Restriction map for
pFN21AB9960
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaHI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI BspDI BspEI
BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstUI
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoT14I FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I StuI StyI SwaI Tth111I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AccI 871 GTATAC GT^[AC][GT]AC
AflIII 512 ACATGT A^C[GA][CT]GT
AlwNI 52 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 484 GAGCTC G[AT]GC[AT]^C
BciVI 240 GGATAC GTATCC(6/5)
BglII 1281 AGATCT A^GATCT
BsaAI 1070 TACGTG [CT]AC^GT[GA]
BsaBI 1351 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1148 GAGACC GGTCTC(1/5)
BsaWI 1321 ACCGGA [AT]^CCGG[AT]
BsiHKAI 484 GAGCTC G[AT]GC[AT]^C
BsmFI 1610 GGGAC GGGAC(10/14)
Bsp1407I 833 TGTACA T^GTACA
BspLU11I 512 ACATGT A^CATGT
BsrGI 833 TGTACA T^GTACA
Bst1107I 871 GTATAC GTA^TAC
BstEII 595 GGTGACC G^GT[ACGT]ACC
BstPI 595 GGTGACC G^GT[ACGT]ACC
BstXI 1299 CCAAGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 871 GTATAC GTA^TAC
EcoNI 126 CCTCTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 595 GGTGACC G^GT[ACGT]ACC
EcoRV 1660 GATATC GAT^ATC
EcoT22I 884 ATGCAT ATGCA^T
MaeII 1071 ACGT A^CGT
MamI 1351 GATCAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NdeI 1263 CATATG CA^TATG
NsiI 884 ATGCAT ATGCA^T
NspI 512 ACATGT [GA]CATG^[CT]
PflMI 1491 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 857 GACTC GAGTC(4/5)
PstI 99 CTGCAG CTGCA^G
SacI 484 GAGCTC GAGCT^C
ScaI 428 AGTACT AGT^ACT
SfcI 99 CTGCAG C^T[GA][CT]AG
SspBI 833 TGTACA T^GTACA
SspI 377 AATATT AAT^ATT
TaqI 1385 TCGA T^CGA
TthHB8I 1385 TCGA T^CGA
Van91I 1491 CCAAGCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1284 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AseI 361 ATTAAT AT^TAAT
1197 ATTAAT
AsnI 361 ATTAAT AT^TAAT
1197 ATTAAT
Asp700I 737 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1519 GAAGTGATTC
BanII 484 GAGCTC G[GA]GC[CT]^C
604 GAGCCC
BcnI 335 CCGGG CC^[GC]GG
1227 CCCGG
BmyI 484 GAGCTC G[AGT]GC[ACT]^C
604 GAGCCC
BpmI 949 CTGGAG CTGGAG(16/14)
1145 CTGGAG
Bsp1286I 484 GAGCTC G[AGT]GC[ACT]^C
604 GAGCCC
BstYI 1281 AGATCT [GA]^GATC[CT]
1589 AGATCC
DraI 1155 TTTAAA TTT^AAA
1314 TTTAAA
EarI 655 GAAGAG CTCTTC(1/4)
1585 GAAGAG
FauI 69 GCGGG CCCGC(4/6)
73 GCGGG
Ksp632I 655 GAAGAG CTCTTC(1/4)
1585 GAAGAG
MflI 1281 AGATCT [GA]^GATC[CT]
1589 AGATCC
MslI 246 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
862 CATGCTGATG
NciI 335 CCGGG CC^[GC]GG
1227 CCCGG
PpuMI 1223 AGGACCC [GA]G^G[AT]CC[CT]
1436 AGGACCT
PshBI 361 ATTAAT AT^TAAT
1197 ATTAAT
PvuII 96 CAGCTG CAG^CTG
248 CAGCTG
XhoII 1281 AGATCT [GA]^GATC[CT]
1589 AGATCC
XmnI 737 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1519 GAAGTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BalI BbvI BclI
BfaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MluNI MnlI MscI MseI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI