(Circular) (Six-base) MAPSORT of: pFN21AB9972.seq Check: 8135 from: 1 to: 6817 pFN21AB9972 6817 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4627 278 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4160 1937 Size: 2223 4594 Acc65I G'GTAC_C Cuts at: 4138 4138 Size: 6817 AclI AA'CG_TT Cuts at: 1563 4944 5317 1563 Size: 3381 373 3063 Fragments arranged by size: 3381 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4880 6264 6532 1632 Size: 3248 1384 268 1917 Fragments arranged by size: 3248 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6620 AflIII A'CryG_T Cuts at: 1203 4114 5716 6693 1203 Size: 2911 1602 977 1327 Fragments arranged by size: 2911 1602 1327 977 AgeI A'CCGG_T Cuts at: 5648 5648 Size: 6817 AhdI GACnn_n'nnGTC Cuts at: 5546 5546 Size: 6817 AleI CACnn'nnGTG Cuts at: 1166 3621 3791 1166 Size: 2455 170 4192 Fragments arranged by size: 4192 2455 170 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2463 1184 Size: 1279 5538 AlwNI CAG_nnn'CTG Cuts at: 3268 3883 3916 4006 4560 6132 3268 Size: 615 33 90 554 1572 3953 Fragments arranged by size: 3953 1572 615 554 90 33 ApaI G_GGCC'C Cuts at: 2138 2138 Size: 6817 ApaLI G'TGCA_C Cuts at: 4874 6030 4874 Size: 1156 5661 ApoI r'AATT_y Cuts at: 1493 3275 4126 4352 4504 6613 1493 Size: 1782 851 226 152 2109 1697 Fragments arranged by size: 2109 1782 1697 851 226 152 AseI AT'TA_AT Cuts at: 160 1032 5371 160 Size: 872 4339 1606 Fragments arranged by size: 4339 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 AvaI C'yCGr_G Cuts at: 1870 1943 2674 2808 4142 1870 Size: 73 731 134 1334 4545 Fragments arranged by size: 4545 1334 731 134 73 AvrII C'CTAG_G Cuts at: 4061 4061 Size: 6817 BamHI G'GATC_C Cuts at: 4147 4514 4147 Size: 367 6450 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2065 2459 3482 4138 5593 Size: 325 217 627 278 394 1023 656 1455 Cuts at: 5593 618 Size: 1842 Fragments arranged by size: 1842 1455 1023 656 627 394 325 278 217 BanII G_rGCy'C Cuts at: 1391 2138 3086 3606 3750 4136 1391 Size: 747 948 520 144 386 4072 Fragments arranged by size: 4072 948 747 520 386 144 BbeI G_GCGC'C Cuts at: 2069 2069 Size: 6817 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5846 BbvCI CC'TCA_GC Cuts at: 2577 3203 2577 Size: 626 6191 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2578 2612 5008 5042 1903 Size: 34 641 34 2396 34 3678 Fragments arranged by size: 3678 2396 641 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2470 4709 5919 2470 Size: 2239 1210 3368 Fragments arranged by size: 3368 2239 1210 BclI T'GATC_A Cuts at: 1548 3270 1548 Size: 1722 5095 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3151 3436 5428 Size: 107 122 71 631 605 1479 285 1992 Cuts at: 5428 136 Size: 1525 Fragments arranged by size: 1992 1525 1479 631 605 285 122 107 71 BglII A'GATC_T Cuts at: 6812 6812 Size: 6817 BlpI GC'TnA_GC Cuts at: 3999 4234 3999 Size: 235 6582 Bme1580I G_kGCm'C Cuts at: 1790 2138 3042 4878 6034 1790 Size: 348 904 1836 1156 2573 Fragments arranged by size: 2573 1836 1156 904 348 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6817 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2122 4548 5506 Size: 416 53 83 399 24 705 2426 958 Cuts at: 5506 442 Size: 1753 Fragments arranged by size: 2426 1753 958 705 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6817 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2371 3100 2371 Size: 729 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2733 3070 3863 3950 4127 5477 1239 Size: 937 557 337 793 87 177 1350 2579 Fragments arranged by size: 2579 1350 937 793 557 337 177 87 Bpu10I CC'TnA_GC Cuts at: 2577 3203 2577 Size: 626 6191 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4942 5807 6105 4942 Size: 865 298 5654 Fragments arranged by size: 5654 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5480 882 Size: 617 3981 2219 Fragments arranged by size: 3981 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3037 4608 493 Size: 711 1833 1571 2702 Fragments arranged by size: 2702 1833 1571 711 BsaBI GATnn'nnATC Cuts at: 6811 6811 Size: 6817 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2066 4624 5006 5641 Size: 53 83 186 936 533 2558 382 635 Cuts at: 5641 275 Size: 1451 Fragments arranged by size: 2558 1451 936 635 533 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5250 5648 5922 6069 6480 5250 Size: 398 274 147 411 5587 Fragments arranged by size: 5587 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2671 2701 3677 3707 1178 Size: 30 165 30 1268 30 976 30 4288 Fragments arranged by size: 4288 1268 976 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2954 3701 3758 3914 1186 Size: 1768 747 57 156 4089 Fragments arranged by size: 4089 1768 747 156 57 BseYI C'CCAG_C Cuts at: 2149 3079 3709 3772 6020 6516 2149 Size: 930 630 63 2248 496 2450 Fragments arranged by size: 2450 2248 930 630 496 63 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2201 2267 2975 3599 1163 Size: 573 465 66 708 624 4381 Fragments arranged by size: 4381 708 624 573 465 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2082 4267 5028 6056 1540 Size: 275 186 81 2185 761 1028 2301 Fragments arranged by size: 2301 2185 1028 761 275 186 81 BsiHKAI G_wGCw'C Cuts at: 3534 3606 3750 4878 4963 6034 3534 Size: 72 144 1128 85 1071 4317 Fragments arranged by size: 4317 1128 1071 144 85 72 BsmI GAATG_Cn' Cuts at: 4328 4421 4328 Size: 93 6724 BsmBI CGTCTCn'nnnn_ Cuts at: 3650 3731 3650 Size: 81 6736 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 3042 3086 3534 3606 3750 4136 Size: 399 348 904 44 448 72 144 386 Cuts at: 4136 4878 4963 6034 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 904 742 448 399 386 348 144 85 72 44 BspEI T'CCGG_A Cuts at: 6480 6480 Size: 6817 BspHI T'CATG_A Cuts at: 4706 6436 4706 Size: 1730 5087 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4172 844 Size: 3328 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2664 3013 4704 1394 Size: 429 841 349 1691 3507 Fragments arranged by size: 3507 1691 841 429 349 BsrDI GCAATG_nn' Cuts at: 65 1248 5312 5486 65 Size: 1183 4064 174 1396 Fragments arranged by size: 4064 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2068 3479 5461 5648 1700 Size: 110 258 1411 1982 187 2869 Fragments arranged by size: 2869 1982 1411 258 187 110 BsrGI T'GTAC_A Cuts at: 96 3099 96 Size: 3003 3814 BssHII G'CGCG_C Cuts at: 1925 2009 3904 3976 1925 Size: 84 1895 72 4766 Fragments arranged by size: 4766 1895 84 72 BssSI C'ACGA_G Cuts at: 4877 5889 4877 Size: 1012 5805 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6817 BstBI TT'CG_AA Cuts at: 5621 5621 Size: 6817 BstEII G'GTnAC_C Cuts at: 1184 2687 3799 3832 1184 Size: 1503 1112 33 4169 Fragments arranged by size: 4169 1503 1112 33 BstXI CCAn_nnnn'nTGG Cuts at: 2480 2480 Size: 6817 BstYI r'GATC_y Cuts at: 1972 4147 4514 4899 4916 6368 6454 6466 6812 Size: 2175 367 385 17 1452 86 12 346 Cuts at: 6812 1972 Size: 1977 Fragments arranged by size: 2175 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3132 3828 4068 513 Size: 552 939 1128 696 240 3262 Fragments arranged by size: 3262 1128 939 696 552 240 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2382 487 Size: 683 56 1156 4922 Fragments arranged by size: 4922 1156 683 56 BtsI GCAGTG_nn' Cuts at: 2414 4333 5127 5147 2414 Size: 1919 794 20 4084 Fragments arranged by size: 4084 1919 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6817 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6782 DraI TTT'AAA Cuts at: 4121 4121 Size: 6817 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5824 809 Size: 507 4508 1802 Fragments arranged by size: 4508 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2407 2599 Size: 54 1425 50 275 18 249 328 192 Cuts at: 2599 3885 4264 5153 6782 8 Size: 1286 379 889 1629 43 Fragments arranged by size: 1629 1425 1286 889 379 328 275 249 192 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2079 4264 1537 Size: 275 267 2185 4090 Fragments arranged by size: 4090 2185 275 267 EarI CTCTTCn'nnn_ Cuts at: 1353 2496 3584 4747 1353 Size: 1143 1088 1163 3423 Fragments arranged by size: 3423 1163 1143 1088 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2890 5404 5778 5924 387 Size: 1064 138 1301 2514 374 146 1280 Fragments arranged by size: 2514 1301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3604 3748 3604 Size: 144 6673 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2733 3070 3863 3950 4127 4880 Size: 393 544 557 337 793 87 177 753 Cuts at: 4880 5477 6264 6532 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 793 787 753 597 557 544 393 337 268 177 87 EcoO109I rG'GnC_Cy Cuts at: 2135 3705 4102 2135 Size: 1570 397 4850 Fragments arranged by size: 4850 1570 397 EcoRI G'AATT_C Cuts at: 4126 4504 4126 Size: 378 6439 FspI TGC'GCA Cuts at: 3872 5323 3872 Size: 1451 5366 HaeII r_GCGC'y Cuts at: 1678 2027 2069 2075 3428 5964 1678 Size: 349 42 6 1353 2536 2531 Fragments arranged by size: 2536 2531 1353 349 42 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 2637 2669 3689 Size: 32 135 32 923 32 110 32 1020 Cuts at: 3689 3710 3721 3742 5458 5490 5532 5564 1373 Size: 21 11 21 1716 32 42 32 2626 Fragments arranged by size: 2626 1716 1020 923 135 110 42 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 4161 4407 669 Size: 988 281 2223 246 3079 Fragments arranged by size: 3079 2223 988 281 246 HindIII A'AGCT_T Cuts at: 748 2291 2733 748 Size: 1543 442 4832 Fragments arranged by size: 4832 1543 442 HpaI GTT'AAC Cuts at: 4407 4407 Size: 6817 KasI G'GCGC_C Cuts at: 2065 2065 Size: 6817 KpnI G_GTAC'C Cuts at: 4142 4142 Size: 6817 MfeI C'AATT_G Cuts at: 4416 4416 Size: 6817 MluI A'CGCG_T Cuts at: 6693 6693 Size: 6817 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2538 2805 3832 5931 6115 1305 Size: 1233 267 1027 2099 184 2007 Fragments arranged by size: 2099 2007 1233 1027 267 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3887 10 Size: 54 1425 343 2055 2940 Fragments arranged by size: 2940 2055 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2900 3621 3668 3791 4775 5134 5293 Size: 648 1734 721 47 123 984 359 159 Cuts at: 5293 518 Size: 2042 Fragments arranged by size: 2042 1734 984 721 648 359 159 123 47 MspA1I CmG'CkG Cuts at: 1606 3883 3973 4003 4233 4910 6058 6303 1606 Size: 2277 90 30 230 677 1148 245 2120 Fragments arranged by size: 2277 2120 1148 677 245 230 90 30 NaeI GCC'GGC Cuts at: 2070 3481 2070 Size: 1411 5406 NarI GG'CG_CC Cuts at: 2066 2066 Size: 6817 NcoI C'CATG_G Cuts at: 513 1065 2004 3828 4068 513 Size: 552 939 1824 240 3262 Fragments arranged by size: 3262 1824 939 552 240 NdeI CA'TA_TG Cuts at: 387 387 Size: 6817 NgoMIV G'CCGG_C Cuts at: 2068 3479 2068 Size: 1411 5406 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6817 NotI GC'GGCC_GC Cuts at: 4264 4264 Size: 6817 NspI r_CATG'y Cuts at: 1132 1229 3475 3877 4175 5720 1132 Size: 97 2246 402 298 1545 2229 Fragments arranged by size: 2246 2229 1545 402 298 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6817 PciI A'CATG_T Cuts at: 5716 5716 Size: 6817 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6817 PfoI T'CCnGG_A Cuts at: 2472 2553 3246 2472 Size: 81 693 6043 Fragments arranged by size: 6043 693 81 PmeI GTTT'AAAC Cuts at: 4121 4121 Size: 6817 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4886 4918 6399 6431 4886 Size: 32 1481 32 5272 Fragments arranged by size: 5272 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3705 3705 Size: 6817 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6817 PsiI TTA'TAA Cuts at: 4387 4387 Size: 6817 PspOMI G'GGCC_C Cuts at: 2134 2134 Size: 6817 PstI C_TGCA'G Cuts at: 830 2568 3222 3921 4169 830 Size: 1738 654 699 248 3478 Fragments arranged by size: 3478 1738 699 654 248 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6817 PvuII CAG'CTG Cuts at: 3883 3973 4003 3883 Size: 90 30 6697 Fragments arranged by size: 6697 90 30 SacI G_AGCT'C Cuts at: 3606 3750 3606 Size: 144 6673 SalI G'TCGA_C Cuts at: 1936 4159 1936 Size: 2223 4594 SapI GCTCTTCn'nnn_ Cuts at: 2496 2496 Size: 6817 SbfI CC_TGCA'GG Cuts at: 4169 4169 Size: 6817 ScaI AGT'ACT Cuts at: 5065 6777 5065 Size: 1712 5105 SfcI C'TryA_G Cuts at: 826 1045 2564 3218 3917 4165 5300 5981 6172 Size: 219 1519 654 699 248 1135 681 191 Cuts at: 6172 826 Size: 1471 Fragments arranged by size: 1519 1471 1135 699 681 654 248 219 191 SfoI GGC'GCC Cuts at: 2067 2067 Size: 6817 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6817 SgrAI Cr'CCGG_yG Cuts at: 1700 2068 1700 Size: 368 6449 SmaI CCC'GGG Cuts at: 1872 2676 2810 4144 1872 Size: 804 134 1334 4545 Fragments arranged by size: 4545 1334 804 134 SmlI C'TyrA_G Cuts at: 820 1017 1943 4921 5822 6084 820 Size: 197 926 2978 901 262 1553 Fragments arranged by size: 2978 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4608 493 Size: 4115 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6817 SphI G_CATG'C Cuts at: 1132 3475 3877 4175 1132 Size: 2343 402 298 3774 Fragments arranged by size: 3774 2343 402 298 SspI AAT'ATT Cuts at: 5 52 4741 5 Size: 47 4689 2081 Fragments arranged by size: 4689 2081 47 StuI AGG'CCT Cuts at: 1517 2549 1517 Size: 1032 5785 StyI C'CwwG_G Cuts at: 513 1065 2004 2373 2913 3126 3828 4061 4068 Size: 552 939 369 540 213 702 233 7 Cuts at: 4068 4256 513 Size: 188 3074 Fragments arranged by size: 3074 939 702 552 540 369 233 213 188 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3833 4844 4861 5014 5199 5644 325 Size: 1255 2253 1011 17 153 185 445 1498 Fragments arranged by size: 2253 1498 1255 1011 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2319 2832 2859 Size: 274 80 33 51 1443 342 513 27 Cuts at: 2859 3099 5063 6775 96 Size: 240 1964 1712 138 Fragments arranged by size: 1964 1712 1443 513 342 274 240 138 80 51 33 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2762 2794 6777 6809 2762 Size: 32 3983 32 2770 Fragments arranged by size: 3983 2770 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3665 1316 Size: 2349 4468 XbaI T'CTAG_A Cuts at: 4153 4153 Size: 6817 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2128 2371 2902 3520 2128 Size: 243 531 618 5425 Fragments arranged by size: 5425 618 531 243 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6817 XmaI C'CCGG_G Cuts at: 1870 2674 2808 4142 1870 Size: 804 134 1334 4545 Fragments arranged by size: 4545 1334 804 134 XmnI GAAnn'nnTTC Cuts at: 4946 4946 Size: 6817 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4625 276 Size: 53 83 186 936 3091 2468 Fragments arranged by size: 3091 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI