(Circular) (Six-base) MAPSORT of: pFN21AB9960.seq Check: 9808 from: 1 to: 6772 pFN21AB9960 6772 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4582 278 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2877 4115 1937 Size: 940 1238 4594 Fragments arranged by size: 4594 1238 940 Acc65I G'GTAC_C Cuts at: 4093 4093 Size: 6772 AclI AA'CG_TT Cuts at: 1563 4899 5272 1563 Size: 3336 373 3063 Fragments arranged by size: 3336 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2201 2495 2984 3438 3620 3898 4835 6219 Size: 569 294 489 454 182 278 937 1384 Cuts at: 6219 6487 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 937 569 489 454 294 278 268 182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6575 AflIII A'CryG_T Cuts at: 1203 2517 5671 6648 1203 Size: 1314 3154 977 1327 Fragments arranged by size: 3154 1327 1314 977 AgeI A'CCGG_T Cuts at: 5603 5603 Size: 6772 AhdI GACnn_n'nnGTC Cuts at: 5501 5501 Size: 6772 AleI CACnn'nnGTG Cuts at: 1166 2255 1166 Size: 1089 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2119 1184 Size: 935 5837 AlwNI CAG_nnn'CTG Cuts at: 2062 4515 6087 2062 Size: 2453 1572 2747 Fragments arranged by size: 2747 2453 1572 ApaLI G'TGCA_C Cuts at: 4829 5985 4829 Size: 1156 5616 ApoI r'AATT_y Cuts at: 1493 2927 3341 3537 4081 4307 4459 6568 1493 Size: 1434 414 196 544 226 152 2109 1697 Fragments arranged by size: 2109 1697 1434 544 414 226 196 152 AseI AT'TA_AT Cuts at: 160 1032 2367 3203 5326 160 Size: 872 1335 836 2123 1606 Fragments arranged by size: 2123 1606 1335 872 836 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 AvaI C'yCGr_G Cuts at: 1870 1943 4097 1870 Size: 73 2154 4545 Fragments arranged by size: 4545 2154 73 BamHI G'GATC_C Cuts at: 4102 4469 4102 Size: 367 6405 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4093 5548 618 Size: 325 217 627 2306 1455 1842 Fragments arranged by size: 2306 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2493 2613 4091 1391 Size: 1102 120 1478 4072 Fragments arranged by size: 4072 1478 1102 120 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4963 4997 1903 Size: 34 3026 34 3678 Fragments arranged by size: 3678 3026 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2239 4664 5874 2239 Size: 2425 1210 3137 Fragments arranged by size: 3137 2425 1210 BclI T'GATC_A Cuts at: 1548 2524 3142 3355 1548 Size: 976 618 213 4965 Fragments arranged by size: 4965 976 618 213 BfrBI ATG'CAT Cuts at: 2891 2891 Size: 6772 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5383 136 Size: 107 122 71 631 605 3711 1525 Fragments arranged by size: 3711 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3286 6767 3286 Size: 3481 3291 BlpI GC'TnA_GC Cuts at: 4189 4189 Size: 6772 Bme1580I G_kGCm'C Cuts at: 1790 4833 5989 1790 Size: 3043 1156 2573 Fragments arranged by size: 3043 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6772 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4503 5461 442 Size: 416 53 83 399 24 3086 958 1753 Fragments arranged by size: 3086 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6772 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3237 3237 Size: 6772 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2975 3171 5432 1239 Size: 1736 196 2261 2579 Fragments arranged by size: 2579 2261 1736 196 Bpu10I CC'TnA_GC Cuts at: 3708 3708 Size: 6772 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4897 5762 6060 4897 Size: 865 298 5609 Fragments arranged by size: 5609 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3147 5435 882 Size: 617 1648 2288 2219 Fragments arranged by size: 2288 2219 1648 617 BsaAI yAC'GTr Cuts at: 493 1204 3077 4563 493 Size: 711 1873 1486 2702 Fragments arranged by size: 2702 1873 1486 711 BsaBI GATnn'nnATC Cuts at: 3360 6766 3360 Size: 3406 3366 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4579 4961 5596 275 Size: 53 83 186 936 3046 382 635 1451 Fragments arranged by size: 3046 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3326 5205 5603 5877 6024 6435 3326 Size: 1879 398 274 147 411 3663 Fragments arranged by size: 3663 1879 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3477 3507 1178 Size: 30 165 30 2074 30 4443 Fragments arranged by size: 4443 2074 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6772 BseYI C'CCAG_C Cuts at: 2032 3761 5975 6471 2032 Size: 1729 2214 496 2333 Fragments arranged by size: 2333 2214 1729 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6199 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4222 4983 6011 1540 Size: 275 186 2221 761 1028 2301 Fragments arranged by size: 2301 2221 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 4833 4918 5989 2493 Size: 2340 85 1071 3276 Fragments arranged by size: 3276 2340 1071 85 BsmI GAATG_Cn' Cuts at: 4283 4376 4283 Size: 93 6679 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2493 2613 4091 4833 4918 5989 1391 Size: 399 703 120 1478 742 85 1071 2174 Fragments arranged by size: 2174 1478 1071 742 703 399 120 85 BspEI T'CCGG_A Cuts at: 6435 6435 Size: 6772 BspHI T'CATG_A Cuts at: 4661 6391 4661 Size: 1730 5042 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4127 844 Size: 3283 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4659 1394 Size: 429 2836 3507 Fragments arranged by size: 3507 2836 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5267 5441 65 Size: 1183 4019 174 1396 Fragments arranged by size: 4019 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5416 5603 1700 Size: 110 3606 187 2869 Fragments arranged by size: 3606 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2838 96 Size: 2742 4030 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6772 BssSI C'ACGA_G Cuts at: 4832 5844 4832 Size: 1012 5760 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6772 BstBI TT'CG_AA Cuts at: 5576 5576 Size: 6772 BstEII G'GTnAC_C Cuts at: 1184 2600 1184 Size: 1416 5356 BstXI CCAn_nnnn'nTGG Cuts at: 3311 3311 Size: 6772 BstYI r'GATC_y Cuts at: 1972 3286 3594 4102 4469 4854 4871 6323 6409 Size: 1314 308 508 367 385 17 1452 86 Cuts at: 6409 6421 6767 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1314 508 385 367 346 308 86 17 12 BstZ17I GTA'TAC Cuts at: 2878 2878 Size: 6772 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5281 Fragments arranged by size: 5281 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6033 Fragments arranged by size: 6033 683 56 BtsI GCAGTG_nn' Cuts at: 3583 4045 4288 5082 5102 3583 Size: 462 243 794 20 5253 Fragments arranged by size: 5253 794 462 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6772 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6737 DraI TTT'AAA Cuts at: 3162 3321 4076 3162 Size: 159 755 5858 Fragments arranged by size: 5858 755 159 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5779 809 Size: 507 4463 1802 Fragments arranged by size: 4463 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2352 3510 Size: 54 1425 50 275 18 223 299 1158 Cuts at: 3510 3716 4219 5108 6737 8 Size: 206 503 889 1629 43 Fragments arranged by size: 1629 1425 1158 889 503 299 275 223 206 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4219 1537 Size: 275 2407 4090 Fragments arranged by size: 4090 2407 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2655 3585 4702 1353 Size: 1302 930 1117 3423 Fragments arranged by size: 3423 1302 1117 930 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5359 5733 5879 387 Size: 1064 138 3770 374 146 1280 Fragments arranged by size: 3770 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2491 2491 Size: 6772 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2201 2495 2975 2984 3171 3438 3620 Size: 393 569 294 480 9 187 267 182 Cuts at: 3620 3898 4835 5432 6219 6487 1239 Size: 278 937 597 787 268 1524 Fragments arranged by size: 1524 937 787 597 569 480 393 294 278 268 267 187 182 9 EcoNI CCTnn'n_nnAGG Cuts at: 2135 2135 Size: 6772 EcoO109I rG'GnC_Cy Cuts at: 3229 3442 3934 3229 Size: 213 492 6067 Fragments arranged by size: 6067 492 213 EcoRI G'AATT_C Cuts at: 4081 4459 4081 Size: 378 6394 EcoRV GAT'ATC Cuts at: 3667 3667 Size: 6772 FspI TGC'GCA Cuts at: 5278 5278 Size: 6772 HaeII r_GCGC'y Cuts at: 1678 5919 1678 Size: 4241 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2663 2695 3579 3611 3656 Size: 32 135 32 1091 32 884 32 45 Cuts at: 3656 3688 5413 5445 5487 5519 1373 Size: 32 1725 32 42 32 2626 Fragments arranged by size: 2626 1725 1091 884 135 45 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4116 4362 669 Size: 988 281 2178 246 3079 Fragments arranged by size: 3079 2178 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6772 HpaI GTT'AAC Cuts at: 4362 4362 Size: 6772 KpnI G_GTAC'C Cuts at: 4097 4097 Size: 6772 MfeI C'AATT_G Cuts at: 4371 4371 Size: 6772 MluI A'CGCG_T Cuts at: 6648 6648 Size: 6772 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5886 6070 1305 Size: 4581 184 2007 Fragments arranged by size: 4581 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 2354 3512 3718 10 Size: 54 1425 343 223 299 1158 206 3064 Fragments arranged by size: 3064 1425 1158 343 299 223 206 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2871 4730 5089 5248 518 Size: 648 1089 616 1859 359 159 2042 Fragments arranged by size: 2042 1859 1089 648 616 359 159 MspA1I CmG'CkG Cuts at: 1606 2062 2103 2255 4188 4865 6013 6258 1606 Size: 456 41 152 1933 677 1148 245 2120 Fragments arranged by size: 2120 1933 1148 677 456 245 152 41 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5281 Fragments arranged by size: 5281 939 552 NdeI CA'TA_TG Cuts at: 387 3269 387 Size: 2882 3890 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6772 NotI GC'GGCC_GC Cuts at: 4219 4219 Size: 6772 NsiI A_TGCA'T Cuts at: 2893 2893 Size: 6772 NspI r_CATG'y Cuts at: 1132 1229 2521 4130 5675 1132 Size: 97 1292 1609 1545 2229 Fragments arranged by size: 2229 1609 1545 1292 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6772 PciI A'CATG_T Cuts at: 2517 5671 2517 Size: 3154 3618 PflMI CCAn_nnn'nTGG Cuts at: 1108 3502 1108 Size: 2394 4378 PmeI GTTT'AAAC Cuts at: 4076 4076 Size: 6772 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4841 4873 6354 6386 4841 Size: 32 1481 32 5227 Fragments arranged by size: 5227 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3229 3442 3229 Size: 213 6559 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6772 PsiI TTA'TAA Cuts at: 4342 4342 Size: 6772 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2007 2039 2397 2429 2007 Size: 32 358 32 6350 Fragments arranged by size: 6350 358 32 32 PstI C_TGCA'G Cuts at: 830 2108 4124 830 Size: 1278 2016 3478 Fragments arranged by size: 3478 2016 1278 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6772 PvuII CAG'CTG Cuts at: 2103 2255 2103 Size: 152 6620 SacI G_AGCT'C Cuts at: 2493 2493 Size: 6772 SalI G'TCGA_C Cuts at: 1936 4114 1936 Size: 2178 4594 SbfI CC_TGCA'GG Cuts at: 4124 4124 Size: 6772 ScaI AGT'ACT Cuts at: 2435 5020 6732 2435 Size: 2585 1712 2475 Fragments arranged by size: 2585 2475 1712 SfcI C'TryA_G Cuts at: 826 1045 2104 4120 5255 5936 6127 826 Size: 219 1059 2016 1135 681 191 1471 Fragments arranged by size: 2016 1471 1135 1059 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6772 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6772 SmaI CCC'GGG Cuts at: 1872 4099 1872 Size: 2227 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4876 5777 6039 820 Size: 197 926 2933 901 262 1553 Fragments arranged by size: 2933 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4563 493 Size: 4070 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6772 SphI G_CATG'C Cuts at: 1132 4130 1132 Size: 2998 3774 SspI AAT'ATT Cuts at: 5 52 2384 4696 5 Size: 47 2332 2312 2081 Fragments arranged by size: 2332 2312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6772 StyI C'CwwG_G Cuts at: 513 1065 2004 4211 513 Size: 552 939 2207 3074 Fragments arranged by size: 3074 2207 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3639 3956 4799 4816 4969 5154 5599 Size: 1255 2059 317 843 17 153 185 445 Cuts at: 5599 325 Size: 1498 Fragments arranged by size: 2059 1498 1255 843 445 317 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 2838 3307 Size: 274 80 33 51 1443 456 405 469 Cuts at: 3307 5018 6730 96 Size: 1711 1712 138 Fragments arranged by size: 1712 1711 1443 469 456 405 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2535 2567 2573 2605 6732 6764 2535 Size: 32 6 32 4127 32 2543 Fragments arranged by size: 4127 2543 32 32 32 6 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6772 XbaI T'CTAG_A Cuts at: 3289 4108 3289 Size: 819 5953 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6772 XmaI C'CCGG_G Cuts at: 1870 4097 1870 Size: 2227 4545 XmnI GAAnn'nnTTC Cuts at: 2746 3528 4901 2746 Size: 782 1373 4617 Fragments arranged by size: 4617 1373 782 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4580 276 Size: 53 83 186 936 3046 2468 Fragments arranged by size: 3046 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI