(Circular) (Six-base) MAPSORT of: pFN21AB9959.seq Check: 4379 from: 1 to: 6319 pFN21AB9959 6319 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2178 2239 2584 2723 3466 2178 Size: 61 345 139 743 5031 Fragments arranged by size: 5031 743 345 139 61 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2859 4129 278 Size: 53 83 186 936 1323 1270 2468 Fragments arranged by size: 2468 1323 1270 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3662 1937 Size: 1725 4594 Acc65I G'GTAC_C Cuts at: 2820 3640 2820 Size: 820 5499 AclI AA'CG_TT Cuts at: 1563 4446 4819 1563 Size: 2883 373 3063 Fragments arranged by size: 3063 2883 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2808 4382 5766 6034 1632 Size: 1176 1574 1384 268 1917 Fragments arranged by size: 1917 1574 1384 1176 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6122 AflIII A'CryG_T Cuts at: 1203 5218 6195 1203 Size: 4015 977 1327 Fragments arranged by size: 4015 1327 977 AgeI A'CCGG_T Cuts at: 5150 5150 Size: 6319 AhdI GACnn_n'nnGTC Cuts at: 5048 5048 Size: 6319 AleI CACnn'nnGTG Cuts at: 1166 2678 1166 Size: 1512 4807 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6319 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2954 2986 2954 Size: 32 6287 AlwNI CAG_nnn'CTG Cuts at: 3175 4062 5634 3175 Size: 887 1572 3860 Fragments arranged by size: 3860 1572 887 ApaLI G'TGCA_C Cuts at: 4376 5532 4376 Size: 1156 5163 ApoI r'AATT_y Cuts at: 1493 3628 3854 4006 6115 1493 Size: 2135 226 152 2109 1697 Fragments arranged by size: 2135 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4873 160 Size: 872 3841 1606 Fragments arranged by size: 3841 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 AvaI C'yCGr_G Cuts at: 1870 1943 2010 2123 2141 2686 3603 3644 1870 Size: 73 67 113 18 545 917 41 4545 Fragments arranged by size: 4545 917 545 113 73 67 41 18 BamHI G'GATC_C Cuts at: 3649 4016 3649 Size: 367 5952 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2147 2680 2820 2906 3332 Size: 325 217 627 360 533 140 86 426 Cuts at: 3332 3442 3640 5095 618 Size: 110 198 1455 1842 Fragments arranged by size: 1842 1455 627 533 426 360 325 217 198 140 110 86 BanII G_rGCy'C Cuts at: 1391 2098 2364 2394 2877 2939 3638 1391 Size: 707 266 30 483 62 699 4072 Fragments arranged by size: 4072 707 699 483 266 62 30 BbeI G_GCGC'C Cuts at: 3336 3336 Size: 6319 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3204 3474 928 Size: 971 1305 270 3773 Fragments arranged by size: 3773 1305 971 270 BbvCI CC'TCA_GC Cuts at: 2502 2502 Size: 6319 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2035 2069 4510 4544 1903 Size: 34 98 34 2441 34 3678 Fragments arranged by size: 3678 2441 98 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4211 5421 4211 Size: 1210 5109 BclI T'GATC_A Cuts at: 1548 1548 Size: 6319 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2378 3451 4930 Size: 107 122 71 631 605 706 1073 1479 Cuts at: 4930 136 Size: 1525 Fragments arranged by size: 1525 1479 1073 706 631 605 122 107 71 BglII A'GATC_T Cuts at: 6314 6314 Size: 6319 BlpI GC'TnA_GC Cuts at: 3024 3736 3024 Size: 712 5607 Bme1580I G_kGCm'C Cuts at: 1790 2150 2685 4380 5536 1790 Size: 360 535 1695 1156 2573 Fragments arranged by size: 2573 1695 1156 535 360 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6319 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2349 2617 4050 Size: 416 53 83 399 24 932 268 1433 Cuts at: 4050 5008 442 Size: 958 1753 Fragments arranged by size: 1753 1433 958 932 416 399 268 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6319 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2156 3283 2156 Size: 1127 5192 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2348 2911 3224 3269 3432 4979 1239 Size: 1109 563 313 45 163 1547 2579 Fragments arranged by size: 2579 1547 1109 563 313 163 45 Bpu10I CC'TnA_GC Cuts at: 2502 2846 2502 Size: 344 5975 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3293 4444 5309 5607 3293 Size: 1151 865 298 4005 Fragments arranged by size: 4005 1151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3041 4982 882 Size: 617 1542 1941 2219 Fragments arranged by size: 2219 1941 1542 617 BsaAI yAC'GTr Cuts at: 493 1204 4110 493 Size: 711 2906 2702 Fragments arranged by size: 2906 2702 711 BsaBI GATnn'nnATC Cuts at: 6313 6313 Size: 6319 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2048 2370 2856 3333 Size: 53 83 186 936 515 322 486 477 Cuts at: 3333 4126 4508 5143 275 Size: 793 382 635 1451 Fragments arranged by size: 1451 936 793 635 515 486 477 382 322 186 83 53 BsaWI w'CCGG_w Cuts at: 2090 4752 5150 5424 5571 5982 2090 Size: 2662 398 274 147 411 2427 Fragments arranged by size: 2662 2427 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2147 2177 3500 3530 1178 Size: 30 165 30 744 30 1323 30 3967 Fragments arranged by size: 3967 1323 744 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3203 3509 3518 3521 1186 Size: 2017 306 9 3 3984 Fragments arranged by size: 3984 2017 306 9 3 BseYI C'CCAG_C Cuts at: 2276 2285 2940 3270 5522 6018 2276 Size: 9 655 330 2252 496 2577 Fragments arranged by size: 2577 2252 655 496 330 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2974 1163 Size: 573 1238 4508 Fragments arranged by size: 4508 1238 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2830 3769 4530 5558 1540 Size: 275 186 829 939 761 1028 2301 Fragments arranged by size: 2301 1028 939 829 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2339 2364 2897 3300 4380 4465 5536 2339 Size: 25 533 403 1080 85 1071 3122 Fragments arranged by size: 3122 1080 1071 533 403 85 25 BsmI GAATG_Cn' Cuts at: 3830 3923 3830 Size: 93 6226 BsmBI CGTCTCn'nnnn_ Cuts at: 2326 2326 Size: 6319 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2150 2339 2364 2394 2685 2877 Size: 399 308 52 189 25 30 291 192 Cuts at: 2877 2897 2939 3300 3638 4380 4465 5536 1391 Size: 20 42 361 338 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 361 338 308 291 192 189 85 52 42 30 25 20 BspEI T'CCGG_A Cuts at: 5982 5982 Size: 6319 BspHI T'CATG_A Cuts at: 4208 5938 4208 Size: 1730 4589 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2178 2239 2584 2723 3019 3466 3674 844 Size: 1334 61 345 139 296 447 208 3489 Fragments arranged by size: 3489 1334 447 345 296 208 139 61 BsrBI CCG'CTC Cuts at: 1394 1823 2308 4206 1394 Size: 429 485 1898 3507 Fragments arranged by size: 3507 1898 485 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3111 4814 4988 65 Size: 1183 1863 1703 174 1396 Fragments arranged by size: 1863 1703 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3150 4963 5150 1700 Size: 110 1340 1813 187 2869 Fragments arranged by size: 2869 1813 1340 187 110 BsrGI T'GTAC_A Cuts at: 96 2193 96 Size: 2097 4222 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6319 BssSI C'ACGA_G Cuts at: 4379 5391 4379 Size: 1012 5307 BstAPI GCAn_nnn'nTGC Cuts at: 794 3175 3355 794 Size: 2381 180 3758 Fragments arranged by size: 3758 2381 180 BstBI TT'CG_AA Cuts at: 5123 5123 Size: 6319 BstEII G'GTnAC_C Cuts at: 1184 2206 2710 1184 Size: 1022 504 4793 Fragments arranged by size: 4793 1022 504 BstXI CCAn_nnnn'nTGG Cuts at: 3007 3424 3616 3007 Size: 417 192 5710 Fragments arranged by size: 5710 417 192 BstYI r'GATC_y Cuts at: 1972 2583 3481 3649 4016 4401 4418 5870 5956 Size: 611 898 168 367 385 17 1452 86 Cuts at: 5956 5968 6314 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 898 611 385 367 346 168 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2902 2902 Size: 6319 BtgI C'CryG_G Cuts at: 513 1065 2004 2151 2386 2877 3006 3132 513 Size: 552 939 147 235 491 129 126 3700 Fragments arranged by size: 3700 939 552 491 235 147 129 126 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3085 487 Size: 683 56 1859 3721 Fragments arranged by size: 3721 1859 683 56 BtsI GCAGTG_nn' Cuts at: 2931 2964 3835 4629 4649 2931 Size: 33 871 794 20 4601 Fragments arranged by size: 4601 871 794 33 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6319 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6284 DraI TTT'AAA Cuts at: 3623 3623 Size: 6319 DraIII CAC_nnn'GTG Cuts at: 2959 2959 Size: 6319 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2629 5326 809 Size: 507 1313 2697 1802 Fragments arranged by size: 2697 1802 1313 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2060 2250 2643 Size: 54 1425 50 275 18 230 190 393 Cuts at: 2643 2827 3766 4655 6284 8 Size: 184 939 889 1629 43 Fragments arranged by size: 1629 1425 939 889 393 275 230 190 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2827 3766 1537 Size: 275 1015 939 4090 Fragments arranged by size: 4090 1015 939 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3281 3507 4249 1353 Size: 1928 226 742 3423 Fragments arranged by size: 3423 1928 742 226 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4906 5280 5426 387 Size: 1064 138 3317 374 146 1280 Fragments arranged by size: 3317 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 2362 Size: 6319 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2348 2808 2911 3224 3269 3432 4382 Size: 393 716 460 103 313 45 163 950 Cuts at: 4382 4979 5766 6034 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 950 787 716 597 460 393 313 268 163 103 45 EcoO109I rG'GnC_Cy Cuts at: 2273 2530 2637 3209 3288 3398 3429 2273 Size: 257 107 572 79 110 31 5163 Fragments arranged by size: 5163 572 257 110 107 79 31 EcoRI G'AATT_C Cuts at: 3628 4006 3628 Size: 378 5941 FspI TGC'GCA Cuts at: 4825 4825 Size: 6319 HaeII r_GCGC'y Cuts at: 1678 2221 3336 5466 1678 Size: 543 1115 2130 2531 Fragments arranged by size: 2531 2130 1115 543 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2181 2213 2297 2329 3536 Size: 32 135 32 609 32 84 32 1207 Cuts at: 3536 3568 4960 4992 5034 5066 1373 Size: 32 1392 32 42 32 2626 Fragments arranged by size: 2626 1392 1207 609 135 84 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3663 3909 669 Size: 988 281 1725 246 3079 Fragments arranged by size: 3079 1725 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6319 HpaI GTT'AAC Cuts at: 3909 3909 Size: 6319 KasI G'GCGC_C Cuts at: 3332 3332 Size: 6319 KpnI G_GTAC'C Cuts at: 2824 3644 2824 Size: 820 5499 MfeI C'AATT_G Cuts at: 3918 3918 Size: 6319 MluI A'CGCG_T Cuts at: 6195 6195 Size: 6319 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5433 5617 1305 Size: 4128 184 2007 Fragments arranged by size: 4128 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4497 Fragments arranged by size: 4497 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2244 2678 3440 3614 4277 4636 4795 Size: 648 1078 434 762 174 663 359 159 Cuts at: 4795 518 Size: 2042 Fragments arranged by size: 2042 1078 762 663 648 434 359 174 159 MspA1I CmG'CkG Cuts at: 1606 2018 2053 2065 2944 3160 3735 4412 5560 Size: 412 35 12 879 216 575 677 1148 Cuts at: 5560 5805 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 879 677 575 412 245 216 35 12 NaeI GCC'GGC Cuts at: 3152 3152 Size: 6319 NarI GG'CG_CC Cuts at: 3333 3333 Size: 6319 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4828 Fragments arranged by size: 4828 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6319 NgoMIV G'CCGG_C Cuts at: 3150 3150 Size: 6319 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6319 NotI GC'GGCC_GC Cuts at: 3766 3766 Size: 6319 NspI r_CATG'y Cuts at: 1132 1229 3677 5222 1132 Size: 97 2448 1545 2229 Fragments arranged by size: 2448 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2932 1348 Size: 1584 4735 PciI A'CATG_T Cuts at: 5218 5218 Size: 6319 PflMI CCAn_nnn'nTGG Cuts at: 1108 2248 2959 1108 Size: 1140 711 4468 Fragments arranged by size: 4468 1140 711 PfoI T'CCnGG_A Cuts at: 2082 2185 2364 2082 Size: 103 179 6037 Fragments arranged by size: 6037 179 103 PmeI GTTT'AAAC Cuts at: 3623 3623 Size: 6319 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4388 4420 5901 5933 4388 Size: 32 1481 32 4774 Fragments arranged by size: 4774 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2273 2530 2637 3209 3398 3429 2273 Size: 257 107 572 189 31 5163 Fragments arranged by size: 5163 572 257 189 107 31 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6319 PsiI TTA'TAA Cuts at: 3889 3889 Size: 6319 PstI C_TGCA'G Cuts at: 830 3180 3671 830 Size: 2350 491 3478 Fragments arranged by size: 3478 2350 491 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6319 PvuII CAG'CTG Cuts at: 2944 2944 Size: 6319 SacI G_AGCT'C Cuts at: 2364 2364 Size: 6319 SalI G'TCGA_C Cuts at: 1936 3661 1936 Size: 1725 4594 SanDI GG'GwC_CC Cuts at: 2273 2637 3429 2273 Size: 364 792 5163 Fragments arranged by size: 5163 792 364 SapI GCTCTTCn'nnn_ Cuts at: 3281 3281 Size: 6319 SbfI CC_TGCA'GG Cuts at: 3671 3671 Size: 6319 ScaI AGT'ACT Cuts at: 4567 6279 4567 Size: 1712 4607 SfcI C'TryA_G Cuts at: 826 1045 3176 3556 3667 4802 5483 5674 826 Size: 219 2131 380 111 1135 681 191 1471 Fragments arranged by size: 2131 1471 1135 681 380 219 191 111 SfoI GGC'GCC Cuts at: 3334 3334 Size: 6319 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6319 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6319 SmaI CCC'GGG Cuts at: 1872 2012 3605 3646 1872 Size: 140 1593 41 4545 Fragments arranged by size: 4545 1593 140 41 SmlI C'TyrA_G Cuts at: 820 1017 1943 3308 4423 5324 5586 820 Size: 197 926 1365 1115 901 262 1553 Fragments arranged by size: 1553 1365 1115 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4110 493 Size: 3617 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6319 SphI G_CATG'C Cuts at: 1132 3677 1132 Size: 2545 3774 SrfI GCCC'GGGC Cuts at: 2012 2012 Size: 6319 SspI AAT'ATT Cuts at: 5 52 4243 5 Size: 47 4191 2081 Fragments arranged by size: 4191 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6319 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 2676 3381 3758 513 Size: 552 939 438 234 705 377 3074 Fragments arranged by size: 3074 939 705 552 438 377 234 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4346 4363 4516 4701 5146 325 Size: 1255 2766 17 153 185 445 1498 Fragments arranged by size: 2766 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2193 4565 6277 Size: 274 80 33 51 1443 216 2372 1712 Cuts at: 6277 96 Size: 138 Fragments arranged by size: 2372 1712 1443 274 216 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2147 2179 6279 6311 2147 Size: 32 4100 32 2155 Fragments arranged by size: 4100 2155 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6319 XbaI T'CTAG_A Cuts at: 3484 3655 3484 Size: 171 6148 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6319 XmaI C'CCGG_G Cuts at: 1870 2010 3603 3644 1870 Size: 140 1593 41 4545 Fragments arranged by size: 4545 1593 140 41 XmnI GAAnn'nnTTC Cuts at: 4448 4448 Size: 6319 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2857 4127 276 Size: 53 83 186 936 1323 1270 2468 Fragments arranged by size: 2468 1323 1270 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SwaI XcmI