(Circular) (Six-base) MAPSORT of: pFN21AB7812.seq Check: 4817 from: 1 to: 6490 pFN21AB7812 6490 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4300 278 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2326 3833 1937 Size: 389 1507 4594 Fragments arranged by size: 4594 1507 389 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2069 3456 4553 5937 6205 1632 Size: 437 1387 1097 1384 268 1917 Fragments arranged by size: 1917 1387 1384 1097 437 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6293 AflIII A'CryG_T Cuts at: 1203 3620 5389 6366 1203 Size: 2417 1769 977 1327 Fragments arranged by size: 2417 1769 1327 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 5219 5219 Size: 6490 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6490 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 4233 5805 4233 Size: 1572 4918 ApaLI G'TGCA_C Cuts at: 4547 5703 4547 Size: 1156 5334 ApoI r'AATT_y Cuts at: 1493 2039 2083 2335 2474 2482 2586 2602 2670 Size: 546 44 252 139 8 104 16 68 Cuts at: 2670 2754 2922 3342 3594 3678 3799 4025 4177 Size: 84 168 420 252 84 121 226 152 Cuts at: 4177 6286 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 546 420 252 252 226 168 152 139 121 104 84 84 68 44 16 8 AseI AT'TA_AT Cuts at: 160 1032 2345 5044 160 Size: 872 1313 2699 1606 Fragments arranged by size: 2699 1606 1313 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 2253 3815 1870 Size: 73 310 1562 4545 Fragments arranged by size: 4545 1562 310 73 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3811 5266 618 Size: 325 217 627 2024 1455 1842 Fragments arranged by size: 2024 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3809 1391 Size: 2418 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2119 928 Size: 971 220 5299 Fragments arranged by size: 5299 971 220 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 2160 1548 Size: 612 5878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5101 136 Size: 107 122 71 631 605 3429 1525 Fragments arranged by size: 3429 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6485 6485 Size: 6490 BlpI GC'TnA_GC Cuts at: 3907 3907 Size: 6490 Bme1580I G_kGCm'C Cuts at: 1790 4551 5707 1790 Size: 2761 1156 2573 Fragments arranged by size: 2761 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6490 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4221 5179 442 Size: 416 53 83 399 24 2804 958 1753 Fragments arranged by size: 2804 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2192 2212 2784 2868 2952 3036 3120 3204 Size: 953 20 572 84 84 84 84 84 Cuts at: 3204 3288 3372 3540 3624 3708 5150 1239 Size: 84 84 168 84 84 1442 2579 Fragments arranged by size: 2579 1442 953 572 168 84 84 84 84 84 84 84 84 84 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4615 5480 5778 4615 Size: 865 298 5327 Fragments arranged by size: 5327 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2250 5153 882 Size: 617 751 2903 2219 Fragments arranged by size: 2903 2219 751 617 BsaAI yAC'GTr Cuts at: 493 1204 4281 493 Size: 711 3077 2702 Fragments arranged by size: 3077 2702 711 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4297 4679 5314 275 Size: 53 83 186 936 2764 382 635 1451 Fragments arranged by size: 2764 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3770 4923 5321 5595 5742 6153 3770 Size: 1153 398 274 147 411 4107 Fragments arranged by size: 4107 1153 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6265 Fragments arranged by size: 6265 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6490 BseYI C'CCAG_C Cuts at: 5693 6189 5693 Size: 496 5994 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2058 3051 1163 Size: 573 322 993 4602 Fragments arranged by size: 4602 993 573 322 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4551 4636 5707 4551 Size: 85 1071 5334 Fragments arranged by size: 5334 1071 85 BsmI GAATG_Cn' Cuts at: 4001 4094 4001 Size: 93 6397 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3809 4551 4636 5707 1391 Size: 399 2019 742 85 1071 2174 Fragments arranged by size: 2174 2019 1071 742 399 85 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3845 844 Size: 3001 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2066 4985 5159 65 Size: 1183 818 2919 174 1396 Fragments arranged by size: 2919 1396 1183 818 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6490 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 4550 5562 4550 Size: 1012 5478 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6490 BstBI TT'CG_AA Cuts at: 5294 5294 Size: 6490 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstYI r'GATC_y Cuts at: 1972 3820 4187 4572 4589 6041 6127 6139 6485 Size: 1848 367 385 17 1452 86 12 346 Cuts at: 6485 1972 Size: 1977 Fragments arranged by size: 1977 1848 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4999 Fragments arranged by size: 4999 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5751 Fragments arranged by size: 5751 683 56 BtsI GCAGTG_nn' Cuts at: 2070 4006 4800 4820 2070 Size: 1936 794 20 3740 Fragments arranged by size: 3740 1936 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6455 DraI TTT'AAA Cuts at: 3226 3478 3730 3794 3226 Size: 252 252 64 5922 Fragments arranged by size: 5922 252 252 64 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5497 809 Size: 507 4181 1802 Fragments arranged by size: 4181 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 2220 2264 Size: 54 1425 50 275 18 201 189 44 Cuts at: 2264 3937 4826 6455 8 Size: 1673 889 1629 43 Fragments arranged by size: 1673 1629 1425 889 275 201 189 54 50 44 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2195 3238 3432 4420 1353 Size: 842 1043 194 988 3423 Fragments arranged by size: 3423 1043 988 842 194 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5077 5451 5597 387 Size: 1064 138 3488 374 146 1280 Fragments arranged by size: 3488 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2192 2212 2784 2868 2952 3036 Size: 393 437 123 20 572 84 84 84 Cuts at: 3036 3120 3204 3288 3372 3456 3540 3624 3708 Size: 84 84 84 84 84 84 84 84 Cuts at: 3708 4553 5150 5937 6205 1239 Size: 845 597 787 268 1524 Fragments arranged by size: 1524 845 787 597 572 437 393 268 123 84 84 84 84 84 84 84 84 84 84 84 20 EcoRI G'AATT_C Cuts at: 2039 2586 2670 3678 3799 4177 2039 Size: 547 84 1008 121 378 4352 Fragments arranged by size: 4352 1008 547 378 121 84 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 5637 1678 Size: 3959 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2152 2184 2196 2228 5131 Size: 32 135 32 580 32 12 32 2903 Cuts at: 5131 5163 5205 5237 1373 Size: 32 42 32 2626 Fragments arranged by size: 2903 2626 580 135 42 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3834 4080 669 Size: 988 281 1896 246 3079 Fragments arranged by size: 3079 1896 988 281 246 HindIII A'AGCT_T Cuts at: 748 2883 3219 3303 3387 3555 3723 748 Size: 2135 336 84 84 168 168 3515 Fragments arranged by size: 3515 2135 336 168 168 84 84 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5604 5788 1305 Size: 4299 184 2007 Fragments arranged by size: 4299 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2033 2222 10 Size: 54 1425 343 201 189 4278 Fragments arranged by size: 4278 1425 343 201 189 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 3535 4448 4807 4966 518 Size: 648 1068 1301 913 359 159 2042 Fragments arranged by size: 2042 1301 1068 913 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2080 3906 4583 5731 5976 1606 Size: 474 1826 677 1148 245 2120 Fragments arranged by size: 2120 1826 1148 677 474 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4999 Fragments arranged by size: 4999 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 3624 3848 5393 1132 Size: 97 2395 224 1545 2229 Fragments arranged by size: 2395 2229 1545 224 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6490 PciI A'CATG_T Cuts at: 3620 5389 3620 Size: 1769 4721 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6490 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4559 4591 6072 6104 4559 Size: 32 1481 32 4945 Fragments arranged by size: 4945 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PstI C_TGCA'G Cuts at: 830 3842 830 Size: 3012 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SbfI CC_TGCA'GG Cuts at: 3842 3842 Size: 6490 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SexAI A'CCwGG_T Cuts at: 2121 2121 Size: 6490 SfcI C'TryA_G Cuts at: 826 1045 2113 3838 4973 5654 5845 826 Size: 219 1068 1725 1135 681 191 1471 Fragments arranged by size: 1725 1471 1135 1068 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4594 5495 5757 820 Size: 197 926 2651 901 262 1553 Fragments arranged by size: 2651 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4281 493 Size: 3788 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 2439 4414 5 Size: 47 2387 1975 2081 Fragments arranged by size: 2387 2081 1975 47 StuI AGG'CCT Cuts at: 1517 2189 1517 Size: 672 5818 StyI C'CwwG_G Cuts at: 513 1065 2004 3929 513 Size: 552 939 1925 3074 Fragments arranged by size: 3074 1925 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4517 4534 4687 4872 5317 325 Size: 1255 2937 17 153 185 445 1498 Fragments arranged by size: 2937 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4736 6448 96 Size: 274 80 33 51 1443 2759 1712 138 Fragments arranged by size: 2759 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6450 6482 6450 Size: 32 6458 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6490 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 2522 4619 2522 Size: 2097 4393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4298 276 Size: 53 83 186 936 2764 2468 Fragments arranged by size: 2764 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI