(Circular) (Six-base) MAPSORT of: pFN21AB6966.seq Check: 5195 from: 1 to: 5428 pFN21AB6966 5428 bp With 182 enzymes: SgfI * October 20, 2009 10:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3238 278 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2771 1937 Size: 834 4594 Acc65I G'GTAC_C Cuts at: 2280 2749 2280 Size: 469 4959 AclI AA'CG_TT Cuts at: 1563 3555 3928 1563 Size: 1992 373 3063 Fragments arranged by size: 3063 1992 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2238 2435 2496 3491 4875 5143 1632 Size: 399 207 197 61 995 1384 268 1917 Fragments arranged by size: 1917 1384 995 399 268 207 197 61 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5231 AflIII A'CryG_T Cuts at: 1203 4327 5304 1203 Size: 3124 977 1327 Fragments arranged by size: 3124 1327 977 AgeI A'CCGG_T Cuts at: 4259 4259 Size: 5428 AhdI GACnn_n'nnGTC Cuts at: 4157 4157 Size: 5428 AleI CACnn'nnGTG Cuts at: 1166 2219 1166 Size: 1053 4375 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2237 2284 2561 1184 Size: 1053 47 277 4051 Fragments arranged by size: 4051 1053 277 47 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2294 2326 2294 Size: 32 5396 AlwNI CAG_nnn'CTG Cuts at: 2092 2176 3171 4743 2092 Size: 84 995 1572 2777 Fragments arranged by size: 2777 1572 995 84 ApaLI G'TGCA_C Cuts at: 2222 3485 4641 2222 Size: 1263 1156 3009 Fragments arranged by size: 3009 1263 1156 ApoI r'AATT_y Cuts at: 1493 2249 2342 2362 2657 2737 2963 3115 5224 Size: 756 93 20 295 80 226 152 2109 Cuts at: 5224 1493 Size: 1697 Fragments arranged by size: 2109 1697 756 295 226 152 93 80 20 AseI AT'TA_AT Cuts at: 160 1032 3982 160 Size: 872 2950 1606 Fragments arranged by size: 2950 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 AvaI C'yCGr_G Cuts at: 1870 1943 2603 2649 2753 1870 Size: 73 660 46 104 4545 Fragments arranged by size: 4545 660 104 73 46 BamHI G'GATC_C Cuts at: 2758 3125 2758 Size: 367 5061 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2280 2749 4204 618 Size: 325 217 627 493 469 1455 1842 Fragments arranged by size: 1842 1455 627 493 469 325 217 BanII G_rGCy'C Cuts at: 1391 2523 2697 2747 1391 Size: 1132 174 50 4072 Fragments arranged by size: 4072 1132 174 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4457 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3619 3653 1903 Size: 34 1682 34 3678 Fragments arranged by size: 3678 1682 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3320 4530 3320 Size: 1210 4218 BclI T'GATC_A Cuts at: 1548 1548 Size: 5428 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4039 136 Size: 107 122 71 631 605 2367 1525 Fragments arranged by size: 2367 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5423 5423 Size: 5428 BlpI GC'TnA_GC Cuts at: 2845 2845 Size: 5428 Bme1580I G_kGCm'C Cuts at: 1790 2183 2226 2273 3489 4645 1790 Size: 393 43 47 1216 1156 2573 Fragments arranged by size: 2573 1216 1156 393 47 43 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5428 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3159 4117 442 Size: 416 53 83 399 24 1742 958 1753 Fragments arranged by size: 1753 1742 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5428 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2276 2411 4088 1239 Size: 1037 135 1677 2579 Fragments arranged by size: 2579 1677 1037 135 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2096 2653 3553 4418 4716 2096 Size: 557 900 865 298 2808 Fragments arranged by size: 2808 900 865 557 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4091 882 Size: 617 2592 2219 Fragments arranged by size: 2592 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3219 493 Size: 711 2015 2702 Fragments arranged by size: 2702 2015 711 BsaBI GATnn'nnATC Cuts at: 5422 5422 Size: 5428 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3235 3617 4252 275 Size: 53 83 186 936 1702 382 635 1451 Fragments arranged by size: 1702 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3861 4259 4533 4680 5091 3861 Size: 398 274 147 411 4198 Fragments arranged by size: 4198 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5203 Fragments arranged by size: 5203 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2699 1186 Size: 1513 3915 BseYI C'CCAG_C Cuts at: 2321 2371 4631 5127 2321 Size: 50 2260 496 2622 Fragments arranged by size: 2622 2260 496 50 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2189 1163 Size: 573 453 4402 Fragments arranged by size: 4402 573 453 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2878 3639 4667 1540 Size: 275 186 877 761 1028 2301 Fragments arranged by size: 2301 1028 877 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2523 2697 3489 3574 4645 2226 Size: 297 174 792 85 1071 3009 Fragments arranged by size: 3009 1071 792 297 174 85 BsmI GAATG_Cn' Cuts at: 2939 3032 2939 Size: 93 5335 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2183 2226 2273 2523 2697 2747 3489 Size: 399 393 43 47 250 174 50 742 Cuts at: 3489 3574 4645 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 393 250 174 85 50 47 43 BspEI T'CCGG_A Cuts at: 5091 5091 Size: 5428 BspHI T'CATG_A Cuts at: 3317 5047 3317 Size: 1730 3698 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2014 2783 844 Size: 1170 769 3489 Fragments arranged by size: 3489 1170 769 BsrBI CCG'CTC Cuts at: 1394 1823 3315 1394 Size: 429 1492 3507 Fragments arranged by size: 3507 1492 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2644 3923 4097 65 Size: 1183 1396 1279 174 1396 Fragments arranged by size: 1396 1396 1279 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4072 4259 1700 Size: 110 2262 187 2869 Fragments arranged by size: 2869 2262 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5428 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5428 BssSI C'ACGA_G Cuts at: 3488 4500 3488 Size: 1012 4416 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5428 BstBI TT'CG_AA Cuts at: 4232 4232 Size: 5428 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5428 BstYI r'GATC_y Cuts at: 1972 2758 3125 3510 3527 4979 5065 5077 5423 Size: 786 367 385 17 1452 86 12 346 Cuts at: 5423 1972 Size: 1977 Fragments arranged by size: 1977 1452 786 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3937 Fragments arranged by size: 3937 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4689 Fragments arranged by size: 4689 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2268 2944 3738 3758 2056 Size: 212 676 794 20 3726 Fragments arranged by size: 3726 794 676 212 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5428 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5393 DraI TTT'AAA Cuts at: 2732 2732 Size: 5428 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4435 809 Size: 507 3119 1802 Fragments arranged by size: 3119 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2875 3764 5393 Size: 54 1425 50 275 18 1045 889 1629 Cuts at: 5393 8 Size: 43 Fragments arranged by size: 1629 1425 1045 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2875 1537 Size: 275 1063 4090 Fragments arranged by size: 4090 1063 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3358 1353 Size: 2005 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2170 4015 4389 4535 387 Size: 1064 138 581 1845 374 146 1280 Fragments arranged by size: 1845 1280 1064 581 374 146 138 EcoICRI GAG'CTC Cuts at: 2521 2695 2521 Size: 174 5254 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2238 2276 2411 2435 2496 3491 Size: 393 399 207 38 135 24 61 995 Cuts at: 3491 4088 4875 5143 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 995 787 597 399 393 268 207 135 61 38 24 EcoO109I rG'GnC_Cy Cuts at: 2719 2719 Size: 5428 EcoRI G'AATT_C Cuts at: 2362 2657 2737 3115 2362 Size: 295 80 378 4675 Fragments arranged by size: 4675 378 295 80 FspI TGC'GCA Cuts at: 3934 3934 Size: 5428 HaeII r_GCGC'y Cuts at: 1678 4575 1678 Size: 2897 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2583 2615 4069 4101 4143 Size: 32 135 32 1011 32 1454 32 42 Cuts at: 4143 4175 1373 Size: 32 2626 Fragments arranged by size: 2626 1454 1011 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 2772 3018 669 Size: 988 281 205 629 246 3079 Fragments arranged by size: 3079 988 629 281 246 205 HindIII A'AGCT_T Cuts at: 748 748 Size: 5428 HpaI GTT'AAC Cuts at: 3018 3018 Size: 5428 KpnI G_GTAC'C Cuts at: 2284 2753 2284 Size: 469 4959 MfeI C'AATT_G Cuts at: 3027 3027 Size: 5428 MluI A'CGCG_T Cuts at: 5304 5304 Size: 5428 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 4542 4726 1305 Size: 1032 2205 184 2007 Fragments arranged by size: 2205 2007 1032 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3606 Fragments arranged by size: 3606 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2219 3386 3745 3904 518 Size: 648 1053 1167 359 159 2042 Fragments arranged by size: 2042 1167 1053 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2321 2844 3521 4669 4914 1606 Size: 715 523 677 1148 245 2120 Fragments arranged by size: 2120 1148 715 677 523 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3937 Fragments arranged by size: 3937 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5428 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5428 NotI GC'GGCC_GC Cuts at: 2875 2875 Size: 5428 NspI r_CATG'y Cuts at: 1132 1229 2786 4331 1132 Size: 97 1557 1545 2229 Fragments arranged by size: 2229 1557 1545 97 PasI CC'CwG_GG Cuts at: 1348 2183 1348 Size: 835 4593 PciI A'CATG_T Cuts at: 4327 4327 Size: 5428 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5428 PmeI GTTT'AAAC Cuts at: 2732 2732 Size: 5428 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3497 3529 5010 5042 3497 Size: 32 1481 32 3883 Fragments arranged by size: 3883 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5428 PsiI TTA'TAA Cuts at: 2998 2998 Size: 5428 PstI C_TGCA'G Cuts at: 830 2780 830 Size: 1950 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5428 PvuII CAG'CTG Cuts at: 2321 2321 Size: 5428 RsrII CG'GwC_CG Cuts at: 2157 2157 Size: 5428 SacI G_AGCT'C Cuts at: 2523 2697 2523 Size: 174 5254 SalI G'TCGA_C Cuts at: 1936 2770 1936 Size: 834 4594 SbfI CC_TGCA'GG Cuts at: 2780 2780 Size: 5428 ScaI AGT'ACT Cuts at: 3676 5388 3676 Size: 1712 3716 SfcI C'TryA_G Cuts at: 826 1045 2776 3911 4592 4783 826 Size: 219 1731 1135 681 191 1471 Fragments arranged by size: 1731 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5428 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5428 SmaI CCC'GGG Cuts at: 1872 2651 2755 1872 Size: 779 104 4545 Fragments arranged by size: 4545 779 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2111 2632 3532 4433 4695 820 Size: 197 926 168 521 900 901 262 1553 Fragments arranged by size: 1553 926 901 900 521 262 197 168 SnaBI TAC'GTA Cuts at: 493 3219 493 Size: 2726 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5428 SphI G_CATG'C Cuts at: 1132 2786 1132 Size: 1654 3774 SspI AAT'ATT Cuts at: 5 52 3352 5 Size: 47 3300 2081 Fragments arranged by size: 3300 2081 47 StuI AGG'CCT Cuts at: 1517 2609 1517 Size: 1092 4336 StyI C'CwwG_G Cuts at: 513 1065 2004 2867 513 Size: 552 939 863 3074 Fragments arranged by size: 3074 939 863 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2174 3455 3472 3625 3810 4255 325 Size: 1255 594 1281 17 153 185 445 1498 Fragments arranged by size: 1498 1281 1255 594 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2563 3674 5386 Size: 274 80 33 51 1443 586 1111 1712 Cuts at: 5386 96 Size: 138 Fragments arranged by size: 1712 1443 1111 586 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5388 5420 5388 Size: 32 5396 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5428 XbaI T'CTAG_A Cuts at: 2764 2764 Size: 5428 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5428 XmaI C'CCGG_G Cuts at: 1870 2649 2753 1870 Size: 779 104 4545 Fragments arranged by size: 4545 779 104 XmnI GAAnn'nnTTC Cuts at: 3557 3557 Size: 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3236 276 Size: 53 83 186 936 1702 2468 Fragments arranged by size: 2468 1702 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI