Restriction map for
pFN21AB6992
(Circular) (Six-base) MAPSORT of: pFN21AB6992.seq Check: 5168 from: 1 to: 5461
pFN21AB6992 5461 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3271 278
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2804 1937
Size: 867 4594
Acc65I G'GTAC_C
Cuts at: 2782 2782
Size: 5461
AclI AA'CG_TT
Cuts at: 1563 3588 3961 1563
Size: 2025 373 3063
Fragments arranged by size:
3063 2025 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3524 4908 5176 1632
Size: 1892 1384 268 1917
Fragments arranged by size:
1917 1892 1384 268
AfeI AGC'GCT
Cuts at: 2182 2487 2182
Size: 305 5156
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5264
AflIII A'CryG_T
Cuts at: 1203 4360 5337 1203
Size: 3157 977 1327
Fragments arranged by size:
3157 1327 977
AgeI A'CCGG_T
Cuts at: 4292 4292
Size: 5461
AhdI GACnn_n'nnGTC
Cuts at: 4190 4190
Size: 5461
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5461
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5461
AlwNI CAG_nnn'CTG
Cuts at: 3204 4776 3204
Size: 1572 3889
ApaI G_GGCC'C
Cuts at: 2317 2317
Size: 5461
ApaLI G'TGCA_C
Cuts at: 3518 4674 3518
Size: 1156 4305
ApoI r'AATT_y
Cuts at: 1493 2770 2996 3148 5257 1493
Size: 1277 226 152 2109 1697
Fragments arranged by size:
2109 1697 1277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4015 160
Size: 872 2983 1606
Fragments arranged by size:
2983 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
AvaI C'yCGr_G
Cuts at: 1870 1943 2385 2786 1870
Size: 73 442 401 4545
Fragments arranged by size:
4545 442 401 73
BamHI G'GATC_C
Cuts at: 2791 3158 2791
Size: 367 5094
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2272 2782 4237 618
Size: 325 217 627 485 510 1455 1842
Fragments arranged by size:
1842 1455 627 510 485 325 217
BanII G_rGCy'C
Cuts at: 1391 2317 2393 2780 1391
Size: 926 76 387 4072
Fragments arranged by size:
4072 926 387 76
BbeI G_GCGC'C
Cuts at: 2276 2276
Size: 5461
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4490
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3652 3686 1903
Size: 34 1715 34 3678
Fragments arranged by size:
3678 1715 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3353 4563 3353
Size: 1210 4251
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5461
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4072 136
Size: 107 122 71 631 605 2400 1525
Fragments arranged by size:
2400 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5456 5456
Size: 5461
BlpI GC'TnA_GC
Cuts at: 2065 2878 2065
Size: 813 4648
Bme1580I G_kGCm'C
Cuts at: 1790 2317 3522 4678 1790
Size: 527 1205 1156 2573
Fragments arranged by size:
2573 1205 1156 527
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5461
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2298 3192 4150
Size: 416 53 83 399 24 881 894 958
Cuts at: 4150 442
Size: 1753
Fragments arranged by size:
1753 958 894 881 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5461
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4121 1239
Size: 2882 2579
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5461
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3586 4451 4749 3586
Size: 865 298 4298
Fragments arranged by size:
4298 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4124 882
Size: 617 2625 2219
Fragments arranged by size:
2625 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3252 493
Size: 711 2048 2702
Fragments arranged by size:
2702 2048 711
BsaBI GATnn'nnATC
Cuts at: 5455 5455
Size: 5461
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2273 3268 3650 4285
Size: 53 83 186 936 740 995 382 635
Cuts at: 4285 275
Size: 1451
Fragments arranged by size:
1451 995 936 740 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3894 4292 4566 4713 5124 3894
Size: 398 274 147 411 4231
Fragments arranged by size:
4231 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2550 2580 1178
Size: 30 165 30 1147 30 4059
Fragments arranged by size:
4059 1147 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5461
BseYI C'CCAG_C
Cuts at: 4664 5160 4664
Size: 496 4965
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4888
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2143 2171 2911 3672 4700 1540
Size: 275 186 142 28 740 761 1028 2301
Fragments arranged by size:
2301 1028 761 740 275 186 142 28
BsiHKAI G_wGCw'C
Cuts at: 2370 3522 3607 4678 2370
Size: 1152 85 1071 3153
Fragments arranged by size:
3153 1152 1071 85
BsmI GAATG_Cn'
Cuts at: 2972 3065 2972
Size: 93 5368
BsmBI CGTCTCn'nnnn_
Cuts at: 2058 2058
Size: 5461
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2317 2370 2393 2780 3522 3607 4678
Size: 399 527 53 23 387 742 85 1071
Cuts at: 4678 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 527 399 387 85 53
23
BspEI T'CCGG_A
Cuts at: 5124 5124
Size: 5461
BspHI T'CATG_A
Cuts at: 3350 5080 3350
Size: 1730 3731
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2816 844
Size: 1972 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3348 1394
Size: 429 1525 3507
Fragments arranged by size:
3507 1525 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3956 4130 65
Size: 1183 2708 174 1396
Fragments arranged by size:
2708 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4105 4292 1700
Size: 110 2295 187 2869
Fragments arranged by size:
2869 2295 187 110
BsrGI T'GTAC_A
Cuts at: 96 2418 96
Size: 2322 3139
BssHII G'CGCG_C
Cuts at: 1925 2396 2398 1925
Size: 471 2 4988
Fragments arranged by size:
4988 471 2
BssSI C'ACGA_G
Cuts at: 3521 4533 3521
Size: 1012 4449
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5461
BstBI TT'CG_AA
Cuts at: 4265 4265
Size: 5461
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5461
BstYI r'GATC_y
Cuts at: 1972 2791 3158 3543 3560 5012 5098 5110 5456
Size: 819 367 385 17 1452 86 12 346
Cuts at: 5456 1972
Size: 1977
Fragments arranged by size:
1977 1452 819 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2253 2253
Size: 5461
BtgI C'CryG_G
Cuts at: 513 1065 2109 2206 2373 2403 2519 513
Size: 552 1044 97 167 30 116 3455
Fragments arranged by size:
3455 1044 552 167 116 97 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2084 487
Size: 683 56 858 3864
Fragments arranged by size:
3864 858 683 56
BtsI GCAGTG_nn'
Cuts at: 2977 3771 3791 2977
Size: 794 20 4647
Fragments arranged by size:
4647 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5461
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5426
DraI TTT'AAA
Cuts at: 2765 2765
Size: 5461
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4468 809
Size: 507 3152 1802
Fragments arranged by size:
3152 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2140 2168 2185
Size: 54 1425 50 275 18 310 28 17
Cuts at: 2185 2908 3797 5426 8
Size: 723 889 1629 43
Fragments arranged by size:
1629 1425 889 723 310 275 54 50
43 28 18 17
EagI C'GGCC_G
Cuts at: 1537 1812 2140 2168 2908 1537
Size: 275 328 28 740 4090
Fragments arranged by size:
4090 740 328 275 28
EarI CTCTTCn'nnn_
Cuts at: 1353 2503 3391 1353
Size: 1150 888 3423
Fragments arranged by size:
3423 1150 888
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4048 4422 4568 387
Size: 1064 138 2459 374 146 1280
Fragments arranged by size:
2459 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3524 4121 4908 5176 1239
Size: 393 1892 597 787 268 1524
Fragments arranged by size:
1892 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2770 3148 2770
Size: 378 5083
FspI TGC'GCA
Cuts at: 3967 3967
Size: 5461
HaeII r_GCGC'y
Cuts at: 1678 2184 2276 2489 4608 1678
Size: 506 92 213 2119 2531
Fragments arranged by size:
2531 2119 506 213 92
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2550 2582 2668 2700 4102
Size: 32 135 32 978 32 86 32 1402
Cuts at: 4102 4134 4176 4208 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1402 978 135 86 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2805 3051 669
Size: 988 281 867 246 3079
Fragments arranged by size:
3079 988 867 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5461
HpaI GTT'AAC
Cuts at: 3051 3051
Size: 5461
KasI G'GCGC_C
Cuts at: 2272 2272
Size: 5461
KpnI G_GTAC'C
Cuts at: 2786 2786
Size: 5461
MfeI C'AATT_G
Cuts at: 3060 3060
Size: 5461
MluI A'CGCG_T
Cuts at: 5337 5337
Size: 5461
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4575 4759 1305
Size: 3270 184 2007
Fragments arranged by size:
3270 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3639
Fragments arranged by size:
3639 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3419 3778 3937 518
Size: 648 2253 359 159 2042
Fragments arranged by size:
2253 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2111 2173 2208 2231 2405 2877 3554 4702
Size: 505 62 35 23 174 472 677 1148
Cuts at: 4702 4947 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 505 472 245 174 62
35 23
NarI GG'CG_CC
Cuts at: 2273 2273
Size: 5461
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4909
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5461
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5461
NotI GC'GGCC_GC
Cuts at: 2908 2908
Size: 5461
NspI r_CATG'y
Cuts at: 1132 1229 2341 2819 4364 1132
Size: 97 1112 478 1545 2229
Fragments arranged by size:
2229 1545 1112 478 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5461
PciI A'CATG_T
Cuts at: 4360 4360
Size: 5461
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5461
PmeI GTTT'AAAC
Cuts at: 2765 2765
Size: 5461
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3530 3562 5043 5075 3530
Size: 32 1481 32 3916
Fragments arranged by size:
3916 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5461
PsiI TTA'TAA
Cuts at: 3031 3031
Size: 5461
PspOMI G'GGCC_C
Cuts at: 2313 2313
Size: 5461
PstI C_TGCA'G
Cuts at: 830 2813 830
Size: 1983 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5461
SacII CC_GC'GG
Cuts at: 2112 2209 2406 2112
Size: 97 197 5167
Fragments arranged by size:
5167 197 97
SalI G'TCGA_C
Cuts at: 1936 2803 1936
Size: 867 4594
SbfI CC_TGCA'GG
Cuts at: 2813 2813
Size: 5461
ScaI AGT'ACT
Cuts at: 3709 5421 3709
Size: 1712 3749
SfcI C'TryA_G
Cuts at: 826 1045 2809 3944 4625 4816 826
Size: 219 1764 1135 681 191 1471
Fragments arranged by size:
1764 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2274 2274
Size: 5461
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5461
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5461
SmaI CCC'GGG
Cuts at: 1872 2387 2788 1872
Size: 515 401 4545
Fragments arranged by size:
4545 515 401
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3565 4466 4728 820
Size: 197 926 1622 901 262 1553
Fragments arranged by size:
1622 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3252 493
Size: 2759 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5461
SphI G_CATG'C
Cuts at: 1132 2341 2819 1132
Size: 1209 478 3774
Fragments arranged by size:
3774 1209 478
SspI AAT'ATT
Cuts at: 5 52 3385 5
Size: 47 3333 2081
Fragments arranged by size:
3333 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5461
StyI C'CwwG_G
Cuts at: 513 1065 2197 2709 2900 513
Size: 552 1132 512 191 3074
Fragments arranged by size:
3074 1132 552 512 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2487 3488 3505 3658 3843 4288
Size: 1255 737 170 1001 17 153 185 445
Cuts at: 4288 325
Size: 1498
Fragments arranged by size:
1498 1255 1001 737 445 185 170 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2216 2418 2602
Size: 274 80 33 51 1443 239 202 184
Cuts at: 2602 3707 5419 96
Size: 1105 1712 138
Fragments arranged by size:
1712 1443 1105 274 239 202 184 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5421 5453 5421
Size: 32 5429
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5461
XbaI T'CTAG_A
Cuts at: 2797 2797
Size: 5461
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5461
XmaI C'CCGG_G
Cuts at: 1870 2385 2786 1870
Size: 515 401 4545
Fragments arranged by size:
4545 515 401
XmnI GAAnn'nnTTC
Cuts at: 2700 3590 2700
Size: 890 4571
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3269 276
Size: 53 83 186 936 1735 2468
Fragments arranged by size:
2468 1735 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI
SanDI SapI SexAI SfiI SrfI SwaI XcmI