Restriction map for
pFN21AB9906
(Circular) (Six-base) MAPSORT of: pFN21AB9906.seq Check: 9501 from: 1 to: 6472
pFN21AB9906 6472 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4282 278
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3815 1937
Size: 1878 4594
Acc65I G'GTAC_C
Cuts at: 3793 3793
Size: 6472
AclI AA'CG_TT
Cuts at: 1563 2206 2717 4599 4972 1563
Size: 643 511 1882 373 3063
Fragments arranged by size:
3063 1882 643 511 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3578 4535 5919 6187 1632
Size: 1946 957 1384 268 1917
Fragments arranged by size:
1946 1917 1384 957 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6275
AflIII A'CryG_T
Cuts at: 1203 3183 5371 6348 1203
Size: 1980 2188 977 1327
Fragments arranged by size:
2188 1980 1327 977
AgeI A'CCGG_T
Cuts at: 5303 5303
Size: 6472
AhdI GACnn_n'nnGTC
Cuts at: 5201 5201
Size: 6472
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6472
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6472
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2292 2324 2292
Size: 32 6440
AlwNI CAG_nnn'CTG
Cuts at: 4215 5787 4215
Size: 1572 4900
ApaLI G'TGCA_C
Cuts at: 4529 5685 4529
Size: 1156 5316
ApoI r'AATT_y
Cuts at: 1493 2150 2253 2289 3088 3781 4007 4159 6268
Size: 657 103 36 799 693 226 152 2109
Cuts at: 6268 1493
Size: 1697
Fragments arranged by size:
2109 1697 799 693 657 226 152 103
36
AseI AT'TA_AT
Cuts at: 160 1032 5026 160
Size: 872 3994 1606
Fragments arranged by size:
3994 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
AvaI C'yCGr_G
Cuts at: 1870 1943 2162 3436 3797 1870
Size: 73 219 1274 361 4545
Fragments arranged by size:
4545 1274 361 219 73
BamHI G'GATC_C
Cuts at: 3802 4169 3802
Size: 367 6105
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 3094 3793 5248 618
Size: 325 217 627 288 1019 699 1455 1842
Fragments arranged by size:
1842 1455 1019 699 627 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2042 2106 2980 3761 3791 1391
Size: 651 64 874 781 30 4072
Fragments arranged by size:
4072 874 781 651 64 30
BbeI G_GCGC'C
Cuts at: 2079 2079
Size: 6472
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2135 928
Size: 971 236 5265
Fragments arranged by size:
5265 971 236
BbvCI CC'TCA_GC
Cuts at: 3288 3618 3288
Size: 330 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4663 4697 1903
Size: 34 2726 34 3678
Fragments arranged by size:
3678 2726 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4364 5574 4364
Size: 1210 5262
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6472
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2084 5083 136
Size: 107 122 71 631 605 412 2999 1525
Fragments arranged by size:
2999 1525 631 605 412 122 107 71
BglII A'GATC_T
Cuts at: 6467 6467
Size: 6472
BlpI GC'TnA_GC
Cuts at: 2355 3889 2355
Size: 1534 4938
Bme1580I G_kGCm'C
Cuts at: 1790 2163 4533 5689 1790
Size: 373 2370 1156 2573
Fragments arranged by size:
2573 2370 1156 373
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6472
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3764 4203 5161
Size: 416 53 83 399 24 2347 439 958
Cuts at: 5161 442
Size: 1753
Fragments arranged by size:
2347 1753 958 439 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2188 2188
Size: 6472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3120 3507 3627 5132 1239
Size: 1881 387 120 1505 2579
Fragments arranged by size:
2579 1881 1505 387 120
Bpu10I CC'TnA_GC
Cuts at: 3288 3618 3288
Size: 330 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2296 4597 5462 5760 2296
Size: 2301 865 298 3008
Fragments arranged by size:
3008 2301 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5135 882
Size: 617 3636 2219
Fragments arranged by size:
3636 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4263 493
Size: 711 3059 2702
Fragments arranged by size:
3059 2702 711
BsaBI GATnn'nnATC
Cuts at: 6466 6466
Size: 6472
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2076 4279 4661 5296
Size: 53 83 186 936 543 2203 382 635
Cuts at: 5296 275
Size: 1451
Fragments arranged by size:
2203 1451 936 635 543 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4905 5303 5577 5724 6135 4905
Size: 398 274 147 411 5242
Fragments arranged by size:
5242 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3300 3330 1178
Size: 30 165 30 1897 30 4320
Fragments arranged by size:
4320 1897 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6472
BseYI C'CCAG_C
Cuts at: 3762 5675 6171 3762
Size: 1913 496 4063
Fragments arranged by size:
4063 1913 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2847 3231 1163
Size: 573 1111 384 4404
Fragments arranged by size:
4404 1111 573 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2088 3922 4683 5711 1540
Size: 275 186 87 1834 761 1028 2301
Fragments arranged by size:
2301 1834 1028 761 275 186 87
BsiHKAI G_wGCw'C
Cuts at: 4533 4618 5689 4533
Size: 85 1071 5316
Fragments arranged by size:
5316 1071 85
BsmI GAATG_Cn'
Cuts at: 3138 3983 4076 3138
Size: 845 93 5534
Fragments arranged by size:
5534 845 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2042 2106 2163 2980 3761 3791 4533
Size: 399 252 64 57 817 781 30 742
Cuts at: 4533 4618 5689 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 817 781 742 399 252 85
64 57 30
BspEI T'CCGG_A
Cuts at: 6135 6135
Size: 6472
BspHI T'CATG_A
Cuts at: 2112 2293 4361 6091 2112
Size: 181 2068 1730 2493
Fragments arranged by size:
2493 2068 1730 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2462 3827 844
Size: 1618 1365 3489
Fragments arranged by size:
3489 1618 1365
BsrBI CCG'CTC
Cuts at: 1394 1823 4359 1394
Size: 429 2536 3507
Fragments arranged by size:
3507 2536 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2386 2748 3369 3387 3678 4967 5141
Size: 1183 1138 362 621 18 291 1289 174
Cuts at: 5141 65
Size: 1396
Fragments arranged by size:
1396 1289 1183 1138 621 362 291 174
18
BsrFI r'CCGG_y
Cuts at: 1700 1810 5116 5303 1700
Size: 110 3306 187 2869
Fragments arranged by size:
3306 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2829 96
Size: 2733 3739
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6472
BssSI C'ACGA_G
Cuts at: 4532 5544 4532
Size: 1012 5460
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6472
BstBI TT'CG_AA
Cuts at: 5276 5276
Size: 6472
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6472
BstXI CCAn_nnnn'nTGG
Cuts at: 3028 3028
Size: 6472
BstYI r'GATC_y
Cuts at: 1972 2956 3509 3802 4169 4554 4571 6023 6109
Size: 984 553 293 367 385 17 1452 86
Cuts at: 6109 6121 6467 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 984 553 385 367 346 293
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2340 2340
Size: 6472
BtgI C'CryG_G
Cuts at: 513 1065 2004 2082 2097 2972 513
Size: 552 939 78 15 875 4013
Fragments arranged by size:
4013 939 875 552 78 15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5733
Fragments arranged by size:
5733 683 56
BtsI GCAGTG_nn'
Cuts at: 3015 3988 4782 4802 3015
Size: 973 794 20 4685
Fragments arranged by size:
4685 973 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6472
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6437
DraI TTT'AAA
Cuts at: 2212 3776 2212
Size: 1564 4908
DraIII CAC_nnn'GTG
Cuts at: 3527 3527
Size: 6472
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2472 5479 809
Size: 507 1156 3007 1802
Fragments arranged by size:
3007 1802 1156 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2085 2634
Size: 54 1425 50 275 18 177 78 549
Cuts at: 2634 3919 4808 6437 8
Size: 1285 889 1629 43
Fragments arranged by size:
1629 1425 1285 889 549 275 177 78
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2085 3919 1537
Size: 275 273 1834 4090
Fragments arranged by size:
4090 1834 275 273
EarI CTCTTCn'nnn_
Cuts at: 1353 3499 4402 1353
Size: 2146 903 3423
Fragments arranged by size:
3423 2146 903
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5059 5433 5579 387
Size: 1064 138 3470 374 146 1280
Fragments arranged by size:
3470 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3120 3507 3578 3627 4535 5132 5919
Size: 393 1488 387 71 49 908 597 787
Cuts at: 5919 6187 1239
Size: 268 1524
Fragments arranged by size:
1524 1488 908 787 597 393 387 268
71 49
EcoNI CCTnn'n_nnAGG
Cuts at: 3102 3102
Size: 6472
EcoRI G'AATT_C
Cuts at: 2150 2289 3781 4159 2150
Size: 139 1492 378 4463
Fragments arranged by size:
4463 1492 378 139
EcoRV GAT'ATC
Cuts at: 3730 3730
Size: 6472
FspI TGC'GCA
Cuts at: 4978 4978
Size: 6472
HaeII r_GCGC'y
Cuts at: 1678 2055 2079 5619 1678
Size: 377 24 3540 2531
Fragments arranged by size:
3540 2531 377 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3300 3332 3341 3373 3714
Size: 32 135 32 1728 32 9 32 341
Cuts at: 3714 3746 5113 5145 5187 5219 1373
Size: 32 1367 32 42 32 2626
Fragments arranged by size:
2626 1728 1367 341 135 42 32 32
32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 669 1657 1938 2933 3816 4062 669
Size: 988 281 995 883 246 3079
Fragments arranged by size:
3079 995 988 883 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6472
HpaI GTT'AAC
Cuts at: 4062 4062
Size: 6472
KasI G'GCGC_C
Cuts at: 2075 2075
Size: 6472
KpnI G_GTAC'C
Cuts at: 3797 3797
Size: 6472
MfeI C'AATT_G
Cuts at: 4071 4071
Size: 6472
MluI A'CGCG_T
Cuts at: 6348 6348
Size: 6472
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2700 3355 5586 5770 1305
Size: 1395 655 2231 184 2007
Fragments arranged by size:
2231 2007 1395 655 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2636 10
Size: 54 1425 343 804 3846
Fragments arranged by size:
3846 1425 804 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4430 4789 4948 518
Size: 648 3264 359 159 2042
Fragments arranged by size:
3264 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2084 2090 2099 2372 3012 3888 4565 5713
Size: 478 6 9 273 640 876 677 1148
Cuts at: 5713 5958 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 876 677 640 478 273 245
9 6
NarI GG'CG_CC
Cuts at: 2076 2076
Size: 6472
NcoI C'CATG_G
Cuts at: 513 1065 2004 2972 513
Size: 552 939 968 4013
Fragments arranged by size:
4013 968 939 552
NdeI CA'TA_TG
Cuts at: 387 2685 387
Size: 2298 4174
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6472
NotI GC'GGCC_GC
Cuts at: 2085 3919 2085
Size: 1834 4638
NspI r_CATG'y
Cuts at: 1132 1229 2599 2830 3187 3830 5375 1132
Size: 97 1370 231 357 643 1545 2229
Fragments arranged by size:
2229 1545 1370 643 357 231 97
PasI CC'CwG_GG
Cuts at: 1348 2801 3449 1348
Size: 1453 648 4371
Fragments arranged by size:
4371 1453 648
PciI A'CATG_T
Cuts at: 3183 5371 3183
Size: 2188 4284
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 2973 1108
Size: 1464 401 4607
Fragments arranged by size:
4607 1464 401
PmeI GTTT'AAAC
Cuts at: 3776 3776
Size: 6472
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4541 4573 6054 6086 4541
Size: 32 1481 32 4927
Fragments arranged by size:
4927 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6472
PsiI TTA'TAA
Cuts at: 4042 4042
Size: 6472
PstI C_TGCA'G
Cuts at: 830 3824 830
Size: 2994 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6472
PvuII CAG'CTG
Cuts at: 2372 2372
Size: 6472
SacII CC_GC'GG
Cuts at: 2085 2100 2085
Size: 15 6457
SalI G'TCGA_C
Cuts at: 1936 3814 1936
Size: 1878 4594
SapI GCTCTTCn'nnn_
Cuts at: 3499 3499
Size: 6472
SbfI CC_TGCA'GG
Cuts at: 3824 3824
Size: 6472
ScaI AGT'ACT
Cuts at: 3049 3076 4720 6432 3049
Size: 27 1644 1712 3089
Fragments arranged by size:
3089 1712 1644 27
SfcI C'TryA_G
Cuts at: 826 1045 2516 3623 3820 4955 5636 5827 826
Size: 219 1471 1107 197 1135 681 191 1471
Fragments arranged by size:
1471 1471 1135 1107 681 219 197 191
SfoI GGC'GCC
Cuts at: 2077 2077
Size: 6472
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6472
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6472
SmaI CCC'GGG
Cuts at: 1872 3438 3799 1872
Size: 1566 361 4545
Fragments arranged by size:
4545 1566 361
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2275 4576 5477 5739 820
Size: 197 926 332 2301 901 262 1553
Fragments arranged by size:
2301 1553 926 901 332 262 197
SnaBI TAC'GTA
Cuts at: 493 4263 493
Size: 3770 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6472
SphI G_CATG'C
Cuts at: 1132 2599 3830 1132
Size: 1467 1231 3774
Fragments arranged by size:
3774 1467 1231
SspI AAT'ATT
Cuts at: 5 52 4396 5
Size: 47 4344 2081
Fragments arranged by size:
4344 2081 47
StuI AGG'CCT
Cuts at: 1517 2338 3389 1517
Size: 821 1051 4600
Fragments arranged by size:
4600 1051 821
StyI C'CwwG_G
Cuts at: 513 1065 2004 2458 2898 2972 3911 513
Size: 552 939 454 440 74 939 3074
Fragments arranged by size:
3074 939 939 552 454 440 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2787 3020 4499 4516 4669 4854 5299
Size: 1255 1207 233 1479 17 153 185 445
Cuts at: 5299 325
Size: 1498
Fragments arranged by size:
1498 1479 1255 1207 445 233 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2829 3047 3074
Size: 274 80 33 51 1443 852 218 27
Cuts at: 3074 4718 6430 96
Size: 1644 1712 138
Fragments arranged by size:
1712 1644 1443 852 274 218 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6432 6464 6432
Size: 32 6440
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6472
XbaI T'CTAG_A
Cuts at: 3808 3808
Size: 6472
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6472
XmaI C'CCGG_G
Cuts at: 1870 3436 3797 1870
Size: 1566 361 4545
Fragments arranged by size:
4545 1566 361
XmnI GAAnn'nnTTC
Cuts at: 4601 4601
Size: 6472
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4280 276
Size: 53 83 186 936 2746 2468
Fragments arranged by size:
2746 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoO109I FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SanDI SexAI SfiI SrfI
SwaI XcmI