(Circular) (Six-base) MAPSORT of: pFN21AB6967.seq Check: 4676 from: 1 to: 5350 pFN21AB6967 5350 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2587 3160 278 Size: 53 83 186 936 1051 573 2468 Fragments arranged by size: 2468 1051 936 573 186 83 53 AccI GT'mk_AC Cuts at: 1937 2693 1937 Size: 756 4594 Acc65I G'GTAC_C Cuts at: 2671 2671 Size: 5350 AclI AA'CG_TT Cuts at: 1563 3477 3850 1563 Size: 1914 373 3063 Fragments arranged by size: 3063 1914 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3413 4797 5065 1632 Size: 1781 1384 268 1917 Fragments arranged by size: 1917 1781 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5153 AflIII A'CryG_T Cuts at: 1203 4249 5226 1203 Size: 3046 977 1327 Fragments arranged by size: 3046 1327 977 AgeI A'CCGG_T Cuts at: 4181 4181 Size: 5350 AhdI GACnn_n'nnGTC Cuts at: 4079 4079 Size: 5350 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5350 AlwNI CAG_nnn'CTG Cuts at: 2075 3093 4665 2075 Size: 1018 1572 2760 Fragments arranged by size: 2760 1572 1018 ApaLI G'TGCA_C Cuts at: 3407 4563 3407 Size: 1156 4194 ApoI r'AATT_y Cuts at: 1493 2388 2659 2885 3037 5146 1493 Size: 895 271 226 152 2109 1697 Fragments arranged by size: 2109 1697 895 271 226 152 AseI AT'TA_AT Cuts at: 160 1032 3904 160 Size: 872 2872 1606 Fragments arranged by size: 2872 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 AvaI C'yCGr_G Cuts at: 1870 1943 2021 2675 1870 Size: 73 78 654 4545 Fragments arranged by size: 4545 654 78 73 BamHI G'GATC_C Cuts at: 2680 3047 2680 Size: 367 4983 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2045 2671 4126 618 Size: 325 217 627 258 626 1455 1842 Fragments arranged by size: 1842 1455 627 626 325 258 217 BanII G_rGCy'C Cuts at: 1391 2271 2290 2669 1391 Size: 880 19 379 4072 Fragments arranged by size: 4072 880 379 19 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2099 928 Size: 971 200 4179 Fragments arranged by size: 4179 971 200 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3541 3575 1903 Size: 34 50 34 1520 34 3678 Fragments arranged by size: 3678 1520 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3242 4452 3242 Size: 1210 4140 BclI T'GATC_A Cuts at: 1548 1548 Size: 5350 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3961 136 Size: 107 122 71 631 605 337 1952 1525 Fragments arranged by size: 1952 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5345 5345 Size: 5350 BlpI GC'TnA_GC Cuts at: 2767 2767 Size: 5350 Bme1580I G_kGCm'C Cuts at: 1790 2048 2069 3411 4567 1790 Size: 258 21 1342 1156 2573 Fragments arranged by size: 2573 1342 1156 258 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5350 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2145 3081 4039 Size: 416 53 83 399 24 728 936 958 Cuts at: 4039 442 Size: 1753 Fragments arranged by size: 1753 958 936 728 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5350 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2472 2472 Size: 5350 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4010 1239 Size: 2771 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3475 4340 4638 3475 Size: 865 298 4187 Fragments arranged by size: 4187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4013 882 Size: 617 2514 2219 Fragments arranged by size: 2514 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3141 493 Size: 711 1937 2702 Fragments arranged by size: 2702 1937 711 BsaBI GATnn'nnATC Cuts at: 2476 2568 5344 2476 Size: 92 2776 2482 Fragments arranged by size: 2776 2482 92 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2584 3157 3539 4174 Size: 53 83 186 936 1051 573 382 635 Cuts at: 4174 275 Size: 1451 Fragments arranged by size: 1451 1051 936 635 573 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2229 3783 4181 4455 4602 5013 2229 Size: 1554 398 274 147 411 2566 Fragments arranged by size: 2566 1554 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5125 Fragments arranged by size: 5125 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2484 1186 Size: 1298 4052 BseYI C'CCAG_C Cuts at: 2075 4553 5049 2075 Size: 2478 496 2376 Fragments arranged by size: 2478 2376 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2649 1163 Size: 573 913 3864 Fragments arranged by size: 3864 913 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2800 3561 4589 1540 Size: 275 186 799 761 1028 2301 Fragments arranged by size: 2301 1028 799 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2271 2290 3411 3496 4567 2271 Size: 19 1121 85 1071 3054 Fragments arranged by size: 3054 1121 1071 85 19 BsmI GAATG_Cn' Cuts at: 2861 2954 2861 Size: 93 5257 BsmBI CGTCTCn'nnnn_ Cuts at: 2575 2629 2575 Size: 54 5296 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2069 2271 2290 2669 3411 3496 Size: 399 258 21 202 19 379 742 85 Cuts at: 3496 4567 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 379 258 202 85 21 19 BspEI T'CCGG_A Cuts at: 2229 5013 2229 Size: 2784 2566 BspHI T'CATG_A Cuts at: 3239 4969 3239 Size: 1730 3620 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2003 2383 2705 844 Size: 1159 380 322 3489 Fragments arranged by size: 3489 1159 380 322 BsrBI CCG'CTC Cuts at: 1394 1823 3237 1394 Size: 429 1414 3507 Fragments arranged by size: 3507 1414 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3845 4019 65 Size: 1183 2597 174 1396 Fragments arranged by size: 2597 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3994 4181 1700 Size: 110 2184 187 2869 Fragments arranged by size: 2869 2184 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5350 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5350 BssSI C'ACGA_G Cuts at: 2533 3410 4422 2533 Size: 877 1012 3461 Fragments arranged by size: 3461 1012 877 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5350 BstBI TT'CG_AA Cuts at: 4154 4154 Size: 5350 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5350 BstXI CCAn_nnnn'nTGG Cuts at: 2186 2461 2186 Size: 275 5075 BstYI r'GATC_y Cuts at: 1972 2680 3047 3432 3449 4901 4987 4999 5345 Size: 708 367 385 17 1452 86 12 346 Cuts at: 5345 1972 Size: 1977 Fragments arranged by size: 1977 1452 708 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4798 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4611 Fragments arranged by size: 4611 683 56 BtsI GCAGTG_nn' Cuts at: 2866 3660 3680 2866 Size: 794 20 4536 Fragments arranged by size: 4536 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5350 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5315 DraI TTT'AAA Cuts at: 2514 2654 2514 Size: 140 5210 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4357 809 Size: 507 3041 1802 Fragments arranged by size: 3041 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2434 2797 3686 Size: 54 1425 50 275 18 604 363 889 Cuts at: 3686 5315 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 604 363 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2797 1537 Size: 275 985 4090 Fragments arranged by size: 4090 985 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2406 2590 3280 1353 Size: 1053 184 690 3423 Fragments arranged by size: 3423 1053 690 184 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3937 4311 4457 387 Size: 1064 138 2348 374 146 1280 Fragments arranged by size: 2348 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2269 2288 2269 Size: 19 5331 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3413 4010 4797 5065 1239 Size: 393 1781 597 787 268 1524 Fragments arranged by size: 1781 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2032 2059 2032 Size: 27 5323 EcoRI G'AATT_C Cuts at: 2659 3037 2659 Size: 378 4972 FspI TGC'GCA Cuts at: 3856 3856 Size: 5350 HaeII r_GCGC'y Cuts at: 1678 4497 1678 Size: 2819 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3991 4023 4065 4097 1373 Size: 32 135 32 2419 32 42 32 2626 Fragments arranged by size: 2626 2419 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2018 2694 2940 669 Size: 988 281 80 676 246 3079 Fragments arranged by size: 3079 988 676 281 246 80 HindIII A'AGCT_T Cuts at: 748 748 Size: 5350 HpaI GTT'AAC Cuts at: 2940 2940 Size: 5350 KpnI G_GTAC'C Cuts at: 2675 2675 Size: 5350 MfeI C'AATT_G Cuts at: 2949 2949 Size: 5350 MluI A'CGCG_T Cuts at: 5226 5226 Size: 5350 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4464 4648 1305 Size: 3159 184 2007 Fragments arranged by size: 3159 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2436 10 Size: 54 1425 343 604 2924 Fragments arranged by size: 2924 1425 604 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2241 3308 3667 3826 518 Size: 648 1075 1067 359 159 2042 Fragments arranged by size: 2042 1075 1067 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2099 2296 2302 2405 2766 3443 4591 4836 Size: 493 197 6 103 361 677 1148 245 Cuts at: 4836 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 493 361 245 197 103 6 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4798 NdeI CA'TA_TG Cuts at: 387 387 Size: 5350 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5350 NotI GC'GGCC_GC Cuts at: 2797 2797 Size: 5350 NspI r_CATG'y Cuts at: 1132 1229 2708 4253 1132 Size: 97 1479 1545 2229 Fragments arranged by size: 2229 1545 1479 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5350 PciI A'CATG_T Cuts at: 4249 4249 Size: 5350 PflMI CCAn_nnn'nTGG Cuts at: 1108 2075 1108 Size: 967 4383 PfoI T'CCnGG_A Cuts at: 2576 2576 Size: 5350 PmeI GTTT'AAAC Cuts at: 2654 2654 Size: 5350 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3419 3451 4932 4964 3419 Size: 32 1481 32 3805 Fragments arranged by size: 3805 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2059 2032 Size: 27 5323 PshAI GACnn'nnGTC Cuts at: 1613 2197 1613 Size: 584 4766 PsiI TTA'TAA Cuts at: 2920 2920 Size: 5350 PspXI vC'TCGA_Gb Cuts at: 2021 2021 Size: 5350 PstI C_TGCA'G Cuts at: 830 2295 2301 2329 2702 830 Size: 1465 6 28 373 3478 Fragments arranged by size: 3478 1465 373 28 6 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5350 PvuII CAG'CTG Cuts at: 2099 2296 2405 2099 Size: 197 109 5044 Fragments arranged by size: 5044 197 109 SacI G_AGCT'C Cuts at: 2271 2290 2271 Size: 19 5331 SalI G'TCGA_C Cuts at: 1936 2692 1936 Size: 756 4594 SanDI GG'GwC_CC Cuts at: 2032 2032 Size: 5350 SapI GCTCTTCn'nnn_ Cuts at: 2590 2590 Size: 5350 SbfI CC_TGCA'GG Cuts at: 2702 2702 Size: 5350 ScaI AGT'ACT Cuts at: 3598 5310 3598 Size: 1712 3638 SfcI C'TryA_G Cuts at: 826 1045 2291 2297 2325 2698 3833 4514 4705 Size: 219 1246 6 28 373 1135 681 191 Cuts at: 4705 826 Size: 1471 Fragments arranged by size: 1471 1246 1135 681 373 219 191 28 6 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5350 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5350 SmaI CCC'GGG Cuts at: 1872 2677 1872 Size: 805 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2021 3454 4355 4617 820 Size: 197 926 78 1433 901 262 1553 Fragments arranged by size: 1553 1433 926 901 262 197 78 SnaBI TAC'GTA Cuts at: 493 3141 493 Size: 2648 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5350 SphI G_CATG'C Cuts at: 1132 2708 1132 Size: 1576 3774 SspI AAT'ATT Cuts at: 5 52 3274 5 Size: 47 3222 2081 Fragments arranged by size: 3222 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5350 StyI C'CwwG_G Cuts at: 513 1065 2789 513 Size: 552 1724 3074 Fragments arranged by size: 3074 1724 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2063 2067 3377 3394 3547 3732 4177 Size: 1255 483 4 1310 17 153 185 445 Cuts at: 4177 325 Size: 1498 Fragments arranged by size: 1498 1310 1255 483 445 185 153 17 4 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3596 5308 96 Size: 274 80 33 51 1443 1619 1712 138 Fragments arranged by size: 1712 1619 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5310 5342 5310 Size: 32 5318 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5350 XbaI T'CTAG_A Cuts at: 2686 2686 Size: 5350 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2183 2183 Size: 5350 XhoI C'TCGA_G Cuts at: 1943 2021 1943 Size: 78 5272 XmaI C'CCGG_G Cuts at: 1870 2675 1870 Size: 805 4545 XmnI GAAnn'nnTTC Cuts at: 2389 3479 2389 Size: 1090 4260 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2585 3158 276 Size: 53 83 186 936 1051 573 2468 Fragments arranged by size: 2468 1051 936 573 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI