Restriction map for
pFN21AB9906
(Linear) (Six-base) MAPSORT of: KIBB9906.seq Check: 3756 from: 1 to: 1767
KIBB9906.orf
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AclI AA'CG_TT
Cuts at: 0 201 712 1767
Size: 201 511 1055
Fragments arranged by size:
1055 511 201
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1573 1767
Size: 1573 194
AflIII A'CryG_T
Cuts at: 0 1178 1767
Size: 1178 589
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 287 319 1767
Size: 287 32 1448
Fragments arranged by size:
1448 287 32
ApoI r'AATT_y
Cuts at: 0 145 248 284 1083 1767
Size: 145 103 36 799 684
Fragments arranged by size:
799 684 145 103 36
AvaI C'yCGr_G
Cuts at: 0 157 1431 1767
Size: 157 1274 336
Fragments arranged by size:
1274 336 157
BanI G'GyrC_C
Cuts at: 0 70 1089 1767
Size: 70 1019 678
Fragments arranged by size:
1019 678 70
BanII G_rGCy'C
Cuts at: 0 37 101 975 1756 1767
Size: 37 64 874 781 11
Fragments arranged by size:
874 781 64 37 11
BbeI G_GCGC'C
Cuts at: 0 74 1767
Size: 74 1693
BbsI GAAGACnn'nnnn_
Cuts at: 0 130 1767
Size: 130 1637
BbvCI CC'TCA_GC
Cuts at: 0 1283 1613 1767
Size: 1283 330 154
Fragments arranged by size:
1283 330 154
BglI GCCn_nnn'nGGC
Cuts at: 0 79 1767
Size: 79 1688
BlpI GC'TnA_GC
Cuts at: 0 350 1767
Size: 350 1417
Bme1580I G_kGCm'C
Cuts at: 0 158 1767
Size: 158 1609
BmrI ACTGGGnnnn_n'
Cuts at: 0 1759 1767
Size: 1759 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 183 1767
Size: 183 1584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1502 1622 1767
Size: 1115 387 120 145
Fragments arranged by size:
1115 387 145 120
Bpu10I CC'TnA_GC
Cuts at: 0 1283 1613 1767
Size: 1283 330 154
Fragments arranged by size:
1283 330 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 1767
Size: 291 1476
BsaHI Gr'CG_yC
Cuts at: 0 71 1767
Size: 71 1696
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1295 1325 1767
Size: 1295 30 442
Fragments arranged by size:
1295 442 30
BseYI C'CCAG_C
Cuts at: 0 1757 1767
Size: 1757 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 842 1226 1767
Size: 842 384 541
Fragments arranged by size:
842 541 384
BsiEI CG_ry'CG
Cuts at: 0 83 1767
Size: 83 1684
BsmI GAATG_Cn'
Cuts at: 0 1133 1767
Size: 1133 634
Bsp1286I G_dGCh'C
Cuts at: 0 37 101 158 975 1756 1767
Size: 37 64 57 817 781 11
Fragments arranged by size:
817 781 64 57 37 11
BspHI T'CATG_A
Cuts at: 0 107 288 1767
Size: 107 181 1479
Fragments arranged by size:
1479 181 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 457 1767
Size: 457 1310
BsrDI GCAATG_nn'
Cuts at: 0 381 743 1364 1382 1673 1767
Size: 381 362 621 18 291 94
Fragments arranged by size:
621 381 362 291 94 18
BsrGI T'GTAC_A
Cuts at: 0 824 1767
Size: 824 943
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1023 1767
Size: 1023 744
BstYI r'GATC_y
Cuts at: 0 951 1504 1767
Size: 951 553 263
Fragments arranged by size:
951 553 263
Bsu36I CC'TnA_GG
Cuts at: 0 335 1767
Size: 335 1432
BtgI C'CryG_G
Cuts at: 0 77 92 967 1767
Size: 77 15 875 800
Fragments arranged by size:
875 800 77 15
BtsI GCAGTG_nn'
Cuts at: 0 1010 1767
Size: 1010 757
DraI TTT'AAA
Cuts at: 0 207 1767
Size: 207 1560
DraIII CAC_nnn'GTG
Cuts at: 0 1522 1767
Size: 1522 245
DrdI GACnn_nn'nnGTC
Cuts at: 0 467 1767
Size: 467 1300
EaeI y'GGCC_r
Cuts at: 0 2 80 629 1767
Size: 2 78 549 1138
Fragments arranged by size:
1138 549 78 2
EagI C'GGCC_G
Cuts at: 0 80 1767
Size: 80 1687
EarI CTCTTCn'nnn_
Cuts at: 0 1494 1767
Size: 1494 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1115 1502 1573 1622 1767
Size: 1115 387 71 49 145
Fragments arranged by size:
1115 387 145 71 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1097 1767
Size: 1097 670
EcoRI G'AATT_C
Cuts at: 0 145 284 1767
Size: 145 139 1483
Fragments arranged by size:
1483 145 139
EcoRV GAT'ATC
Cuts at: 0 1725 1767
Size: 1725 42
HaeII r_GCGC'y
Cuts at: 0 50 74 1767
Size: 50 24 1693
Fragments arranged by size:
1693 50 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1295 1327 1336 1368 1709 1741 1767
Size: 1295 32 9 32 341 32 26
Fragments arranged by size:
1295 341 32 32 32 26 9
HincII GTy'rAC
Cuts at: 0 928 1767
Size: 928 839
KasI G'GCGC_C
Cuts at: 0 70 1767
Size: 70 1697
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 695 1350 1767
Size: 695 655 417
Fragments arranged by size:
695 655 417
MscI TGG'CCA
Cuts at: 0 631 1767
Size: 631 1136
MspA1I CmG'CkG
Cuts at: 0 79 85 94 367 1007 1767
Size: 79 6 9 273 640 760
Fragments arranged by size:
760 640 273 79 9 6
NarI GG'CG_CC
Cuts at: 0 71 1767
Size: 71 1696
NcoI C'CATG_G
Cuts at: 0 967 1767
Size: 967 800
NdeI CA'TA_TG
Cuts at: 0 680 1767
Size: 680 1087
NotI GC'GGCC_GC
Cuts at: 0 80 1767
Size: 80 1687
NspI r_CATG'y
Cuts at: 0 594 825 1182 1767
Size: 594 231 357 585
Fragments arranged by size:
594 585 357 231
PasI CC'CwG_GG
Cuts at: 0 796 1444 1767
Size: 796 648 323
Fragments arranged by size:
796 648 323
PciI A'CATG_T
Cuts at: 0 1178 1767
Size: 1178 589
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 968 1767
Size: 567 401 799
Fragments arranged by size:
799 567 401
PvuII CAG'CTG
Cuts at: 0 367 1767
Size: 367 1400
SacII CC_GC'GG
Cuts at: 0 80 95 1767
Size: 80 15 1672
Fragments arranged by size:
1672 80 15
SapI GCTCTTCn'nnn_
Cuts at: 0 1494 1767
Size: 1494 273
ScaI AGT'ACT
Cuts at: 0 1044 1071 1767
Size: 1044 27 696
Fragments arranged by size:
1044 696 27
SfcI C'TryA_G
Cuts at: 0 511 1618 1767
Size: 511 1107 149
Fragments arranged by size:
1107 511 149
SfoI GGC'GCC
Cuts at: 0 72 1767
Size: 72 1695
SmaI CCC'GGG
Cuts at: 0 1433 1767
Size: 1433 334
SmlI C'TyrA_G
Cuts at: 0 270 1767
Size: 270 1497
SphI G_CATG'C
Cuts at: 0 594 1767
Size: 594 1173
StuI AGG'CCT
Cuts at: 0 333 1384 1767
Size: 333 1051 383
Fragments arranged by size:
1051 383 333
StyI C'CwwG_G
Cuts at: 0 453 893 967 1767
Size: 453 440 74 800
Fragments arranged by size:
800 453 440 74
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 782 1015 1767
Size: 782 233 752
Fragments arranged by size:
782 752 233
TatI w'GTAC_w
Cuts at: 0 824 1042 1069 1767
Size: 824 218 27 698
Fragments arranged by size:
824 698 218 27
XmaI C'CCGG_G
Cuts at: 0 1431 1767
Size: 1431 336
Enzymes that do cut:
AclI AcuI AflIII AloI ApoI AvaI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaHI BsaXI BseYI BsgI BsiEI
BsmI Bsp1286I BspHI BspMI BsrDI BsrGI BstXI BstYI
Bsu36I BtgI BtsI DraI DraIII DrdI EaeI EagI
EarI Eco57MI EcoNI EcoRI EcoRV HaeII Hin4I HincII
KasI MmeI MscI MspA1I NarI NcoI NdeI NotI
NspI PasI PciI PflMI PvuII SacII SapI ScaI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BsaI BsaAI BsaBI BsaWI BseRI BsiHKAI
BsiWI BsmBI BspEI BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI EciI EcoICRI
EcoO109I FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI MslI NaeI NgoMIV NheI NruI NsiI
PacI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TstI Tth111I XbaI XcmI XhoI
XmnI ZraI