(Circular) (Six-base) MAPSORT of: pFN21AB6984.seq Check: 2864 from: 1 to: 5446 pFN21AB6984 5446 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3256 278 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2688 2700 2789 1937 Size: 751 12 89 4594 Fragments arranged by size: 4594 751 89 12 Acc65I G'GTAC_C Cuts at: 2767 2767 Size: 5446 AclI AA'CG_TT Cuts at: 1563 3573 3946 1563 Size: 2010 373 3063 Fragments arranged by size: 3063 2010 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3509 4893 5161 1632 Size: 1877 1384 268 1917 Fragments arranged by size: 1917 1877 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5249 AflIII A'CryG_T Cuts at: 1203 2550 4345 5322 1203 Size: 1347 1795 977 1327 Fragments arranged by size: 1795 1347 1327 977 AgeI A'CCGG_T Cuts at: 4277 4277 Size: 5446 AhdI GACnn_n'nnGTC Cuts at: 4175 4175 Size: 5446 AleI CACnn'nnGTG Cuts at: 1166 2145 1166 Size: 979 4467 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2209 1184 Size: 1025 4421 AlwNI CAG_nnn'CTG Cuts at: 2408 2485 3189 4761 2408 Size: 77 704 1572 3093 Fragments arranged by size: 3093 1572 704 77 ApaLI G'TGCA_C Cuts at: 3503 4659 3503 Size: 1156 4290 ApoI r'AATT_y Cuts at: 1493 2101 2128 2755 2981 3133 5242 1493 Size: 608 27 627 226 152 2109 1697 Fragments arranged by size: 2109 1697 627 608 226 152 27 AseI AT'TA_AT Cuts at: 160 1032 4000 160 Size: 872 2968 1606 Fragments arranged by size: 2968 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 AvaI C'yCGr_G Cuts at: 1870 1943 2771 1870 Size: 73 828 4545 Fragments arranged by size: 4545 828 73 BamHI G'GATC_C Cuts at: 2776 3143 2776 Size: 367 5079 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2456 2767 4222 618 Size: 325 217 627 669 311 1455 1842 Fragments arranged by size: 1842 1455 669 627 325 311 217 BanII G_rGCy'C Cuts at: 1391 2453 2765 1391 Size: 1062 312 4072 Fragments arranged by size: 4072 1062 312 BbeI G_GCGC'C Cuts at: 2460 2460 Size: 5446 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2315 928 Size: 971 416 4059 Fragments arranged by size: 4059 971 416 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3637 3671 1903 Size: 34 1700 34 3678 Fragments arranged by size: 3678 1700 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3338 4548 3338 Size: 1210 4236 BclI T'GATC_A Cuts at: 1548 1548 Size: 5446 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4057 136 Size: 107 122 71 631 605 2385 1525 Fragments arranged by size: 2385 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5441 5441 Size: 5446 BlpI GC'TnA_GC Cuts at: 2501 2863 2501 Size: 362 5084 Bme1580I G_kGCm'C Cuts at: 1790 2668 3507 4663 1790 Size: 878 839 1156 2573 Fragments arranged by size: 2573 1156 878 839 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5446 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2231 3177 4135 Size: 416 53 83 399 24 814 946 958 Cuts at: 4135 442 Size: 1753 Fragments arranged by size: 1753 958 946 814 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2267 2385 2703 4106 1239 Size: 1028 118 318 1403 2579 Fragments arranged by size: 2579 1403 1028 318 118 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2591 3571 4436 4734 2591 Size: 980 865 298 3303 Fragments arranged by size: 3303 980 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4109 882 Size: 617 2610 2219 Fragments arranged by size: 2610 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2351 3237 493 Size: 711 1147 886 2702 Fragments arranged by size: 2702 1147 886 711 BsaBI GATnn'nnATC Cuts at: 5440 5440 Size: 5446 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2457 3253 3635 4270 Size: 53 83 186 936 924 796 382 635 Cuts at: 4270 275 Size: 1451 Fragments arranged by size: 1451 936 924 796 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3879 4277 4551 4698 5109 3879 Size: 398 274 147 411 4216 Fragments arranged by size: 4216 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2383 2413 1178 Size: 30 165 30 980 30 4211 Fragments arranged by size: 4211 980 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 2335 1186 Size: 925 224 4297 Fragments arranged by size: 4297 925 224 BseYI C'CCAG_C Cuts at: 2433 4649 5145 2433 Size: 2216 496 2734 Fragments arranged by size: 2734 2216 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2373 1163 Size: 573 637 4236 Fragments arranged by size: 4236 637 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2896 3657 4685 1540 Size: 275 186 895 761 1028 2301 Fragments arranged by size: 2301 1028 895 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2232 2453 3507 3592 4663 2232 Size: 221 1054 85 1071 3015 Fragments arranged by size: 3015 1071 1054 221 85 BsmI GAATG_Cn' Cuts at: 2957 3050 2957 Size: 93 5353 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2453 2668 2765 3507 3592 4663 Size: 399 442 221 215 97 742 85 1071 Cuts at: 4663 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 442 399 221 215 97 85 BspEI T'CCGG_A Cuts at: 5109 5109 Size: 5446 BspHI T'CATG_A Cuts at: 3335 5065 3335 Size: 1730 3716 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2801 844 Size: 1957 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3333 1394 Size: 429 1510 3507 Fragments arranged by size: 3507 1510 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2629 3941 4115 65 Size: 1183 1381 1312 174 1396 Fragments arranged by size: 1396 1381 1312 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4090 4277 1700 Size: 110 2280 187 2869 Fragments arranged by size: 2869 2280 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5446 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5446 BssSI C'ACGA_G Cuts at: 3506 4518 3506 Size: 1012 4434 BstAPI GCAn_nnn'nTGC Cuts at: 794 2418 794 Size: 1624 3822 BstBI TT'CG_AA Cuts at: 4250 4250 Size: 5446 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5446 BstXI CCAn_nnnn'nTGG Cuts at: 2147 2147 Size: 5446 BstYI r'GATC_y Cuts at: 1972 2776 3143 3528 3545 4997 5083 5095 5441 Size: 804 367 385 17 1452 86 12 346 Cuts at: 5441 1972 Size: 1977 Fragments arranged by size: 1977 1452 804 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4894 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4707 Fragments arranged by size: 4707 683 56 BtsI GCAGTG_nn' Cuts at: 2191 2442 2486 2962 3756 3776 2191 Size: 251 44 476 794 20 3861 Fragments arranged by size: 3861 794 476 251 44 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5446 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5411 DraI TTT'AAA Cuts at: 2750 2750 Size: 5446 DraIII CAC_nnn'GTG Cuts at: 2418 2418 Size: 5446 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4453 809 Size: 507 3137 1802 Fragments arranged by size: 3137 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411 Size: 54 1425 50 275 18 1063 889 1629 Cuts at: 5411 8 Size: 43 Fragments arranged by size: 1629 1425 1063 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2893 1537 Size: 275 1081 4090 Fragments arranged by size: 4090 1081 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3376 1353 Size: 2023 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2276 4033 4407 4553 387 Size: 1064 138 687 1757 374 146 1280 Fragments arranged by size: 1757 1280 1064 687 374 146 138 EcoICRI GAG'CTC Cuts at: 2451 2451 Size: 5446 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2385 2703 3509 4106 4893 5161 Size: 393 635 118 318 806 597 787 268 Cuts at: 5161 1239 Size: 1524 Fragments arranged by size: 1524 806 787 635 597 393 318 268 118 EcoRI G'AATT_C Cuts at: 2101 2128 2755 3133 2101 Size: 27 627 378 4414 Fragments arranged by size: 4414 627 378 27 FspI TGC'GCA Cuts at: 3952 3952 Size: 5446 HaeII r_GCGC'y Cuts at: 1678 2460 4593 1678 Size: 782 2133 2531 Fragments arranged by size: 2531 2133 782 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4087 4119 4161 4193 1373 Size: 32 135 32 2515 32 42 32 2626 Fragments arranged by size: 2626 2515 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2790 3036 669 Size: 988 281 852 246 3079 Fragments arranged by size: 3079 988 852 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5446 HpaI GTT'AAC Cuts at: 3036 3036 Size: 5446 KasI G'GCGC_C Cuts at: 2456 2456 Size: 5446 KpnI G_GTAC'C Cuts at: 2771 2771 Size: 5446 MfeI C'AATT_G Cuts at: 3045 3045 Size: 5446 MluI A'CGCG_T Cuts at: 5322 5322 Size: 5446 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4560 4744 1305 Size: 3255 184 2007 Fragments arranged by size: 3255 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3624 Fragments arranged by size: 3624 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2145 2213 3404 3763 3922 518 Size: 648 979 68 1191 359 159 2042 Fragments arranged by size: 2042 1191 979 648 359 159 68 MspA1I CmG'CkG Cuts at: 1606 2039 2862 3539 4687 4932 1606 Size: 433 823 677 1148 245 2120 Fragments arranged by size: 2120 1148 823 677 433 245 NarI GG'CG_CC Cuts at: 2457 2457 Size: 5446 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4894 NdeI CA'TA_TG Cuts at: 387 387 Size: 5446 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5446 NotI GC'GGCC_GC Cuts at: 2893 2893 Size: 5446 NspI r_CATG'y Cuts at: 1132 1229 2554 2804 4349 1132 Size: 97 1325 250 1545 2229 Fragments arranged by size: 2229 1545 1325 250 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5446 PciI A'CATG_T Cuts at: 2550 4345 2550 Size: 1795 3651 PflMI CCAn_nnn'nTGG Cuts at: 1108 2408 1108 Size: 1300 4146 PfoI T'CCnGG_A Cuts at: 2244 2244 Size: 5446 PmeI GTTT'AAAC Cuts at: 2750 2750 Size: 5446 PmlI CAC'GTG Cuts at: 2351 2351 Size: 5446 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3515 3547 5028 5060 3515 Size: 32 1481 32 3901 Fragments arranged by size: 3901 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5446 PsiI TTA'TAA Cuts at: 3016 3016 Size: 5446 PstI C_TGCA'G Cuts at: 830 2481 2798 830 Size: 1651 317 3478 Fragments arranged by size: 3478 1651 317 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5446 PvuII CAG'CTG Cuts at: 2039 2039 Size: 5446 SacI G_AGCT'C Cuts at: 2453 2453 Size: 5446 SalI G'TCGA_C Cuts at: 1936 2788 1936 Size: 852 4594 SbfI CC_TGCA'GG Cuts at: 2798 2798 Size: 5446 ScaI AGT'ACT Cuts at: 3694 5406 3694 Size: 1712 3734 SexAI A'CCwGG_T Cuts at: 2115 2115 Size: 5446 SfcI C'TryA_G Cuts at: 826 1045 2477 2794 3929 4610 4801 826 Size: 219 1432 317 1135 681 191 1471 Fragments arranged by size: 1471 1432 1135 681 317 219 191 SfoI GGC'GCC Cuts at: 2458 2458 Size: 5446 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5446 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5446 SmaI CCC'GGG Cuts at: 1872 2773 1872 Size: 901 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2570 3550 4451 4713 820 Size: 197 926 627 980 901 262 1553 Fragments arranged by size: 1553 980 926 901 627 262 197 SnaBI TAC'GTA Cuts at: 493 3237 493 Size: 2744 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5446 SphI G_CATG'C Cuts at: 1132 2804 1132 Size: 1672 3774 SspI AAT'ATT Cuts at: 5 52 3370 5 Size: 47 3318 2081 Fragments arranged by size: 3318 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5446 StyI C'CwwG_G Cuts at: 513 1065 2635 2694 2885 513 Size: 552 1570 59 191 3074 Fragments arranged by size: 3074 1570 552 191 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2164 3473 3490 3643 3828 4273 325 Size: 1255 584 1309 17 153 185 445 1498 Fragments arranged by size: 1498 1309 1255 584 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3692 5404 96 Size: 274 80 33 51 1443 1715 1712 138 Fragments arranged by size: 1715 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 2383 2415 5406 5438 2004 Size: 32 347 32 2991 32 2012 Fragments arranged by size: 2991 2012 347 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5446 XbaI T'CTAG_A Cuts at: 2782 2782 Size: 5446 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2147 2147 Size: 5446 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5446 XmaI C'CCGG_G Cuts at: 1870 2771 1870 Size: 901 4545 XmnI GAAnn'nnTTC Cuts at: 3575 3575 Size: 5446 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3254 276 Size: 53 83 186 936 1720 2468 Fragments arranged by size: 2468 1720 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI