(Circular) (Six-base) MAPSORT of: pFN21AB9940.seq Check: 9892 from: 1 to: 6676 pFN21AB9940 6676 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4486 278 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4019 1937 Size: 2082 4594 Acc65I G'GTAC_C Cuts at: 3162 3997 3162 Size: 835 5841 AclI AA'CG_TT Cuts at: 1563 4803 5176 1563 Size: 3240 373 3063 Fragments arranged by size: 3240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2825 2871 4739 6123 6391 1632 Size: 1193 46 1868 1384 268 1917 Fragments arranged by size: 1917 1868 1384 1193 268 46 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6479 AflIII A'CryG_T Cuts at: 1203 2941 3153 5575 6552 1203 Size: 1738 212 2422 977 1327 Fragments arranged by size: 2422 1738 1327 977 212 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 5405 5405 Size: 6676 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6676 AlwNI CAG_nnn'CTG Cuts at: 3233 4419 5991 3233 Size: 1186 1572 3918 Fragments arranged by size: 3918 1572 1186 ApaI G_GGCC'C Cuts at: 2869 2869 Size: 6676 ApaLI G'TGCA_C Cuts at: 2505 3426 4733 5889 2505 Size: 921 1307 1156 3292 Fragments arranged by size: 3292 1307 1156 921 ApoI r'AATT_y Cuts at: 1493 3132 3194 3268 3366 3848 3985 4211 4363 Size: 1639 62 74 98 482 137 226 152 Cuts at: 4363 6472 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1639 482 226 152 137 98 74 62 AseI AT'TA_AT Cuts at: 160 1032 5230 160 Size: 872 4198 1606 Fragments arranged by size: 4198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 4001 1870 Size: 73 2058 4545 Fragments arranged by size: 4545 2058 73 AvrII C'CTAG_G Cuts at: 3322 3322 Size: 6676 BamHI G'GATC_C Cuts at: 2290 4006 4373 2290 Size: 1716 367 4593 Fragments arranged by size: 4593 1716 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3162 3997 5452 618 Size: 325 217 627 1375 835 1455 1842 Fragments arranged by size: 1842 1455 1375 835 627 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2869 3839 3995 1391 Size: 654 824 970 156 4072 Fragments arranged by size: 4072 970 824 654 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2859 2977 3001 928 Size: 971 960 118 24 4603 Fragments arranged by size: 4603 971 960 118 24 BbvCI CC'TCA_GC Cuts at: 3544 3544 Size: 6676 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4867 4901 1903 Size: 34 2930 34 3678 Fragments arranged by size: 3678 2930 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2180 4568 5778 2180 Size: 2388 1210 3078 Fragments arranged by size: 3078 2388 1210 BclI T'GATC_A Cuts at: 1548 2577 3720 1548 Size: 1029 1143 4504 Fragments arranged by size: 4504 1143 1029 BfrBI ATG'CAT Cuts at: 3503 3503 Size: 6676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5287 136 Size: 107 122 71 631 605 3615 1525 Fragments arranged by size: 3615 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6671 6671 Size: 6676 BlpI GC'TnA_GC Cuts at: 4093 4093 Size: 6676 Bme1580I G_kGCm'C Cuts at: 1790 2509 2869 3430 4737 5893 1790 Size: 719 360 561 1307 1156 2573 Fragments arranged by size: 2573 1307 1156 719 561 360 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6676 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4407 5365 442 Size: 416 53 83 399 24 2990 958 1753 Fragments arranged by size: 2990 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 5336 1239 Size: 848 3249 2579 Fragments arranged by size: 3249 2579 848 Bpu10I CC'TnA_GC Cuts at: 2832 3544 2832 Size: 712 5964 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4801 5666 5964 4801 Size: 865 298 5513 Fragments arranged by size: 5513 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2814 5339 882 Size: 617 1315 2525 2219 Fragments arranged by size: 2525 2219 1315 617 BsaAI yAC'GTr Cuts at: 493 1204 2971 4467 493 Size: 711 1767 1496 2702 Fragments arranged by size: 2702 1767 1496 711 BsaBI GATnn'nnATC Cuts at: 3185 6670 3185 Size: 3485 3191 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3924 4483 4865 5500 Size: 53 83 186 936 2391 559 382 635 Cuts at: 5500 275 Size: 1451 Fragments arranged by size: 2391 1451 936 635 559 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2316 5109 5507 5781 5928 6339 2316 Size: 2793 398 274 147 411 2653 Fragments arranged by size: 2793 2653 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2443 2473 2812 2842 3222 Size: 30 165 30 1040 30 339 30 380 Cuts at: 3222 3252 1178 Size: 30 4602 Fragments arranged by size: 4602 1040 380 339 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2017 2861 1186 Size: 831 844 5001 Fragments arranged by size: 5001 844 831 BseYI C'CCAG_C Cuts at: 2265 3665 5879 6375 2265 Size: 1400 2214 496 2566 Fragments arranged by size: 2566 2214 1400 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2065 2184 3523 1163 Size: 573 329 119 1339 4316 Fragments arranged by size: 4316 1339 573 329 119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4126 4887 5915 1540 Size: 275 186 2125 761 1028 2301 Fragments arranged by size: 2301 2125 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2045 2509 2536 3430 4737 4822 5893 2045 Size: 464 27 894 1307 85 1071 2828 Fragments arranged by size: 2828 1307 1071 894 464 85 27 BsmI GAATG_Cn' Cuts at: 3505 3583 4187 4280 3505 Size: 78 604 93 5901 Fragments arranged by size: 5901 604 93 78 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2509 2536 2869 3430 3839 3995 Size: 399 255 464 27 333 561 409 156 Cuts at: 3995 4737 4822 5893 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 561 464 409 399 333 255 156 85 27 BspEI T'CCGG_A Cuts at: 6339 6339 Size: 6676 BspHI T'CATG_A Cuts at: 3063 4565 6295 3063 Size: 1502 1730 3444 Fragments arranged by size: 3444 1730 1502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4031 844 Size: 3187 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4563 1394 Size: 429 2740 3507 Fragments arranged by size: 3507 2740 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3754 3906 5171 5345 65 Size: 1183 2506 152 1265 174 1396 Fragments arranged by size: 2506 1396 1265 1183 174 152 BsrFI r'CCGG_y Cuts at: 1700 1810 3089 5320 5507 1700 Size: 110 1279 2231 187 2869 Fragments arranged by size: 2869 2231 1279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6676 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6676 BssSI C'ACGA_G Cuts at: 4736 5748 4736 Size: 1012 5664 BstAPI GCAn_nnn'nTGC Cuts at: 794 2162 3456 3554 3971 794 Size: 1368 1294 98 417 3499 Fragments arranged by size: 3499 1368 1294 417 98 BstBI TT'CG_AA Cuts at: 5480 5480 Size: 6676 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6676 BstXI CCAn_nnnn'nTGG Cuts at: 2975 2975 Size: 6676 BstYI r'GATC_y Cuts at: 1972 2290 2730 3341 4006 4373 4758 4775 6227 Size: 318 440 611 665 367 385 17 1452 Cuts at: 6227 6313 6325 6671 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 665 611 440 385 367 346 318 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2093 513 Size: 552 939 89 5096 Fragments arranged by size: 5096 939 552 89 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2510 2941 487 Size: 683 56 1284 431 4222 Fragments arranged by size: 4222 1284 683 431 56 BtsI GCAGTG_nn' Cuts at: 2999 4192 4986 5006 2999 Size: 1193 794 20 4669 Fragments arranged by size: 4669 1193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6676 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6641 DraI TTT'AAA Cuts at: 3492 3980 3492 Size: 488 6188 DraIII CAC_nnn'GTG Cuts at: 2342 3434 2342 Size: 1092 5584 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 5683 809 Size: 507 1284 3083 1802 Fragments arranged by size: 3083 1802 1284 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4123 5012 6641 Size: 54 1425 50 275 18 2293 889 1629 Cuts at: 6641 8 Size: 43 Fragments arranged by size: 2293 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4123 1537 Size: 275 2311 4090 Fragments arranged by size: 4090 2311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2111 2120 4606 1353 Size: 758 9 2486 3423 Fragments arranged by size: 3423 2486 758 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2011 2020 5263 5637 5783 387 Size: 1064 138 422 9 3243 374 146 1280 Fragments arranged by size: 3243 1280 1064 422 374 146 138 9 EcoICRI GAG'CTC Cuts at: 2043 2043 Size: 6676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2825 2871 4739 5336 6123 6391 Size: 393 455 738 46 1868 597 787 268 Cuts at: 6391 1239 Size: 1524 Fragments arranged by size: 1868 1524 787 738 597 455 393 268 46 EcoNI CCTnn'n_nnAGG Cuts at: 3632 3632 Size: 6676 EcoO109I rG'GnC_Cy Cuts at: 2572 2866 2572 Size: 294 6382 EcoRI G'AATT_C Cuts at: 3848 3985 4363 3848 Size: 137 378 6161 Fragments arranged by size: 6161 378 137 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2876 3574 2876 Size: 698 5978 FspI TGC'GCA Cuts at: 2936 5182 2936 Size: 2246 4430 HaeII r_GCGC'y Cuts at: 1678 5823 1678 Size: 4145 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5317 5349 5391 5423 1373 Size: 32 135 32 3745 32 42 32 2626 Fragments arranged by size: 3745 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4020 4266 669 Size: 988 281 2082 246 3079 Fragments arranged by size: 3079 2082 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6676 HpaI GTT'AAC Cuts at: 4266 4266 Size: 6676 KpnI G_GTAC'C Cuts at: 3166 4001 3166 Size: 835 5841 MfeI C'AATT_G Cuts at: 4275 4275 Size: 6676 MluI A'CGCG_T Cuts at: 6552 6552 Size: 6676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2211 3037 5790 5974 1305 Size: 906 826 2753 184 2007 Fragments arranged by size: 2753 2007 906 826 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4854 Fragments arranged by size: 4854 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2529 3569 4634 4993 5152 518 Size: 648 960 403 1040 1065 359 159 2042 Fragments arranged by size: 2042 1065 1040 960 648 403 359 159 MspA1I CmG'CkG Cuts at: 1606 2065 2269 4092 4769 5917 6162 1606 Size: 459 204 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 459 245 204 NcoI C'CATG_G Cuts at: 513 1065 2004 2093 513 Size: 552 939 89 5096 Fragments arranged by size: 5096 939 552 89 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6676 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NsiI A_TGCA'T Cuts at: 3505 3505 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 3157 3210 4034 5579 1132 Size: 97 1928 53 824 1545 2229 Fragments arranged by size: 2229 1928 1545 824 97 53 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6676 PciI A'CATG_T Cuts at: 3153 5575 3153 Size: 2422 4254 PflMI CCAn_nnn'nTGG Cuts at: 1108 2249 3172 1108 Size: 1141 923 4612 Fragments arranged by size: 4612 1141 923 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4745 4777 6258 6290 4745 Size: 32 1481 32 5131 Fragments arranged by size: 5131 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2179 1613 Size: 566 6110 PsiI TTA'TAA Cuts at: 4246 4246 Size: 6676 PspOMI G'GGCC_C Cuts at: 2865 2865 Size: 6676 PstI C_TGCA'G Cuts at: 830 2018 3150 3653 4028 830 Size: 1188 1132 503 375 3478 Fragments arranged by size: 3478 1188 1132 503 375 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 SacI G_AGCT'C Cuts at: 2045 2045 Size: 6676 SalI G'TCGA_C Cuts at: 1936 4018 1936 Size: 2082 4594 SapI GCTCTTCn'nnn_ Cuts at: 2111 2111 Size: 6676 SbfI CC_TGCA'GG Cuts at: 4028 4028 Size: 6676 ScaI AGT'ACT Cuts at: 2721 4924 6636 2721 Size: 2203 1712 2761 Fragments arranged by size: 2761 2203 1712 SfcI C'TryA_G Cuts at: 826 1045 2014 2331 2892 3146 3649 4024 5159 Size: 219 969 317 561 254 503 375 1135 Cuts at: 5159 5840 6031 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 969 681 561 503 375 317 254 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6676 SmaI CCC'GGG Cuts at: 1872 4003 1872 Size: 2131 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4780 5681 5943 820 Size: 197 926 2837 901 262 1553 Fragments arranged by size: 2837 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4467 493 Size: 3974 2702 SpeI A'CTAG_T Cuts at: 152 2176 2671 152 Size: 2024 495 4157 Fragments arranged by size: 4157 2024 495 SphI G_CATG'C Cuts at: 1132 4034 1132 Size: 2902 3774 SspI AAT'ATT Cuts at: 5 52 4600 5 Size: 47 4548 2081 Fragments arranged by size: 4548 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6676 StyI C'CwwG_G Cuts at: 513 1065 2004 2093 2357 3322 3628 4115 513 Size: 552 939 89 264 965 306 487 3074 Fragments arranged by size: 3074 965 939 552 487 306 264 89 SwaI ATTT'AAAT Cuts at: 3492 3492 Size: 6676 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2279 3040 3697 4703 4720 4873 5058 Size: 1255 699 761 657 1006 17 153 185 Cuts at: 5058 5503 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1006 761 699 657 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2719 2776 3054 Size: 274 80 33 51 1443 742 57 278 Cuts at: 3054 3150 4922 6634 96 Size: 96 1772 1712 138 Fragments arranged by size: 1772 1712 1443 742 278 274 138 96 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2443 2475 6636 6668 2443 Size: 32 4161 32 2451 Fragments arranged by size: 4161 2451 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6676 XbaI T'CTAG_A Cuts at: 4012 4012 Size: 6676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2689 2689 Size: 6676 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6676 XmaI C'CCGG_G Cuts at: 1870 4001 1870 Size: 2131 4545 XmnI GAAnn'nnTTC Cuts at: 3198 4805 3198 Size: 1607 5069 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4484 276 Size: 53 83 186 936 2950 2468 Fragments arranged by size: 2950 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI