Restriction map for
pFN21AB9937
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AccI AccIII AclI AflII AflIII
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI AspEI AspI
AviII AvrII BcgI BciVI BfrI
BglI BglII BlnI BsaBI BsaWI
BseAI BsgI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstXI
BstZ17I ClaI CpoI DraI DraIII
Eam1105I EcoO65I EcoRI EcoRV FseI
FspI HpaI MamI MfeI MluI
MroI MseI MunI NaeI NcoI
NgoMIV NotI NruI PacI PflFI
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfiI SmaI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 778 GACGTC GACGT^C
AlwNI 1513 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 609 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 1208 TGGCCA TGG^CCA
BamHI 227 GGATCC G^GATCC
BbeI 145 GGCGCC GGCGC^C
BbsI 1242 GAAGAC GAAGAC(2/6)
BbvCI 1111 GCTGAGG CCTCAGC(-5/-2)
BclI 1478 TGATCA T^GATCA
BlpI 1177 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1177 GCTGAGC GC^T[ACGT]AGC
BpuAI 1242 GAAGAC GAAGAC(2/6)
BsaI 847 GGTCTC GGTCTC(1/5)
BsiEI 83 CGGCCG CG[GA][CT]^CG
BsmI 477 GAATGC GAATGC(1/-1)
BsrFI 142 ACCGGC [GA]^CCGG[CT]
BstBI 372 TTCGAA TT^CGAA
CelII 1177 GCTGAGC GC^T[ACGT]AGC
Cfr10I 142 ACCGGC [GA]^CCGG[CT]
DraII 770 AGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 772 GACCCTGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 83 CGGCCG C^GGCCG
EclXI 83 CGGCCG C^GGCCG
Eco52I 83 CGGCCG C^GGCCG
EcoNI 1542 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 770 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoT22I 479 ATGCAT ATGCA^T
FauI 1290 GCGGG CCCGC(4/6)
FbaI 1478 TGATCA T^GATCA
HgaI 4 GCGTC GACGC(5/10)
HincII 1000 GTCAAC GT[CT]^[GA]AC
HindII 1000 GTCAAC GT[CT]^[GA]AC
KasI 145 GGCGCC G^GCGCC
KspI 149 CCGCGG CCGC^GG
MluNI 1208 TGGCCA TGG^CCA
MscI 1208 TGGCCA TGG^CCA
NarI 145 GGCGCC GG^CGCC
NdeI 482 CATATG CA^TATG
NsiI 479 ATGCAT ATGCA^T
NspI 978 GCATGC [GA]CATG^[CT]
NspV 372 TTCGAA TT^CGAA
PaeR7I 1360 CTCGAG C^TCGAG
PpuMI 770 AGGACCC [GA]G^G[AT]CC[CT]
SacII 149 CCGCGG CCGC^GG
ScaI 1526 AGTACT AGT^ACT
SfoI 145 GGCGCC GGC^GCC
SfuI 372 TTCGAA TT^CGAA
SgrAI 141 CACCGGCG C[GA]^CCGG[CT]G
SphI 978 GCATGC GCATG^C
XcmI 828 CCAGACATTGAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1360 CTCGAG C^TCGAG
XmnI 609 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Acc65I 731 GGTACC G^GTACC
823 GGTACC
AccII 150 CGCG CG^CG
1219 CGCG
AcsI 366 AAATTC [GA]^AATT[CT]
697 GAATTT
AocI 352 CCTCAGG CC^T[ACGT]AGG
1546 CCTGAGG
ApoI 366 AAATTC [GA]^AATT[CT]
697 GAATTT
Asp718I 731 GGTACC G^GTACC
823 GGTACC
AspHI 1065 GAGCTC G[AT]GC[AT]^C
1180 GAGCTC
AvaII 771 GGACC G^G[AT]CC
1419 GGTCC
BanII 1065 GAGCTC G[GA]GC[CT]^C
1180 GAGCTC
BbrPI 812 CACGTG CAC^GTG
1474 CACGTG
BpmI 937 CTCCAG CTGGAG(16/14)
61 CTGGAG
BsaAI 812 CACGTG [CT]AC^GT[GA]
1474 CACGTG
BsiHKAI 1065 GAGCTC G[AT]GC[AT]^C
1180 GAGCTC
BsrI 1529 ACTGG ACTGG(1/-1)
1035 CCAGT
BstUI 150 CGCG CG^CG
1219 CGCG
Bsu36I 352 CCTCAGG CC^T[ACGT]AGG
1546 CCTGAGG
DsaI 149 CCGCGG C^C[GA][CT]GG
818 CCGTGG
EaeI 83 CGGCCG [CT]^GGCC[GA]
1208 TGGCCA
Eco81I 352 CCTCAGG CC^T[ACGT]AGG
1546 CCTGAGG
HindIII 513 AAGCTT A^AGCTT
586 AAGCTT
HphI 1377 GGTGA GGTGA(8/7)
140 TCACC
KpnI 731 GGTACC GGTAC^C
823 GGTACC
MslI 648 CATAGGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
897 CATCAAGATG
MvnI 150 CGCG CG^CG
1219 CGCG
NheI 324 GCTAGC G^CTAGC
1017 GCTAGC
PmaCI 812 CACGTG CAC^GTG
1474 CACGTG
PmlI 812 CACGTG CAC^GTG
1474 CACGTG
PvuII 1105 CAGCTG CAG^CTG
1516 CAGCTG
SacI 1065 GAGCTC GAGCT^C
1180 GAGCTC
SapI 639 GAAGAGC GCTCTTC(1/4)
802 GAAGAGC
TfiI 682 GATTC G^A[AT]TC
910 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
Aor51HI AvaI BanI BbvI BcnI
BfaI BmyI BsaHI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EarI Eco47III EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII