI w'GTAC_w
Cuts at: 0 601 925 1186 1747 2023 3663 4738 5059
Size: 601 324 261 561 276 1640 1075 321
Cuts at: 5059 5517 5886
Size: 458 369
Fragments arranged by size:
1640 1075 601 561 458 369 324 321
276 261
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 813 845 1700 1732 1991 2023 5656 5688
Size: 813 32 855 32 259 32 3633 32
Cuts at: 5688 5886
Size: 198
Fragments arranged by size:
3633 855 813 259 198 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 4465 5106 5886
Size: 4465 641 780
Fragments arranged by size:
4465 780 641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3948 5306 5886
Size: 3948 1358 580
Fragments arranged by size:
3948 1358 580
XmaI C'CCGG_G
Cuts at: 0 4031 5886
Size: 4031 1855
XmnI GAAnn'nnTTC
Cuts at: 0 4453 5886
Size: 4453 1433
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AleI AlfI AloI
AlwNI ApaLI ApoI AseI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI Eco57MI EcoNI EcoO109I EcoRI FalI FspI Hin4I
HincII HindIII HpaI KpnI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PsrI PvuII RsrII
SalI SanDI ScaI SfcI SfiI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AgeI AhdI ApaI AscI
AsiSI AvrII BaeI BbeI BfrBI BlpI BmgBI BsiWI
BsmBI BsrBI BssHII BstBI BstEII BstZ17I EciI EcoICRI
EcoRV FseI FspAI HaeII KasI MfeI MluI NarI
NdeI NotI NruI NsiI PacI PmeI PsiI PspOMI
PspXI PstI PvuI SacI SacII SapI SbfI SexAI
SfoI SgfI SgrAI SnaBI SrfI SwaI XbaI XhoI
ZraI