(Circular) (Six-base) MAPSORT of: pFN21AB9965.seq Check: 8770 from: 1 to: 6352 pFN21AB9965 6352 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4162 278 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2303 3695 1937 Size: 366 1392 4594 Fragments arranged by size: 4594 1392 366 Acc65I G'GTAC_C Cuts at: 2204 3673 2204 Size: 1469 4883 AclI AA'CG_TT Cuts at: 1563 4479 4852 1563 Size: 2916 373 3063 Fragments arranged by size: 3063 2916 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2400 2810 3441 4415 5799 6067 1632 Size: 768 410 631 974 1384 268 1917 Fragments arranged by size: 1917 1384 974 768 631 410 268 AfeI AGC'GCT Cuts at: 3140 3140 Size: 6352 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6155 AflIII A'CryG_T Cuts at: 1203 5251 6228 1203 Size: 4048 977 1327 Fragments arranged by size: 4048 1327 977 AgeI A'CCGG_T Cuts at: 5183 5183 Size: 6352 AhdI GACnn_n'nnGTC Cuts at: 5081 5081 Size: 6352 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6352 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3159 3309 1184 Size: 1975 150 4227 Fragments arranged by size: 4227 1975 150 AlwNI CAG_nnn'CTG Cuts at: 4095 5667 4095 Size: 1572 4780 ApaLI G'TGCA_C Cuts at: 3144 4409 5565 3144 Size: 1265 1156 3931 Fragments arranged by size: 3931 1265 1156 ApoI r'AATT_y Cuts at: 1493 2399 2456 2793 3262 3410 3661 3887 4039 Size: 906 57 337 469 148 251 226 152 Cuts at: 4039 6148 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 906 469 337 251 226 152 148 57 AseI AT'TA_AT Cuts at: 160 1032 4906 160 Size: 872 3874 1606 Fragments arranged by size: 3874 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 AvaI C'yCGr_G Cuts at: 1870 1943 3677 1870 Size: 73 1734 4545 Fragments arranged by size: 4545 1734 73 BamHI G'GATC_C Cuts at: 3682 4049 3682 Size: 367 5985 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2084 2204 3673 5128 618 Size: 325 217 627 297 120 1469 1455 1842 Fragments arranged by size: 1842 1469 1455 627 325 297 217 120 BanII G_rGCy'C Cuts at: 1391 3671 1391 Size: 2280 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3368 3467 928 Size: 971 1469 99 3813 Fragments arranged by size: 3813 1469 971 99 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3209 3243 4543 4577 1903 Size: 34 1272 34 1300 34 3678 Fragments arranged by size: 3678 1300 1272 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4244 5454 4244 Size: 1210 5142 BclI T'GATC_A Cuts at: 1548 1548 Size: 6352 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2100 4963 136 Size: 107 122 71 631 605 428 2863 1525 Fragments arranged by size: 2863 1525 631 605 428 122 107 71 BglII A'GATC_T Cuts at: 6347 6347 Size: 6352 BlpI GC'TnA_GC Cuts at: 2438 2730 3769 2438 Size: 292 1039 5021 Fragments arranged by size: 5021 1039 292 Bme1580I G_kGCm'C Cuts at: 1790 2087 2499 3148 4413 5569 1790 Size: 297 412 649 1265 1156 2573 Fragments arranged by size: 2573 1265 1156 649 412 297 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6352 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2210 2555 3390 Size: 416 53 83 399 24 793 345 835 Cuts at: 3390 4083 5041 442 Size: 693 958 1753 Fragments arranged by size: 1753 958 835 793 693 416 399 345 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6352 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3011 5012 1239 Size: 1772 2001 2579 Fragments arranged by size: 2579 2001 1772 Bpu10I CC'TnA_GC Cuts at: 2391 2391 Size: 6352 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4477 5342 5640 4477 Size: 865 298 5189 Fragments arranged by size: 5189 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5015 882 Size: 617 3516 2219 Fragments arranged by size: 3516 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4143 493 Size: 711 2939 2702 Fragments arranged by size: 2939 2702 711 BsaBI GATnn'nnATC Cuts at: 6346 6346 Size: 6352 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2172 4159 4541 5176 Size: 53 83 186 936 639 1987 382 635 Cuts at: 5176 275 Size: 1451 Fragments arranged by size: 1987 1451 936 639 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4785 5183 5457 5604 6015 4785 Size: 398 274 147 411 5122 Fragments arranged by size: 5122 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2132 2162 1178 Size: 30 165 30 729 30 5368 Fragments arranged by size: 5368 729 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2144 2156 2653 1186 Size: 958 12 497 4885 Fragments arranged by size: 4885 958 497 12 BseYI C'CCAG_C Cuts at: 5555 6051 5555 Size: 496 5856 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2039 1163 Size: 573 303 5476 Fragments arranged by size: 5476 573 303 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3802 4563 5591 1540 Size: 275 186 1801 761 1028 2301 Fragments arranged by size: 2301 1801 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3148 4413 4498 5569 3148 Size: 1265 85 1071 3931 Fragments arranged by size: 3931 1265 1071 85 BsmI GAATG_Cn' Cuts at: 2273 2377 3863 3956 2273 Size: 104 1486 93 4669 Fragments arranged by size: 4669 1486 104 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2087 2499 3148 3671 4413 4498 5569 Size: 399 297 412 649 523 742 85 1071 Cuts at: 5569 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 649 523 412 399 297 85 BspEI T'CCGG_A Cuts at: 6015 6015 Size: 6352 BspHI T'CATG_A Cuts at: 4241 5971 4241 Size: 1730 4622 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3707 844 Size: 2863 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4239 1394 Size: 429 2416 3507 Fragments arranged by size: 3507 2416 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4847 5021 65 Size: 1183 3599 174 1396 Fragments arranged by size: 3599 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2058 4996 5183 1700 Size: 110 248 2938 187 2869 Fragments arranged by size: 2938 2869 248 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6352 BssSI C'ACGA_G Cuts at: 4412 5424 4412 Size: 1012 5340 BstAPI GCAn_nnn'nTGC Cuts at: 794 2091 794 Size: 1297 5055 BstBI TT'CG_AA Cuts at: 2233 3601 5156 2233 Size: 1368 1555 3429 Fragments arranged by size: 3429 1555 1368 BstEII G'GTnAC_C Cuts at: 1184 3031 3122 1184 Size: 1847 91 4414 Fragments arranged by size: 4414 1847 91 BstYI r'GATC_y Cuts at: 1972 3222 3512 3682 4049 4434 4451 5903 5989 Size: 1250 290 170 367 385 17 1452 86 Cuts at: 5989 6001 6347 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1250 385 367 346 290 170 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2678 2678 Size: 6352 BtgI C'CryG_G Cuts at: 513 1065 2004 2033 513 Size: 552 939 29 4832 Fragments arranged by size: 4832 939 552 29 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5613 Fragments arranged by size: 5613 683 56 BtsI GCAGTG_nn' Cuts at: 3868 4662 4682 3868 Size: 794 20 5538 Fragments arranged by size: 5538 794 20 ClaI AT'CG_AT Cuts at: 1554 2508 1554 Size: 954 5398 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2734 2769 529 Size: 35 2170 35 4112 Fragments arranged by size: 4112 2170 35 35 DraI TTT'AAA Cuts at: 3656 3656 Size: 6352 DraIII CAC_nnn'GTG Cuts at: 2091 2091 Size: 6352 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5359 809 Size: 507 4043 1802 Fragments arranged by size: 4043 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317 Size: 54 1425 50 275 18 1969 889 1629 Cuts at: 6317 8 Size: 43 Fragments arranged by size: 1969 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3799 1537 Size: 275 1987 4090 Fragments arranged by size: 4090 1987 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2526 3206 4282 1353 Size: 1173 680 1076 3423 Fragments arranged by size: 3423 1173 1076 680 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4939 5313 5459 387 Size: 1064 138 3350 374 146 1280 Fragments arranged by size: 3350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2400 2810 3011 3441 4415 5012 5799 Size: 393 768 410 201 430 974 597 787 Cuts at: 5799 6067 1239 Size: 268 1524 Fragments arranged by size: 1524 974 787 768 597 430 410 393 268 201 EcoO109I rG'GnC_Cy Cuts at: 3091 3091 Size: 6352 EcoRI G'AATT_C Cuts at: 2793 3661 4039 2793 Size: 868 378 5106 Fragments arranged by size: 5106 868 378 FspI TGC'GCA Cuts at: 4858 4858 Size: 6352 HaeII r_GCGC'y Cuts at: 1678 2557 3142 5499 1678 Size: 879 585 2357 2531 Fragments arranged by size: 2531 2357 879 585 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2285 2317 2699 2731 3214 Size: 32 135 32 713 32 382 32 483 Cuts at: 3214 3246 4993 5025 5067 5099 1373 Size: 32 1747 32 42 32 2626 Fragments arranged by size: 2626 1747 713 483 382 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3553 3696 3942 669 Size: 988 281 1615 143 246 3079 Fragments arranged by size: 3079 1615 988 281 246 143 HindIII A'AGCT_T Cuts at: 748 2853 748 Size: 2105 4247 HpaI GTT'AAC Cuts at: 3553 3942 3553 Size: 389 5963 KpnI G_GTAC'C Cuts at: 2208 3677 2208 Size: 1469 4883 MfeI C'AATT_G Cuts at: 2484 3951 2484 Size: 1467 4885 MluI A'CGCG_T Cuts at: 6228 6228 Size: 6352 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 3485 5466 5650 1305 Size: 921 1259 1981 184 2007 Fragments arranged by size: 2007 1981 1259 921 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4530 Fragments arranged by size: 4530 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2324 3291 4310 4669 4828 518 Size: 648 1158 967 1019 359 159 2042 Fragments arranged by size: 2042 1158 1019 967 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2035 3768 4445 5593 5838 1606 Size: 429 1733 677 1148 245 2120 Fragments arranged by size: 2120 1733 1148 677 429 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4861 Fragments arranged by size: 4861 939 552 NdeI CA'TA_TG Cuts at: 387 3538 387 Size: 3151 3201 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6352 NotI GC'GGCC_GC Cuts at: 3799 3799 Size: 6352 NspI r_CATG'y Cuts at: 1132 1229 3290 3710 5255 1132 Size: 97 2061 420 1545 2229 Fragments arranged by size: 2229 2061 1545 420 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6352 PciI A'CATG_T Cuts at: 5251 5251 Size: 6352 PflMI CCAn_nnn'nTGG Cuts at: 1108 2430 2624 1108 Size: 1322 194 4836 Fragments arranged by size: 4836 1322 194 PfoI T'CCnGG_A Cuts at: 2254 2254 Size: 6352 PmeI GTTT'AAAC Cuts at: 3656 3656 Size: 6352 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4421 4453 5934 5966 4421 Size: 32 1481 32 4807 Fragments arranged by size: 4807 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6352 PsiI TTA'TAA Cuts at: 3922 3922 Size: 6352 PstI C_TGCA'G Cuts at: 830 3117 3704 830 Size: 2287 587 3478 Fragments arranged by size: 3478 2287 587 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6352 SacII CC_GC'GG Cuts at: 2036 2036 Size: 6352 SalI G'TCGA_C Cuts at: 1936 3694 1936 Size: 1758 4594 SapI GCTCTTCn'nnn_ Cuts at: 2526 2526 Size: 6352 SbfI CC_TGCA'GG Cuts at: 3704 3704 Size: 6352 ScaI AGT'ACT Cuts at: 2565 4600 6312 2565 Size: 2035 1712 2605 Fragments arranged by size: 2605 2035 1712 SfcI C'TryA_G Cuts at: 826 1045 3113 3353 3700 4835 5516 5707 826 Size: 219 2068 240 347 1135 681 191 1471 Fragments arranged by size: 2068 1471 1135 681 347 240 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6352 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6352 SmaI CCC'GGG Cuts at: 1872 3679 1872 Size: 1807 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4456 5357 5619 820 Size: 197 926 2513 901 262 1553 Fragments arranged by size: 2513 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4143 493 Size: 3650 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6352 SphI G_CATG'C Cuts at: 1132 3290 3710 1132 Size: 2158 420 3774 Fragments arranged by size: 3774 2158 420 SspI AAT'ATT Cuts at: 5 52 2991 4276 5 Size: 47 2939 1285 2081 Fragments arranged by size: 2939 2081 1285 47 StuI AGG'CCT Cuts at: 1517 2906 1517 Size: 1389 4963 StyI C'CwwG_G Cuts at: 513 1065 2004 3039 3791 513 Size: 552 939 1035 752 3074 Fragments arranged by size: 3074 1035 939 752 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2898 3141 4379 4396 4549 4734 5179 Size: 1255 1318 243 1238 17 153 185 445 Cuts at: 5179 325 Size: 1498 Fragments arranged by size: 1498 1318 1255 1238 445 243 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2563 4598 6310 Size: 274 80 33 51 1443 586 2035 1712 Cuts at: 6310 96 Size: 138 Fragments arranged by size: 2035 1712 1443 586 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2132 2164 3064 3096 3200 3232 6312 6344 2132 Size: 32 900 32 104 32 3080 32 2140 Fragments arranged by size: 3080 2140 900 104 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3367 1316 Size: 2051 4301 XbaI T'CTAG_A Cuts at: 3688 3688 Size: 6352 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2585 2585 Size: 6352 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6352 XmaI C'CCGG_G Cuts at: 1870 3677 1870 Size: 1807 4545 XmnI GAAnn'nnTTC Cuts at: 2520 3016 4481 2520 Size: 496 1465 4391 Fragments arranged by size: 4391 1465 496 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4160 276 Size: 53 83 186 936 2626 2468 Fragments arranged by size: 2626 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI