(Circular) (Six-base) MAPSORT of: pFN21AB7424.seq Check: 9489 from: 1 to: 6151 pFN21AB7424 6151 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4214 5598 5866 1632 Size: 2582 1384 268 1917 Fragments arranged by size: 2582 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AlwNI CAG_nnn'CTG Cuts at: 2933 3302 3894 5466 2933 Size: 369 592 1572 3618 Fragments arranged by size: 3618 1572 592 369 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 2995 3013 3460 3686 3838 5947 1493 Size: 1502 18 447 226 152 2109 1697 Fragments arranged by size: 2109 1697 1502 447 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 3054 3404 4705 160 Size: 872 2022 350 1301 1606 Fragments arranged by size: 2022 1606 1301 872 350 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 3481 3848 3481 Size: 367 5784 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3472 4927 618 Size: 325 217 627 1685 1455 1842 Fragments arranged by size: 1842 1685 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2281 3470 1391 Size: 890 1189 4072 Fragments arranged by size: 4072 1189 890 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2802 928 Size: 971 903 4277 Fragments arranged by size: 4277 971 903 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2493 2527 3430 3464 4342 4376 1903 Size: 34 556 34 903 34 878 34 3678 Fragments arranged by size: 3678 903 878 556 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2772 4043 5253 2772 Size: 1271 1210 3670 Fragments arranged by size: 3670 1271 1210 BclI T'GATC_A Cuts at: 1548 3031 1548 Size: 1483 4668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4762 136 Size: 107 122 71 631 605 3090 1525 Fragments arranged by size: 3090 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 4212 5368 1790 Size: 2422 1156 2573 Fragments arranged by size: 2573 2422 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6151 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3882 4840 442 Size: 416 53 83 399 24 2465 958 1753 Fragments arranged by size: 2465 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2136 2138 2136 Size: 2 6149 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2293 2958 2986 4811 1239 Size: 1054 665 28 1825 2579 Fragments arranged by size: 2579 1825 1054 665 28 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3187 4276 5141 5439 3187 Size: 1089 865 298 3899 Fragments arranged by size: 3899 1089 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4814 882 Size: 617 3315 2219 Fragments arranged by size: 3315 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 2037 3348 6145 2037 Size: 1311 2797 2043 Fragments arranged by size: 2797 2043 1311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3958 4340 4975 275 Size: 53 83 186 936 2425 382 635 1451 Fragments arranged by size: 2425 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3078 3108 1178 Size: 30 165 30 1675 30 4221 Fragments arranged by size: 4221 1675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2427 1186 Size: 1241 4910 BseYI C'CCAG_C Cuts at: 3082 3189 5354 5850 3082 Size: 107 2165 496 3383 Fragments arranged by size: 3383 2165 496 107 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5578 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4212 4297 5368 4212 Size: 85 1071 4995 Fragments arranged by size: 4995 1071 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2281 3470 4212 4297 5368 1391 Size: 399 491 1189 742 85 1071 2174 Fragments arranged by size: 2174 1189 1071 742 491 399 85 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3506 844 Size: 2662 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4646 4820 65 Size: 1183 3398 174 1396 Fragments arranged by size: 3398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4795 4982 1700 Size: 110 2985 187 2869 Fragments arranged by size: 2985 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 2897 2933 794 Size: 2103 36 4012 Fragments arranged by size: 4012 2103 36 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6151 BstXI CCAn_nnnn'nTGG Cuts at: 2415 2415 Size: 6151 BstYI r'GATC_y Cuts at: 1972 3481 3848 4233 4250 5702 5788 5800 6146 Size: 1509 367 385 17 1452 86 12 346 Cuts at: 6146 1972 Size: 1977 Fragments arranged by size: 1977 1509 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2256 2801 513 Size: 552 1191 545 3863 Fragments arranged by size: 3863 1191 552 545 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 3667 4461 4481 3667 Size: 794 20 5337 Fragments arranged by size: 5337 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 2413 3213 3455 2413 Size: 800 242 5109 Fragments arranged by size: 5109 800 242 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2017 5158 809 Size: 507 701 3141 1802 Fragments arranged by size: 3141 1802 701 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3598 4487 6116 Size: 54 1425 50 275 18 1768 889 1629 Cuts at: 6116 8 Size: 43 Fragments arranged by size: 1768 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4081 1353 Size: 2728 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3053 4738 5112 5258 387 Size: 1064 138 1464 1685 374 146 1280 Fragments arranged by size: 1685 1464 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2293 2958 2986 4214 4811 5598 5866 Size: 393 661 665 28 1228 597 787 268 Cuts at: 5866 1239 Size: 1524 Fragments arranged by size: 1524 1228 787 665 661 597 393 268 28 EcoRI G'AATT_C Cuts at: 3013 3460 3838 3013 Size: 447 378 5326 Fragments arranged by size: 5326 447 378 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 5298 1678 Size: 3620 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2931 2963 3043 3075 4792 Size: 32 135 32 1359 32 80 32 1717 Cuts at: 4792 4824 4866 4898 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1717 1359 135 80 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6151 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2629 5265 5449 1305 Size: 1324 2636 184 2007 Fragments arranged by size: 2636 2007 1324 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4329 Fragments arranged by size: 4329 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2413 4109 4468 4627 518 Size: 648 1247 1696 359 159 2042 Fragments arranged by size: 2042 1696 1247 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3567 4244 5392 5637 1606 Size: 1961 677 1148 245 2120 Fragments arranged by size: 2120 1961 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2256 2801 513 Size: 552 1191 545 3863 Fragments arranged by size: 3863 1191 552 545 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2641 2988 3509 5054 1132 Size: 97 1412 347 521 1545 2229 Fragments arranged by size: 2229 1545 1412 521 347 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 3009 1108 Size: 1901 4250 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4220 4252 5733 5765 4220 Size: 32 1481 32 4606 Fragments arranged by size: 4606 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3171 3721 3171 Size: 550 5601 PstI C_TGCA'G Cuts at: 830 2506 3443 3503 830 Size: 1676 937 60 3478 Fragments arranged by size: 3478 1676 937 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SbfI CC_TGCA'GG Cuts at: 3503 3503 Size: 6151 ScaI AGT'ACT Cuts at: 2062 4399 6111 2062 Size: 2337 1712 2102 Fragments arranged by size: 2337 2102 1712 SfcI C'TryA_G Cuts at: 826 1045 2502 2682 3439 3499 4634 5315 5506 Size: 219 1457 180 757 60 1135 681 191 Cuts at: 5506 826 Size: 1471 Fragments arranged by size: 1471 1457 1135 757 681 219 191 180 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3202 4255 5156 5418 820 Size: 197 926 1259 1053 901 262 1553 Fragments arranged by size: 1553 1259 1053 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 2988 3509 1132 Size: 1856 521 3774 Fragments arranged by size: 3774 1856 521 SspI AAT'ATT Cuts at: 5 52 3408 4075 5 Size: 47 3356 667 2081 Fragments arranged by size: 3356 2081 667 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2229 2256 2801 3237 3590 513 Size: 552 1164 27 545 436 353 3074 Fragments arranged by size: 3074 1164 552 545 436 353 27 SwaI ATTT'AAAT Cuts at: 2413 2413 Size: 6151 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2355 2584 2590 4178 4195 4348 4533 Size: 1255 775 229 6 1588 17 153 185 Cuts at: 4533 4978 325 Size: 445 1498 Fragments arranged by size: 1588 1498 1255 775 445 229 185 153 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2060 2239 4397 Size: 274 80 33 51 1443 83 179 2158 Cuts at: 4397 6109 96 Size: 1712 138 Fragments arranged by size: 2158 1712 1443 274 179 138 83 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6111 6143 6111 Size: 32 6119 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6151 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2106 2106 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 4280 4280 Size: 6151 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI