Restriction map for
pFN21AB6930
(Linear) (Six-base) MAPSORT of: KIBB6930.seq Check: 4392 from: 1 to: 684
KIBB6930.orf
With 182 enzymes: SgfI *
April 24, 2009 13:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 684
Size: 547 137
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 684
Size: 40 644
ApoI r'AATT_y
Cuts at: 0 358 548 684
Size: 358 190 136
Fragments arranged by size:
358 190 136
BanI G'GyrC_C
Cuts at: 0 133 684
Size: 133 551
BbeI G_GCGC'C
Cuts at: 0 137 684
Size: 137 547
BlpI GC'TnA_GC
Cuts at: 0 4 684
Size: 4 680
BmgBI CAC'GTC
Cuts at: 0 33 91 684
Size: 33 58 593
Fragments arranged by size:
593 58 33
BmrI ACTGGGnnnn_n'
Cuts at: 0 144 684
Size: 144 540
Bpu10I CC'TnA_GC
Cuts at: 0 43 684
Size: 43 641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 684
Size: 511 173
BsaBI GATnn'nnATC
Cuts at: 0 298 684
Size: 298 386
BsaHI Gr'CG_yC
Cuts at: 0 134 684
Size: 134 550
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 684
Size: 67 617
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 684
Size: 682 2
BsiEI CG_ry'CG
Cuts at: 0 70 684
Size: 70 614
BsmBI CGTCTCn'nnnn_
Cuts at: 0 97 684
Size: 97 587
BspHI T'CATG_A
Cuts at: 0 95 684
Size: 95 589
BsrFI r'CCGG_y
Cuts at: 0 20 65 136 684
Size: 20 45 71 548
Fragments arranged by size:
548 71 45 20
BtgI C'CryG_G
Cuts at: 0 416 684
Size: 416 268
EaeI y'GGCC_r
Cuts at: 0 67 684
Size: 67 617
EagI C'GGCC_G
Cuts at: 0 67 684
Size: 67 617
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 547 684
Size: 547 137
EcoO109I rG'GnC_Cy
Cuts at: 0 146 684
Size: 146 538
EcoRI G'AATT_C
Cuts at: 0 548 684
Size: 548 136
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 412 684
Size: 412 272
HaeII r_GCGC'y
Cuts at: 0 137 684
Size: 137 547
HincII GTy'rAC
Cuts at: 0 569 684
Size: 569 115
KasI G'GCGC_C
Cuts at: 0 133 684
Size: 133 551
MspA1I CmG'CkG
Cuts at: 0 11 105 684
Size: 11 94 579
Fragments arranged by size:
579 94 11
NaeI GCC'GGC
Cuts at: 0 22 67 138 684
Size: 22 45 71 546
Fragments arranged by size:
546 71 45 22
NarI GG'CG_CC
Cuts at: 0 134 684
Size: 134 550
NcoI C'CATG_G
Cuts at: 0 416 684
Size: 416 268
NgoMIV G'CCGG_C
Cuts at: 0 20 65 136 684
Size: 20 45 71 548
Fragments arranged by size:
548 71 45 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 684
Size: 36 648
PpuMI rG'GwC_Cy
Cuts at: 0 146 684
Size: 146 538
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 428 460 684
Size: 428 32 224
Fragments arranged by size:
428 224 32
PstI C_TGCA'G
Cuts at: 0 59 198 684
Size: 59 139 486
Fragments arranged by size:
486 139 59
SanDI GG'GwC_CC
Cuts at: 0 146 684
Size: 146 538
SfcI C'TryA_G
Cuts at: 0 55 194 684
Size: 55 139 490
Fragments arranged by size:
490 139 55
SfoI GGC'GCC
Cuts at: 0 135 684
Size: 135 549
SmlI C'TyrA_G
Cuts at: 0 490 684
Size: 490 194
StyI C'CwwG_G
Cuts at: 0 416 684
Size: 416 268
TatI w'GTAC_w
Cuts at: 0 260 684
Size: 260 424
XmnI GAAnn'nnTTC
Cuts at: 0 358 684
Size: 358 326
Enzymes that do cut:
AcuI AlfI ApoI BanI BbeI BlpI BmgBI BmrI
Bpu10I BpuEI BsaBI BsaHI BseRI BsgI BsiEI BsmBI
BspHI BsrFI BtgI EaeI EagI Eco57MI EcoO109I EcoRI
FalI HaeII HincII KasI MspA1I NaeI NarI NcoI
NgoMIV PflMI PpuMI PsrI PstI SanDI SfcI SfoI
SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmtI BplI BpmI BsaI BsaAI BsaWI BsaXI BseYI
BsiHKAI BsiWI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoICRI EcoNI EcoRV FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI Tth111I XbaI XcmI XhoI XmaI ZraI