Restriction map for
pFN21AB6930
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanII BbrPI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BmyI BpmI BpuAI BsaAI BsaI
BsaWI BseAI BsiHKAI BsiWI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII DrdI Eam1105I EarI Eco47III
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FbaI FseI FspI HgaI HindIII
HpaI KpnI Ksp632I KspI MaeI
MaeIII MfeI MflI MluI MluNI
MroI MscI MslI MunI MvnI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tsp45I Tth111I XbaI XcmI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AcyI 133 GGCGCC G[GA]^CG[CT]C
AlwI 324 GGATC GGATC(4/5)
Asp700I 354 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII 146 GGACC G^G[AT]CC
BanI 133 GGCGCC G^G[CT][GA]CC
BbeI 133 GGCGCC GGCGC^C
BcnI 130 CCCGG CC^[GC]GG
BlpI 3 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 3 GCTGAGC GC^T[ACGT]AGC
BsaBI 294 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 133 GGCGCC G[GA]^CG[CT]C
BseRI 76 CTCCTC GAGGAG(10/8)
BsgI 661 GTGCAG GTGCAG(16/14)
BsiEI 67 CGGCCG CG[GA][CT]^CG
BsmAI 92 GTCTC GTCTC(1/5)
BsmBI 91 CGTCTC CGTCTC(1/5)
BsmFI 145 GGGAC GGGAC(10/14)
BspHI 95 TCATGA T^CATGA
BstNI 36 CCTGG CC^[AT]GG
CelII 3 GCTGAGC GC^T[ACGT]AGC
DpnII 325 GATC ^GATC
DraII 145 GGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 416 CCATGG C^C[GA][CT]GG
EaeI 67 CGGCCG [CT]^GGCC[GA]
EagI 67 CGGCCG C^GGCCG
EclXI 67 CGGCCG C^GGCCG
Eco52I 67 CGGCCG C^GGCCG
EcoO109I 145 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 548 GAATTC G^AATTC
EcoRII 36 CCTGG ^CC[AT]GG
EcoT14I 416 CCATGG C^C[AT][AT]GG
HaeII 133 GGCGCC [GA]GCGC^[CT]
Hin1I 133 GGCGCC G[GA]^CG[CT]C
HincII 567 GTCAAC GT[CT]^[GA]AC
HindII 567 GTCAAC GT[CT]^[GA]AC
HphI 244 GGTGA GGTGA(8/7)
KasI 133 GGCGCC G^GCGCC
MamI 294 GATAACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 325 GATC ^GATC
MvaI 36 CCTGG CC^[AT]GG
NarI 133 GGCGCC GG^CGCC
NciI 130 CCCGG CC^[GC]GG
NcoI 416 CCATGG C^CATGG
NdeII 325 GATC ^GATC
PflMI 30 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 145 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 95 TCATGA T^CATGA
Sau3AI 325 GATC ^GATC
SfoI 133 GGCGCC GGC^GCC
SmlI 490 CTTGAG C^T[CT][GA]AG
StyI 416 CCATGG C^C[AT][AT]GG
TaqI 167 TCGA T^CGA
TspRI 151 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 167 TCGA T^CGA
Van91I 30 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 354 GAATAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 358 AAATTC [GA]^AATT[CT]
548 GAATTC
AfaI 261 GTAC GT^AC
283 GTAC
ApoI 358 AAATTC [GA]^AATT[CT]
548 GAATTC
BsrI 150 CCAGT ACTGG(1/-1)
286 CCAGT
BssKI 36 CCTGG ^CC[ACGT]GG
130 CCCGG
BstF5I 403 GGATG GGATG(2/0)
420 GGATG
CfoI 134 GCGC GCG^C
155 GCGC
FauI 62 CCCGC CCCGC(4/6)
158 CCCGC
FokI 403 GGATG GGATG(9/13)
420 GGATG
HhaI 134 GCGC GCG^C
155 GCGC
HinP1I 134 GCGC G^CGC
155 GCGC
HinfI 72 GATTC G^A[ACGT]TC
232 GATTC
MaeII 32 ACGT A^CGT
90 ACGT
MseI 266 TTAA T^TAA
310 TTAA
MspA1I 9 CCGCTG C[AC]G^C[GT]G
103 CCGCTG
NlaIII 96 CATG CATG^
417 CATG
PstI 55 CTGCAG CTGCA^G
194 CTGCAG
RsaI 261 GTAC GT^AC
283 GTAC
ScrFI 36 CCTGG CC^[ACGT]GG
130 CCCGG
SfaNI 622 GATGC GCATC(5/9)
161 GCATC
SfcI 55 CTGCAG C^T[GA][CT]AG
194 CTGCAG
TfiI 72 GATTC G^A[AT]TC
232 GATTC
Tru9I 266 TTAA T^TAA
310 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsrFI Cac8I Cfr10I Cfr13I
DdeI Fnu4HI HaeIII HapII HpaII
ItaI MboII MnlI MspI MwoI
NaeI NgoMIV NlaIV Sau96I TseI
Tsp509I