(Circular) (Six-base) MAPSORT of: pFN21AB9970.seq Check: 2302 from: 1 to: 6814 pFN21AB9970 6814 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3100 3100 Size: 6814 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4624 278 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3375 4157 1937 Size: 1438 782 4594 Fragments arranged by size: 4594 1438 782 Acc65I G'GTAC_C Cuts at: 4135 4135 Size: 6814 AclI AA'CG_TT Cuts at: 1563 4941 5314 1563 Size: 3378 373 3063 Fragments arranged by size: 3378 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3861 4046 4877 6261 6529 1632 Size: 2229 185 831 1384 268 1917 Fragments arranged by size: 2229 1917 1384 831 268 185 AfeI AGC'GCT Cuts at: 3218 3218 Size: 6814 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6617 AflIII A'CryG_T Cuts at: 1203 3000 5713 6690 1203 Size: 1797 2713 977 1327 Fragments arranged by size: 2713 1797 1327 977 AgeI A'CCGG_T Cuts at: 5645 5645 Size: 6814 AhdI GACnn_n'nnGTC Cuts at: 5543 5543 Size: 6814 AleI CACnn'nnGTG Cuts at: 1166 2318 3767 1166 Size: 1152 1449 4213 Fragments arranged by size: 4213 1449 1152 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2210 1184 Size: 1026 5788 AlwNI CAG_nnn'CTG Cuts at: 2626 2745 3351 4557 6129 2626 Size: 119 606 1206 1572 3311 Fragments arranged by size: 3311 1572 1206 606 119 ApaI G_GGCC'C Cuts at: 2559 4010 2559 Size: 1451 5363 ApaLI G'TGCA_C Cuts at: 3782 4871 6027 3782 Size: 1089 1156 4569 Fragments arranged by size: 4569 1156 1089 ApoI r'AATT_y Cuts at: 1493 4123 4349 4501 6610 1493 Size: 2630 226 152 2109 1697 Fragments arranged by size: 2630 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5368 160 Size: 872 4336 1606 Fragments arranged by size: 4336 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 AvaI C'yCGr_G Cuts at: 1870 1943 3224 4139 1870 Size: 73 1281 915 4545 Fragments arranged by size: 4545 1281 915 73 BamHI G'GATC_C Cuts at: 2855 4144 4511 2855 Size: 1289 367 5158 Fragments arranged by size: 5158 1289 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3228 4135 5590 618 Size: 325 217 627 1441 907 1455 1842 Fragments arranged by size: 1842 1455 1441 907 627 325 217 BanII G_rGCy'C Cuts at: 1391 2559 2720 2766 2996 3102 3266 4010 4133 Size: 1168 161 46 230 106 164 744 123 Cuts at: 4133 1391 Size: 4072 Fragments arranged by size: 4072 1168 744 230 164 161 123 106 46 BbeI G_GCGC'C Cuts at: 3232 3232 Size: 6814 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2413 3330 3450 3722 928 Size: 971 514 917 120 272 4020 Fragments arranged by size: 4020 971 917 514 272 120 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5005 5039 1903 Size: 34 3068 34 3678 Fragments arranged by size: 3678 3068 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4706 5916 4706 Size: 1210 5604 BclI T'GATC_A Cuts at: 1548 1548 Size: 6814 BfrBI ATG'CAT Cuts at: 2664 2979 2664 Size: 315 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2550 3237 3405 Size: 107 122 71 631 605 878 687 168 Cuts at: 3405 3890 5425 136 Size: 485 1535 1525 Fragments arranged by size: 1535 1525 878 687 631 605 485 168 122 107 71 BglII A'GATC_T Cuts at: 6809 6809 Size: 6814 BlpI GC'TnA_GC Cuts at: 3096 3416 4231 3096 Size: 320 815 5679 Fragments arranged by size: 5679 815 320 Bme1580I G_kGCm'C Cuts at: 1790 2559 2956 2963 3021 3674 3786 4010 4875 Size: 769 397 7 58 653 112 224 865 Cuts at: 4875 6031 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 865 769 653 397 224 112 58 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6814 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4545 5503 442 Size: 416 53 83 399 24 3128 958 1753 Fragments arranged by size: 3128 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6814 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2123 2243 2327 3689 4052 5474 1239 Size: 884 120 84 1362 363 1422 2579 Fragments arranged by size: 2579 1422 1362 884 363 120 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4939 5804 6102 4939 Size: 865 298 5651 Fragments arranged by size: 5651 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2444 3617 4066 5477 882 Size: 617 945 1173 449 1411 2219 Fragments arranged by size: 2219 1411 1173 945 617 449 BsaAI yAC'GTr Cuts at: 493 1204 3003 4605 493 Size: 711 1799 1602 2702 Fragments arranged by size: 2702 1799 1602 711 BsaBI GATnn'nnATC Cuts at: 6808 6808 Size: 6814 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3229 4621 5003 5638 Size: 53 83 186 936 1696 1392 382 635 Cuts at: 5638 275 Size: 1451 Fragments arranged by size: 1696 1451 1392 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2127 2453 5247 5645 5919 6066 6477 2127 Size: 326 2794 398 274 147 411 2464 Fragments arranged by size: 2794 2464 411 398 326 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3470 3500 3596 3626 3674 Size: 30 165 30 2067 30 96 30 48 Cuts at: 3674 3704 3776 3806 1178 Size: 30 72 30 4186 Fragments arranged by size: 4186 2067 165 96 72 48 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2240 2522 2562 3065 1186 Size: 1054 282 40 503 4935 Fragments arranged by size: 4935 1054 503 282 40 BseYI C'CCAG_C Cuts at: 2347 3259 6017 6513 2347 Size: 912 2758 496 2648 Fragments arranged by size: 2758 2648 912 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2216 3358 3544 1163 Size: 573 480 1142 186 4433 Fragments arranged by size: 4433 1142 573 480 186 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2079 4264 5025 6053 1540 Size: 275 186 78 2185 761 1028 2301 Fragments arranged by size: 2301 2185 1028 761 275 186 78 BsiHKAI G_wGCw'C Cuts at: 2766 3786 4875 4960 6031 2766 Size: 1020 1089 85 1071 3549 Fragments arranged by size: 3549 1089 1071 1020 85 BsmI GAATG_Cn' Cuts at: 2189 2666 3158 4325 4418 2189 Size: 477 492 1167 93 4585 Fragments arranged by size: 4585 1167 492 477 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4102 4102 Size: 6814 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2559 2720 2766 2956 2963 2996 3021 Size: 399 769 161 46 190 7 33 25 Cuts at: 3021 3102 3266 3674 3786 4010 4133 4875 4960 Size: 81 164 408 112 224 123 742 85 Cuts at: 4960 6031 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 769 742 408 399 224 190 164 161 123 112 85 81 46 33 25 7 BspEI T'CCGG_A Cuts at: 6477 6477 Size: 6814 BspHI T'CATG_A Cuts at: 4703 6433 4703 Size: 1730 5084 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3100 3303 3635 3933 4169 844 Size: 2256 203 332 298 236 3489 Fragments arranged by size: 3489 2256 332 298 236 203 BsrBI CCG'CTC Cuts at: 1394 1823 3690 4701 1394 Size: 429 1867 1011 3507 Fragments arranged by size: 3507 1867 1011 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2335 5309 5483 65 Size: 1183 1087 2974 174 1396 Fragments arranged by size: 2974 1396 1183 1087 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2685 3399 5458 5645 1700 Size: 110 875 714 2059 187 2869 Fragments arranged by size: 2869 2059 875 714 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6814 BssHII G'CGCG_C Cuts at: 1925 2011 3084 1925 Size: 86 1073 5655 Fragments arranged by size: 5655 1073 86 BssSI C'ACGA_G Cuts at: 4874 5886 4874 Size: 1012 5802 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6814 BstBI TT'CG_AA Cuts at: 5618 5618 Size: 6814 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6814 BstYI r'GATC_y Cuts at: 1972 2855 4144 4511 4896 4913 6365 6451 6463 Size: 883 1289 367 385 17 1452 86 12 Cuts at: 6463 6809 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1289 883 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2241 2287 3031 3285 3972 513 Size: 552 939 237 46 744 254 687 3355 Fragments arranged by size: 3355 939 744 687 552 254 237 46 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3101 3435 487 Size: 683 56 1875 334 3866 Fragments arranged by size: 3866 1875 683 334 56 BtsI GCAGTG_nn' Cuts at: 2210 2576 4330 5124 5144 2210 Size: 366 1754 794 20 3880 Fragments arranged by size: 3880 1754 794 366 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6814 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6779 DraI TTT'AAA Cuts at: 4118 4118 Size: 6814 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2093 2594 5821 809 Size: 507 777 501 3227 1802 Fragments arranged by size: 3227 1802 777 507 501 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2133 2256 Size: 54 1425 50 275 18 246 57 123 Cuts at: 2256 3028 3238 3562 4261 5150 6779 8 Size: 772 210 324 699 889 1629 43 Fragments arranged by size: 1629 1425 889 772 699 324 275 246 210 123 57 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2076 4261 1537 Size: 275 264 2185 4090 Fragments arranged by size: 4090 2185 275 264 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 3837 4744 1353 Size: 965 1519 907 3423 Fragments arranged by size: 3423 1519 965 907 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5401 5775 5921 387 Size: 1064 138 3812 374 146 1280 Fragments arranged by size: 3812 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2764 2764 Size: 6814 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2243 2327 3689 3861 4046 4052 Size: 393 491 120 84 1362 172 185 6 Cuts at: 4052 4877 5474 6261 6529 1239 Size: 825 597 787 268 1524 Fragments arranged by size: 1524 1362 825 787 597 491 393 268 185 172 120 84 6 EcoNI CCTnn'n_nnAGG Cuts at: 2102 2102 Size: 6814 EcoO109I rG'GnC_Cy Cuts at: 2032 2473 2521 2556 2032 Size: 441 48 35 6290 Fragments arranged by size: 6290 441 48 35 EcoRI G'AATT_C Cuts at: 4123 4501 4123 Size: 378 6436 EcoRV GAT'ATC Cuts at: 2929 2929 Size: 6814 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2911 2911 Size: 6814 FspI TGC'GCA Cuts at: 5320 5320 Size: 6814 HaeII r_GCGC'y Cuts at: 1678 3220 3232 5961 1678 Size: 1542 12 2729 2531 Fragments arranged by size: 2729 2531 1542 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2122 2154 2624 2656 3299 Size: 32 135 32 550 32 470 32 643 Cuts at: 3299 3331 5455 5487 5529 5561 1373 Size: 32 2124 32 42 32 2626 Fragments arranged by size: 2626 2124 643 550 470 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3205 4081 4158 4404 669 Size: 988 281 1267 876 77 246 3079 Fragments arranged by size: 3079 1267 988 876 281 246 77 HindIII A'AGCT_T Cuts at: 748 3514 748 Size: 2766 4048 HpaI GTT'AAC Cuts at: 4404 4404 Size: 6814 KasI G'GCGC_C Cuts at: 3228 3228 Size: 6814 KpnI G_GTAC'C Cuts at: 4139 4139 Size: 6814 MfeI C'AATT_G Cuts at: 4413 4413 Size: 6814 MluI A'CGCG_T Cuts at: 6690 6690 Size: 6814 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5928 6112 1305 Size: 4623 184 2007 Fragments arranged by size: 4623 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2258 3240 10 Size: 54 1425 343 303 123 982 3584 Fragments arranged by size: 3584 1425 982 343 303 123 54 MslI CAynn'nnrTG Cuts at: 518 1166 2318 3767 3971 4071 4772 5131 5290 Size: 648 1152 1449 204 100 701 359 159 Cuts at: 5290 518 Size: 2042 Fragments arranged by size: 2042 1449 1152 701 648 359 204 159 100 MspA1I CmG'CkG Cuts at: 1606 2044 2221 2274 2541 2623 2682 2727 2734 Size: 438 177 53 267 82 59 45 7 Cuts at: 2734 2745 2865 3147 3279 3495 3528 3654 3732 Size: 11 120 282 132 216 33 126 78 Cuts at: 3732 4230 4907 6055 6300 1606 Size: 498 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 498 438 282 267 245 216 177 132 126 120 82 78 59 53 45 33 11 7 NaeI GCC'GGC Cuts at: 2687 3401 2687 Size: 714 6100 NarI GG'CG_CC Cuts at: 3229 3229 Size: 6814 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5323 Fragments arranged by size: 5323 939 552 NdeI CA'TA_TG Cuts at: 387 2164 387 Size: 1777 5037 NgoMIV G'CCGG_C Cuts at: 2685 3399 2685 Size: 714 6100 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6814 NotI GC'GGCC_GC Cuts at: 4261 4261 Size: 6814 NsiI A_TGCA'T Cuts at: 2666 2981 2666 Size: 315 6499 NspI r_CATG'y Cuts at: 1132 1229 2433 2979 3768 4172 5717 1132 Size: 97 1204 546 789 404 1545 2229 Fragments arranged by size: 2229 1545 1204 789 546 404 97 PasI CC'CwG_GG Cuts at: 1348 2368 1348 Size: 1020 5794 PciI A'CATG_T Cuts at: 5713 5713 Size: 6814 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6814 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 6814 PmeI GTTT'AAAC Cuts at: 4118 4118 Size: 6814 PmlI CAC'GTG Cuts at: 3003 3003 Size: 6814 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4094 4126 4883 4915 6396 6428 4094 Size: 32 757 32 1481 32 4480 Fragments arranged by size: 4480 1481 757 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2521 2032 Size: 489 6325 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6814 PsiI TTA'TAA Cuts at: 4384 4384 Size: 6814 PspOMI G'GGCC_C Cuts at: 2555 4006 2555 Size: 1451 5363 PstI C_TGCA'G Cuts at: 830 3278 4166 830 Size: 2448 888 3478 Fragments arranged by size: 3478 2448 888 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6814 PvuII CAG'CTG Cuts at: 2221 2623 2682 2745 3279 3528 2221 Size: 402 59 63 534 249 5507 Fragments arranged by size: 5507 534 402 249 63 59 RsrII CG'GwC_CG Cuts at: 3727 3727 Size: 6814 SacI G_AGCT'C Cuts at: 2766 2766 Size: 6814 SalI G'TCGA_C Cuts at: 1936 4156 1936 Size: 2220 4594 SanDI GG'GwC_CC Cuts at: 2032 2032 Size: 6814 SbfI CC_TGCA'GG Cuts at: 4166 4166 Size: 6814 ScaI AGT'ACT Cuts at: 4106 5062 6774 4106 Size: 956 1712 4146 Fragments arranged by size: 4146 1712 956 SfcI C'TryA_G Cuts at: 826 1045 2879 3274 3352 3544 4162 5297 5978 Size: 219 1834 395 78 192 618 1135 681 Cuts at: 5978 6169 826 Size: 191 1471 Fragments arranged by size: 1834 1471 1135 681 618 395 219 192 191 78 SfoI GGC'GCC Cuts at: 3230 3230 Size: 6814 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6814 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6814 SmaI CCC'GGG Cuts at: 1872 3226 4141 1872 Size: 1354 915 4545 Fragments arranged by size: 4545 1354 915 SmlI C'TyrA_G Cuts at: 820 1017 1943 4918 5819 6081 820 Size: 197 926 2975 901 262 1553 Fragments arranged by size: 2975 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4605 493 Size: 4112 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6814 SphI G_CATG'C Cuts at: 1132 2433 4172 1132 Size: 1301 1739 3774 Fragments arranged by size: 3774 1739 1301 SspI AAT'ATT Cuts at: 5 52 4738 5 Size: 47 4686 2081 Fragments arranged by size: 4686 2081 47 StuI AGG'CCT Cuts at: 1517 2108 1517 Size: 591 6223 StyI C'CwwG_G Cuts at: 513 1065 2004 4253 513 Size: 552 939 2249 3074 Fragments arranged by size: 3074 2249 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3965 4841 4858 5011 5196 5641 325 Size: 1255 2385 876 17 153 185 445 1498 Fragments arranged by size: 2385 1498 1255 876 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2385 4104 5060 Size: 274 80 33 51 1443 408 1719 956 Cuts at: 5060 6772 96 Size: 1712 138 Fragments arranged by size: 1719 1712 1443 956 408 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3470 3502 3596 3628 3776 3808 6774 6806 3470 Size: 32 94 32 148 32 2966 32 3478 Fragments arranged by size: 3478 2966 148 94 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3725 1316 Size: 2409 4405 XbaI T'CTAG_A Cuts at: 4150 4150 Size: 6814 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6814 XmaI C'CCGG_G Cuts at: 1870 3224 4139 1870 Size: 1354 915 4545 Fragments arranged by size: 4545 1354 915 XmnI GAAnn'nnTTC Cuts at: 4943 4943 Size: 6814 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4622 276 Size: 53 83 186 936 3088 2468 Fragments arranged by size: 3088 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII FseI FspAI NruI PacI PspXI PsrI SacII SapI SexAI SfiI SrfI SwaI XcmI