Restriction map for
pFN21AB9906
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# NO CUT ENZYMES ( 111 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BbrPI BcgI
BciVI BclI BfrI BglII BlnI
BsaAI BsaBI BsaI BsaWI BseAI
BseRI BsiHKAI BsiWI BsmBI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraII
Eam1105I Eco47III EcoO109I EcoO65I EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI MamI MfeI MluI
MroI MslI MunI NaeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PstI
PvuI RsrII SacI SalI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI TaqI
Tth111I TthHB8I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcyI 70 GGCGCC G[GA]^CG[CT]C
AflIII 1178 ACATGT A^C[GA][CT]GT
AocI 334 CCTCAGG CC^T[ACGT]AGG
BalI 629 TGGCCA TGG^CCA
BbeI 70 GGCGCC GGCGC^C
BbsI 123 GAAGAC GAAGAC(2/6)
BglI 73 GCCCCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 349 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 349 GCTCAGC GC^T[ACGT]AGC
BpuAI 123 GAAGAC GAAGAC(2/6)
BsaHI 70 GGCGCC G[GA]^CG[CT]C
BsiEI 80 CGGCCG CG[GA][CT]^CG
BsmI 1127 GAATGC GAATGC(1/-1)
Bsp1407I 824 TGTACA T^GTACA
BspLU11I 1178 ACATGT A^CATGT
BspMI 466 GCAGGT ACCTGC(4/8)
BsrGI 824 TGTACA T^GTACA
BstXI 1016 CCAGCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 334 CCTCAGG CC^T[ACGT]AGG
CelII 349 GCTCAGC GC^T[ACGT]AGC
DraI 205 TTTAAA TTT^AAA
DraIII 1517 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 461 GACTTGCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 80 CGGCCG C^GGCCG
EarI 1488 CTCTTC CTCTTC(1/4)
EclXI 80 CGGCCG C^GGCCG
Eco52I 80 CGGCCG C^GGCCG
Eco81I 334 CCTCAGG CC^T[ACGT]AGG
EcoNI 1093 CCTGGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1723 GATATC GAT^ATC
FauI 76 CCCGC CCCGC(4/6)
Hin1I 70 GGCGCC G[GA]^CG[CT]C
HincII 926 GTCAAC GT[CT]^[GA]AC
HindII 926 GTCAAC GT[CT]^[GA]AC
KasI 70 GGCGCC G^GCGCC
Ksp632I 1488 CTCTTC CTCTTC(1/4)
MluNI 629 TGGCCA TGG^CCA
MscI 629 TGGCCA TGG^CCA
NarI 70 GGCGCC GG^CGCC
NcoI 967 CCATGG C^CATGG
NdeI 679 CATATG CA^TATG
NotI 79 GCGGCCGC GC^GGCCGC
PvuII 365 CAGCTG CAG^CTG
SapI 1487 GCTCTTC GCTCTTC(1/4)
SfoI 70 GGCGCC GGC^GCC
SmaI 1431 CCCGGG CCC^GGG
SmlI 270 CTTGAG C^T[CT][GA]AG
SphI 590 GCATGC GCATG^C
SspBI 824 TGTACA T^GTACA
XmaCI 1431 CCCGGG C^CCGGG
XmaI 1431 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 28 enzymes) #
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AccII 78 CGCG CG^CG
93 CGCG
AclI 200 AACGTT AA^CGTT
711 AACGTT
AvaI 157 CCCGAG C^[CT]CG[GA]G
1431 CCCGGG
BanI 70 GGCGCC G^G[CT][GA]CC
1089 GGTGCC
BbvCI 1282 CCTCAGC CCTCAGC(-5/-2)
1612 CCTCAGC
BsgI 857 CTGCAC GTGCAG(16/14)
1241 CTGCAC
BsmFI 459 GGGAC GGGAC(10/14)
1535 GTCCC
BsoBI 157 CCCGAG C^[CT]CG[GA]G
1431 CCCGGG
BspHI 107 TCATGA T^CATGA
288 TCATGA
BstUI 78 CGCG CG^CG
93 CGCG
BstYI 951 AGATCC [GA]^GATC[CT]
1504 GGATCT
EcoRI 145 GAATTC G^AATTC
284 GAATTC
HaeII 46 AGCGCC [GA]GCGC^[CT]
70 GGCGCC
KspI 77 CCGCGG CCGC^GG
92 CCGCGG
MaeII 201 ACGT A^CGT
712 ACGT
MflI 951 AGATCC [GA]^GATC[CT]
1504 GGATCT
MvnI 78 CGCG CG^CG
93 CGCG
PflMI 561 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCAATCCATGG
PleI 273 GAGTC GAGTC(4/5)
516 GAGTC
Psp1406I 200 AACGTT AA^CGTT
711 AACGTT
RcaI 107 TCATGA T^CATGA
288 TCATGA
SacII 77 CCGCGG CCGC^GG
92 CCGCGG
ScaI 1042 AGTACT AGT^ACT
1069 AGTACT
SfcI 511 CTACAG C^T[GA][CT]AG
1618 CTACAG
StuI 331 AGGCCT AGG^CCT
1382 AGGCCT
Tsp45I 115 GTCAC ^GT[GC]AC
1215 GTCAC
Van91I 561 CCAGATCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
962 CCAATCCATGG
XhoII 951 AGATCC [GA]^GATC[CT]
1504 GGATCT
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BsiYI
BslI BsmAI Bsp1286I BsrDI BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TfiI Tru9I
TseI Tsp509I TspRI