Restriction map for
pFN21AB6967
(Linear) (Six-base) MAPSORT of: KIBB6967.seq Check: 2368 from: 1 to: 645
KIBB6967.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AatII G_ACGT'C
Cuts at: 0 582 645
Size: 582 63
AlwNI CAG_nnn'CTG
Cuts at: 0 70 645
Size: 70 575
ApoI r'AATT_y
Cuts at: 0 383 645
Size: 383 262
AvaI C'yCGr_G
Cuts at: 0 16 645
Size: 16 629
BanI G'GyrC_C
Cuts at: 0 40 645
Size: 40 605
BanII G_rGCy'C
Cuts at: 0 266 285 645
Size: 266 19 360
Fragments arranged by size:
360 266 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 94 645
Size: 94 551
Bme1580I G_kGCm'C
Cuts at: 0 43 64 645
Size: 43 21 581
Fragments arranged by size:
581 43 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 140 645
Size: 140 505
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 467 645
Size: 467 178
BsaBI GATnn'nnATC
Cuts at: 0 471 563 645
Size: 471 92 82
Fragments arranged by size:
471 92 82
BsaHI Gr'CG_yC
Cuts at: 0 579 645
Size: 579 66
BsaWI w'CCGG_w
Cuts at: 0 224 645
Size: 224 421
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 479 645
Size: 479 166
BseYI C'CCAG_C
Cuts at: 0 70 645
Size: 70 575
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 644 645
Size: 644 1
BsiHKAI G_wGCw'C
Cuts at: 0 266 285 645
Size: 266 19 360
Fragments arranged by size:
360 266 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 570 624 645
Size: 570 54 21
Fragments arranged by size:
570 54 21
Bsp1286I G_dGCh'C
Cuts at: 0 43 64 266 285 645
Size: 43 21 202 19 360
Fragments arranged by size:
360 202 43 21 19
BspEI T'CCGG_A
Cuts at: 0 224 645
Size: 224 421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 378 645
Size: 378 267
BssSI C'ACGA_G
Cuts at: 0 528 645
Size: 528 117
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 456 645
Size: 181 275 189
Fragments arranged by size:
275 189 181
DraI TTT'AAA
Cuts at: 0 509 645
Size: 509 136
EaeI y'GGCC_r
Cuts at: 0 429 645
Size: 429 216
EarI CTCTTCn'nnn_
Cuts at: 0 401 585 645
Size: 401 184 60
Fragments arranged by size:
401 184 60
EcoICRI GAG'CTC
Cuts at: 0 264 283 645
Size: 264 19 362
Fragments arranged by size:
362 264 19
EcoO109I rG'GnC_Cy
Cuts at: 0 27 54 645
Size: 27 27 591
Fragments arranged by size:
591 27 27
HincII GTy'rAC
Cuts at: 0 13 645
Size: 13 632
MscI TGG'CCA
Cuts at: 0 431 645
Size: 431 214
MslI CAynn'nnrTG
Cuts at: 0 236 645
Size: 236 409
MspA1I CmG'CkG
Cuts at: 0 94 291 297 400 645
Size: 94 197 6 103 245
Fragments arranged by size:
245 197 103 94 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 70 645
Size: 70 575
PfoI T'CCnGG_A
Cuts at: 0 571 645
Size: 571 74
PpuMI rG'GwC_Cy
Cuts at: 0 27 54 645
Size: 27 27 591
Fragments arranged by size:
591 27 27
PshAI GACnn'nnGTC
Cuts at: 0 192 645
Size: 192 453
PspXI vC'TCGA_Gb
Cuts at: 0 16 645
Size: 16 629
PstI C_TGCA'G
Cuts at: 0 290 296 324 645
Size: 290 6 28 321
Fragments arranged by size:
321 290 28 6
PvuII CAG'CTG
Cuts at: 0 94 291 400 645
Size: 94 197 109 245
Fragments arranged by size:
245 197 109 94
SacI G_AGCT'C
Cuts at: 0 266 285 645
Size: 266 19 360
Fragments arranged by size:
360 266 19
SanDI GG'GwC_CC
Cuts at: 0 27 645
Size: 27 618
SapI GCTCTTCn'nnn_
Cuts at: 0 585 645
Size: 585 60
SfcI C'TryA_G
Cuts at: 0 286 292 320 645
Size: 286 6 28 325
Fragments arranged by size:
325 286 28 6
SmlI C'TyrA_G
Cuts at: 0 16 645
Size: 16 629
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 58 62 645
Size: 58 4 583
Fragments arranged by size:
583 58 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 178 645
Size: 178 467
XhoI C'TCGA_G
Cuts at: 0 16 645
Size: 16 629
XmnI GAAnn'nnTTC
Cuts at: 0 384 645
Size: 384 261
ZraI GAC'GTC
Cuts at: 0 580 645
Size: 580 65
Enzymes that do cut:
AatII AlwNI ApoI AvaI BanI BanII BbsI Bme1580I
BmrI BplI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BssSI BstXI DraI
EaeI EarI EcoICRI EcoO109I HincII MscI MslI MspA1I
PflMI PfoI PpuMI PshAI PspXI PstI PvuII SacI
SanDI SapI SfcI SmlI TaqII XcmI XhoI XmnI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BsiEI BsiWI
BsmI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TatI TstI
Tth111I XbaI XmaI