(Circular) (Six-base) MAPSORT of: pFN21AB9859.seq Check: 9858 from: 1 to: 5905 pFN21AB9859 5905 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 2477 3968 5352 5620 1632 Size: 508 337 1491 1384 268 1917 Fragments arranged by size: 1917 1491 1384 508 337 268 AfeI AGC'GCT Cuts at: 2165 2454 2165 Size: 289 5616 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2748 4804 5781 1203 Size: 1545 2056 977 1327 Fragments arranged by size: 2056 1545 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 4634 4634 Size: 5905 AleI CACnn'nnGTG Cuts at: 1166 2762 1166 Size: 1596 4309 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5905 AlwNI CAG_nnn'CTG Cuts at: 2905 3648 5220 2905 Size: 743 1572 3590 Fragments arranged by size: 3590 1572 743 ApaI G_GGCC'C Cuts at: 2143 2143 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 3214 3440 3592 5701 1493 Size: 1721 226 152 2109 1697 Fragments arranged by size: 2109 1721 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 2097 2615 2669 3230 1870 Size: 73 154 518 54 561 4545 Fragments arranged by size: 4545 561 518 154 73 54 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 2796 3171 3226 4681 Size: 325 217 627 277 732 375 55 1455 Cuts at: 4681 618 Size: 1842 Fragments arranged by size: 1842 1455 732 627 375 325 277 217 55 BanII G_rGCy'C Cuts at: 1391 2143 2204 3224 1391 Size: 752 61 1020 4072 Fragments arranged by size: 4072 1020 752 61 BbeI G_GCGC'C Cuts at: 2068 2068 Size: 5905 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2720 928 Size: 971 821 4113 Fragments arranged by size: 4113 971 821 BbvCI CC'TCA_GC Cuts at: 2970 2976 3012 3030 2970 Size: 6 36 18 5845 Fragments arranged by size: 5845 36 18 6 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2335 2369 2767 2801 4096 4130 1903 Size: 34 398 34 398 34 1295 34 3678 Fragments arranged by size: 3678 1295 398 398 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3063 3797 5007 3063 Size: 734 1210 3961 Fragments arranged by size: 3961 1210 734 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2331 2375 2870 Size: 107 122 71 631 605 659 44 495 Cuts at: 2870 3169 4516 136 Size: 299 1347 1525 Fragments arranged by size: 1525 1347 659 631 605 495 299 122 107 71 44 BglII A'GATC_T Cuts at: 5900 5900 Size: 5905 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2143 2154 2801 3174 3966 5122 1790 Size: 353 11 647 373 792 1156 2573 Fragments arranged by size: 2573 1156 792 647 373 353 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5905 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2640 3636 4594 Size: 416 53 83 399 24 1223 996 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1223 996 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2453 2492 2936 4565 1239 Size: 1214 39 444 1629 2579 Fragments arranged by size: 2579 1629 1214 444 39 Bpu10I CC'TnA_GC Cuts at: 2970 2976 3012 3030 2970 Size: 6 36 18 5845 Fragments arranged by size: 5845 36 18 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2672 4030 4895 5193 2672 Size: 1358 865 298 3384 Fragments arranged by size: 3384 1358 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4568 882 Size: 617 3069 2219 Fragments arranged by size: 3069 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2065 3712 4094 4729 Size: 53 83 186 936 532 1647 382 635 Cuts at: 4729 275 Size: 1451 Fragments arranged by size: 1647 1451 936 635 532 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2282 1186 Size: 1096 4809 BseYI C'CCAG_C Cuts at: 2041 2086 2328 2420 2964 2988 3006 3036 5108 Size: 45 242 92 544 24 18 30 2072 Cuts at: 5108 5604 2041 Size: 496 2342 Fragments arranged by size: 2342 2072 544 496 242 92 45 30 24 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2681 2864 1163 Size: 573 945 183 4204 Fragments arranged by size: 4204 945 573 183 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2317 2631 3355 4116 5144 1540 Size: 275 186 316 314 724 761 1028 2301 Fragments arranged by size: 2301 1028 761 724 316 314 275 186 BsiHKAI G_wGCw'C Cuts at: 2204 3966 4051 5122 2204 Size: 1762 85 1071 2987 Fragments arranged by size: 2987 1762 1071 85 BsmI GAATG_Cn' Cuts at: 3416 3509 3416 Size: 93 5812 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2143 2154 2204 2801 3174 3224 3966 Size: 399 353 11 50 597 373 50 742 Cuts at: 3966 4051 5122 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 597 399 373 353 85 50 50 11 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 3794 5524 3794 Size: 1730 4175 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2269 3792 1394 Size: 429 446 1523 3507 Fragments arranged by size: 3507 1523 446 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4400 4574 65 Size: 1183 3152 174 1396 Fragments arranged by size: 3152 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2626 2677 3174 4549 4736 1700 Size: 110 207 609 51 497 1375 187 2869 Fragments arranged by size: 2869 1375 609 497 207 187 110 51 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5905 BssHII G'CGCG_C Cuts at: 1925 2298 1925 Size: 373 5532 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 2842 794 Size: 2048 3857 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5905 BstXI CCAn_nnnn'nTGG Cuts at: 2648 2648 Size: 5905 BstYI r'GATC_y Cuts at: 1972 3235 3602 3987 4004 5456 5542 5554 5900 Size: 1263 367 385 17 1452 86 12 346 Cuts at: 5900 1972 Size: 1977 Fragments arranged by size: 1977 1452 1263 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2054 2317 2718 513 Size: 552 989 263 401 3700 Fragments arranged by size: 3700 989 552 401 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3421 4215 4235 3421 Size: 794 20 5091 Fragments arranged by size: 5091 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5905 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2233 4912 809 Size: 507 917 2679 1802 Fragments arranged by size: 2679 1802 917 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2144 2314 2628 Size: 54 1425 50 275 18 314 170 314 Cuts at: 2628 3352 4241 5870 8 Size: 724 889 1629 43 Fragments arranged by size: 1629 1425 889 724 314 314 275 170 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2314 2628 3352 1537 Size: 275 502 314 724 4090 Fragments arranged by size: 4090 724 502 314 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3835 1353 Size: 2482 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4492 4866 5012 387 Size: 1064 138 2903 374 146 1280 Fragments arranged by size: 2903 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2202 2202 Size: 5905 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2453 2477 2492 2936 3968 4565 Size: 393 508 313 24 15 444 1032 597 Cuts at: 4565 5352 5620 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1032 787 597 508 444 393 313 268 24 15 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 5905 EcoO109I rG'GnC_Cy Cuts at: 2139 2208 2248 2139 Size: 69 40 5796 Fragments arranged by size: 5796 69 40 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FseI GG_CCGG'CC Cuts at: 2630 2630 Size: 5905 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 2032 2068 2131 2167 2456 5052 1678 Size: 354 36 63 36 289 2596 2531 Fragments arranged by size: 2596 2531 354 289 63 36 36 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4546 4578 4620 4652 1373 Size: 32 135 32 2974 32 42 32 2626 Fragments arranged by size: 2974 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3249 3495 669 Size: 988 281 1311 246 3079 Fragments arranged by size: 3079 1311 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KasI G'GCGC_C Cuts at: 2064 2064 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2730 3219 5019 5203 1305 Size: 1425 489 1800 184 2007 Fragments arranged by size: 2007 1800 1425 489 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4083 Fragments arranged by size: 4083 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2762 3863 4222 4381 518 Size: 648 1596 1101 359 159 2042 Fragments arranged by size: 2042 1596 1101 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2086 2328 2563 2657 3321 3998 5146 Size: 450 30 242 235 94 664 677 1148 Cuts at: 5146 5391 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 664 450 245 242 235 94 30 NaeI GCC'GGC Cuts at: 2019 2628 2019 Size: 609 5296 NarI GG'CG_CC Cuts at: 2065 2065 Size: 5905 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NgoMIV G'CCGG_C Cuts at: 2017 2626 2017 Size: 609 5296 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2752 3263 4808 1132 Size: 97 1523 511 1545 2229 Fragments arranged by size: 2229 1545 1523 511 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5905 PciI A'CATG_T Cuts at: 2748 4804 2748 Size: 2056 3849 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5905 PfoI T'CCnGG_A Cuts at: 2204 2204 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2208 2248 2208 Size: 40 5865 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 2139 2139 Size: 5905 PspXI vC'TCGA_Gb Cuts at: 2669 2669 Size: 5905 PstI C_TGCA'G Cuts at: 830 2838 3257 830 Size: 2008 419 3478 Fragments arranged by size: 3478 2008 419 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 PvuII CAG'CTG Cuts at: 2563 2563 Size: 5905 SacI G_AGCT'C Cuts at: 2204 2204 Size: 5905 SacII CC_GC'GG Cuts at: 2057 2057 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SanDI GG'GwC_CC Cuts at: 2248 2248 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SexAI A'CCwGG_T Cuts at: 2904 2904 Size: 5905 SfcI C'TryA_G Cuts at: 826 1045 2834 3253 4388 5069 5260 826 Size: 219 1789 419 1135 681 191 1471 Fragments arranged by size: 1789 1471 1135 681 419 219 191 SfoI GGC'GCC Cuts at: 2066 2066 Size: 5905 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5905 SmaI CCC'GGG Cuts at: 1872 2617 3232 1872 Size: 745 615 4545 Fragments arranged by size: 4545 745 615 SmlI C'TyrA_G Cuts at: 820 1017 1943 2669 2687 4009 4910 5172 820 Size: 197 926 726 18 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 726 262 197 18 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SrfI GCCC'GGGC Cuts at: 2617 2617 Size: 5905 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 3344 513 Size: 552 2279 3074 Fragments arranged by size: 3074 2279 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 3111 3153 3159 3932 3949 4102 Size: 1255 498 1033 42 6 773 17 153 Cuts at: 4102 4287 4732 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1033 773 498 445 185 153 42 17 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2745 2751 2847 Size: 274 80 33 51 1443 768 6 96 Cuts at: 2847 4151 5863 96 Size: 1304 1712 138 Fragments arranged by size: 1712 1443 1304 768 274 138 96 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5865 5897 5865 Size: 32 5873 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 2669 1943 Size: 726 5179 XmaI C'CCGG_G Cuts at: 1870 2615 3230 1870 Size: 745 615 4545 Fragments arranged by size: 4545 745 615 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FspAI NruI NsiI PacI PmlI PsrI RsrII SapI SfiI SwaI XcmI