(Circular) (Six-base) MAPSORT of: pFN21AB9977.seq Check: 4286 from: 1 to: 6376 pFN21AB9977 6376 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2069 2069 Size: 6376 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2118 3719 1937 Size: 181 1601 4594 Fragments arranged by size: 4594 1601 181 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 2892 4503 4876 1563 Size: 1329 1611 373 3063 Fragments arranged by size: 3063 1611 1329 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2436 2508 2982 4439 5823 6091 1632 Size: 804 72 474 1457 1384 268 1917 Fragments arranged by size: 1917 1457 1384 804 474 268 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 5275 6252 1203 Size: 4072 977 1327 Fragments arranged by size: 4072 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 3083 1166 Size: 1917 4459 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 4119 5691 4119 Size: 1572 4804 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 2568 2907 3312 3330 3685 3911 4063 6172 Size: 1075 339 405 18 355 226 152 2109 Cuts at: 6172 1493 Size: 1697 Fragments arranged by size: 2109 1697 1075 405 355 339 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4930 160 Size: 872 3898 1606 Fragments arranged by size: 3898 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3701 1870 Size: 73 1758 4545 Fragments arranged by size: 4545 1758 73 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2053 3697 5152 618 Size: 325 217 627 266 1644 1455 1842 Fragments arranged by size: 1842 1644 1455 627 325 266 217 BanII G_rGCy'C Cuts at: 1391 3695 1391 Size: 2304 4072 BbeI G_GCGC'C Cuts at: 2057 2057 Size: 6376 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3373 3397 928 Size: 971 1474 24 3907 Fragments arranged by size: 3907 1474 971 24 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2020 2054 4567 4601 1903 Size: 34 83 34 2513 34 3678 Fragments arranged by size: 3678 2513 83 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 1548 Size: 6376 BfrBI ATG'CAT Cuts at: 3393 3393 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4987 136 Size: 107 122 71 631 605 3315 1525 Fragments arranged by size: 3315 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 3793 3793 Size: 6376 Bme1580I G_kGCm'C Cuts at: 1790 4437 5593 1790 Size: 2647 1156 2573 Fragments arranged by size: 2647 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2217 2866 4107 Size: 416 53 83 399 24 800 649 1241 Cuts at: 4107 5065 442 Size: 958 1753 Fragments arranged by size: 1753 1241 958 800 649 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6376 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2374 3466 2374 Size: 1092 5284 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2379 2991 5036 1239 Size: 1140 612 2045 2579 Fragments arranged by size: 2579 2045 1140 612 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2264 3406 4501 5366 5664 2264 Size: 1142 1095 865 298 2976 Fragments arranged by size: 2976 1142 1095 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5039 882 Size: 617 3540 2219 Fragments arranged by size: 3540 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 2438 3570 6370 2438 Size: 1132 2800 2444 Fragments arranged by size: 2800 2444 1132 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2054 4183 4565 5200 Size: 53 83 186 936 521 2129 382 635 Cuts at: 5200 275 Size: 1451 Fragments arranged by size: 2129 1451 936 635 521 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6151 Fragments arranged by size: 6151 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2091 1186 Size: 905 5471 BseYI C'CCAG_C Cuts at: 5579 6075 5579 Size: 496 5880 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5803 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2043 3061 3229 4437 4522 5593 2043 Size: 1018 168 1208 85 1071 2826 Fragments arranged by size: 2826 1208 1071 1018 168 85 BsmI GAATG_Cn' Cuts at: 3887 3980 3887 Size: 93 6283 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 3061 3229 3695 4437 4522 5593 Size: 399 253 1018 168 466 742 85 1071 Cuts at: 5593 1391 Size: 2174 Fragments arranged by size: 2174 1071 1018 742 466 399 253 168 85 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2069 3731 844 Size: 1225 1662 3489 Fragments arranged by size: 3489 1662 1225 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4871 5045 65 Size: 1183 3623 174 1396 Fragments arranged by size: 3623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5020 5207 1700 Size: 110 3210 187 2869 Fragments arranged by size: 3210 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 3347 794 Size: 2553 3823 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6376 BstYI r'GATC_y Cuts at: 1972 3023 3706 4073 4458 4475 5927 6013 6025 Size: 1051 683 367 385 17 1452 86 12 Cuts at: 6025 6371 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1051 683 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2144 2144 Size: 6376 BtgI C'CryG_G Cuts at: 513 1065 2049 2057 3234 513 Size: 552 984 8 1177 3655 Fragments arranged by size: 3655 1177 984 552 8 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2175 487 Size: 683 56 949 4688 Fragments arranged by size: 4688 949 683 56 BtsI GCAGTG_nn' Cuts at: 3265 3892 4686 4706 3265 Size: 627 794 20 4935 Fragments arranged by size: 4935 794 627 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2765 2800 529 Size: 35 2201 35 4105 Fragments arranged by size: 4105 2201 35 35 DraI TTT'AAA Cuts at: 2802 3680 2802 Size: 878 5498 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3823 4712 6341 Size: 54 1425 50 275 18 1993 889 1629 Cuts at: 6341 8 Size: 43 Fragments arranged by size: 1993 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2527 2958 4306 1353 Size: 1174 431 1348 3423 Fragments arranged by size: 3423 1348 1174 431 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4963 5337 5483 387 Size: 1064 138 3374 374 146 1280 Fragments arranged by size: 3374 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2379 2436 2508 2982 2991 4439 5036 Size: 393 747 57 72 474 9 1448 597 Cuts at: 5036 5823 6091 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1448 787 747 597 474 393 268 72 57 9 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2057 5523 1678 Size: 379 3466 2531 Fragments arranged by size: 3466 2531 379 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2377 2409 5017 5049 5091 Size: 32 135 32 805 32 2608 32 42 Cuts at: 5091 5123 1373 Size: 32 2626 Fragments arranged by size: 2626 2608 805 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2119 2356 3720 3966 669 Size: 988 281 181 237 1364 246 3079 Fragments arranged by size: 3079 1364 988 281 246 237 181 HindIII A'AGCT_T Cuts at: 748 2488 2991 748 Size: 1740 503 4133 Fragments arranged by size: 4133 1740 503 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KasI G'GCGC_C Cuts at: 2053 2053 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3534 3975 3534 Size: 441 5935 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3307 5490 5674 1305 Size: 2002 2183 184 2007 Fragments arranged by size: 2183 2007 2002 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4554 Fragments arranged by size: 4554 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3083 4334 4693 4852 518 Size: 648 1917 1251 359 159 2042 Fragments arranged by size: 2042 1917 1251 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3792 4469 5617 5862 1606 Size: 2186 677 1148 245 2120 Fragments arranged by size: 2186 2120 1148 677 245 NarI GG'CG_CC Cuts at: 2054 2054 Size: 6376 NcoI C'CATG_G Cuts at: 513 1065 2057 3234 513 Size: 552 992 1177 3655 Fragments arranged by size: 3655 1177 992 552 NdeI CA'TA_TG Cuts at: 387 2758 387 Size: 2371 4005 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6376 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NsiI A_TGCA'T Cuts at: 3395 3395 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3734 5279 1132 Size: 97 2505 1545 2229 Fragments arranged by size: 2505 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6376 PfoI T'CCnGG_A Cuts at: 2446 2812 3090 2446 Size: 366 278 5732 Fragments arranged by size: 5732 366 278 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 2574 3946 2574 Size: 1372 5004 PstI C_TGCA'G Cuts at: 830 3728 830 Size: 2898 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 SalI G'TCGA_C Cuts at: 1936 2117 3718 1936 Size: 181 1601 4594 Fragments arranged by size: 4594 1601 181 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2647 3540 3659 3724 4859 5540 5731 Size: 219 1602 893 119 65 1135 681 191 Cuts at: 5731 826 Size: 1471 Fragments arranged by size: 1602 1471 1135 893 681 219 191 119 65 SfoI GGC'GCC Cuts at: 2055 2055 Size: 6376 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2279 3385 4480 5381 5643 820 Size: 197 926 336 1106 1095 901 262 1553 Fragments arranged by size: 1553 1106 1095 926 901 336 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 2252 152 Size: 2100 4276 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 2248 2551 4300 5 Size: 47 2196 303 1749 2081 Fragments arranged by size: 2196 2081 1749 303 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2057 3234 3815 513 Size: 552 992 1177 581 3074 Fragments arranged by size: 3074 1177 992 581 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2050 2226 4403 4420 4573 4758 5203 Size: 1255 470 176 2177 17 153 185 445 Cuts at: 5203 325 Size: 1498 Fragments arranged by size: 2177 1498 1255 470 445 185 176 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2401 2656 4622 Size: 274 80 33 51 1443 424 255 1966 Cuts at: 4622 6334 96 Size: 1712 138 Fragments arranged by size: 1966 1712 1443 424 274 255 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2626 2658 6336 6368 2626 Size: 32 3678 32 2634 Fragments arranged by size: 3678 2634 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 3380 3616 4505 3380 Size: 236 889 5251 Fragments arranged by size: 5251 889 236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI