Restriction map for
pFN21AB8844
(Linear) (Six-base) MAPSORT of: KIBB8844.seq Check: 4878 from: 1 to: 1566
KIBB8844.orf
With 182 enzymes: SgfI *
March 2, 2010 14:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 851 1127 1566
Size: 851 276 439
Fragments arranged by size:
851 439 276
AatII G_ACGT'C
Cuts at: 0 1058 1566
Size: 1058 508
AflIII A'CryG_T
Cuts at: 0 962 1340 1566
Size: 962 378 226
Fragments arranged by size:
962 378 226
AlwNI CAG_nnn'CTG
Cuts at: 0 93 333 852 1566
Size: 93 240 519 714
Fragments arranged by size:
714 519 240 93
ApaI G_GGCC'C
Cuts at: 0 1157 1566
Size: 1157 409
AvaI C'yCGr_G
Cuts at: 0 51 646 1566
Size: 51 595 920
Fragments arranged by size:
920 595 51
BamHI G'GATC_C
Cuts at: 0 893 1566
Size: 893 673
BanI G'GyrC_C
Cuts at: 0 113 177 1291 1566
Size: 113 64 1114 275
Fragments arranged by size:
1114 275 113 64
BanII G_rGCy'C
Cuts at: 0 818 890 1106 1157 1500 1566
Size: 818 72 216 51 343 66
Fragments arranged by size:
818 343 216 72 66 51
BbeI G_GCGC'C
Cuts at: 0 181 1566
Size: 181 1385
BbvCI CC'TCA_GC
Cuts at: 0 40 1566
Size: 40 1526
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 594 628 783 817 1566
Size: 594 34 155 34 749
Fragments arranged by size:
749 594 155 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 571 1566
Size: 571 995
BclI T'GATC_A
Cuts at: 0 1505 1566
Size: 1505 61
BfrBI ATG'CAT
Cuts at: 0 1431 1566
Size: 1431 135
BglI GCCn_nnn'nGGC
Cuts at: 0 203 1168 1231 1566
Size: 203 965 63 335
Fragments arranged by size:
965 335 203 63
Bme1580I G_kGCm'C
Cuts at: 0 118 530 972 1157 1294 1566
Size: 118 412 442 185 137 272
Fragments arranged by size:
442 412 272 185 137 118
BmrI ACTGGGnnnn_n'
Cuts at: 0 768 1566
Size: 768 798
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1539 1566
Size: 1539 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1566
Size: 793 773
Bpu10I CC'TnA_GC
Cuts at: 0 40 1566
Size: 40 1526
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 544 574 1566
Size: 544 30 992
Fragments arranged by size:
992 544 30
BsaI GGTCTCn'nnnn_
Cuts at: 0 25 823 1566
Size: 25 798 743
Fragments arranged by size:
798 743 25
BsaAI yAC'GTr
Cuts at: 0 275 1341 1566
Size: 275 1066 225
Fragments arranged by size:
1066 275 225
BsaBI GATnn'nnATC
Cuts at: 0 293 1566
Size: 293 1273
BsaHI Gr'CG_yC
Cuts at: 0 178 320 1055 1064 1566
Size: 178 142 735 9 502
Fragments arranged by size:
735 502 178 142 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 1487 1517 1566
Size: 289 30 1168 30 49
Fragments arranged by size:
1168 289 49 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 57 1566
Size: 57 1509
BseYI C'CCAG_C
Cuts at: 0 72 106 201 1359 1566
Size: 72 34 95 1158 207
Fragments arranged by size:
1158 207 95 72 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 625 688 1288 1566
Size: 331 294 63 600 278
Fragments arranged by size:
600 331 294 278 63
BsiEI CG_ry'CG
Cuts at: 0 1130 1566
Size: 1130 436
BsiHKAI G_wGCw'C
Cuts at: 0 653 1253 1337 1566
Size: 653 600 84 229
Fragments arranged by size:
653 600 229 84
BsmI GAATG_Cn'
Cuts at: 0 1561 1566
Size: 1561 5
Bsp1286I G_dGCh'C
Cuts at: 0 118 530 653 818 890 972 1106 1157
Size: 118 412 123 165 72 82 134 51
Cuts at: 1157 1253 1294 1337 1500 1566
Size: 96 41 43 163 66
Fragments arranged by size:
412 165 163 134 123 118 96 82
72 66 51 43 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 422 851 1127 1540 1566
Size: 422 429 276 413 26
Fragments arranged by size:
429 422 413 276 26
BsrFI r'CCGG_y
Cuts at: 0 128 244 616 1566
Size: 128 116 372 950
Fragments arranged by size:
950 372 128 116
BssHII G'CGCG_C
Cuts at: 0 240 601 1566
Size: 240 361 965
Fragments arranged by size:
965 361 240
BssSI C'ACGA_G
Cuts at: 0 978 1524 1566
Size: 978 546 42
Fragments arranged by size:
978 546 42
BstAPI GCAn_nnn'nTGC
Cuts at: 0 852 1566
Size: 852 714
BstYI r'GATC_y
Cuts at: 0 893 1271 1566
Size: 893 378 295
Fragments arranged by size:
893 378 295
Bsu36I CC'TnA_GG
Cuts at: 0 1369 1566
Size: 1369 197
BtgI C'CryG_G
Cuts at: 0 471 1229 1295 1566
Size: 471 758 66 271
Fragments arranged by size:
758 471 271 66
BtsI GCAGTG_nn'
Cuts at: 0 1027 1149 1566
Size: 1027 122 417
Fragments arranged by size:
1027 417 122
DraIII CAC_nnn'GTG
Cuts at: 0 1281 1566
Size: 1281 285
EaeI y'GGCC_r
Cuts at: 0 246 734 932 1113 1127 1169 1566
Size: 246 488 198 181 14 42 397
Fragments arranged by size:
488 397 246 198 181 42 14
EagI C'GGCC_G
Cuts at: 0 1127 1566
Size: 1127 439
EarI CTCTTCn'nnn_
Cuts at: 0 472 1241 1566
Size: 472 769 325
Fragments arranged by size:
769 472 325
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 793 1566
Size: 793 773
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 1087 1566
Size: 172 915 479
Fragments arranged by size:
915 479 172
EcoO109I rG'GnC_Cy
Cuts at: 0 47 77 1153 1154 1566
Size: 47 30 1076 1 412
Fragments arranged by size:
1076 412 47 30 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 690 1566
Size: 690 876
FspI TGC'GCA
Cuts at: 0 418 535 1566
Size: 418 117 1031
Fragments arranged by size:
1031 418 117
FspAI rTGC'GCAy
Cuts at: 0 535 1566
Size: 535 1031
HaeII r_GCGC'y
Cuts at: 0 163 181 454 1005 1566
Size: 163 18 273 551 561
Fragments arranged by size:
561 551 273 163 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1416 1448 1566
Size: 1416 32 118
Fragments arranged by size:
1416 118 32
KasI G'GCGC_C
Cuts at: 0 177 1566
Size: 177 1389
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 890 1566
Size: 389 501 676
Fragments arranged by size:
676 501 389
MscI TGG'CCA
Cuts at: 0 736 1566
Size: 736 830
MspA1I CmG'CkG
Cuts at: 0 110 123 858 1112 1566
Size: 110 13 735 254 454
Fragments arranged by size:
735 454 254 110 13
NaeI GCC'GGC
Cuts at: 0 246 618 1566
Size: 246 372 948
Fragments arranged by size:
948 372 246
NarI GG'CG_CC
Cuts at: 0 178 1566
Size: 178 1388
NcoI C'CATG_G
Cuts at: 0 1295 1566
Size: 1295 271
NdeI CA'TA_TG
Cuts at: 0 500 1566
Size: 500 1066
NgoMIV G'CCGG_C
Cuts at: 0 244 616 1566
Size: 244 372 950
Fragments arranged by size:
950 372 244
NsiI A_TGCA'T
Cuts at: 0 1433 1566
Size: 1433 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 1281 1566
Size: 1281 285
PfoI T'CCnGG_A
Cuts at: 0 542 1058 1345 1566
Size: 542 516 287 221
Fragments arranged by size:
542 516 287 221
PmlI CAC'GTG
Cuts at: 0 275 1566
Size: 275 1291
PspOMI G'GGCC_C
Cuts at: 0 1153 1566
Size: 1153 413
PspXI vC'TCGA_Gb
Cuts at: 0 646 1566
Size: 646 920
PstI C_TGCA'G
Cuts at: 0 449 848 857 1566
Size: 449 399 9 709
Fragments arranged by size:
709 449 399 9
PvuII CAG'CTG
Cuts at: 0 123 858 1566
Size: 123 735 708
Fragments arranged by size:
735 708 123
SapI GCTCTTCn'nnn_
Cuts at: 0 1241 1566
Size: 1241 325
SfcI C'TryA_G
Cuts at: 0 445 844 853 1566
Size: 445 399 9 713
Fragments arranged by size:
713 445 399 9
SfoI GGC'GCC
Cuts at: 0 179 1566
Size: 179 1387
SmlI C'TyrA_G
Cuts at: 0 559 589 646 1566
Size: 559 30 57 920
Fragments arranged by size:
920 559 57 30
StyI C'CwwG_G
Cuts at: 0 1295 1566
Size: 1295 271
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 7 391 1566
Size: 7 384 1175
Fragments arranged by size:
1175 384 7
TatI w'GTAC_w
Cuts at: 0 149 806 1566
Size: 149 657 760
Fragments arranged by size:
760 657 149
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 289 321 832 864 1487 1519
Size: 175 32 82 32 511 32 623 32
Cuts at: 1519 1566
Size: 47
Fragments arranged by size:
623 511 175 82 47 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 661 1566
Size: 661 905
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1434 1566
Size: 1434 132
XhoI C'TCGA_G
Cuts at: 0 646 1566
Size: 646 920
XmnI GAAnn'nnTTC
Cuts at: 0 1354 1566
Size: 1354 212
ZraI GAC'GTC
Cuts at: 0 1056 1566
Size: 1056 510
Enzymes that do cut:
AarI AatII AflIII AlwNI ApaI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstYI
Bsu36I BtgI BtsI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I KasI
MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI PflMI PfoI PmlI PspOMI PspXI PstI PvuII
SapI SfcI SfoI SmlI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BglII BlpI BmgBI BmtI BsaWI
BsiWI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI
EciI EcoICRI EcoRI EcoRV FseI HincII HindIII HpaI
KpnI MfeI MluI MslI NheI NotI NruI NspI
PacI PasI PciI PmeI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XmaI