(Circular) (Six-base) MAPSORT of: pFN21AB6973.seq Check: 4509 from: 1 to: 5356 pFN21AB6973 5356 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2348 2348 Size: 5356 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3166 278 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2699 1937 Size: 762 4594 Acc65I G'GTAC_C Cuts at: 2677 2677 Size: 5356 AclI AA'CG_TT Cuts at: 1563 3483 3856 1563 Size: 1920 373 3063 Fragments arranged by size: 3063 1920 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4803 5071 1632 Size: 1787 1384 268 1917 Fragments arranged by size: 1917 1787 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5159 AflIII A'CryG_T Cuts at: 1203 4255 5232 1203 Size: 3052 977 1327 Fragments arranged by size: 3052 1327 977 AgeI A'CCGG_T Cuts at: 4187 4187 Size: 5356 AhdI GACnn_n'nnGTC Cuts at: 4085 4085 Size: 5356 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5356 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5356 AlwNI CAG_nnn'CTG Cuts at: 2218 3099 4671 2218 Size: 881 1572 2903 Fragments arranged by size: 2903 1572 881 ApaI G_GGCC'C Cuts at: 2541 2541 Size: 5356 ApaLI G'TGCA_C Cuts at: 3413 4569 3413 Size: 1156 4200 ApoI r'AATT_y Cuts at: 1493 2191 2665 2891 3043 5152 1493 Size: 698 474 226 152 2109 1697 Fragments arranged by size: 2109 1697 698 474 226 152 AseI AT'TA_AT Cuts at: 160 1032 3910 160 Size: 872 2878 1606 Fragments arranged by size: 2878 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 AvaI C'yCGr_G Cuts at: 1870 1943 2417 2579 2681 1870 Size: 73 474 162 102 4545 Fragments arranged by size: 4545 474 162 102 73 BamHI G'GATC_C Cuts at: 2686 3053 2686 Size: 367 4989 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2638 2677 4132 618 Size: 325 217 627 851 39 1455 1842 Fragments arranged by size: 1842 1455 851 627 325 217 39 BanII G_rGCy'C Cuts at: 1391 2541 2675 1391 Size: 1150 134 4072 Fragments arranged by size: 4072 1150 134 BbeI G_GCGC'C Cuts at: 2642 2642 Size: 5356 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4385 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3547 3581 1903 Size: 34 1610 34 3678 Fragments arranged by size: 3678 1610 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3248 4458 3248 Size: 1210 4146 BclI T'GATC_A Cuts at: 1548 2091 2304 1548 Size: 543 213 4600 Fragments arranged by size: 4600 543 213 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2349 2450 3967 Size: 107 122 71 631 605 677 101 1517 Cuts at: 3967 136 Size: 1525 Fragments arranged by size: 1525 1517 677 631 605 122 107 101 71 BglII A'GATC_T Cuts at: 2370 5351 2370 Size: 2981 2375 BlpI GC'TnA_GC Cuts at: 2462 2773 2462 Size: 311 5045 Bme1580I G_kGCm'C Cuts at: 1790 2541 3417 4573 1790 Size: 751 876 1156 2573 Fragments arranged by size: 2573 1156 876 751 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5356 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2300 3087 4045 Size: 416 53 83 399 24 883 787 958 Cuts at: 4045 442 Size: 1753 Fragments arranged by size: 1753 958 883 787 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5356 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 2225 2411 4016 1239 Size: 863 123 186 1605 2579 Fragments arranged by size: 2579 1605 863 186 123 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3481 4346 4644 3481 Size: 865 298 4193 Fragments arranged by size: 4193 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4019 882 Size: 617 2520 2219 Fragments arranged by size: 2520 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3147 493 Size: 711 1943 2702 Fragments arranged by size: 2702 1943 711 BsaBI GATnn'nnATC Cuts at: 5350 5350 Size: 5356 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2486 2639 3163 3545 Size: 53 83 186 936 953 153 524 382 Cuts at: 3545 4180 275 Size: 635 1451 Fragments arranged by size: 1451 953 936 635 524 382 186 153 83 53 BsaWI w'CCGG_w Cuts at: 3789 4187 4461 4608 5019 3789 Size: 398 274 147 411 4126 Fragments arranged by size: 4126 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5131 Fragments arranged by size: 5131 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2540 1186 Size: 1354 4002 BseYI C'CCAG_C Cuts at: 2014 2549 4559 5055 2014 Size: 535 2010 496 2315 Fragments arranged by size: 2315 2010 535 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2359 1163 Size: 573 623 4160 Fragments arranged by size: 4160 623 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2628 2806 3567 4595 1540 Size: 275 186 627 178 761 1028 2301 Fragments arranged by size: 2301 1028 761 627 275 186 178 BsiHKAI G_wGCw'C Cuts at: 3417 3502 4573 3417 Size: 85 1071 4200 Fragments arranged by size: 4200 1071 85 BsmI GAATG_Cn' Cuts at: 2867 2960 2867 Size: 93 5263 BsmBI CGTCTCn'nnnn_ Cuts at: 2477 2477 Size: 5356 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2541 2675 3417 3502 4573 1391 Size: 399 751 134 742 85 1071 2174 Fragments arranged by size: 2174 1071 751 742 399 134 85 BspEI T'CCGG_A Cuts at: 5019 5019 Size: 5356 BspHI T'CATG_A Cuts at: 3245 4975 3245 Size: 1730 3626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2348 2644 2711 844 Size: 1504 296 67 3489 Fragments arranged by size: 3489 1504 296 67 BsrBI CCG'CTC Cuts at: 1394 1823 3243 1394 Size: 429 1420 3507 Fragments arranged by size: 3507 1420 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3851 4025 65 Size: 1183 2603 174 1396 Fragments arranged by size: 2603 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2488 4000 4187 1700 Size: 110 678 1512 187 2869 Fragments arranged by size: 2869 1512 678 187 110 BsrGI T'GTAC_A Cuts at: 96 2310 96 Size: 2214 3142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5356 BssSI C'ACGA_G Cuts at: 3416 4428 3416 Size: 1012 4344 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5356 BstBI TT'CG_AA Cuts at: 4160 4160 Size: 5356 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5356 BstYI r'GATC_y Cuts at: 1972 2370 2686 3053 3438 3455 4907 4993 5005 Size: 398 316 367 385 17 1452 86 12 Cuts at: 5005 5351 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 398 385 367 346 316 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 2280 513 Size: 552 939 69 207 3589 Fragments arranged by size: 3589 939 552 207 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4617 Fragments arranged by size: 4617 683 56 BtsI GCAGTG_nn' Cuts at: 2872 3666 3686 2872 Size: 794 20 4542 Fragments arranged by size: 4542 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5356 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5321 DraI TTT'AAA Cuts at: 2660 2660 Size: 5356 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2141 4363 809 Size: 507 825 2222 1802 Fragments arranged by size: 2222 1802 825 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2565 2592 Size: 54 1425 50 275 18 612 123 27 Cuts at: 2592 2625 2803 3692 5321 8 Size: 33 178 889 1629 43 Fragments arranged by size: 1629 1425 889 612 275 178 123 54 50 43 33 27 18 EagI C'GGCC_G Cuts at: 1537 1812 2625 2803 1537 Size: 275 813 178 4090 Fragments arranged by size: 4090 813 275 178 EarI CTCTTCn'nnn_ Cuts at: 1353 3286 1353 Size: 1933 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2615 3943 4317 4463 387 Size: 1064 138 1026 1328 374 146 1280 Fragments arranged by size: 1328 1280 1064 1026 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2225 2411 3419 4016 4803 5071 Size: 393 470 123 186 1008 597 787 268 Cuts at: 5071 1239 Size: 1524 Fragments arranged by size: 1524 1008 787 597 470 393 268 186 123 EcoO109I rG'GnC_Cy Cuts at: 2451 2537 2451 Size: 86 5270 EcoRI G'AATT_C Cuts at: 2665 3043 2665 Size: 378 4978 FspI TGC'GCA Cuts at: 3862 3862 Size: 5356 HaeII r_GCGC'y Cuts at: 1678 2288 2425 2642 4503 1678 Size: 610 137 217 1861 2531 Fragments arranged by size: 2531 1861 610 217 137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3997 4029 4071 4103 1373 Size: 32 135 32 2425 32 42 32 2626 Fragments arranged by size: 2626 2425 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2700 2946 669 Size: 988 281 762 246 3079 Fragments arranged by size: 3079 988 762 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5356 HpaI GTT'AAC Cuts at: 2946 2946 Size: 5356 KasI G'GCGC_C Cuts at: 2638 2638 Size: 5356 KpnI G_GTAC'C Cuts at: 2681 2681 Size: 5356 MfeI C'AATT_G Cuts at: 2955 2955 Size: 5356 MluI A'CGCG_T Cuts at: 5232 5232 Size: 5356 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4470 4654 1305 Size: 3165 184 2007 Fragments arranged by size: 3165 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3534 Fragments arranged by size: 3534 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2408 3314 3673 3832 518 Size: 648 1242 906 359 159 2042 Fragments arranged by size: 2042 1242 906 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2170 2383 2461 2772 3449 4597 4842 1606 Size: 564 213 78 311 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 564 311 245 213 78 NaeI GCC'GGC Cuts at: 2490 2490 Size: 5356 NarI GG'CG_CC Cuts at: 2639 2639 Size: 5356 NcoI C'CATG_G Cuts at: 513 1065 2004 2073 2280 513 Size: 552 939 69 207 3589 Fragments arranged by size: 3589 939 552 207 69 NdeI CA'TA_TG Cuts at: 387 387 Size: 5356 NgoMIV G'CCGG_C Cuts at: 2488 2488 Size: 5356 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5356 NotI GC'GGCC_GC Cuts at: 2803 2803 Size: 5356 NspI r_CATG'y Cuts at: 1132 1229 2714 4259 1132 Size: 97 1485 1545 2229 Fragments arranged by size: 2229 1545 1485 97 PasI CC'CwG_GG Cuts at: 1348 2051 1348 Size: 703 4653 PciI A'CATG_T Cuts at: 4255 4255 Size: 5356 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5356 PmeI GTTT'AAAC Cuts at: 2660 2660 Size: 5356 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 4938 4970 3425 Size: 32 1481 32 3811 Fragments arranged by size: 3811 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5356 PsiI TTA'TAA Cuts at: 2926 2926 Size: 5356 PspOMI G'GGCC_C Cuts at: 2537 2537 Size: 5356 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2318 2350 2318 Size: 32 5324 PstI C_TGCA'G Cuts at: 830 2024 2228 2708 830 Size: 1194 204 480 3478 Fragments arranged by size: 3478 1194 480 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5356 PvuII CAG'CTG Cuts at: 2170 2170 Size: 5356 SalI G'TCGA_C Cuts at: 1936 2698 1936 Size: 762 4594 SbfI CC_TGCA'GG Cuts at: 2708 2708 Size: 5356 ScaI AGT'ACT Cuts at: 3604 5316 3604 Size: 1712 3644 SexAI A'CCwGG_T Cuts at: 2136 2136 Size: 5356 SfcI C'TryA_G Cuts at: 826 1045 2020 2224 2704 3839 4520 4711 826 Size: 219 975 204 480 1135 681 191 1471 Fragments arranged by size: 1471 1135 975 681 480 219 204 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2450 2450 Size: 5356 SfoI GGC'GCC Cuts at: 2640 2640 Size: 5356 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5356 SgrAI Cr'CCGG_yG Cuts at: 1700 2488 1700 Size: 788 4568 SmaI CCC'GGG Cuts at: 1872 2581 2683 1872 Size: 709 102 4545 Fragments arranged by size: 4545 709 102 SmlI C'TyrA_G Cuts at: 820 1017 1943 3460 4361 4623 820 Size: 197 926 1517 901 262 1553 Fragments arranged by size: 1553 1517 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3147 493 Size: 2654 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5356 SphI G_CATG'C Cuts at: 1132 2714 1132 Size: 1582 3774 SspI AAT'ATT Cuts at: 5 52 3280 5 Size: 47 3228 2081 Fragments arranged by size: 3228 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5356 StyI C'CwwG_G Cuts at: 513 1065 2004 2073 2100 2280 2520 2595 2795 Size: 552 939 69 27 180 240 75 200 Cuts at: 2795 513 Size: 3074 Fragments arranged by size: 3074 939 552 240 200 180 75 69 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2247 2558 3383 3400 3553 3738 4183 Size: 1255 667 311 825 17 153 185 445 Cuts at: 4183 325 Size: 1498 Fragments arranged by size: 1498 1255 825 667 445 311 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2310 2397 3602 Size: 274 80 33 51 1443 333 87 1205 Cuts at: 3602 5314 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1205 333 274 138 87 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5316 5348 5316 Size: 32 5324 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5356 XbaI T'CTAG_A Cuts at: 2692 2692 Size: 5356 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2287 2011 Size: 276 5080 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5356 XmaI C'CCGG_G Cuts at: 1870 2579 2681 1870 Size: 709 102 4545 Fragments arranged by size: 4545 709 102 XmnI GAAnn'nnTTC Cuts at: 3485 3485 Size: 5356 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3164 276 Size: 53 83 186 936 1630 2468 Fragments arranged by size: 2468 1630 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI RsrII SacI SacII SanDI SapI SrfI SwaI