Restriction map for
pFN21AB7424
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI Ksp632I KspI
MfeI MflI MluI MluNI MroI
MscI MspA1I MunI MvnI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI TaqI
Tth111I TthHB8I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 1375 GGATC GGATC(4/5)
BanII 272 GGGCTC G[GA]GC[CT]^C
BbsI 804 GTCTTC GAAGAC(2/6)
BcgI 499 GCAGCTTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 773 GGATAC GTATCC(6/5)
BclI 1026 TGATCA T^GATCA
BmyI 272 GGGCTC G[AGT]GC[ACT]^C
BpuAI 804 GTCTTC GAAGAC(2/6)
BseRI 431 CTCCTC GAGGAG(10/8)
Bsp1286I 272 GGGCTC G[AGT]GC[ACT]^C
BstXI 403 CCATTTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 979 GCATGC GC[ACGT]^[ACGT]GC
CfoI 1018 GCGC GCG^C
Cfr13I 178 GGGCC G^G[ACGT]CC
DrdI 6 GACTGTACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1008 GAATTC G^AATTC
FbaI 1026 TGATCA T^GATCA
HaeIII 179 GGCC GG^CC
HapII 151 CCGG C^CGG
HhaI 1018 GCGC GCG^C
HinP1I 1018 GCGC G^CGC
HpaII 151 CCGG C^CGG
MaeII 87 ACGT A^CGT
MboII 805 TCTTC GAAGA(8/7)
MslI 404 CATTTAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 151 CCGG C^CGG
NlaIV 273 GGCTCC GG[ACGT]^[ACGT]CC
PflMI 998 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 802 GAGTC GAGTC(4/5)
Sau96I 178 GGGCC G^G[ACGT]CC
ScaI 55 AGTACT AGT^ACT
SmlI 1197 CTCAAG C^T[CT][GA]AG
SphI 979 GCATGC GCATG^C
SspI 1401 AATATT AAT^ATT
SwaI 405 ATTTAAAT ATTT^AAAT
TfiI 28 GATTC G^A[AT]TC
Tsp45I 190 GTGAC ^GT[GC]AC
Van91I 998 CCAGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 94 CCACATGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 19 enzymes) #
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AcsI 990 AAATTC [GA]^AATT[CT]
1008 GAATTC
AlwNI 923 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1292 CAGTTCCTG
ApoI 990 AAATTC [GA]^AATT[CT]
1008 GAATTC
AseI 1048 ATTAAT AT^TAAT
1398 ATTAAT
AsnI 1048 ATTAAT AT^TAAT
1398 ATTAAT
BsaBI 28 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1339 GATTTTAATC
BsrI 1389 ACTGG ACTGG(1/-1)
778 CCAGT
BstAPI 886 GCAGTCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
922 GCAGCAACTGC
DdeI 342 CTTAG C^T[ACGT]AG
1013 CTCAG
DraI 406 TTTAAA TTT^AAA
1206 TTTAAA
DsaI 251 CCATGG C^C[GA][CT]GG
796 CCATGG
HinfI 28 GATTC G^A[ACGT]TC
802 GAGTC
HphI 597 GGTGA GGTGA(8/7)
578 TCACC
MamI 28 GATTCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1339 GATTTTAATC
NcoI 251 CCATGG C^CATGG
796 CCATGG
NspI 632 ACATGC [GA]CATG^[CT]
979 GCATGC
PshBI 1048 ATTAAT AT^TAAT
1398 ATTAAT
PstI 497 CTGCAG CTGCA^G
1434 CTGCAG
TspRI 78 CACTG CA[GC]TG[ACGT][ACGT]^
1120 CAGTG
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AfaI AluI BbvI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BssKI BstF5I BstNI DpnII EcoRII
EcoT14I Fnu4HI FokI ItaI MaeI
MaeIII MboI MnlI MseI MvaI
MwoI NdeII NlaIII RsaI Sau3AI
ScrFI SfaNI SfcI StyI Tru9I
TseI Tsp509I