(Circular) (Six-base) MAPSORT of: pFN21AB9975.seq Check: 3186 from: 1 to: 6298 pFN21AB9975 6298 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3576 3576 Size: 6298 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2214 4108 278 Size: 53 83 186 936 678 1894 2468 Fragments arranged by size: 2468 1894 936 678 186 83 53 AccI GT'mk_AC Cuts at: 1937 3084 3641 1937 Size: 1147 557 4594 Fragments arranged by size: 4594 1147 557 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2297 2432 4361 5745 6013 1632 Size: 665 135 1929 1384 268 1917 Fragments arranged by size: 1929 1917 1384 665 268 135 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 2333 2351 1166 Size: 1167 18 5113 Fragments arranged by size: 5113 1167 18 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6298 AlwNI CAG_nnn'CTG Cuts at: 2677 3280 4041 5613 2677 Size: 603 761 1572 3362 Fragments arranged by size: 3362 1572 761 603 ApaI G_GGCC'C Cuts at: 2446 2446 Size: 6298 ApaLI G'TGCA_C Cuts at: 4355 5511 4355 Size: 1156 5142 ApoI r'AATT_y Cuts at: 1493 3010 3607 3833 3985 6094 1493 Size: 1517 597 226 152 2109 1697 Fragments arranged by size: 2109 1697 1517 597 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 2989 3551 3623 1870 Size: 73 1046 562 72 4545 Fragments arranged by size: 4545 1046 562 73 72 BamHI G'GATC_C Cuts at: 3628 3995 3628 Size: 367 5931 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2921 3619 5074 618 Size: 325 217 627 1134 698 1455 1842 Fragments arranged by size: 1842 1455 1134 698 627 325 217 BanII G_rGCy'C Cuts at: 1391 2446 2549 3332 3503 3617 1391 Size: 1055 103 783 171 114 4072 Fragments arranged by size: 4072 1055 783 171 114 103 BbeI G_GCGC'C Cuts at: 2925 2925 Size: 6298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2420 928 Size: 971 521 4806 Fragments arranged by size: 4806 971 521 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 1548 Size: 6298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2450 2459 2507 Size: 107 122 71 631 605 778 9 48 Cuts at: 2507 2936 3170 4909 136 Size: 429 234 1739 1525 Fragments arranged by size: 1739 1525 778 631 605 429 234 122 107 71 48 9 BglII A'GATC_T Cuts at: 6293 6293 Size: 6298 BlpI GC'TnA_GC Cuts at: 3715 3715 Size: 6298 Bme1580I G_kGCm'C Cuts at: 1790 2446 3195 4359 5515 1790 Size: 656 749 1164 1156 2573 Fragments arranged by size: 2573 1164 1156 749 656 BmgBI CAC'GTC Cuts at: 1318 3094 1318 Size: 1776 4522 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4029 4987 442 Size: 416 53 83 399 24 2612 958 1753 Fragments arranged by size: 2612 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2080 2080 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4958 1239 Size: 3719 2579 Bpu10I CC'TnA_GC Cuts at: 2555 2555 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3398 4423 5288 5586 3398 Size: 1025 865 298 4110 Fragments arranged by size: 4110 1025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3580 4961 882 Size: 617 2081 1381 2219 Fragments arranged by size: 2219 2081 1381 617 BsaAI yAC'GTr Cuts at: 493 1204 4089 493 Size: 711 2885 2702 Fragments arranged by size: 2885 2702 711 BsaBI GATnn'nnATC Cuts at: 2189 2984 6292 2189 Size: 795 3308 2195 Fragments arranged by size: 3308 2195 795 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 2211 2922 3081 Size: 53 83 186 936 636 42 711 159 Cuts at: 3081 3441 4105 4487 5122 275 Size: 360 664 382 635 1451 Fragments arranged by size: 1451 936 711 664 636 635 382 360 186 159 83 53 42 BsaWI w'CCGG_w Cuts at: 2193 4731 5129 5403 5550 5961 2193 Size: 2538 398 274 147 411 2530 Fragments arranged by size: 2538 2530 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6073 Fragments arranged by size: 6073 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2330 3419 3524 1186 Size: 1144 1089 105 3960 Fragments arranged by size: 3960 1144 1089 105 BseYI C'CCAG_C Cuts at: 2706 3165 5501 5997 2706 Size: 459 2336 496 3007 Fragments arranged by size: 3007 2336 496 459 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2184 2588 2740 3116 3161 3185 3266 Size: 573 448 404 152 376 45 24 81 Cuts at: 3266 3293 3356 3521 1163 Size: 27 63 165 3940 Fragments arranged by size: 3940 573 448 404 376 165 152 81 63 45 27 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2514 3748 4509 5537 1540 Size: 275 186 513 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 513 275 186 BsiHKAI G_wGCw'C Cuts at: 2556 4359 4444 5515 2556 Size: 1803 85 1071 3339 Fragments arranged by size: 3339 1803 1071 85 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2446 2549 2556 3195 3332 3503 3617 Size: 399 656 103 7 639 137 171 114 Cuts at: 3617 4359 4444 5515 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 656 639 399 171 137 114 103 85 7 BspEI T'CCGG_A Cuts at: 2193 5961 2193 Size: 3768 2530 BspHI T'CATG_A Cuts at: 2094 4187 5917 2094 Size: 2093 1730 2475 Fragments arranged by size: 2475 2093 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2850 2963 3543 3576 3653 844 Size: 2006 113 580 33 77 3489 Fragments arranged by size: 3489 2006 580 113 77 33 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3439 4793 4967 65 Size: 1183 2191 1354 174 1396 Fragments arranged by size: 2191 1396 1354 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4942 5129 1700 Size: 110 3132 187 2869 Fragments arranged by size: 3132 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6298 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 4358 5370 4358 Size: 1012 5286 BstAPI GCAn_nnn'nTGC Cuts at: 794 3199 794 Size: 2405 3893 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 2257 2353 1184 Size: 1073 96 5129 Fragments arranged by size: 5129 1073 96 BstXI CCAn_nnnn'nTGG Cuts at: 2332 2332 Size: 6298 BstYI r'GATC_y Cuts at: 1972 2048 3108 3409 3628 3995 4380 4397 5849 Size: 76 1060 301 219 367 385 17 1452 Cuts at: 5849 5935 5947 6293 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1060 385 367 346 301 219 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2331 2514 2880 2886 2917 3504 Size: 552 939 327 183 366 6 31 587 Cuts at: 3504 513 Size: 3307 Fragments arranged by size: 3307 939 587 552 366 327 183 31 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2546 487 Size: 683 56 1320 4239 Fragments arranged by size: 4239 1320 683 56 BtsI GCAGTG_nn' Cuts at: 3814 4608 4628 3814 Size: 794 20 5484 Fragments arranged by size: 5484 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2015 5305 809 Size: 507 699 3290 1802 Fragments arranged by size: 3290 1802 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2371 2511 2535 Size: 54 1425 50 275 18 541 140 24 Cuts at: 2535 2883 2937 3171 3363 3745 4634 6263 8 Size: 348 54 234 192 382 889 1629 43 Fragments arranged by size: 1629 1425 889 541 382 348 275 234 192 140 54 54 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 2511 3745 1537 Size: 275 699 1234 4090 Fragments arranged by size: 4090 1234 699 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2582 4228 1353 Size: 1229 1646 3423 Fragments arranged by size: 3423 1646 1229 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3164 4885 5259 5405 387 Size: 1064 138 1575 1721 374 146 1280 Fragments arranged by size: 1721 1575 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2297 2432 4361 4958 5745 6013 1239 Size: 393 665 135 1929 597 787 268 1524 Fragments arranged by size: 1929 1524 787 665 597 393 268 135 EcoNI CCTnn'n_nnAGG Cuts at: 3284 3284 Size: 6298 EcoO109I rG'GnC_Cy Cuts at: 2674 3277 3418 3523 2674 Size: 603 141 105 5449 Fragments arranged by size: 5449 603 141 105 EcoRI G'AATT_C Cuts at: 3607 3985 3607 Size: 378 5920 FspI TGC'GCA Cuts at: 2765 4804 2765 Size: 2039 4259 HaeII r_GCGC'y Cuts at: 1678 2706 2925 5445 1678 Size: 1028 219 2520 2531 Fragments arranged by size: 2531 2520 1028 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2076 2108 4939 4971 5013 Size: 32 135 32 504 32 2831 32 42 Cuts at: 5013 5045 1373 Size: 32 2626 Fragments arranged by size: 2831 2626 504 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3888 669 Size: 988 281 1704 246 3079 Fragments arranged by size: 3079 1704 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 2921 2921 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2421 2957 3405 5412 5596 1305 Size: 1116 536 448 2007 184 2007 Fragments arranged by size: 2007 2007 1116 536 448 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2939 3173 10 Size: 54 1425 343 1107 234 3135 Fragments arranged by size: 3135 1425 1107 343 234 54 MslI CAynn'nnrTG Cuts at: 518 1166 2303 2333 2351 4256 4615 4774 518 Size: 648 1137 30 18 1905 359 159 2042 Fragments arranged by size: 2042 1905 1137 648 359 159 30 18 MspA1I CmG'CkG Cuts at: 1606 2888 2963 3169 3250 3714 4391 5539 5784 Size: 1282 75 206 81 464 677 1148 245 Cuts at: 5784 1606 Size: 2120 Fragments arranged by size: 2120 1282 1148 677 464 245 206 81 75 NarI GG'CG_CC Cuts at: 2922 2922 Size: 6298 NcoI C'CATG_G Cuts at: 513 1065 2004 2331 2880 513 Size: 552 939 327 549 3931 Fragments arranged by size: 3931 939 552 549 327 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 3656 5201 1132 Size: 97 2427 1545 2229 Fragments arranged by size: 2427 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2236 1348 Size: 888 5410 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 2881 1108 Size: 1773 4525 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3418 3418 Size: 6298 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PspOMI G'GGCC_C Cuts at: 2442 2442 Size: 6298 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2564 2596 2564 Size: 32 6266 PstI C_TGCA'G Cuts at: 830 2119 2460 2685 3650 830 Size: 1289 341 225 965 3478 Fragments arranged by size: 3478 1289 965 341 225 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 2963 3169 3250 2963 Size: 206 81 6011 Fragments arranged by size: 6011 206 81 RsrII CG'GwC_CG Cuts at: 2084 2084 Size: 6298 SacII CC_GC'GG Cuts at: 2889 2889 Size: 6298 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SbfI CC_TGCA'GG Cuts at: 2460 3650 2460 Size: 1190 5108 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SfcI C'TryA_G Cuts at: 826 1045 2115 2456 2681 3646 4781 5462 5653 Size: 219 1070 341 225 965 1135 681 191 Cuts at: 5653 826 Size: 1471 Fragments arranged by size: 1471 1135 1070 965 681 341 225 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2450 2936 3170 2450 Size: 486 234 5578 Fragments arranged by size: 5578 486 234 SfoI GGC'GCC Cuts at: 2923 2923 Size: 6298 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 2991 3625 1872 Size: 1119 634 4545 Fragments arranged by size: 4545 1119 634 SmlI C'TyrA_G Cuts at: 820 1017 1943 3413 4402 5303 5565 820 Size: 197 926 1470 989 901 262 1553 Fragments arranged by size: 1553 1470 989 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SspI AAT'ATT Cuts at: 5 52 4222 5 Size: 47 4170 2081 Fragments arranged by size: 4170 2081 47 StuI AGG'CCT Cuts at: 1517 2294 1517 Size: 777 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2331 2493 2880 3285 3737 513 Size: 552 939 327 162 387 405 452 3074 Fragments arranged by size: 3074 939 552 452 405 387 327 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2432 2461 4325 4342 4495 4680 5125 Size: 1255 852 29 1864 17 153 185 445 Cuts at: 5125 325 Size: 1498 Fragments arranged by size: 1864 1498 1255 852 445 185 153 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2802 4544 6256 Size: 274 80 33 51 1443 825 1742 1712 Cuts at: 6256 96 Size: 138 Fragments arranged by size: 1742 1712 1443 825 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6258 6290 6258 Size: 32 6266 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6298 XbaI T'CTAG_A Cuts at: 2177 3634 2177 Size: 1457 4841 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2722 2992 2722 Size: 270 6028 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 2989 3623 1870 Size: 1119 634 4545 Fragments arranged by size: 4545 1119 634 XmnI GAAnn'nnTTC Cuts at: 2576 3350 4427 2576 Size: 774 1077 4447 Fragments arranged by size: 4447 1077 774 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2212 4106 276 Size: 53 83 186 936 678 1894 2468 Fragments arranged by size: 2468 1894 936 678 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI SacI SanDI SapI SexAI SrfI SwaI