(Circular) (Six-base) MAPSORT of: pFN21AB9937.seq Check: 1069 from: 1 to: 6271 pFN21AB9937 6271 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2787 4081 278 Size: 53 83 186 936 1251 1294 2468 Fragments arranged by size: 2468 1294 1251 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 2736 2828 3592 2736 Size: 92 764 5415 Fragments arranged by size: 5415 764 92 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2726 4334 5718 5986 1632 Size: 1094 1608 1384 268 1917 Fragments arranged by size: 1917 1608 1384 1094 268 AfeI AGC'GCT Cuts at: 2032 2969 3027 3260 2032 Size: 937 58 233 5043 Fragments arranged by size: 5043 937 233 58 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 5170 6147 1203 Size: 3967 977 1327 Fragments arranged by size: 3967 1327 977 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 3523 4014 5586 3523 Size: 491 1572 4208 Fragments arranged by size: 4208 1572 491 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 2371 2702 3580 3806 3958 6067 1493 Size: 878 331 878 226 152 2109 1697 Fragments arranged by size: 2109 1697 878 878 331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 2105 3365 3378 3596 1870 Size: 73 162 1260 13 218 4545 Fragments arranged by size: 4545 1260 218 162 73 13 BamHI G'GATC_C Cuts at: 2232 3601 3968 2232 Size: 1369 367 4535 Fragments arranged by size: 4535 1369 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2150 2736 2828 3592 5047 Size: 325 217 627 363 586 92 764 1455 Cuts at: 5047 618 Size: 1842 Fragments arranged by size: 1842 1455 764 627 586 363 325 217 92 BanII G_rGCy'C Cuts at: 1391 3074 3189 3590 1391 Size: 1683 115 401 4072 Fragments arranged by size: 4072 1683 401 115 BbeI G_GCGC'C Cuts at: 2154 2154 Size: 6271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3254 928 Size: 971 1355 3945 Fragments arranged by size: 3945 1355 971 BbvCI CC'TCA_GC Cuts at: 3117 3117 Size: 6271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4462 4496 1903 Size: 34 2525 34 3678 Fragments arranged by size: 3678 2525 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4163 5373 4163 Size: 1210 5061 BclI T'GATC_A Cuts at: 1548 3483 1548 Size: 1935 4336 BfrBI ATG'CAT Cuts at: 2486 2486 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4882 136 Size: 107 122 71 631 605 3210 1525 Fragments arranged by size: 3210 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 3183 3688 3183 Size: 505 5766 Bme1580I G_kGCm'C Cuts at: 1790 3103 4332 5488 1790 Size: 1313 1229 1156 2573 Fragments arranged by size: 2573 1313 1229 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3034 3544 4002 Size: 416 53 83 399 24 1617 510 458 Cuts at: 4002 4960 442 Size: 958 1753 Fragments arranged by size: 1753 1617 958 510 458 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2333 3026 1056 Size: 1277 693 4301 Fragments arranged by size: 4301 1277 693 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2064 2623 2064 Size: 559 5712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2087 2927 4931 1239 Size: 848 840 2004 2579 Fragments arranged by size: 2579 2004 848 840 Bpu10I CC'TnA_GC Cuts at: 3117 3117 Size: 6271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2843 3515 4396 5261 5559 2843 Size: 672 881 865 298 3555 Fragments arranged by size: 3555 881 865 672 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2858 4934 882 Size: 617 1359 2076 2219 Fragments arranged by size: 2219 2076 1359 617 BsaAI yAC'GTr Cuts at: 493 1204 2819 3481 4062 493 Size: 711 1615 662 581 2702 Fragments arranged by size: 2702 1615 711 662 581 BsaBI GATnn'nnATC Cuts at: 6265 6265 Size: 6271 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2151 2784 4078 Size: 53 83 186 936 476 142 633 1294 Cuts at: 4078 4460 5095 275 Size: 382 635 1451 Fragments arranged by size: 1451 1294 936 635 633 476 382 186 142 83 53 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2060 2090 2201 2231 2939 Size: 30 165 30 657 30 111 30 708 Cuts at: 2939 2969 1178 Size: 30 4480 Fragments arranged by size: 4480 708 657 165 111 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2346 2414 2846 2948 2975 1186 Size: 1160 68 432 102 27 4482 Fragments arranged by size: 4482 1160 432 102 68 27 BseYI C'CCAG_C Cuts at: 2111 2385 3108 5474 5970 2111 Size: 274 723 2366 496 2412 Fragments arranged by size: 2412 2366 723 496 274 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5698 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2091 3721 4482 5510 1540 Size: 275 186 90 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 90 BsiHKAI G_wGCw'C Cuts at: 3074 3189 4332 4417 5488 3074 Size: 115 1143 85 1071 3857 Fragments arranged by size: 3857 1143 1071 115 85 BsmI GAATG_Cn' Cuts at: 2488 3782 3875 2488 Size: 1294 93 4884 Fragments arranged by size: 4884 1294 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3074 3103 3189 3590 4332 4417 5488 Size: 399 1284 29 86 401 742 85 1071 Cuts at: 5488 1391 Size: 2174 Fragments arranged by size: 2174 1284 1071 742 401 399 86 85 29 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3626 844 Size: 2782 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4766 4940 65 Size: 1183 3518 174 1396 Fragments arranged by size: 3518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2147 4915 5102 1700 Size: 110 337 2768 187 2869 Fragments arranged by size: 2869 2768 337 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6271 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6271 BssSI C'ACGA_G Cuts at: 4331 5343 4331 Size: 1012 5259 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6271 BstBI TT'CG_AA Cuts at: 2378 5075 2378 Size: 2697 3574 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2232 2263 3360 3601 3968 4353 4370 5822 Size: 260 31 1097 241 367 385 17 1452 Cuts at: 5822 5908 5920 6266 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1097 385 367 346 260 241 86 31 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 3552 2358 Size: 1194 5077 BtgI C'CryG_G Cuts at: 513 1065 2004 2154 2823 513 Size: 552 939 150 669 3961 Fragments arranged by size: 3961 939 669 552 150 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3281 3787 4581 4601 3281 Size: 506 794 20 4951 Fragments arranged by size: 4951 794 506 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2783 5278 809 Size: 507 1467 2495 1802 Fragments arranged by size: 2495 1802 1467 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 3213 3718 Size: 54 1425 50 275 18 258 1125 505 Cuts at: 3718 4607 6236 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1125 889 505 275 258 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2088 3718 1537 Size: 275 276 1630 4090 Fragments arranged by size: 4090 1630 276 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2355 2639 2802 4201 1353 Size: 1002 284 163 1399 3423 Fragments arranged by size: 3423 1399 1002 284 163 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2040 4858 5232 5378 387 Size: 1064 138 451 2818 374 146 1280 Fragments arranged by size: 2818 1280 1064 451 374 146 138 EcoICRI GAG'CTC Cuts at: 3072 3187 3072 Size: 115 6156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2087 2726 2927 4334 4931 5718 5986 Size: 393 455 639 201 1407 597 787 268 Cuts at: 5986 1239 Size: 1524 Fragments arranged by size: 1524 1407 787 639 597 455 393 268 201 EcoNI CCTnn'n_nnAGG Cuts at: 3551 3551 Size: 6271 EcoO109I rG'GnC_Cy Cuts at: 2776 2776 Size: 6271 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3563 3563 Size: 6271 FspI TGC'GCA Cuts at: 4777 4777 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 2034 2154 2971 3029 3262 5418 1678 Size: 356 120 817 58 233 2156 2531 Fragments arranged by size: 2531 2156 817 356 233 120 58 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2060 2092 2740 2772 3359 Size: 32 135 32 488 32 648 32 587 Cuts at: 3359 3391 4912 4944 4986 5018 1373 Size: 32 1521 32 42 32 2626 Fragments arranged by size: 2626 1521 648 587 488 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3007 3615 3861 669 Size: 988 281 1069 608 246 3079 Fragments arranged by size: 3079 1069 988 608 281 246 HindIII A'AGCT_T Cuts at: 748 2518 2591 748 Size: 1770 73 4428 Fragments arranged by size: 4428 1770 73 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KasI G'GCGC_C Cuts at: 2150 2150 Size: 6271 KpnI G_GTAC'C Cuts at: 2740 2832 3596 2740 Size: 92 764 5415 Fragments arranged by size: 5415 764 92 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5385 5569 1305 Size: 4080 184 2007 Fragments arranged by size: 4080 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3215 10 Size: 54 1425 343 1383 3066 Fragments arranged by size: 3066 1425 1383 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2657 2906 4229 4588 4747 518 Size: 648 1491 249 1323 359 159 2042 Fragments arranged by size: 2042 1491 1323 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2156 3112 3523 3687 4364 5512 5757 1606 Size: 550 956 411 164 677 1148 245 2120 Fragments arranged by size: 2120 1148 956 677 550 411 245 164 NarI GG'CG_CC Cuts at: 2151 2151 Size: 6271 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4780 Fragments arranged by size: 4780 939 552 NdeI CA'TA_TG Cuts at: 387 2488 387 Size: 2101 4170 NheI G'CTAG_C Cuts at: 1052 2329 3022 1052 Size: 1277 693 4301 Fragments arranged by size: 4301 1277 693 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NsiI A_TGCA'T Cuts at: 2488 2488 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 2987 3629 5174 1132 Size: 97 1758 642 1545 2229 Fragments arranged by size: 2229 1758 1545 642 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6271 PfoI T'CCnGG_A Cuts at: 2943 2943 Size: 6271 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PmlI CAC'GTG Cuts at: 2819 3481 2819 Size: 662 5609 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2776 2776 Size: 6271 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PspXI vC'TCGA_Gb Cuts at: 3365 3365 Size: 6271 PstI C_TGCA'G Cuts at: 830 2701 3288 3345 3623 830 Size: 1871 587 57 278 3478 Fragments arranged by size: 3478 1871 587 278 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 3112 3523 3112 Size: 411 5860 SacI G_AGCT'C Cuts at: 3074 3189 3074 Size: 115 6156 SacII CC_GC'GG Cuts at: 2157 2157 Size: 6271 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SapI GCTCTTCn'nnn_ Cuts at: 2639 2802 2639 Size: 163 6108 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 3533 4519 6231 3533 Size: 986 1712 3573 Fragments arranged by size: 3573 1712 986 SfcI C'TryA_G Cuts at: 826 1045 2026 2697 3284 3341 3619 4754 5435 Size: 219 981 671 587 57 278 1135 681 Cuts at: 5435 5626 826 Size: 191 1471 Fragments arranged by size: 1471 1135 981 681 671 587 278 219 191 57 SfoI GGC'GCC Cuts at: 2152 2152 Size: 6271 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 2147 1700 Size: 447 5824 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2858 3365 3494 4375 5276 5538 Size: 197 926 915 507 129 881 901 262 Cuts at: 5538 820 Size: 1553 Fragments arranged by size: 1553 926 915 901 881 507 262 197 129 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 2987 3629 1132 Size: 1855 642 3774 Fragments arranged by size: 3774 1855 642 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 2004 2295 3351 3462 3710 513 Size: 552 939 291 1056 111 248 3074 Fragments arranged by size: 3074 1056 939 552 291 248 111 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4298 4315 4468 4653 5098 325 Size: 1255 2718 17 153 185 445 1498 Fragments arranged by size: 2718 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3531 4517 6229 Size: 274 80 33 51 1443 1554 986 1712 Cuts at: 6229 96 Size: 138 Fragments arranged by size: 1712 1554 1443 986 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2939 2971 6231 6263 2939 Size: 32 3260 32 2947 Fragments arranged by size: 3260 2947 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6271 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2840 2840 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 3365 1943 Size: 1422 4849 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 2618 4400 2618 Size: 1782 4489 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2785 4079 276 Size: 53 83 186 936 1251 1294 2468 Fragments arranged by size: 2468 1294 1251 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI PacI PspOMI PsrI RsrII SanDI SexAI SfiI SrfI SwaI