Restriction map for
pFN21AB9913
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
CelII CfoI ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MluNI MscI MspA1I MunI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AccI 802 GTCTAC GT^[AC][GT]AC
AccII 33 CGCG CG^CG
AccIII 12 TCCGGA T^CCGGA
Alw44I 774 GTGCAC G^TGCAC
AocI 263 CCTCAGG CC^T[ACGT]AGG
ApaLI 774 GTGCAC G^TGCAC
Asp700I 817 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 774 GTGCAC G[AT]GC[AT]^C
BbsI 340 GAAGAC GAAGAC(2/6)
BcnI 714 CCGGG CC^[GC]GG
BmyI 774 GTGCAC G[AGT]GC[ACT]^C
BpuAI 340 GAAGAC GAAGAC(2/6)
BseAI 12 TCCGGA T^CCGGA
BsiHKAI 774 GTGCAC G[AT]GC[AT]^C
BsmAI 739 GAGAC GTCTC(1/5)
BsmFI 483 GGGAC GGGAC(10/14)
Bsp1286I 774 GTGCAC G[AGT]GC[ACT]^C
BspEI 12 TCCGGA T^CCGGA
BsrDI 610 GCAATG GCAATG(2/0)
BstNI 446 CCTGG CC^[AT]GG
BstUI 33 CGCG CG^CG
BstXI 788 CCACTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 256 GGATCT [GA]^GATC[CT]
Bsu36I 263 CCTCAGG CC^T[ACGT]AGG
DsaI 474 CCATGG C^C[GA][CT]GG
EarI 40 GAAGAG CTCTTC(1/4)
Eco81I 263 CCTCAGG CC^T[ACGT]AGG
EcoRII 446 CCTGG ^CC[AT]GG
EcoT14I 474 CCATGG C^C[AT][AT]GG
FauI 687 GCGGG CCCGC(4/6)
HaeIII 444 GGCC GG^CC
HincII 500 GTCAAC GT[CT]^[GA]AC
HindII 500 GTCAAC GT[CT]^[GA]AC
Ksp632I 40 GAAGAG CTCTTC(1/4)
MaeII 498 ACGT A^CGT
MflI 256 GGATCT [GA]^GATC[CT]
MroI 12 TCCGGA T^CCGGA
MslI 789 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 446 CCTGG CC^[AT]GG
MvnI 33 CGCG CG^CG
NaeI 60 GCCGGC GCC^GGC
NciI 714 CCGGG CC^[GC]GG
NcoI 474 CCATGG C^CATGG
NgoMIV 60 GCCGGC G^CCGGC
PflMI 474 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 40 GAAGAGC GCTCTTC(1/4)
StuI 443 AGGCCT AGG^CCT
StyI 474 CCATGG C^C[AT][AT]GG
Tsp45I 358 GTGAC ^GT[GC]AC
Van91I 474 CCATGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 215 CCATGAAAACAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 256 GGATCT [GA]^GATC[CT]
XmnI 817 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 34 GCGG CCGC(-3/-1)
687 GCGG
AcsI 178 GAATTT [GA]^AATT[CT]
207 AAATTC
AfaI 300 GTAC GT^AC
352 GTAC
AlwI 418 GATCC GGATC(4/5)
256 GGATC
ApoI 178 GAATTT [GA]^AATT[CT]
207 AAATTC
AvaII 484 GGACC G^G[AT]CC
690 GGTCC
BbvI 71 GCAGC GCAGC(8/12)
724 GCTGC
BfaI 47 CTAG C^TAG
167 CTAG
BsaWI 12 TCCGGA [AT]^CCGG[AT]
148 ACCGGA
BseRI 55 GAGGAG GAGGAG(10/8)
643 GAGGAG
BsrFI 5 ACCGGC [GA]^CCGG[CT]
60 GCCGGC
BsrI 253 ACTGG ACTGG(1/-1)
766 CCAGT
BssKI 446 CCTGG ^CC[ACGT]GG
714 CCGGG
BstEII 357 GGTGACC G^GT[ACGT]ACC
469 GGTTACC
BstPI 357 GGTGACC G^GT[ACGT]ACC
469 GGTTACC
Cfr10I 5 ACCGGC [GA]^CCGG[CT]
60 GCCGGC
Cfr13I 484 GGACC G^G[ACGT]CC
690 GGTCC
EcoO65I 357 GGTGACC G^GT[ACGT]ACC
469 GGTTACC
Fnu4HI 71 GCAGC GC^[ACGT]GC
724 GCTGC
HphI 357 GGTGA GGTGA(8/7)
762 TCACC
ItaI 71 GCAGC GC^[ACGT]GC
724 GCTGC
MaeI 47 CTAG C^TAG
167 CTAG
MaeIII 358 GTGAC ^GT[ACGT]AC
470 GTTAC
MseI 205 TTAA T^TAA
635 TTAA
MwoI 566 GCCTTGATTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
727 GCTGAGAAGGC
RsaI 300 GTAC GT^AC
352 GTAC
Sau96I 484 GGACC G^G[ACGT]CC
690 GGTCC
ScrFI 446 CCTGG CC^[ACGT]GG
714 CCGGG
Tru9I 205 TTAA T^TAA
635 TTAA
TseI 71 GCAGC G^C[AT]GC
724 GCTGC
TspRI 777 CACTG CA[GC]TG[ACGT][ACGT]^
789 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI BsaJI BsiYI BslI BstF5I
Cac8I DdeI DpnII FokI HapII
HinfI HpaII MboI MboII MnlI
MspI NdeII NlaIII NlaIV Sau3AI
SfaNI TfiI Tsp509I