(Circular) (Six-base) MAPSORT of: pFN21AB8844.seq Check: 3344 from: 1 to: 6271 pFN21AB8844 6271 bp With 182 enzymes: SgfI * April 15, 2010 09:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2856 3132 2856 Size: 276 5995 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3063 4081 278 Size: 53 83 186 936 1527 1018 2468 Fragments arranged by size: 2468 1527 1018 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3614 1937 Size: 1677 4594 Acc65I G'GTAC_C Cuts at: 3592 3592 Size: 6271 AclI AA'CG_TT Cuts at: 1563 4398 4771 1563 Size: 2835 373 3063 Fragments arranged by size: 3063 2835 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4334 5718 5986 1632 Size: 2702 1384 268 1917 Fragments arranged by size: 2702 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6074 AflIII A'CryG_T Cuts at: 1203 2967 3345 5170 6147 1203 Size: 1764 378 1825 977 1327 Fragments arranged by size: 1825 1764 1327 977 378 AgeI A'CCGG_T Cuts at: 5102 5102 Size: 6271 AhdI GACnn_n'nnGTC Cuts at: 5000 5000 Size: 6271 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6271 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6271 AlwNI CAG_nnn'CTG Cuts at: 2098 2338 2857 4014 5586 2098 Size: 240 519 1157 1572 2783 Fragments arranged by size: 2783 1572 1157 519 240 ApaI G_GGCC'C Cuts at: 3162 3162 Size: 6271 ApaLI G'TGCA_C Cuts at: 4328 5484 4328 Size: 1156 5115 ApoI r'AATT_y Cuts at: 1493 3580 3806 3958 6067 1493 Size: 2087 226 152 2109 1697 Fragments arranged by size: 2109 2087 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4825 160 Size: 872 3793 1606 Fragments arranged by size: 3793 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 AvaI C'yCGr_G Cuts at: 1870 1943 2056 2651 3596 1870 Size: 73 113 595 945 4545 Fragments arranged by size: 4545 945 595 113 73 BamHI G'GATC_C Cuts at: 2898 3601 3968 2898 Size: 703 367 5201 Fragments arranged by size: 5201 703 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2118 2182 3296 3592 5047 Size: 325 217 627 331 64 1114 296 1455 Cuts at: 5047 618 Size: 1842 Fragments arranged by size: 1842 1455 1114 627 331 325 296 217 64 BanII G_rGCy'C Cuts at: 1391 2823 2895 3111 3162 3505 3590 1391 Size: 1432 72 216 51 343 85 4072 Fragments arranged by size: 4072 1432 343 216 85 72 51 BbeI G_GCGC'C Cuts at: 2186 2186 Size: 6271 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5300 BbvCI CC'TCA_GC Cuts at: 2045 2045 Size: 6271 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2599 2633 2788 2822 4462 4496 1903 Size: 34 662 34 155 34 1640 34 3678 Fragments arranged by size: 3678 1640 662 155 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2576 4163 5373 2576 Size: 1587 1210 3474 Fragments arranged by size: 3474 1587 1210 BclI T'GATC_A Cuts at: 1548 3510 1548 Size: 1962 4309 BfrBI ATG'CAT Cuts at: 3436 3436 Size: 6271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2208 3173 3236 Size: 107 122 71 631 605 536 965 63 Cuts at: 3236 4882 136 Size: 1646 1525 Fragments arranged by size: 1646 1525 965 631 605 536 122 107 71 63 BglII A'GATC_T Cuts at: 6266 6266 Size: 6271 BlpI GC'TnA_GC Cuts at: 3688 3688 Size: 6271 Bme1580I G_kGCm'C Cuts at: 1790 2123 2535 2977 3162 3299 4332 5488 1790 Size: 333 412 442 185 137 1033 1156 2573 Fragments arranged by size: 2573 1156 1033 442 412 333 185 137 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6271 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2773 4002 4960 Size: 416 53 83 399 24 1356 1229 958 Cuts at: 4960 442 Size: 1753 Fragments arranged by size: 1753 1356 1229 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6271 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3544 3544 Size: 6271 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2798 4931 1239 Size: 1559 2133 2579 Fragments arranged by size: 2579 2133 1559 Bpu10I CC'TnA_GC Cuts at: 2045 2045 Size: 6271 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2549 2579 4396 5261 5559 2549 Size: 30 1817 865 298 3261 Fragments arranged by size: 3261 1817 865 298 30 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2030 2828 4934 882 Size: 617 531 798 2106 2219 Fragments arranged by size: 2219 2106 798 617 531 BsaAI yAC'GTr Cuts at: 493 1204 2280 3346 4062 493 Size: 711 1076 1066 716 2702 Fragments arranged by size: 2702 1076 1066 716 711 BsaBI GATnn'nnATC Cuts at: 2298 6265 2298 Size: 3967 2304 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2183 2325 3060 3069 Size: 53 83 186 936 650 142 735 9 Cuts at: 3069 4078 4460 5095 275 Size: 1009 382 635 1451 Fragments arranged by size: 1451 1009 936 735 650 635 382 186 142 83 53 9 BsaWI w'CCGG_w Cuts at: 4704 5102 5376 5523 5934 4704 Size: 398 274 147 411 5041 Fragments arranged by size: 5041 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2294 2324 3492 3522 1178 Size: 30 165 30 891 30 1168 30 3927 Fragments arranged by size: 3927 1168 891 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2062 1186 Size: 876 5395 BseYI C'CCAG_C Cuts at: 2077 2111 2206 3364 5474 5970 2077 Size: 34 95 1158 2110 496 2378 Fragments arranged by size: 2378 2110 1158 496 95 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2336 2630 2693 3293 1163 Size: 573 600 294 63 600 4141 Fragments arranged by size: 4141 600 600 573 294 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3135 3721 4482 5510 1540 Size: 275 186 1134 586 761 1028 2301 Fragments arranged by size: 2301 1134 1028 761 586 275 186 BsiHKAI G_wGCw'C Cuts at: 2658 3258 3342 4332 4417 5488 2658 Size: 600 84 990 85 1071 3441 Fragments arranged by size: 3441 1071 990 600 85 84 BsmI GAATG_Cn' Cuts at: 3566 3782 3875 3566 Size: 216 93 5962 Fragments arranged by size: 5962 216 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2123 2535 2658 2823 2895 2977 3111 Size: 399 333 412 123 165 72 82 134 Cuts at: 3111 3162 3258 3299 3342 3505 3590 4332 4417 Size: 51 96 41 43 163 85 742 85 Cuts at: 4417 5488 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 412 399 333 165 163 134 123 96 85 85 82 72 51 43 41 BspEI T'CCGG_A Cuts at: 5934 5934 Size: 6271 BspHI T'CATG_A Cuts at: 4160 5890 4160 Size: 1730 4541 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2427 2856 3132 3545 3626 844 Size: 1583 429 276 413 81 3489 Fragments arranged by size: 3489 1583 429 413 276 81 BsrBI CCG'CTC Cuts at: 1394 1823 4158 1394 Size: 429 2335 3507 Fragments arranged by size: 3507 2335 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4766 4940 65 Size: 1183 3518 174 1396 Fragments arranged by size: 3518 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2133 2249 2621 4915 5102 1700 Size: 110 323 116 372 2294 187 2869 Fragments arranged by size: 2869 2294 372 323 187 116 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6271 BssHII G'CGCG_C Cuts at: 1925 2245 2606 1925 Size: 320 361 5590 Fragments arranged by size: 5590 361 320 BssSI C'ACGA_G Cuts at: 2983 3529 4331 5343 2983 Size: 546 802 1012 3911 Fragments arranged by size: 3911 1012 802 546 BstAPI GCAn_nnn'nTGC Cuts at: 794 2857 794 Size: 2063 4208 BstBI TT'CG_AA Cuts at: 5075 5075 Size: 6271 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6271 BstYI r'GATC_y Cuts at: 1972 2898 3276 3601 3968 4353 4370 5822 5908 Size: 926 378 325 367 385 17 1452 86 Cuts at: 5908 5920 6266 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 926 385 378 367 346 325 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3374 3374 Size: 6271 BtgI C'CryG_G Cuts at: 513 1065 2004 2476 3234 3300 513 Size: 552 939 472 758 66 3484 Fragments arranged by size: 3484 939 758 552 472 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5532 Fragments arranged by size: 5532 683 56 BtsI GCAGTG_nn' Cuts at: 3032 3154 3787 4581 4601 3032 Size: 122 633 794 20 4702 Fragments arranged by size: 4702 794 633 122 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6271 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6236 DraI TTT'AAA Cuts at: 3575 3575 Size: 6271 DraIII CAC_nnn'GTG Cuts at: 3286 3286 Size: 6271 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5278 809 Size: 507 3962 1802 Fragments arranged by size: 3962 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 2739 2937 Size: 54 1425 50 275 18 421 488 198 Cuts at: 2937 3118 3132 3174 3718 4607 6236 8 Size: 181 14 42 544 889 1629 43 Fragments arranged by size: 1629 1425 889 544 488 421 275 198 181 54 50 43 42 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3132 3718 1537 Size: 275 1320 586 4090 Fragments arranged by size: 4090 1320 586 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2477 3246 4201 1353 Size: 1124 769 955 3423 Fragments arranged by size: 3423 1124 955 769 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4858 5232 5378 387 Size: 1064 138 3269 374 146 1280 Fragments arranged by size: 3269 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2798 4334 4931 5718 5986 1239 Size: 393 1166 1536 597 787 268 1524 Fragments arranged by size: 1536 1524 1166 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2177 3092 2177 Size: 915 5356 EcoO109I rG'GnC_Cy Cuts at: 2052 2082 3158 3159 2052 Size: 30 1076 1 5164 Fragments arranged by size: 5164 1076 30 1 EcoRI G'AATT_C Cuts at: 3580 3958 3580 Size: 378 5893 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2695 2695 Size: 6271 FspI TGC'GCA Cuts at: 2423 2540 4777 2423 Size: 117 2237 3917 Fragments arranged by size: 3917 2237 117 FspAI rTGC'GCAy Cuts at: 2540 2540 Size: 6271 HaeII r_GCGC'y Cuts at: 1678 2168 2186 2459 3010 5418 1678 Size: 490 18 273 551 2408 2531 Fragments arranged by size: 2531 2408 551 490 273 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3421 3453 4912 4944 4986 Size: 32 135 32 1849 32 1459 32 42 Cuts at: 4986 5018 1373 Size: 32 2626 Fragments arranged by size: 2626 1849 1459 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3615 3861 669 Size: 988 281 1677 246 3079 Fragments arranged by size: 3079 1677 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6271 HpaI GTT'AAC Cuts at: 3861 3861 Size: 6271 KasI G'GCGC_C Cuts at: 2182 2182 Size: 6271 KpnI G_GTAC'C Cuts at: 3596 3596 Size: 6271 MfeI C'AATT_G Cuts at: 3870 3870 Size: 6271 MluI A'CGCG_T Cuts at: 6147 6147 Size: 6271 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2394 2895 5385 5569 1305 Size: 1089 501 2490 184 2007 Fragments arranged by size: 2490 2007 1089 501 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2741 10 Size: 54 1425 343 909 3540 Fragments arranged by size: 3540 1425 909 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4229 4588 4747 518 Size: 648 3063 359 159 2042 Fragments arranged by size: 3063 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2115 2128 2863 3117 3687 4364 5512 5757 Size: 509 13 735 254 570 677 1148 245 Cuts at: 5757 1606 Size: 2120 Fragments arranged by size: 2120 1148 735 677 570 509 254 245 13 NaeI GCC'GGC Cuts at: 2251 2623 2251 Size: 372 5899 NarI GG'CG_CC Cuts at: 2183 2183 Size: 6271 NcoI C'CATG_G Cuts at: 513 1065 2004 3300 513 Size: 552 939 1296 3484 Fragments arranged by size: 3484 1296 939 552 NdeI CA'TA_TG Cuts at: 387 2505 387 Size: 2118 4153 NgoMIV G'CCGG_C Cuts at: 2249 2621 2249 Size: 372 5899 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6271 NotI GC'GGCC_GC Cuts at: 3718 3718 Size: 6271 NsiI A_TGCA'T Cuts at: 3438 3438 Size: 6271 NspI r_CATG'y Cuts at: 1132 1229 3629 5174 1132 Size: 97 2400 1545 2229 Fragments arranged by size: 2400 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6271 PciI A'CATG_T Cuts at: 5170 5170 Size: 6271 PflMI CCAn_nnn'nTGG Cuts at: 1108 3286 1108 Size: 2178 4093 PfoI T'CCnGG_A Cuts at: 2547 3063 3350 2547 Size: 516 287 5468 Fragments arranged by size: 5468 516 287 PmeI GTTT'AAAC Cuts at: 3575 3575 Size: 6271 PmlI CAC'GTG Cuts at: 2280 2280 Size: 6271 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4340 4372 5853 5885 4340 Size: 32 1481 32 4726 Fragments arranged by size: 4726 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6271 PsiI TTA'TAA Cuts at: 3841 3841 Size: 6271 PspOMI G'GGCC_C Cuts at: 3158 3158 Size: 6271 PspXI vC'TCGA_Gb Cuts at: 2651 2651 Size: 6271 PstI C_TGCA'G Cuts at: 830 2454 2853 2862 3623 830 Size: 1624 399 9 761 3478 Fragments arranged by size: 3478 1624 761 399 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6271 PvuII CAG'CTG Cuts at: 2128 2863 2128 Size: 735 5536 SalI G'TCGA_C Cuts at: 1936 3613 1936 Size: 1677 4594 SapI GCTCTTCn'nnn_ Cuts at: 3246 3246 Size: 6271 SbfI CC_TGCA'GG Cuts at: 3623 3623 Size: 6271 ScaI AGT'ACT Cuts at: 4519 6231 4519 Size: 1712 4559 SfcI C'TryA_G Cuts at: 826 1045 2450 2849 2858 3619 4754 5435 5626 Size: 219 1405 399 9 761 1135 681 191 Cuts at: 5626 826 Size: 1471 Fragments arranged by size: 1471 1405 1135 761 681 399 219 191 9 SfoI GGC'GCC Cuts at: 2184 2184 Size: 6271 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6271 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6271 SmaI CCC'GGG Cuts at: 1872 3598 1872 Size: 1726 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2564 2594 2651 4375 5276 5538 Size: 197 926 621 30 57 1724 901 262 Cuts at: 5538 820 Size: 1553 Fragments arranged by size: 1724 1553 926 901 621 262 197 57 30 SnaBI TAC'GTA Cuts at: 493 4062 493 Size: 3569 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6271 SphI G_CATG'C Cuts at: 1132 3629 1132 Size: 2497 3774 SspI AAT'ATT Cuts at: 5 52 4195 5 Size: 47 4143 2081 Fragments arranged by size: 4143 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6271 StyI C'CwwG_G Cuts at: 513 1065 2004 3300 3710 513 Size: 552 939 1296 410 3074 Fragments arranged by size: 3074 1296 939 552 410 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2012 2396 4298 4315 4468 4653 5098 Size: 1255 432 384 1902 17 153 185 445 Cuts at: 5098 325 Size: 1498 Fragments arranged by size: 1902 1498 1255 445 432 384 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2154 2811 4517 Size: 274 80 33 51 1443 177 657 1706 Cuts at: 4517 6229 96 Size: 1712 138 Fragments arranged by size: 1712 1706 1443 657 274 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2294 2326 2837 2869 3492 3524 6231 Size: 32 82 32 511 32 623 32 2707 Cuts at: 6231 6263 2180 Size: 32 2188 Fragments arranged by size: 2707 2188 623 511 82 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2666 1316 Size: 1350 4921 XbaI T'CTAG_A Cuts at: 3607 3607 Size: 6271 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3439 3439 Size: 6271 XhoI C'TCGA_G Cuts at: 1943 2651 1943 Size: 708 5563 XmaI C'CCGG_G Cuts at: 1870 3596 1870 Size: 1726 4545 XmnI GAAnn'nnTTC Cuts at: 3359 4400 3359 Size: 1041 5230 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3061 4079 276 Size: 53 83 186 936 1527 1018 2468 Fragments arranged by size: 2468 1527 1018 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FseI NruI PacI PpuMI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI